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settings.sh
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settings.sh
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# fasta file of assembly to scaffold
fafile=FAFILE
# fastq file with long reads
fqfile=FQFILE
# working directory
odir=ODIR
#bin directory: where the executables are
bindir=$MYSMISDIR/smissv-bin
#which samtools
mysamtools=`which samtools`
# running mode
debug=0
########### FAKE-MATES PARAMETERS: ###########
# min length of long reads
minlen=3000
# length of fake mates
fakelen=2000
# distance between one fake mate pairs and the next
step=200
########### ALIGNMENT PARAMETERS: ###########
# which aligner to use (bwa, smalt)
aligner=bwa
# bwa
mybwa=`which bwa`
# smalt
mysmalt=`which smalt`
# max threads:
nodes=25
# minimum smith-waterman alignment score to report a hit
mapscore=50
# Alignment scores and penalties
match=2
subst=-1
gapopen=-1
gapext=-1
########### SCAFFOLDING PARAMETERS: ###########
# minimum numbers of edges:
min_edge=5.0
num_sigma=`awk "BEGIN {print ($min_edge)/2.5}"`