This repository contains Perl scripts for analysis of deep sequencing data for a cysteine scanning mutagenesis library.
Please refer to the file 'Analysis_Workflow.docx' for detailed descriptions of the scripts and instructions on how to run them. Please refer to Najar et al., Structure 25 (3), 395-406. (2017) PMID: 28132782 for additional details.
Created by Shruti Khare, Prof. Raghavan Varadarajan's Lab, Indian Institute of Science on March 10th, 2017. For queries contact [email protected] OR [email protected]
Citation: Najar TA, Khare S, Pandey R, Gupta SK, Varadarajan R. Mapping Protein Binding Sites and Conformational Epitopes Using Cysteine Labeling and Yeast Surface Display. Structure. 2017 Mar 7;25(3):395-406. doi: 10.1016/j.str.2016.12.016. PubMed PMID: 28132782.