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Malachi Griffith edited this page Apr 30, 2015 · 41 revisions

#0-ii. Citation and description of published materials

This tutorial is accompanied by a publication. If you find the materials here or in that paper useful please cite the following:

Malachi Griffith*, Jason R. Walker, Nicholas C. Spies, Benjamin J. Ainscough, Obi L. Griffith*. Informatics for RNA-seq: A web resource for analysis on the cloud. In preparation.

*To whom correspondence should be addressed: E-mail: mgriffit[AT]genome.wustl.edu, ogriffit[AT]genome.wustl.edu

All materials associated with this publication, including high resolution and original figure files, supplementary tables, etc. are available here: https://github.com/griffithlab/rnaseq_tutorial

This includes all figures and supplementary tables.

  • Figure 1 - An overview of the central dogma of molecular biology
  • Figure 2 - RNA-seq data generation
  • Figure 3 - RNA-seq library fragmentation and size selection strategies that influence interpretation and analysis
  • Figure 4 - RNA-seq library enrichment strategies that influence interpretation and analysis
  • Figure 5 - RNA-seq analysis flow-chart
  • Figure 6 - Comparison of stranded and unstranded RNA-seq library methods and their influence on interpretation and analysis
  • Supplementary Table 1 - Tools for RNA-seq analysis
  • Supplementary Table 2 - RNA-seq analysis techniques
  • Supplementary Table 3 - Concepts in sample preparation and library construction that can influence study design, analysis and interpretation
  • Supplementary Table 4 - Strand related settings for RNA-seq tools that must be adjusted to account for library construction strategy
  • Supplementary Table 5 - Standard file formats and tool-specific files used in RNA-seq analysis
  • Supplementary Table 6 - Common RNA-seq questions and their answers
  • Supplementary Table 7 - Resources for RNA-seq analysis
  • Supplementary Table 8 - RNA-seq workshops and online tutorials

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