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Changed scratch3 to scratch
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Gammerdinger authored May 8, 2024
1 parent 24eec38 commit aeed707
Showing 1 changed file with 6 additions and 6 deletions.
12 changes: 6 additions & 6 deletions lessons/07_variant_calling.md
Original file line number Diff line number Diff line change
Expand Up @@ -277,10 +277,10 @@ Next, we need to add our variables:
REFERENCE_SEQUENCE=/n/groups/hbctraining/variant_calling/reference/GRCh38.p7.fa
REFERENCE_DICTIONARY=`echo ${REFERENCE_SEQUENCE%fa}dict`
NORMAL_SAMPLE_NAME=syn3_normal
NORMAL_BAM_FILE=/n/scratch3/users/${USER:0:1}/${USER}/variant_calling/alignments/${NORMAL_SAMPLE_NAME}_GRCh38.p7.coordinate_sorted.bam
NORMAL_BAM_FILE=/n/scratch/users/${USER:0:1}/${USER}/variant_calling/alignments/${NORMAL_SAMPLE_NAME}_GRCh38.p7.coordinate_sorted.bam
TUMOR_SAMPLE_NAME=syn3_tumor
TUMOR_BAM_FILE=/n/scratch3/users/${USER:0:1}/${USER}/variant_calling/alignments/${TUMOR_SAMPLE_NAME}_GRCh38.p7.coordinate_sorted.bam
VCF_OUTPUT_FILE=/n/scratch3/users/${USER:0:1}/${USER}/variant_calling/vcf_files/mutect2_${NORMAL_SAMPLE_NAME}_${TUMOR_SAMPLE_NAME}_GRCh38.p7-raw.vcf
TUMOR_BAM_FILE=/n/scratch/users/${USER:0:1}/${USER}/variant_calling/alignments/${TUMOR_SAMPLE_NAME}_GRCh38.p7.coordinate_sorted.bam
VCF_OUTPUT_FILE=/n/scratch/users/${USER:0:1}/${USER}/variant_calling/vcf_files/mutect2_${NORMAL_SAMPLE_NAME}_${TUMOR_SAMPLE_NAME}_GRCh38.p7-raw.vcf
```

<details>
Expand Down Expand Up @@ -408,10 +408,10 @@ module load gatk/4.1.9.0
REFERENCE_SEQUENCE=/n/groups/hbctraining/variant_calling/reference/GRCh38.p7.fa
REFERENCE_DICTIONARY=`echo ${REFERENCE_SEQUENCE%fa}dict`
NORMAL_SAMPLE_NAME=syn3_normal
NORMAL_BAM_FILE=/n/scratch3/users/${USER:0:1}/${USER}/variant_calling/alignments/${NORMAL_SAMPLE_NAME}_GRCh38.p7.coordinate_sorted.bam
NORMAL_BAM_FILE=/n/scratch/users/${USER:0:1}/${USER}/variant_calling/alignments/${NORMAL_SAMPLE_NAME}_GRCh38.p7.coordinate_sorted.bam
TUMOR_SAMPLE_NAME=syn3_tumor
TUMOR_BAM_FILE=/n/scratch3/users/${USER:0:1}/${USER}/variant_calling/alignments/${TUMOR_SAMPLE_NAME}_GRCh38.p7.coordinate_sorted.bam
VCF_OUTPUT_FILE=/n/scratch3/users/${USER:0:1}/${USER}/variant_calling/vcf_files/mutect2_${NORMAL_SAMPLE_NAME}_${TUMOR_SAMPLE_NAME}_GRCh38.p7-raw.vcf
TUMOR_BAM_FILE=/n/scratch/users/${USER:0:1}/${USER}/variant_calling/alignments/${TUMOR_SAMPLE_NAME}_GRCh38.p7.coordinate_sorted.bam
VCF_OUTPUT_FILE=/n/scratch/users/${USER:0:1}/${USER}/variant_calling/vcf_files/mutect2_${NORMAL_SAMPLE_NAME}_${TUMOR_SAMPLE_NAME}_GRCh38.p7-raw.vcf
# Run MuTect2
gatk Mutect2 \
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