diff --git a/404.html b/404.html index 4894707..0fc0c3a 100644 --- a/404.html +++ b/404.html @@ -32,7 +32,7 @@
diff --git a/CODE_OF_CONDUCT.html b/CODE_OF_CONDUCT.html index 7f21b16..4503049 100644 --- a/CODE_OF_CONDUCT.html +++ b/CODE_OF_CONDUCT.html @@ -17,7 +17,7 @@ diff --git a/articles/index.html b/articles/index.html index ff8039b..dfbda3d 100644 --- a/articles/index.html +++ b/articles/index.html @@ -17,7 +17,7 @@ diff --git a/articles/tximeta.html b/articles/tximeta.html index ce5bbfa..11ca949 100644 --- a/articles/tximeta.html +++ b/articles/tximeta.html @@ -33,7 +33,7 @@ @@ -74,7 +74,7 @@vignettes/tximeta.Rmd
tximeta.Rmd
edb <- retrieveDb(se)
## loading existing TxDb created: 2023-10-09 18:51:07
+## loading existing TxDb created: 2024-01-12 13:23:25
## Loading required package: GenomicFeatures
## Loading required package: AnnotationDbi
@@ -439,7 +439,7 @@Add exons per transcript
-se.exons <- addExons(se)
+## loading existing TxDb created: 2023-10-09 18:51:07
## loading existing TxDb created: 2024-01-12 13:23:25
## generating exon ranges
@@ -478,7 +478,7 @@rowRanges(se.exons)[[1]]
Easy summarization to gene-leveltx_ids.
-gse <- summarizeToGene(se)
+## loading existing TxDb created: 2023-10-09 18:51:07
## loading existing TxDb created: 2024-01-12 13:23:25
## obtaining transcript-to-gene mapping from database
## generating gene ranges
@@ -580,7 +580,7 @@## gene ranges assigned by total range of isoforms, see `assignRanges`
Differential expression analysisThe following code chunk demonstrates how to build a DESeqDataSet and begin a differential expression analysis.
@@ -594,7 +594,7 @@-suppressPackageStartupMessages(library(DESeq2)) +
suppressPackageStartupMessages(library(DESeq2)) # here there is a single sample so we use ~1. # expect a warning that there is only a single sample... suppressWarnings({dds <- DESeqDataSet(gse, ~1)})
Differential expression analysistximeta if these were generated during quantification.
--library(fishpond) +
library(fishpond) y <- se # y <- scaleInfReps(y) # y <- labelKeep(y) @@ -624,7 +624,7 @@
Differential expression analysis
y <- se # rename the object to 'y' -library(fishpond) +library(fishpond) # if inferential replicates existed in the data, # analysis would begin with: # @@ -635,9 +635,9 @@
Differential expression analysis
-gse <- summarizeToGene(se, countsFromAbundance="lengthScaledTPM")
+## loading existing TxDb created: 2023-10-09 18:51:07
## loading existing TxDb created: 2024-01-12 13:23:25
-## obtaining transcript-to-gene mapping from database
+## loading existing gene ranges created: 2023-10-09 18:51:11
## loading existing gene ranges created: 2024-01-12 13:23:28
## gene ranges assigned by total range of isoforms, see `assignRanges`
## summarizing abundance
@@ -714,9 +714,9 @@## summarizing counts
Additional metadatastr(metadata(se)[["tximetaInfo"]])
+## $ importTime: POSIXct[1:1], format: "2024-01-12 13:23:16"## List of 3 ## $ version :Classes 'package_version', 'numeric_version' hidden list of 1 -## ..$ : int [1:3] 1 19 10 +## ..$ : int [1:3] 1 21 2 ## $ type : chr "salmon" -## $ importTime: POSIXct[1:1], format: "2023-10-09 18:50:57"
## Named chr [1:13] "TxDb" "GenomicFeatures" ... @@ -914,7 +914,7 @@
Linked transcriptomes release="98", genome="BDGP6.22", fasta=fastaFTP, gtf=gtfPath, jsonFile=jsonFile)
+## writing linkedTxome to /tmp/Rtmp0CoyeU/Dm.BDGP6.22.98.plus_salmon-0.14.1.json
## writing linkedTxome to /tmp/RtmpPFrOWi/Dm.BDGP6.22.98.plus_salmon-0.14.1.json
## saving linkedTxome in bfc
After running
makeLinkedTxome
, the connection between this Salmon index (and its checksum) with the sources is saved @@ -1023,13 +1023,13 @@Clear linkedTxomes
## # A tibble: 7 × 10 ## rid rname create_time access_time rpath rtype fpath last_modified_time etag ## <chr> <chr> <chr> <chr> <chr> <chr> <chr> <dbl> <chr> -## 1 BFC1 link… 2023-10-09… 2023-10-09… /tmp… rela… 9296… NA NA -## 2 BFC2 Dros… 2023-10-09… 2023-10-09… /tmp… rela… 9296… NA NA -## 3 BFC3 txpR… 2023-10-09… 2023-10-09… /tmp… rela… 9292… NA NA -## 4 BFC4 exon… 2023-10-09… 2023-10-09… /tmp… rela… 9294… NA NA -## 5 BFC5 gene… 2023-10-09… 2023-10-09… /tmp… rela… 9293… NA NA -## 6 BFC6 Dros… 2023-10-09… 2023-10-09… /tmp… rela… 9294… NA NA -## 7 BFC7 txpR… 2023-10-09… 2023-10-09… /tmp… rela… 9293… NA NA +## 1 BFC1 link… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA +## 2 BFC2 Dros… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA +## 3 BFC3 txpR… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA +## 4 BFC4 exon… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA +## 5 BFC5 gene… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA +## 6 BFC6 Dros… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA +## 7 BFC7 txpR… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA ## # ℹ 1 more variable: expires <dbl>
bfcremove(bfc, bfcquery(bfc, "linkedTxomeTbl")$rid)
