ImageJ/Fiji Macro Set dedicated to cytometric analysis of n-dimensional images containing at least one channel of a nuclear dye, as well as a cellular counterstain and a mitochondria marker. It returns nuclear, cellular and mitochondia shape descriptors and intensity parameters. Build on Cellblocks_v19.ijm (https://github.com/DeVosLab/CellBlocks).
Please cite these papers if you are using this script in your research:
- De Vos WH, Van Neste L, Dieriks B, Joss GH, Van Oostveldt P. High content image cytometry in the context of subnuclear organization. Cytometry A. 2010 Jan;77(1):64-75. doi: 10.1002/cyto.a.20807. PMID: 19821512.
- De Puysseleyr L, De Puysseleyr K, Vanrompay D, De Vos WH. Quantifying the growth of chlamydia suis in cell culture using high-content microscopy. Microsc Res Tech. 2017 Apr;80(4):350-356. doi: 10.1002/jemt.22799. Epub 2016 Nov 12. PMID: 27862609.
- Sieprath T, Corne T, Robijns J, Koopman WJH, De Vos WH. Cellular Redox Profiling Using High-content Microscopy. J Vis Exp. 2017 May 14;(123):55449. doi: 10.3791/55449. PMID: 28570523; PMCID: PMC5607941.