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load_open_ephys_data.m
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load_open_ephys_data.m
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function [data, timestamps, info] = load_open_ephys_data(filename)
%
% [data, timestamps, info] = load_open_ephys_data(filename)
%
% Loads continuous, event, or spike data files into Matlab.
%
% Inputs:
%
% filename: path to file
%
%
% Outputs:
%
% data: either an array continuous samples (in microvolts),
% a matrix of spike waveforms (in microvolts),
% or an array of event channels (integers)
%
% timestamps: in seconds
%
% info: structure with header and other information
%
%
%
% DISCLAIMER:
%
% Both the Open Ephys data format and this m-file are works in progress.
% There's no guarantee that they will preserve the integrity of your
% data. They will both be updated rather frequently, so try to use the
% most recent version of this file, if possible.
%
%
%
% ------------------------------------------------------------------
%
% Copyright (C) 2014 Open Ephys
%
% ------------------------------------------------------------------
%
% This program is free software: you can redistribute it and/or modify
% it under the terms of the GNU General Public License as published by
% the Free Software Foundation, either version 3 of the License, or
% (at your option) any later version.
%
% This program is distributed in the hope that it will be useful,
% but WITHOUT ANY WARRANTY; without even the implied warranty of
% MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
% GNU General Public License for more details.
%
% <http://www.gnu.org/licenses/>.
%
filetype = filename(max(strfind(filename,'.'))+1:end); % parse filetype
fid = fopen(filename);
filesize = getfilesize(fid);
% constants
NUM_HEADER_BYTES = 1024;
SAMPLES_PER_RECORD = 1024;
RECORD_MARKER = [0 1 2 3 4 5 6 7 8 255]';
RECORD_MARKER_V0 = [0 0 0 0 0 0 0 0 0 255]';
% constants for pre-allocating matrices:
MAX_NUMBER_OF_SPIKES = 1e6;
MAX_NUMBER_OF_RECORDS = 1e6;
MAX_NUMBER_OF_CONTINUOUS_SAMPLES = 1e8;
MAX_NUMBER_OF_EVENTS = 1e6;
SPIKE_PREALLOC_INTERVAL = 1e6;
%-----------------------------------------------------------------------
%------------------------- EVENT DATA ----------------------------------
%-----------------------------------------------------------------------
if strcmp(filetype, 'events')
disp(['Loading events file...']);
index = 0;
hdr = fread(fid, NUM_HEADER_BYTES, 'char*1');
eval(char(hdr'));
info.header = header;
if (isfield(info.header, 'version'))
version = info.header.version;
else
version = 0.0;
end
% pre-allocate space for event data
data = zeros(MAX_NUMBER_OF_EVENTS, 1);
timestamps = zeros(MAX_NUMBER_OF_EVENTS, 1);
info.sampleNum = zeros(MAX_NUMBER_OF_EVENTS, 1);
info.nodeId = zeros(MAX_NUMBER_OF_EVENTS, 1);
info.eventType = zeros(MAX_NUMBER_OF_EVENTS, 1);
info.eventId = zeros(MAX_NUMBER_OF_EVENTS, 1);
if (version >= 0.2)
recordOffset = 15;
else
recordOffset = 13;
end
while ftell(fid) + recordOffset < filesize % at least one record remains
index = index + 1;
if (version >= 0.1)
timestamps(index) = fread(fid, 1, 'int64', 0, 'l');
else
timestamps(index) = fread(fid, 1, 'uint64', 0, 'l');
end
info.sampleNum(index) = fread(fid, 1, 'int16'); % implemented after 11/16/12
info.eventType(index) = fread(fid, 1, 'uint8');
info.nodeId(index) = fread(fid, 1, 'uint8');
info.eventId(index) = fread(fid, 1, 'uint8');
data(index) = fread(fid, 1, 'uint8'); % save event channel as 'data' (maybe not the best thing to do)
if version >= 0.2
info.recordingNumber(index) = fread(fid, 1, 'uint16');
end
end
% crop the arrays to the correct size
data = data(1:index);
timestamps = timestamps(1:index);
info.sampleNum = info.sampleNum(1:index);
info.nodeId = info.nodeId(1:index);
info.eventType = info.eventType(1:index);