@@ -1037,12 +1037,12 @@ Clear linkedTxomes## # A tibble: 6 × 10
## rid rname create_time access_time rpath rtype fpath last_modified_time etag
## <chr> <chr> <chr> <chr> <chr> <chr> <chr> <dbl> <chr>
-## 1 BFC2 Dros… 2023-10-09… 2023-10-09… /tmp… rela… 9296… NA NA
-## 2 BFC3 txpR… 2023-10-09… 2023-10-09… /tmp… rela… 9292… NA NA
-## 3 BFC4 exon… 2023-10-09… 2023-10-09… /tmp… rela… 9294… NA NA
-## 4 BFC5 gene… 2023-10-09… 2023-10-09… /tmp… rela… 9293… NA NA
-## 5 BFC6 Dros… 2023-10-09… 2023-10-09… /tmp… rela… 9294… NA NA
-## 6 BFC7 txpR… 2023-10-09… 2023-10-09… /tmp… rela… 9293… NA NA
+## 1 BFC2 Dros… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA
+## 2 BFC3 txpR… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA
+## 3 BFC4 exon… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA
+## 4 BFC5 gene… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA
+## 5 BFC6 Dros… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA
+## 6 BFC7 txpR… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA
## # ℹ 1 more variable: expires <dbl>
## 1
## found matching linked transcriptome:
## [ LocalEnsembl - Drosophila melanogaster - release 98 ]
-## loading existing TxDb created: 2023-10-09 18:51:25
-## loading existing transcript ranges created: 2023-10-09 18:51:25
+## loading existing TxDb created: 2024-01-12 13:23:40
+## loading existing transcript ranges created: 2024-01-12 13:23:40
## Warning in checkAssays2Txps(assays, txps):
##
## Warning: the annotation is missing some transcripts that were quantified.
@@ -1104,13 +1104,13 @@ Clear linkedTxomes again## # A tibble: 7 × 10
## rid rname create_time access_time rpath rtype fpath last_modified_time etag
## <chr> <chr> <chr> <chr> <chr> <chr> <chr> <dbl> <chr>
-## 1 BFC2 Dros… 2023-10-09… 2023-10-09… /tmp… rela… 9296… NA NA
-## 2 BFC3 txpR… 2023-10-09… 2023-10-09… /tmp… rela… 9292… NA NA
-## 3 BFC4 exon… 2023-10-09… 2023-10-09… /tmp… rela… 9294… NA NA
-## 4 BFC5 gene… 2023-10-09… 2023-10-09… /tmp… rela… 9293… NA NA
-## 5 BFC6 Dros… 2023-10-09… 2023-10-09… /tmp… rela… 9294… NA NA
-## 6 BFC7 txpR… 2023-10-09… 2023-10-09… /tmp… rela… 9293… NA NA
-## 7 BFC8 link… 2023-10-09… 2023-10-09… /tmp… rela… 9297… NA NA
+## 1 BFC2 Dros… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA
+## 2 BFC3 txpR… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA
+## 3 BFC4 exon… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA
+## 4 BFC5 gene… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA
+## 5 BFC6 Dros… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA
+## 6 BFC7 txpR… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA
+## 7 BFC8 link… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA
## # ℹ 1 more variable: expires <dbl>
bfcremove(bfc, bfcquery(bfc, "linkedTxomeTbl")$rid)
