Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Update labs microgalaxy manual #265

Merged
merged 4 commits into from
Dec 19, 2024
Merged
Changes from all commits
Commits
File filter

Filter by extension

Filter by extension

Conversations
Failed to load comments.
Loading
Jump to
Jump to file
Failed to load files.
Loading
Diff view
Diff view
121 changes: 51 additions & 70 deletions communities/microgalaxy/lab/sections/1_data_import_and_preparation.yml
Original file line number Diff line number Diff line change
@@ -1,15 +1,34 @@
id: data
title: Data import and preparation
title: Getting started
tabs:
- id: tools
title: Tools
- id: data-import
title: Data import
heading_md: >
Common tools are listed here, or search for more in the full tool panel to the left.
Common tools that allow for data import.
content:
- title_md: Import data to Galaxy
description_md: >
Standard upload of data to Galaxy, from your computer or from the web.
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=upload1"
- title_md: Download data from NCBI GenBank/RefSeq
description_md: >
Download sequences from GenBank/RefSeq by accession through the NCBI ENTREZ API
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fncbi_acc_download%2Fncbi_acc_download"
- title_md: Download raw reads from NCBI SRA
description_md: >
Faster Download and Extract Reads in FASTQ format from NCBI SRA
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsra_tools%2Ffasterq_dump"
- title_md: Download run data from EBI Metagenomics (MGnify)
description_md: >
This tool downloads data related to a run in EBI Metagenomics database
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Febi_metagenomics_run_downloader%2Febi_metagenomics_run_downloader"


- id: highlight-tools
title: Highlight tools
heading_md: >
Flagship tools for microbial research
content:
- title_md: <code>FastQC</code> - sequence quality reports
description_md: >
<p>
Expand All @@ -25,86 +44,48 @@ tabs:
- title_md: <code>FastP</code> - sequence quality reports, trimming & filtering
description_md: >
<p>
Faster run than FastQC, this tool can also trim reads and filter by quality.
Commonly used tool for trimming and quality filter. Also provides quality metrics.
</p>
inputs:
- datatypes:
- fastq
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Ffastp%2Ffastp"
- title_md: <code>NanoPlot</code> - visualize Oxford Nanopore data
- title_md: <code>Kraken2</code> - Kraken2 assign taxonomic labels to sequencing reads
description_md: >
<p>
A plotting suite for Oxford Nanopore sequencing data and alignments.
Kraken2 assign taxonomic labels to sequencing reads.
</p>
inputs:
- datatypes:
- fastq
- fasta
- vcf_bgzip
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fnanoplot%2Fnanoplot"
- title_md: <code>GenomeScope</code> - estimate genome size
description_md: >
<p>
A set of metrics and graphs to visualize genome size and complexity prior to assembly.
</p>
inputs:
- datatypes:
- tabular
label: Output from <code>Meryl</code> or <code>Jellyfish histo</code>
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fgenomescope%2Fgenomescope"
- title_md: <code>Meryl</code> - count kmers
description_md: >
<p>
Prepare kmer count histogram for input to GenomeScope.
</p>
inputs:
- datatypes:
- fastq
- fasta
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fmeryl%2Fmeryl"

- id: workflows
title: Workflows
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fkraken2%2Fkraken2"

- id: learning-pathways
title: Learning pathways
heading_md: >
A workflow is a series of Galaxy tools that have been linked together to perform a specific analysis. You can use and customize the example workflows below.
<a href="https://galaxyproject.org/learn/advanced-workflow/" target="_blank">Learn more.</a>
Connected tutorials to train you to perform microbial data analysis.
content:
- title_md: Data QC
- title_md: Introduction to Galaxy and Sequence analysis
description_md: >
<p>
Report statistics from sequencing reads. <br> <br> Tools: <code>nanoplot</code> <code>fastqc</code> <code>multiqc</code>
</p>
button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=222"
view_link: https://workflowhub.eu/workflows/222
view_tip: View in WorkflowHub
button_tip: Import to Galaxy AU
- title_md: Kmer counting to estimate genome size
New to Galaxy and/or the field of genomics? In this learning pathway, you will learn how to use Galaxy for analysis, and will be guided through the most common first steps of any genome analysis; quality control and a mapping or assembly of your genomic sequences.
button_link: https://training.galaxyproject.org/training-material/learning-pathways/intro-to-galaxy-and-genomics.html
heading_md: >
Connected tutorials to train you to perform microbial data analysis.
- title_md: Detection of AMR genes in bacterial genomes
description_md: >
<p>
Estimates genome size and heterozygosity based on counts of kmers. <br> <br> Tools: <code>meryl</code> <code>genomescope</code>
</p>
button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=223"
view_link: https://workflowhub.eu/workflows/223
view_tip: View in WorkflowHub
button_tip: Import to Galaxy AU
- title_md: Trim and filter reads
This learning path aims to teach you the basic steps to detect and check Antimicrobial resistance (AMR) genes in bacterial genomes using Galaxy.
button_link: https://training.galaxyproject.org/training-material/learning-pathways/amr-gene-detection.html
- title_md: Clinical metaproteomics workflows within Galaxy
description_md: >
<p>
Trims and filters raw sequence reads according to specified settings. <br> <br> Tools: <code>fastp</code>
</p>
button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=224"
view_link: https://workflowhub.eu/workflows/224
view_tip: View in WorkflowHub
button_tip: Import to Galaxy AU

- id: tutorials
title: Tutorials
heading_md: >

content: []

- id: faq
title: FAQ
heading_md: >

content: []
This learning path aims to teach you the basics of how to perform metaproteomics analysis of the clinical data within the Galaxy platform. You will learn how to use Galaxy for analysis and will be guided through the most common first steps of any metaproteomics database generation to searching the database, verifying the proteins/peptides, and data analysis.
button_link: https://training.galaxyproject.org/training-material/learning-pathways/clinical-metaproteomics.html
- title_md: Genome annotation for prokaryotes
description_md: >
Learn how to annotate a prokaryotic genome sequence: find the position and function of genes, and even set up a manual curation environment with Apollo.
button_link: https://training.galaxyproject.org/training-material/learning-pathways/genome-annotation-prokaryote.html
- title_md: Metagenomics data processing and analysis for microbiome
description_md: >
This learning path aims to teach you the basics of Galaxy and analysis of metagenomics data. You will learn how to use Galaxy for analysis, and will be guided through the common steps of microbiome data analysis: quality control, taxonomic profiling, taxonomic binning, assembly, functional profiling, and also some applications
button_link: https://training.galaxyproject.org/training-material/learning-pathways/metagenomics.html