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Update snipit to v1.6
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@wm75
wm75 committed Nov 15, 2024
1 parent b537658 commit ea6dbf6
Showing 1 changed file with 103 additions and 68 deletions.
171 changes: 103 additions & 68 deletions tools/snipit/snipit.xml
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Original file line number Diff line number Diff line change
@@ -1,7 +1,20 @@
<tool id="snipit" name="snipit" version="@TOOL_VERSION@+galaxy0">
<description>Summarise snps relative to a reference sequence</description>
<macros>
<token name="@TOOL_VERSION@">1.2</token>
<token name="@TOOL_VERSION@">1.6</token>
<xml name="ref_select" token_help="">
<conditional name="ref">
<param name="select" type="select" label="The reference sequence ..."
help="@HELP@">
<option value="first">is the first sequence in the input</option>
<option value="by_id">should be picked via its ID</option>
</param>
<when value="first" />
<when value="by_id">
<param name="id" argument="--reference" type="text" label="ID of reference sequence" />
</when>
</conditional>
</xml>
<xml name="handle_background" tokens="format">
<when value="@FORMAT@">
<param argument="--solid-background" name="transparent_background" type="boolean" truevalue="" falsevalue="--solid-background" label="Plot on transparent background?" />
Expand All @@ -17,15 +30,39 @@
<version_command>snipit -v</version_command>
<command detect_errors="exit_code"><![CDATA[
snipit '$alignment'
#if str($ref.select) == 'by_id'
--sequence-type $mode.sequence_type
#if str($mode.sequence_type) == 'nt'
$mode.cds_mode
#end if
#if str($mode.ref.select) == 'by_id'
-r '$ref.id'
#end if
#if not str($mode.colouring.palette)
--recombi-mode --recombi-references '$mode.colouring.parent1,$mode.colouring.parent2'
#else
-c $mode.colouring.palette
#end if
$pos_restrict.show_indels
--ambig-mode $pos_restrict.ambig_mode
#if len($pos_restrict.include_positions)
--include-positions
#for $p in $pos_restrict.include_positions
'$p.pos'
#end for
#end if
#if len($pos_restrict.exclude_positions)
--exclude-positions
#for $p in $pos_restrict.exclude_positions
'$p.pos'
#end for
#end if
#if str($style.labels.choose)
-l '$style.labels.source'
#if str($style.labels.choose) == 'custom_csv'
--l-header '${style.labels.names_column},${style.labels.labels_column}'
#end if
#end if
$style.position_labels
-f $plot.format
#if str($plot.format) in ['png', 'svg', 'tiff']
$plot.transparent_background
Expand All @@ -39,51 +76,69 @@
#end if
$dims.size_option
$style.flip_vertical
$show_indels
#if len($pos_restrict.include_positions)
--include-positions
#for $p in $pos_restrict.include_positions
'$p.pos'
#end for
#end if
#if len($pos_restrict.exclude_positions)
--exclude-positions
#for $p in $pos_restrict.exclude_positions
'$p.pos'
#end for
#end if
$pos_restrict.exclude_ambig_pos
$style.sort.order
#if str($style.sort.order) == '--sort-by-mutation-number'
$style.sort.high_to_low
#else if str($style.sort.order) == '--sort-by-mutations'
#echo ','.join([str(p['pos']) for p in $style.sort.positions])
$style.sort.high_to_low
#end if
#if not str($style.colouring.palette)
--recombi-mode --recombi-references '$style.colouring.parent1,$style.colouring.parent2'
#else
-c $style.colouring.palette
#end if
]]></command>
<environment_variables>
<environment_variable name="MPLCONFIGDIR">.matplotlib</environment_variable>
</environment_variables>
<inputs>
<param name="alignment" format="fasta" type="data" label="Input alignment fasta file" />
<conditional name="ref">
<param name="select" type="select" label="The reference sequence ..."