@@ -1118,12 +1118,12 @@ Clear linkedTxomes again## # A tibble: 6 × 10
## rid rname create_time access_time rpath rtype fpath last_modified_time etag
## <chr> <chr> <chr> <chr> <chr> <chr> <chr> <dbl> <chr>
-## 1 BFC2 Dros… 2023-10-09… 2023-10-09… /tmp… rela… 9296… NA NA
-## 2 BFC3 txpR… 2023-10-09… 2023-10-09… /tmp… rela… 9292… NA NA
-## 3 BFC4 exon… 2023-10-09… 2023-10-09… /tmp… rela… 9294… NA NA
-## 4 BFC5 gene… 2023-10-09… 2023-10-09… /tmp… rela… 9293… NA NA
-## 5 BFC6 Dros… 2023-10-09… 2023-10-09… /tmp… rela… 9294… NA NA
-## 6 BFC7 txpR… 2023-10-09… 2023-10-09… /tmp… rela… 9293… NA NA
+## 1 BFC2 Dros… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA
+## 2 BFC3 txpR… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA
+## 3 BFC4 exon… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA
+## 4 BFC5 gene… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA
+## 5 BFC6 Dros… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA
+## 6 BFC7 txpR… 2024-01-12… 2024-01-12… /tmp… rela… 1253… NA NA
## # ℹ 1 more variable: expires <dbl>
## ─ Session info ───────────────────────────────────────────────────────────────
## setting value
-## version R version 4.3.1 (2023-06-16)
+## version R version 4.3.2 (2023-10-31)
## os Ubuntu 22.04.3 LTS
## system x86_64, linux-gnu
## ui X11
@@ -1211,146 +1211,145 @@ Session info## collate en_US.UTF-8
## ctype en_US.UTF-8
## tz UTC
-## date 2023-10-09
+## date 2024-01-12
## pandoc 3.1.1 @ /usr/local/bin/ (via rmarkdown)
##
## ─ Packages ───────────────────────────────────────────────────────────────────
## package * version date (UTC) lib source
## abind 1.4-5 2016-07-21 [1] RSPM (R 4.3.0)
-## AnnotationDbi * 1.62.2 2023-07-02 [1] Bioconductor
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-## BiocIO 1.10.0 2023-04-25 [1] Bioconductor
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-## BiocParallel 1.34.2 2023-05-22 [1] Bioconductor
-## BiocVersion 3.17.1 2022-11-04 [2] Bioconductor
-## biomaRt 2.56.1 2023-06-09 [1] Bioconductor
-## Biostrings 2.68.1 2023-05-16 [1] Bioconductor
+## AnnotationDbi * 1.64.1 2023-11-03 [1] Bioconductor
+## AnnotationFilter 1.26.0 2023-10-24 [1] Bioconductor
+## AnnotationHub 3.10.0 2023-10-24 [1] Bioconductor
+## Biobase * 2.62.0 2023-10-24 [1] Bioconductor
+## BiocFileCache * 2.10.1 2023-10-26 [1] Bioconductor
+## BiocGenerics * 0.48.1 2023-11-01 [1] Bioconductor
+## BiocIO 1.12.0 2023-10-24 [1] Bioconductor
+## BiocManager 1.30.22 2023-08-08 [2] CRAN (R 4.3.2)
+## BiocParallel 1.36.0 2023-10-24 [1] Bioconductor
+## BiocVersion 3.18.1 2023-11-15 [2] Bioconductor
+## biomaRt 2.58.0 2023-10-24 [1] Bioconductor
+## Biostrings 2.70.1 2023-10-25 [1] Bioconductor
## bit 4.0.5 2022-11-15 [1] RSPM (R 4.3.0)
## bit64 4.0.5 2020-08-30 [1] RSPM (R 4.3.0)
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## blob 1.2.4 2023-03-17 [1] RSPM (R 4.3.0)
