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Added informative comments in the 'Alignment filtering' section of 'm…
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…apping.yaml' file.
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microbiomix committed Jan 29, 2024
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Expand Up @@ -963,6 +963,14 @@ BWA_threads: 10
BWA_memory: 45
# Alignment filtering
# -------------------
# msamtools_filter_length: Default 50 works well for ILLUMINA 2x150bp.
# For shorter sequencing, e.g. 2x75bp, please reduce accordingly.
# alignment_identity: Default 97 works well for MAGs that are dereped at 99%.
# For refgenomes, modify it according to the derep level of your refgenomes.
# For gene catalog, modify it to match the derep level of the gene catalog (usually 95%).
# MIN_mapped_reads: Default 2 is designed for low-to-medium sequencing depth.
# Increase according to depth (e.g., set to 10 when depth is above 5Gb).
msamtools_filter_length: 50
alignment_identity: 97
MIN_mapped_reads: 2
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