help="Please note that you will HAVE to specify the reference explicitly via its ID if you choose the 'recombinant mutations colouring' scheme and in this case the reference can NOT be one of the recombinant parents.">
<option value="first">is the first sequence in the input</option>
<option value="by_id">should be picked via its ID</option>
<conditional name="mode">
<param argument="--sequence-type" type="select" label="Type of input">
<option value="nt">DNA sequence alignment</option>
<option value="aa">Protein sequence alignment</option>
</param>
<when value="first" />
<when value="by_id">
<param name="id" argument="--reference" type="text" label="ID of reference sequence" />
<when value="nt">
<expand macro="ref_select" help="Please note that you will HAVE to specify the reference explicitly via its ID if you choose the 'recombinant mutations colouring' scheme and in this case the reference can NOT be one of the recombinant parents." />
<param argument="--cds-mode" type="boolean" truevalue="--cds-mode" falsevalue="" label="Perform coding sequence alignment check?" help="By selecting this option you are indicating that your input is an alignment of coding sequences. The tool will check that all sequence lengths are multiples of three and will fail if that is not the case." />
<conditional name="colouring">
<param name="palette" argument="--colour-palette" type="select" label="Plotted Mutations colouring scheme" help="Mutations can be coloured by base change or, alternatively, by their presence/absence in any of two parent sequences in the alignment (recombinant mode).">
<option value="primary">primary</option>
<option value="classic" selected="true">classic</option>
<option value="classic_extended">classic_extended (only scheme defining colours for ambiguous bases)</option>
<option value="purine-pyrimidine">purine-pyrimidine</option>
<option value="greyscale">greyscale</option>
<option value="wes">wes</option>
<option value="verity">verity</option>
<option value="">recombinant mutations colouring</option>
</param>
<when value="primary" />
<when value="classic" />
<when value="classic_extended" />
<when value="purine-pyrimidine" />
<when value="greyscale" />
<when value="wes" />
<when value="verity" />
<when value="">
<param name="parent1" type="text" label="Sequence ID of first recombinant parent sequence in alignment" />
<param name="parent2" type="text" label="Sequence ID of second recombinant parent sequence in alignment" />
</when>
</conditional>
</when>
<when value="aa">
<expand macro="ref_select" />
<conditional name="colouring">
<param name="palette" argument="--colour-palette" type="select" label="Plotted Mutations colouring scheme" help="The only currently available colour scheme for protein sequences is 'ugene', which defines colours for the 20 AAs and all AA ambiguity symbols.">
<option value="ugene">ugene</option>
</param>
<when value="ugene" />
</conditional>
</when>
</conditional>
<section name="pos_restrict" title="Restrict genome positions to be plotted" expanded="false">
<repeat name='include_positions' title="Restrict plot to specific regions/positions" min="0" default="0">
<section name="pos_restrict" title="Restrict alignment positions to be plotted" expanded="true">
<param argument="--show-indels" type="boolean" truevalue="--show-indels" falsevalue="" label="Include insertion and deletion mutations" />
<param argument="--ambig-mode" type="select" label="Plotting of positions with ambiguity symbols">
<option value="all">Treat ambiguity symbols like mutations; plot all such positions (all)</option>
<option value="snps" selected="true">Show ambiguity symbols for positions with mutations in other sequences (snps)</option>
<option value="exclude">Never plot positions that involve ambiguous symbols (exclude)</option>
</param>
<repeat name="include_positions" title="Restrict plot to specific regions/positions" min="0" default="0">
<param argument="--include-positions" name="pos" type="text" label="Include position/region" help="A range (closed, inclusive; one-indexed) or specific position to include in the output, e.g. '100-150' or '100'">
<validator type="regex" message="Either a single position, e.g. 8347, or an interval like 8000-8500 is required">[0-9]+(-[0-9]+)*$</validator>
</param>
Expand All @@ -93,7 +148,6 @@
<validator type="regex" message="Either a single position, e.g. 8347, or an interval like 8000-8500 is required">[0-9]+(-[0-9]+)*$</validator>
</param>
</repeat>
<param argument="--exclude-ambig-pos" type="boolean" truevalue="--exclude-ambig-pos" falsevalue="" label="Exclude ambig positions?" help="Exclude positions with an ambiguous base in any sequences; considered after '--include-positions'." />
</section>
<section name="style" title="Customize rendering of samples and mutations" expanded="true">