-## bslib 0.5.1 2023-08-11 [2] RSPM (R 4.3.0)
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## cachem 1.0.8 2023-05-01 [2] RSPM (R 4.3.0)
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## colorspace 2.1-0 2023-01-23 [1] RSPM (R 4.3.0)
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-## DESeq2 * 1.40.2 2023-06-23 [1] Bioconductor
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## purrr 1.0.2 2023-08-10 [2] RSPM (R 4.3.0)
## R6 2.5.1 2021-08-19 [2] RSPM (R 4.3.0)
-## ragg 1.2.5 2023-01-12 [2] RSPM (R 4.3.0)
+## ragg 1.2.7 2023-12-11 [2] RSPM (R 4.3.0)
## rappdirs 0.3.3 2021-01-31 [2] RSPM (R 4.3.0)
## Rcpp 1.0.11 2023-07-06 [2] RSPM (R 4.3.0)
-## RCurl 1.98-1.12 2023-03-27 [1] RSPM (R 4.3.0)
-## remotes 2.4.2.9000 2023-10-06 [1] Github (r-lib/remotes@8875171)
-## restfulr 0.0.15 2022-06-16 [1] RSPM (R 4.3.0)
+## RCurl 1.98-1.13 2023-11-02 [1] RSPM (R 4.3.0)
+## remotes 2.4.2.9000 2024-01-12 [1] Github (r-lib/remotes@6fe8836)
+## restfulr 0.0.15 2022-06-16 [1] RSPM (R 4.3.2)
## rjson 0.2.21 2022-01-09 [1] RSPM (R 4.3.0)
-## rlang 1.1.1 2023-04-28 [2] RSPM (R 4.3.0)
+## rlang 1.1.2 2023-11-04 [2] RSPM (R 4.3.0)
## rmarkdown 2.25 2023-09-18 [2] RSPM (R 4.3.0)
-## rprojroot 2.0.3 2022-04-02 [2] RSPM (R 4.3.0)
-## Rsamtools 2.16.0 2023-04-25 [1] Bioconductor
-## RSQLite 2.3.1 2023-04-03 [1] RSPM (R 4.3.0)
-## rtracklayer 1.60.1 2023-08-15 [1] Bioconductor
-## S4Arrays 1.0.6 2023-08-30 [1] Bioconductor
-## S4Vectors * 0.38.2 2023-09-22 [1] Bioconductor
-## sass 0.4.7 2023-07-15 [2] RSPM (R 4.3.0)
-## scales 1.2.1 2022-08-20 [1] RSPM (R 4.3.0)
+## Rsamtools 2.18.0 2023-10-24 [1] Bioconductor
+## RSQLite 2.3.4 2023-12-08 [1] RSPM (R 4.3.0)
+## rtracklayer 1.62.0 2023-10-24 [1] Bioconductor
+## S4Arrays 1.2.0 2023-10-24 [1] Bioconductor
+## S4Vectors * 0.40.2 2023-11-23 [1] Bioconductor 3.18 (R 4.3.2)
+## sass 0.4.8 2023-12-06 [2] RSPM (R 4.3.0)
+## scales 1.3.0 2023-11-28 [1] RSPM (R 4.3.0)
## sessioninfo 1.2.2 2021-12-06 [2] RSPM (R 4.3.0)
-## shiny 1.7.5 2023-08-12 [2] RSPM (R 4.3.0)
-## SingleCellExperiment 1.22.0 2023-04-25 [1] Bioconductor
-## stringi 1.7.12 2023-01-11 [2] RSPM (R 4.3.0)
-## stringr 1.5.0 2022-12-02 [2] RSPM (R 4.3.0)
-## SummarizedExperiment * 1.30.2 2023-06-06 [1] Bioconductor
+## shiny 1.8.0 2023-11-17 [2] RSPM (R 4.3.0)
+## SingleCellExperiment 1.24.0 2023-10-24 [1] Bioconductor
+## SparseArray 1.2.3 2023-12-25 [1] Bioconductor 3.18 (R 4.3.2)
+## statmod 1.5.0 2023-01-06 [1] RSPM (R 4.3.0)
+## stringi 1.8.3 2023-12-11 [2] RSPM (R 4.3.0)
+## stringr 1.5.1 2023-11-14 [2] RSPM (R 4.3.0)
+## SummarizedExperiment * 1.32.0 2023-10-24 [1] Bioconductor
## svMisc 1.2.3 2021-10-11 [1] RSPM (R 4.3.0)
-## systemfonts 1.0.4 2022-02-11 [2] RSPM (R 4.3.0)
