Port prot2pept to typescript

bin/prot2pept.ts

@@ -0,0 +1,6 @@
+#!/usr/bin/env node
+
+import { Prot2pept } from '../lib/commands/prot2pept.js';
+
+const command = new Prot2pept();
+command.run();

lib/commands/prot2pept.ts

@@ -0,0 +1,80 @@
+import { createInterface } from 'node:readline';
+import { BaseCommand } from './base_command.js';
+
+export class Prot2pept extends BaseCommand {
+
+  readonly description = `The prot2pept command splits each protein sequence into a list of peptides according to a given cleavage-pattern. The command expects a list of protein sequences that are passed to standard input.
+
+The input should have either one protein sequence per line or contain a FASTA formatted list of protein sequences. FASTA headers are preserved in the output, so that peptides can be bundled per protein sequence.
+`;
+
+  constructor(options?: { exitOverride?: boolean, suppressOutput?: boolean, args?: string[] }) {
+    super(options);
+
+    this.program
+      .summary("Splits each protein sequence into a list of peptides.")
+      .description(this.description)
+      .option("-p, --pattern <regex>", "specify cleavage-pattern (regex) as the pattern after which the next peptide will be cleaved. By default, it will create tryptic peptides.", "([KR])([^P])")
+  }
+
+  /**
+   * Performance note: Just as with peptfilter, this implementation can be made faster by using line events instead of
+   * async iterators.
+   */
+  async run() {
+    this.parseArguments();
+
+    let pattern;
+    try {
+      pattern = new RegExp(this.program.opts().pattern, "g");
+    } catch (e) {
+      this.program.error(`Your pattern was invalid: ${(e as Error).message}`);
+      //process.exit(1);
+    }
+
+    let fasta = false;
+    let protein = [];
+
+    // buffering output makes a big difference in performance
+    let output = [];
+    let i = 0;
+
+    for await (const line of createInterface({ input: process.stdin })) {
+      if (i === 0 && line.startsWith(">")) {
+        fasta = true;
+      }
+
+      i++;
+
+      if (fasta) { // if we're in fasta mode, a protein could be split over multiple lines
+        if (line.startsWith(">")) { // if we encounter a new header, process the previous protein and output the current header
+          if (protein.length > 0) {
+            output.push(Prot2pept.splitProtein(protein.join(""), pattern));
+          }
+          output.push(line.trimEnd());
+          protein = [];
+        } else {
+          protein.push(line.trimEnd());
+        }
+      } else { // if we're not in fasta mode, each line is a protein sequence
+        output.push(Prot2pept.splitProtein(line.trimEnd(), pattern));
+      }
+
+      if (i % 1000 === 0) {
+        output.push(""); //add a newline at the end of the buffer without additional string copy
+        process.stdout.write(output.join("\n"));
+        output = [];
+      }
+    }
+
+    if (fasta) { // if in fasta mode, process the last protein
+      output.push(Prot2pept.splitProtein(protein.join(""), pattern));
+    }
+    output.push("");
+    process.stdout.write(output.join("\n"));
+  }
+
+  static splitProtein(line: string, pattern: RegExp): string {
+    return line.replaceAll(pattern, "$1\n$2").replaceAll(pattern, "$1\n$2").replaceAll("\n\n", "\n");
+  }
+}

package.json

@@ -9,6 +9,7 @@
   "type": "module",
   "bin": {
     "peptfilter": "./bin/peptfilter.js",
+    "prot2pept": "./bin/prot2pept.js",
     "uniprot": "./bin/uniprot.js"
   },
   "scripts": {
@@ -17,6 +18,7 @@
     "test": "NODE_OPTIONS='--experimental-vm-modules --no-warnings' yarn run jest",
     "typecheck": "yarn tsc --skipLibCheck --noEmit",
     "peptfilter": "yarn run tsx bin/peptfilter.ts",
+    "prot2pept": "yarn run tsx bin/prot2pept.ts",
     "uniprot": "yarn run tsx bin/uniprot.ts"
   },
   "dependencies": {