<conditional name="labels">
Expand All @@ -112,27 +166,7 @@
<param name="labels_column" type="text" label="Name of the column with desired labels" />
</when>
</conditional>
<conditional name="colouring">
<param name="palette" type="select" value="classic" label="Mutations colouring scheme" help="Mutations can be coloured by base change or, alternatively, by their presence/absence in any of two parent sequences in the alignment (recombinant mode).">
<option value="primary">primary</option>
<option value="classic" selected="true">classic</option>
<option value="purine-pyrimidine">purine-pyrimidine</option>
<option value="greyscale">greyscale</option>
<option value="wes">wes</option>
<option value="verity">verity</option>
<option value="">recombinant mutations colouring</option>
</param>
<when value="primary" />
<when value="classic" />
<when value="purine-pyrimidine" />
<when value="greyscale" />
<when value="wes" />
<when value="verity" />
<when value="">
<param name="parent1" type="text" label="Sequence ID of first recombinant parent sequence in alignment" />
<param name="parent2" type="text" label="Sequence ID of second recombinant parent sequence in alignment" />
</when>
</conditional>
<param name="position_labels" type="boolean" truevalue="" falsevalue="--remove-site-text" checked="true" label="Label plotted sites with position" />
<conditional name="sort">
<param name="order" type="select" label="Order of samples in the plot">
<option value="">Go with default</option>
Expand Down Expand Up @@ -176,7 +210,6 @@
<option value="" selected="true">scale</option>
</param>
</section>
<param argument="--show-indels" type="boolean" truevalue="--show-indels" falsevalue="" label="Try to plot indels" help="This is newly introduced functionality for which you may encounter bugs still." />
<param argument="--write-snps" type="boolean" truevalue="-s" falsevalue="" label="Write out SNPs?" help="Produces extra CSV output with lists of SNPs per sequence in the alignment if enabled." />
</inputs>
<outputs>
Expand Down Expand Up @@ -207,9 +240,11 @@
</test>
<test expect_num_outputs="1">
<param name="alignment" value="input.fasta" />
<conditional name="ref">
<param name="select" value="by_id" />
<param name="id" value="USA_2" />
<conditional name="mode">
<conditional name="ref">
<param name="select" value="by_id" />
<param name="id" value="USA_2" />
</conditional>
</conditional>
<section name="style">
<param name="flip_vertical" value="true" />
Expand All @@ -225,11 +260,11 @@
</test>
<test expect_num_outputs="1">
<param name="alignment" value="input.fasta" />
<section name="style">
<conditional name="mode">
<conditional name="colouring">
<param name="palette" value="verity" />
</conditional>
</section>
</conditional>
<conditional name="plot">
<param name="format" value="jpg" />
<param name="transparent_background" value="true" />
Expand All @@ -239,6 +274,7 @@
<test expect_num_outputs="1">
<param name="alignment" value="input.fasta" />
<section name="pos_restrict">
<param name="ambig_mode" value="exclude" />
<repeat name="include_positions">
<param name="pos" value="1-10000" />
</repeat>
Expand All @@ -248,7 +284,6 @@
<repeat name="exclude_positions">
<param name="pos" value="1000-2000" />
</repeat>
<param name="exclude_ambig_pos" value="true" />
</section>
<section name="style">
<conditional name="sort">
Expand All @@ -272,7 +307,7 @@
<assert_command>
<has_text text="--include-positions '1-10000' '10001-30000'" />
<has_text text="--exclude-positions '1000-2000'" />
<has_text text="--exclude-ambig-pos" />
<has_text text="--ambig-mode exclude" />
<has_text text="--sort-by-mutations 3,1,2" />
<has_text text="--high-to-low" />
</assert_command>
Expand All @@ -289,17 +324,17 @@
</test>
<test expect_num_outputs="1">
<param name="alignment" value="input.fasta" />
<conditional name="ref">
<param name="select" value="by_id" />
<param name="id" value="reference" />
</conditional>
<section name="style">
<conditional name="mode">
<conditional name="ref">
<param name="select" value="by_id" />
<param name="id" value="reference" />
</conditional>
<conditional name="colouring">
<param name="palette" value="" />
<param name="parent1" value="USA_1" />
<param name="parent2" value="USA_5" />
</conditional>
</section>
</conditional>
<conditional name="plot">
<param name="format" value="svg" />
</conditional>
Expand Down Expand Up @@ -343,7 +378,7 @@
<help><![CDATA[
**What it does**
Snipit finds the SNPs relative to a reference in a multiple sequence alignment and highlights these changes as a figure.
Snipit finds mutations relative to a reference in a multiple sequence alignment and presnts these changes in a nice overview plot.
]]>
</help>
<citations>
Expand Down

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