info.eventId = info.eventId(1:index);
%-----------------------------------------------------------------------
%---------------------- CONTINUOUS DATA --------------------------------
%-----------------------------------------------------------------------
elseif strcmp(filetype, 'continuous')
disp(['Loading ' filename '...']);
index = 0;
hdr = fread(fid, NUM_HEADER_BYTES, 'char*1');
eval(char(hdr'));
info.header = header;
if (isfield(info.header, 'version'))
version = info.header.version;
else
version = 0.0;
end
% pre-allocate space for continuous data
data = zeros(MAX_NUMBER_OF_CONTINUOUS_SAMPLES, 1);
info.ts = zeros(1, MAX_NUMBER_OF_RECORDS);
info.nsamples = zeros(1, MAX_NUMBER_OF_RECORDS);
if version >= 0.2
info.recNum = zeros(1, MAX_NUMBER_OF_RECORDS);
end
current_sample = 0;
RECORD_SIZE = 10 + SAMPLES_PER_RECORD*2 + 10; % size of each continuous record in bytes
if version >= 0.2
RECORD_SIZE = RECORD_SIZE + 2; % include recNum
end
while ftell(fid) + RECORD_SIZE <= filesize % at least one record remains
go_back_to_start_of_loop = 0;
index = index + 1;
if (version >= 0.1)
timestamp = fread(fid, 1, 'int64', 0, 'l');
nsamples = fread(fid, 1, 'uint16',0,'l');
if version >= 0.2
recNum = fread(fid, 1, 'uint16');
end
else
timestamp = fread(fid, 1, 'uint64', 0, 'l');
nsamples = fread(fid, 1, 'int16',0,'l');
end
if nsamples ~= SAMPLES_PER_RECORD && version >= 0.1
disp([' Found corrupted record...searching for record marker.']);
% switch to searching for record markers
last_ten_bytes = zeros(size(RECORD_MARKER));
for bytenum = 1:RECORD_SIZE*5
byte = fread(fid, 1, 'uint8');
last_ten_bytes = circshift(last_ten_bytes,-1);
last_ten_bytes(10) = double(byte);
if last_ten_bytes(10) == RECORD_MARKER(end);
sq_err = sum((last_ten_bytes - RECORD_MARKER).^2);
if (sq_err == 0)
disp([' Found a record marker after ' int2str(bytenum) ' bytes!']);
go_back_to_start_of_loop = 1;
break; % from 'for' loop
end
end
end
% if we made it through the approximate length of 5 records without
% finding a marker, abandon ship.
if bytenum == RECORD_SIZE*5
disp(['Loading failed at block number ' int2str(index) '. Found ' ...
int2str(nsamples) ' samples.'])
break; % from 'while' loop
end
end
if ~go_back_to_start_of_loop
block = fread(fid, nsamples, 'int16', 0, 'b'); % read in data
fread(fid, 10, 'char*1'); % read in record marker and discard
data(current_sample+1:current_sample+nsamples) = block;
current_sample = current_sample + nsamples;
info.ts(index) = timestamp;
info.nsamples(index) = nsamples;
if version >= 0.2
info.recNum(index) = recNum;
end
end
end
% crop data to the correct size
data = data(1:current_sample);
info.ts = info.ts(1:index);
info.nsamples = info.nsamples(1:index);
if version >= 0.2
info.recNum = info.recNum(1:index);
end
% convert to microvolts
data = data.*info.header.bitVolts;
timestamps = nan(size(data));
current_sample = 0;
if version >= 0.1
for record = 1:length(info.ts)
ts_interp = info.ts(record):info.ts(record)+info.nsamples(record);
timestamps(current_sample+1:current_sample+info.nsamples(record)) = ts_interp(1:end-1);
current_sample = current_sample + info.nsamples(record);
end
else % v0.0; NOTE: the timestamps for the last record will not be interpolated
for record = 1:length(info.ts)-1
ts_interp = linspace(info.ts(record), info.ts(record+1), info.nsamples(record)+1);
timestamps(current_sample+1:current_sample+info.nsamples(record)) = ts_interp(1:end-1);
current_sample = current_sample + info.nsamples(record);
end
end
%-----------------------------------------------------------------------
%--------------------------- SPIKE DATA --------------------------------
%-----------------------------------------------------------------------
elseif strcmp(filetype, 'spikes')
disp(['Loading spikes file...']);
index = 0;
hdr = fread(fid, NUM_HEADER_BYTES, 'char*1');