tests/commands/prot2pept.test.ts

@@ -0,0 +1,124 @@
+import { Prot2pept } from '../../lib/commands/prot2pept';
+import { jest } from '@jest/globals';
+import * as mock from 'mock-stdin';
+
+let output: string[];
+let error: string[];
+// eslint-disable-next-line @typescript-eslint/no-unused-vars
+const writeSpy = jest
+  .spyOn(process.stdout, "write")
+  .mockImplementation((data: unknown) => { output.push(data as string); return true; });
+const errorSpy = jest
+  .spyOn(process.stderr, "write")
+  .mockImplementation((data: unknown) => { error.push(data as string); return true; });
+
+beforeEach(() => {
+  output = [];
+  error = [];
+});
+
+test('test single line input 1', async () => {
+  const stdin = mock.stdin();
+
+  const command = new Prot2pept();
+  const run = command.run();
+
+  stdin.send("AALTERAALTERPAALTER\n");
+  stdin.end();
+
+  await run;
+
+  expect(errorSpy).toHaveBeenCalledTimes(0);
+  expect(output.join("").trimEnd()).toBe("AALTER\nAALTERPAALTER");
+});
+
+test('test single line input 2', async () => {
+  const stdin = mock.stdin();
+
+  const command = new Prot2pept();
+  const run = command.run();
+
+  stdin.send("KRKPR\n");
+  stdin.end();
+
+  await run;
+
+  expect(errorSpy).toHaveBeenCalledTimes(0);
+  expect(output.join("").trimEnd()).toBe("K\nR\nKPR");
+});
+
+test('test multi line input', async () => {
+  const stdin = mock.stdin();
+
+  const command = new Prot2pept();
+  const run = command.run();
+
+  stdin.send("AALTERAALTERPAALTER\n");
+  stdin.send("AALTERAA\n");
+  stdin.end();
+
+  await run;
+
+  expect(errorSpy).toHaveBeenCalledTimes(0);
+  expect(output.join("").trimEnd()).toBe("AALTER\nAALTERPAALTER\nAALTER\nAA");
+});
+
+test('test fasta input 1', async () => {
+  const stdin = mock.stdin();
+
+  const command = new Prot2pept();
+  const run = command.run();
+
+  stdin.send(">AKA\nAALTERAALTERPAALTER\n");
+  stdin.end();
+
+  await run;
+
+  expect(errorSpy).toHaveBeenCalledTimes(0);
+  expect(output.join("").trimEnd()).toBe(">AKA\nAALTER\nAALTERPAALTER");
+});
+
+test('test fasta input 2', async () => {
+  const stdin = mock.stdin();
+
+  const command = new Prot2pept();
+  const run = command.run();
+
+  stdin.send(">AKA\nAAL\nT\nERAALTER\nP\nAALTER\n");
+  stdin.end();
+
+  await run;
+
+  expect(errorSpy).toHaveBeenCalledTimes(0);
+  expect(output.join("").trimEnd()).toBe(">AKA\nAALTER\nAALTERPAALTER");
+});
+
+test('test fasta input 3', async () => {
+  const stdin = mock.stdin();
+
+  const command = new Prot2pept();
+  const run = command.run();
+
+  stdin.send(">AKA\nAAL\nT\n>\nERAALTER\nP\nAALTER");
+  stdin.end();
+
+  await run;
+
+  expect(errorSpy).toHaveBeenCalledTimes(0);
+  expect(output.join("").trimEnd()).toBe(">AKA\nAALT\n>\nER\nAALTERPAALTER");
+});
+
+test('test custom pattern', async () => {
+  const stdin = mock.stdin();
+
+  const command = new Prot2pept({ args: ["--pattern", "([KR])([^A])"] });
+  const run = command.run();
+
+  stdin.send("AALTERAALTERPAALTER\n");
+  stdin.end();
+
+  await run;
+
+  expect(errorSpy).toHaveBeenCalledTimes(0);
+  expect(output.join("").trimEnd()).toBe("AALTERAALTER\nPAALTER");
+});

tsconfig.json

@@ -8,5 +8,6 @@
     "module": "NodeNext",
     "strict": true,
     "resolveJsonModule": true,
+    "target": "esnext"
   }
 }