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updated zenodo files and fixed snippet issue
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timonschlegel committed Jun 23, 2024
1 parent fabc4d4 commit 50b713e
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Expand Up @@ -25,3 +25,7 @@ items:
src: url
ext: auto
info: https://zenodo.org/records/11369811
- url: https://zenodo.org/api/records/11369811/files/BAM_500-PBMC.bam/content
src: url
ext: auto
info: https://zenodo.org/records/11369811
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Expand Up @@ -151,7 +151,7 @@ SnapATAC2 requires 3 input files for the standard pathway of processing:
> > <hands-on-title>fragment file</hands-on-title>
> > 1. Import the file `BAM_500-PBMC` from [Zenodo]({{ page.zenodo_link }}) or from the shared data library
> > ```
> > {{ page.zenodo_link }}/files/atac_pbmc_5k_nextgem_fragments.tsv
> > {{ page.zenodo_link }}/files/BAM_500-PBMC.bam
> > ```
> > - This dataset contains mapped reads in the `BAM` format.
> > - It was generated by following the tutorial ["Pre-processing of 10X Single-Cell ATAC-seq Datasets"]( {% link topics/single-cell/tutorials/scatac-preprocessing-tenx/tutorial.md %} ) until the output of {% tool [Map with BWA-MEM](toolshed.g2.bx.psu.edu/repos/devteam/bwa/bwa_mem/0.7.18) %}
Expand Down Expand Up @@ -885,7 +885,9 @@ To manually annotate the *Leiden* clusters, we will need to perform multiple ste
> 7. Rename the generated file to `Cell type annotation` and {% icon galaxy-eye %} inspect the file to check if the replacement was successful
> - check if the datatype is set to `tabular`
> - you may need to change the datatype manually
>
> {% snippet faqs/galaxy/datasets_change_datatype.md datatype="tabular"%}
>
> 8. {% tool [Manipulate AnnData](toolshed.g2.bx.psu.edu/repos/iuc/anndata_manipulate/anndata_manipulate/0.10.3+galaxy0) %} with the following parameters:
> - {% icon param-file %} *"Annotated data matrix"*: `Anndata 5k PBMC gene_matrix magic UMAP`
> - *"Function to manipulate the object"*: `Add new annotation(s) for observations or variables`
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