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Some issues and questions #7

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CizarAlmalak opened this issue May 25, 2016 · 0 comments
Open

Some issues and questions #7

CizarAlmalak opened this issue May 25, 2016 · 0 comments

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@CizarAlmalak
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Using FGS+ I encountered a few things im not clear about and also encountered some issues.

  1. For some reason if I use "test" in the output name it automatically goes to stdout or it won't run at all.
    example:
    FragGeneScanPlus/FGS+ -s mydata.faa -m 5000 -o test.out -w 1 -t complete -p 10
  2. I'm also not very clear on what the "complete genomic sequence" means. Do full length genes fall in this category or as short sequence reads?
  3. what exactly is the + and - part in the title. I thought first it might mean whether it is in the + or - strand but that didn't make sense since in the following example both are in the same frame and strand orientation from the same sequence yet both are predicted separately and with each a + and a - :

complete sequence:
screen shot 2016-05-25 at 13 43 25

alternative prediction 1 of the same sequence:
screen shot 2016-05-25 at 13 42 34

alternative prediction 2 of the same sequence:
screen shot 2016-05-25 at 13 42 20

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