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Sequence Analysis

Rajan edited this page Aug 9, 2023 · 15 revisions

Sequence Alignment

ViralMSA: Reference-guided multiple sequence alignment of viral genomes.

Aliview: Alignment viewer and editor

MAFFT: Tool to create multiple sequence alignment based on progressive alignment algorithm.

Mauve: Tool to create multiple sequence alignment based on progressive alignment and alignment display.

ClustalW: Multiple Sequence Alignment.

NGS analysis

Samtools: Used for manipulating NGS data

BWA: Used for mapping NGS reads against large reference genome

BWA-MEM2: Used for mapping NGS reads against large reference genome (Faster than BWA)

wgsim: Simulate sequence reads from a reference genome

ART: Next-generation sequencing read simulator

Sambamba: Processing of NGS alignment formats

fastp: Preprocessing for FastQ file.

seqtk: Processing sequences in the FASTA or FASTQ format

BBMap: Short read aligner for DNA and RNA-seq data

Pan Genome Pipeline

Roary: Calculates the pan genome and core genome.

Genome-wide association study (GWAS)

Scoary: Pan-genome wide association studies (Pan-GWAS).

SNP Analysis

SnpEff: Genomic variant annotations and functional effect prediction

Snippy: Finds SNPs between a haploid reference genome and sequence isolates.

snipit: summarise snps relative to your reference sequence.

Genome Annotation

Prokka: Rapid prokaryotic genome annotation.

CRISPRCasFinder: Detect CRISPR repeats and cas genes from the bacterial genomic sequence

RNAmmer: Predict and annotate ribosomal RNA

Ribodetector: Detect and remove rRNA sequences from metagenomeic, metatranscriptomic, and ncRNA sequencing data

Motif Finder

FIMO: Searches a set of sequences for occurrences of known motifs.

Comparative genomics

OrthoVenn: Comparison and annotation of orthologous gene clusters.

Easyfig: Make genome comparison figure

QUAST: Genome assembly evaluation tool

Recombination

ClonalFrameML: Inference of recombination in bacterial genomes.

Gubbins: Recombination predictions in bacterial genomes.

Antimicrobial resistance and lineage prediction

Mykrobe: Prediction of Antimicrobial resistance and Lineage in Mycobacterium tuberculosis.

RD-Analyzer: In silico region of difference (RD) analysis of Mycobacterium tuberculosis complex.

Mobile Genetic Elements (MGEs) Analysis

MobileElementFinder: Identify Mobile Genetic Elements (MGEs) in Bacterial Genome

PHAST (PHAge Search Tool): Identify, annotate and graphically display prophage sequences within bacterial genomes or plasmids (Online).

ISEScan: Identify IS (Insertion Sequence) elements in genome.

AI models for Genomics

Kipoi: Model zoo for genomics