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I am following the "Analysis, visualization, and integration of spatial datasets with Seurat" vignette. I am at the section "Subset out anatomical regions" when I run the first subset command, I get the following:
> cortex <- subset(brain, idents = c(1, 2, 3, 4, 6, 7))
Warning messages:
1: Not validating Centroids objects
2: Not validating Centroids objects
3: Not validating FOV objects
4: Not validating FOV objects
5: Not validating FOV objects
6: Not validating FOV objects
7: Not validating FOV objects
8: Not validating FOV objects
9: Not validating Seurat objects
Then, when running a further subset, it throws an error:
> cortex <- subset(cortex, anterior1_imagerow > 400 | anterior1_imagecol < 150, invert = TRUE)
Error in `FetchData()`:
! None of the requested variables were found:
I have seen a few other people having issues with subset although I can't seem to find a solution that works for me in this situation.
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I am following the "Analysis, visualization, and integration of spatial datasets with Seurat" vignette. I am at the section "Subset out anatomical regions" when I run the first subset command, I get the following:
Then, when running a further subset, it throws an error:
I have seen a few other people having issues with subset although I can't seem to find a solution that works for me in this situation.
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