diff --git a/lessons/07_variant_calling.md b/lessons/07_variant_calling.md index e31a69e..7e7c450 100644 --- a/lessons/07_variant_calling.md +++ b/lessons/07_variant_calling.md @@ -277,10 +277,10 @@ Next, we need to add our variables: REFERENCE_SEQUENCE=/n/groups/hbctraining/variant_calling/reference/GRCh38.p7.fa REFERENCE_DICTIONARY=`echo ${REFERENCE_SEQUENCE%fa}dict` NORMAL_SAMPLE_NAME=syn3_normal -NORMAL_BAM_FILE=/n/scratch3/users/${USER:0:1}/${USER}/variant_calling/alignments/${NORMAL_SAMPLE_NAME}_GRCh38.p7.coordinate_sorted.bam +NORMAL_BAM_FILE=/n/scratch/users/${USER:0:1}/${USER}/variant_calling/alignments/${NORMAL_SAMPLE_NAME}_GRCh38.p7.coordinate_sorted.bam TUMOR_SAMPLE_NAME=syn3_tumor -TUMOR_BAM_FILE=/n/scratch3/users/${USER:0:1}/${USER}/variant_calling/alignments/${TUMOR_SAMPLE_NAME}_GRCh38.p7.coordinate_sorted.bam -VCF_OUTPUT_FILE=/n/scratch3/users/${USER:0:1}/${USER}/variant_calling/vcf_files/mutect2_${NORMAL_SAMPLE_NAME}_${TUMOR_SAMPLE_NAME}_GRCh38.p7-raw.vcf +TUMOR_BAM_FILE=/n/scratch/users/${USER:0:1}/${USER}/variant_calling/alignments/${TUMOR_SAMPLE_NAME}_GRCh38.p7.coordinate_sorted.bam +VCF_OUTPUT_FILE=/n/scratch/users/${USER:0:1}/${USER}/variant_calling/vcf_files/mutect2_${NORMAL_SAMPLE_NAME}_${TUMOR_SAMPLE_NAME}_GRCh38.p7-raw.vcf ```
@@ -408,10 +408,10 @@ module load gatk/4.1.9.0 REFERENCE_SEQUENCE=/n/groups/hbctraining/variant_calling/reference/GRCh38.p7.fa REFERENCE_DICTIONARY=`echo ${REFERENCE_SEQUENCE%fa}dict` NORMAL_SAMPLE_NAME=syn3_normal -NORMAL_BAM_FILE=/n/scratch3/users/${USER:0:1}/${USER}/variant_calling/alignments/${NORMAL_SAMPLE_NAME}_GRCh38.p7.coordinate_sorted.bam +NORMAL_BAM_FILE=/n/scratch/users/${USER:0:1}/${USER}/variant_calling/alignments/${NORMAL_SAMPLE_NAME}_GRCh38.p7.coordinate_sorted.bam TUMOR_SAMPLE_NAME=syn3_tumor -TUMOR_BAM_FILE=/n/scratch3/users/${USER:0:1}/${USER}/variant_calling/alignments/${TUMOR_SAMPLE_NAME}_GRCh38.p7.coordinate_sorted.bam -VCF_OUTPUT_FILE=/n/scratch3/users/${USER:0:1}/${USER}/variant_calling/vcf_files/mutect2_${NORMAL_SAMPLE_NAME}_${TUMOR_SAMPLE_NAME}_GRCh38.p7-raw.vcf +TUMOR_BAM_FILE=/n/scratch/users/${USER:0:1}/${USER}/variant_calling/alignments/${TUMOR_SAMPLE_NAME}_GRCh38.p7.coordinate_sorted.bam +VCF_OUTPUT_FILE=/n/scratch/users/${USER:0:1}/${USER}/variant_calling/vcf_files/mutect2_${NORMAL_SAMPLE_NAME}_${TUMOR_SAMPLE_NAME}_GRCh38.p7-raw.vcf # Run MuTect2 gatk Mutect2 \