Download WZY_Time_Lapse.ijm and drop it to your imagej. The example image Calcium_Oscillation_ExampleImage.zip can be directly droped into your imagej.
- It is better to use Fiji (https://fiji.sc/) instead of imagej, since Fiji no need to set up all plugin that this macro needs. Fiji is just imagej(https://fiji.sc/), so if it can not work in imagej then please Fiji(https://fiji.sc/).
- Copy this WZY_Time_Lapse.ijm file to your the plugins directory of your Fiji software.
- Make ROI for the entire image and for each cell (or just target cells).
Welcome to contact me, I am glad to help for making some customized imagej plugins.