eval(char(hdr'));
info.header = header;
if (isfield(info.header, 'version'))
version = info.header.version;
else
version = 0.0;
end
num_channels = info.header.num_channels;
num_samples = 40; % **NOT CURRENTLY WRITTEN TO HEADER**
% pre-allocate space for spike data
data = zeros(MAX_NUMBER_OF_SPIKES, num_samples, num_channels);
timestamps = zeros(MAX_NUMBER_OF_SPIKES, 1);
info.source = zeros(MAX_NUMBER_OF_SPIKES, 1);
info.gain = zeros(MAX_NUMBER_OF_SPIKES, num_channels);
info.thresh = zeros(MAX_NUMBER_OF_SPIKES, num_channels);
if (version >= 0.4)
info.sortedId = zeros(MAX_NUMBER_OF_SPIKES, num_channels);
end
if (version >= 0.2)
info.recNum = zeros(MAX_NUMBER_OF_SPIKES, 1);
end
current_spike = 0;
last_percent=0;
while ftell(fid) + 512 < filesize % at least one record remains
current_spike = current_spike + 1;
current_percent= round(100* ((ftell(fid) + 512) / filesize));
if current_percent >= last_percent+10
last_percent=current_percent;
fprintf(' %d%%',current_percent);
end;
idx = 0;
% read in event type (1 byte)
event_type = fread(fid, 1, 'uint8'); % always equal to 4; ignore
idx = idx + 1;
if (version == 0.3)
event_size = fread(fid, 1, 'uint32', 0, 'l');
idx = idx + 4;
ts = fread(fid, 1, 'int64', 0, 'l');
idx = idx + 8;
elseif (version >= 0.4)
timestamps(current_spike) = fread(fid, 1, 'int64', 0, 'l');
idx = idx + 8;
ts_software = fread(fid, 1, 'int64', 0, 'l');
idx = idx + 8;
end
if (version < 0.4)
if (version >= 0.1)
timestamps(current_spike) = fread(fid, 1, 'int64', 0, 'l');
else
timestamps(current_spike) = fread(fid, 1, 'uint64', 0, 'l');
end
idx = idx + 8;
end
info.source(current_spike) = fread(fid, 1, 'uint16', 0, 'l');
idx = idx + 2;
num_channels = fread(fid, 1, 'uint16', 0, 'l');
num_samples = fread(fid, 1, 'uint16', 0, 'l');
idx = idx + 4;
if num_samples < 1 || num_samples > 10000
disp(['Loading failed at block number ' int2str(current_spike) '. Found ' ...
int2str(num_samples) ' samples.'])
break;
end
if (version >= 0.4)
info.sortedId(current_spike) = fread(fid, 1, 'uint16', 0, 'l');
electrodeId = fread(fid, 1, 'uint16', 0, 'l');
channel = fread(fid, 1, 'uint16', 0, 'l');
color = fread(fid, 3, 'uint8', 0, 'l');
pcProj = fread(fid, 2, 'single');
sampleFreq = fread(fid, 1, 'uint16', 0, 'l');
idx = idx + 19;
end
waveforms = fread(fid, num_channels*num_samples, 'uint16', 0, 'l');
idx = idx + num_channels*num_samples*2;
wv = reshape(waveforms, num_samples, num_channels);
if (version < 0.4)
channel_gains = fread(fid, num_channels, 'uint16', 0, 'l');
idx = idx + num_channels * 2;
else
channel_gains = fread(fid, num_channels, 'single');
idx = idx + num_channels * 4;
end
info.gain(current_spike,:) = channel_gains;
channel_thresholds = fread(fid, num_channels, 'uint16', 0, 'l');
idx = idx + num_channels * 2;
info.thresh(current_spike,:) = channel_thresholds;
if version >= 0.2
info.recNum(current_spike) = fread(fid, 1, 'uint16', 0, 'l');
idx = idx + 2;
end
data(current_spike, :, :) = wv;
end
fprintf('\n')
for ch = 1:num_channels % scale the waveforms
data(:, :, ch) = double(data(:, :, ch)-32768)./(channel_gains(ch)/1000);
end;
data = data(1:current_spike,:,:);
timestamps = timestamps(1:current_spike);
info.source = info.source(1:current_spike);
info.gain = info.gain(1:current_spike);
info.thresh = info.thresh(1:current_spike);
if version >= 0.2
info.recNum = info.recNum(1:current_spike);
end
if version >= 0.4
info.sortedId = info.sortedId(1:current_spike);
end
else
error('File extension not recognized. Please use a ''.continuous'', ''.spikes'', or ''.events'' file.');
end
fclose(fid); % close the file
if (isfield(info.header,'sampleRate'))
if ~ischar(info.header.sampleRate)
timestamps = timestamps./info.header.sampleRate; % convert to seconds
end
end
end
function filesize = getfilesize(fid)
fseek(fid,0,'eof');
filesize = ftell(fid);
fseek(fid,0,'bof');
end