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Hierarchical clustering through menu “Structure” → “Compute hierarchy on dataset” Agglomerative clustering with average linkage (WPGMA) and cosine distance is used
Adaptive range modes for y-axis in profile charts (“Set Zoom …” actions in toolbar)
“Copy to clipboard” for plot snapshots (MacOS: “⌘+⇧+C”, others: “Print” key) The clipboard content is suitable both for vector drawing (e.g. Illustrator, Inkscape) and raster graphics (e.g. Photoshop, GIMP)
When slicing a dataset, normalization can be applied again Default setting depends on normalization of source dataset
Pruning of tiny clusters can be disabled for hierarchy cut and Mean Shift computations
Visualization tab also works when only two dimensions available
Allow reading huge marker sets of unknown proteins The limit of 500 proteins is applied to proteins in the project, others are ignored
Use “MOUSE” as default species when none given
Bug Fixes
Fix logarithm applied to raw feature data This lead to wrong data being stored in project file, observed as a wrong scale when loading logarithmic data. To solve the problem for existing project files, remove the affected dataset and import it again.
Fix weight display in Feature Weighting when one antibody gets full weight This error occurred typically with very few antibodies and markers
Fix within-cluster order changed when loading previously stored Mean Shift result
Fix protein selection overridden by hovering over cluster label even when cursor locked
Fix hierarchy cut granularity toolbar not always showing granularity
Fix wrong tooltips in Visualization tab
Fix memory leaks
Fix sliced object copy
Improvements
Redesigned main toolbars and per-tab toolbars, toolbars now user-adjustable
Use left mouse button for selection/browsing in Feature Weighting
Improved rendering quality for plots exported in PNG format
Show species next to name in protein menu title
Avoid clutter with minor tick labels in small profile plots and BNMS plots
Default hierarchy cut granularity increased to 10
Display a message when no protein from marker file found in project
Parallelize feature normalization computations
Project files now store whether a dataset was normalized
Project files store whether hierarchy cuts or Mean Shift clusters were pruned
Development
Update QtCharts patches to newer, incompatible QtCharts version
Replace Qt calls deprecated in Qt 5.15
Improve computation task architecture for future enhancements
Introduce re-usable plot appearance component for further enhancements