-## textshaping 0.3.6 2021-10-13 [2] RSPM (R 4.3.0)
+## systemfonts 1.0.5 2023-10-09 [2] RSPM (R 4.3.0)
+## textshaping 0.3.7 2023-10-09 [2] RSPM (R 4.3.0)
## tibble 3.2.1 2023-03-20 [2] RSPM (R 4.3.0)
## tidyselect 1.2.0 2022-10-10 [1] RSPM (R 4.3.0)
-## tximeta * 1.19.10 2023-10-09 [1] Bioconductor
-## tximport 1.28.0 2023-04-25 [1] Bioconductor
+## tximeta * 1.21.2 2024-01-12 [1] Bioconductor
+## tximport 1.30.0 2023-10-24 [1] Bioconductor
## urlchecker 1.0.1 2021-11-30 [2] RSPM (R 4.3.0)
## usethis * 2.2.2 2023-07-06 [2] RSPM (R 4.3.0)
-## utf8 1.2.3 2023-01-31 [2] RSPM (R 4.3.0)
-## vctrs 0.6.3 2023-06-14 [2] RSPM (R 4.3.0)
-## withr 2.5.1 2023-09-26 [2] RSPM (R 4.3.0)
-## xfun 0.40 2023-08-09 [2] RSPM (R 4.3.0)
-## XML 3.99-0.14 2023-03-19 [1] RSPM (R 4.3.0)
-## xml2 1.3.5 2023-07-06 [2] RSPM (R 4.3.0)
+## utf8 1.2.4 2023-10-22 [2] RSPM (R 4.3.0)
+## vctrs 0.6.5 2023-12-01 [2] RSPM (R 4.3.0)
+## withr 2.5.2 2023-10-30 [2] RSPM (R 4.3.0)
+## xfun 0.41 2023-11-01 [2] RSPM (R 4.3.0)
+## XML 3.99-0.16 2023-11-29 [1] RSPM (R 4.3.0)
+## xml2 1.3.6 2023-12-04 [2] RSPM (R 4.3.0)
## xtable 1.8-4 2019-04-21 [2] RSPM (R 4.3.0)
-## XVector 0.40.0 2023-04-25 [1] Bioconductor
-## yaml 2.3.7 2023-01-23 [2] RSPM (R 4.3.0)
-## zlibbioc 1.46.0 2023-04-25 [1] Bioconductor
+## XVector 0.42.0 2023-10-24 [1] Bioconductor
+## yaml 2.3.8 2023-12-11 [2] RSPM (R 4.3.0)
+## zlibbioc 1.48.0 2023-10-24 [1] Bioconductor
##
## [1] /__w/_temp/Library
## [2] /usr/local/lib/R/site-library
diff --git a/authors.html b/authors.html
index d999ba9..6469e95 100644
--- a/authors.html
+++ b/authors.html
@@ -17,7 +17,7 @@
NEWS.md
+
assignRanges
that takes either “range” (default) or “abundant”, and determines the ranges that are attached to the SE (rowRanges). Note that this new argument does not affect the data aggregation at all (counts and abundance are summarized to gene by tximport
). The default behavior of summarizeToGene() returns ranges that correspond to the range
of the isoforms of the gene, that is the leftmost basepair to the rightmost basepair of any isoform. The non-default “abundant” instead returns the range of the most abundant isoform in the data, averaging over samples. Information about the choice of range is included in mcols
diff --git a/pkgdown.yml b/pkgdown.yml
index 7fd43fc..588da55 100644
--- a/pkgdown.yml
+++ b/pkgdown.yml
@@ -3,7 +3,7 @@ pkgdown: 2.0.7
pkgdown_sha: ~
articles:
tximeta: tximeta.html
-last_built: 2023-10-09T18:50Z
+last_built: 2024-01-12T13:22Z
urls:
reference: https://thelovelab.github.io/tximeta/reference
article: https://thelovelab.github.io/tximeta/articles
diff --git a/reference/addCDS.html b/reference/addCDS.html
index 4fad316..92652cb 100644
--- a/reference/addCDS.html
+++ b/reference/addCDS.html
@@ -30,7 +30,7 @@