diff --git a/tools/emboss_5/emboss_needle.xml b/tools/emboss_5/emboss_needle.xml index 14cf161a6f8..8bfddd3aca6 100644 --- a/tools/emboss_5/emboss_needle.xml +++ b/tools/emboss_5/emboss_needle.xml @@ -1,51 +1,72 @@ - + Needleman-Wunsch global alignment - macros.xml - + + - needle -asequence '$input1' -bsequence '$input2' -outfile '$out_file1' -gapopen $gapopen -gapextend $gapextend -brief $brief -aformat3 $out_format1 -auto + needle -version + - - - - - - - - - - - - - - - - - - - - - - + + + + + + + + + - + + + - - + + - + + + + + + + + + + + + + + + + - + + ]]> diff --git a/tools/emboss_5/emboss_needleall.xml b/tools/emboss_5/emboss_needleall.xml new file mode 100644 index 00000000000..cf1532e949c --- /dev/null +++ b/tools/emboss_5/emboss_needleall.xml @@ -0,0 +1,103 @@ + + Many-to-many Needleman-Wunsch global alignment + + macros.xml + + + + + needleall -version + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff --git a/tools/emboss_5/macros.xml b/tools/emboss_5/macros.xml index 57b78495d06..3484aec4437 100644 --- a/tools/emboss_5/macros.xml +++ b/tools/emboss_5/macros.xml @@ -1,11 +1,20 @@ + 23.1 5.0.0 + 6.6.0 emboss perl + + + emboss + perl + + + @@ -47,4 +56,142 @@ .*[^\\]$ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff --git a/tools/emboss_5/test-data/emboss_needle_out.fasta b/tools/emboss_5/test-data/emboss_needle_out.fasta new file mode 100644 index 00000000000..0cd969a77d9 --- /dev/null +++ b/tools/emboss_5/test-data/emboss_needle_out.fasta @@ -0,0 +1,676 @@ +>Sequence 561 BP; 135 A; 106 C; 98 G; 222 T; 0 other; +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +--------------------------------------------------------gtt- +-c----------gatgccta-aaataccttcttttg-----tcccta-------cacaga +-------------------------ccac------------------------------- +-------------------agt-------------------------------------- +--tttc-c--------------------------taatgg-ct-----ttacaccgacta +gaaattct-----------------------------------------------tgtgc +aa-gcac---------------------taattga--aagc------------------- +-----------ggt--tgg-------------c-ctagagtgttac--------cggttt +gtat---------------------------agc--------------tgagc-----gc +-----------------gtctcttgcc--ctgatcaaaggtt-cattttctctactttgg +aagacgttgt--------ggaa-----gaatacaacaagtacgagtc-----tctcc--- +-cc--------cctgg--tttgctgat----------------tactgg-----atac-- +-agttgtaatac-----ccttc-----------gcaac---------------------- +-ac----------cgcgtaact------atctatatg----------------------a +att------attttccct-------------ttatt--------atatgt---------- +----agtaggt-------------tcgtctttaatctt---------------------- +-cc----------------------ttta---gcaag-----------------tctttt +--------------------------------------------------------actg +tt----ttc--------------------------gacc--------tcaatgttca--- +------tgttc-----tt-----------------------------aggttg------t +ttt---------------------------ggataat--atgcggt-------------- +------cagttt----aatcttcgttgtt-tc----ttc------tta---------aaa +t----attt------att----catg-----------------gtttaatttt------t +ggtt-----------------------------tgtact----tgt---------tcagg +ggcc----------agttc---------------------------attat-----ttac +tctgtttgtat--ac----agcagtt-----------ctttt---atttttagtatg--- +---at---tttaat---ttaaaacaatt-----------cta---atg---gtca----- +-----------aaaa--------------------------------------------- +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +--------- +>hg17 +gtttgccatcttttgctgctctagggaatccagcagctgtcaccatgtaaacaagcccag +gctagaccaGTTACCCTCATCATCTTAGCTGATAGCCAGCCAGCCACCACAGGCAtgagt +caggccatattgctggacccacagaattatgagctaaataaatagtcttgggttaagcca +ctaagttttaggcatagtgtgttatgtaTCTCACAAACATATAAGACTGTGTGTTTGTTG +ACTGGAGGAAGAGATG-CTATAAAGACCACCTTTTAAAACTTCCCAAATACTGCCACTGA +TGTCCTGATGGAGGTATGAAAACATCCACTAAAATTTGTGGTTTATTCATTTTTCATTAT +TTTGTTTAAGGAGGTCTATAGTGGAAGAGGGAGATATTTGGggaaattttgtatagacta +gctttcacgatgttagggaattattattgtgtgataatggtcttgcagttacac-----a +gaaattcttccttattttttgggaagcaccaaagtagggataaaatgtcatgatgtgtgc +aatacactttaaaatgtttttgccaaaataattaatgaagcaaatatggaaaataataat +tattaaatctaggtgatgggtatattgtagttcactatagtattgcacacttttctgtat +gtttaaatttttcatttaaaaaaaaactttgagctagacaccaggctatgagctaggagc +atagcaatgaccaaatagactcctaccaac---tcaaagaatgcacattctC-----TGG +GAAAC-ATGTTTCCATTAGGAAGCCTCGAATGCAA----T--GTGACTGTGGTCTCCAGG +ACCTGTGTGATCCTGGCTTTTCCTGTTCCCTCCGCATCATCACTGCAGGTGTGTTTTCCC +AAGTTTTAA-ACATTTACCTTCCCAGTGGCCTTGCGTCTAGAGGAATCCCTGTATAGTGG +TACATGAATATAACACATAACAAAAATCATCTCTATGGTGTGTGTTGTTCCTGGGGTTCA +attcagcaaattttccctgggcacccatgtgttcttggcactggaaaagtaccgggactg +aaacagttgatggcccaatccctgtcctcttaaaacctaagggaggagaTGGAAAGGGGC +ACCCAACCCAGACTGAGAGACAGGAATTAGCTGCAAGGGGAACTAGGAAAAGCTTCTTTA +AGGATGGAGAGGCCCTAGTGGAATGGGGAGATTCTTCCGGGAGAAGCGATGGATGCACAG +TTGGGCATCCCCACAGACGGACTGGAAAGAAAAAAGGCCTGGAGGAATCAATGTGCAATG +TATGTGTGTTCCCTGGTTcaagggctgggaactttctctaaagggccaggtagaaaacat +tttaggctttctaagccaaggcaaaattgaggatattacatg-ggtacttatacaacaag +aataaacaatttacacaatttt--ttgttgacagaattcaaaactttatagacacagaaa +tgcaaatttcctgtaattttcccatgagaactattcttcttttgttttgttttgcgacAG +GGTTGCGCtgatcctcccgcctcagtctccctaagtgctgagatgttgcaggaagtcagg +gaccccgaacagagagatcggctggagccgtggcagaggaacataaattttgaagatttc +a-ttttaatatggacacttatcagttcccaaataatacttttataattttt--tatgcct +gtctttgctttaatctcttaatcctgttatcttcataagctaaggatgtacgtcacctca +ggaccactgtgataattgtgttaactgtacagattgattgcaaaacatgtgtgtttgaac +aatatgaaatcagtgcaccttgaaaaagagcagaataacagcaatttttagggaacaagg +gaagacaactataaggtctgactgcctgcggggtcgggcaaagggagccatatttttctt +cttgcagagagcctataaatagacctgcaagtaggagagatattgctaatttcttttgct +agcatggaatattaatattaacaccctgggaaaggaatgcattcctggggggaggtctat +aaatggccgctctgggaatgtctatcctacgcaatggagataaggactgagatacgccct +ggtctcctgcagtaccctcaggcttactagggtggtgaaaaactccgccctggtaaattt +gtggtcagaccagttttctgctctcgaacactgttttctgttgtttaagatgtttatcaa +gacaatacgtgcaccgctgaacacagacccttatcagtagttctcctttttgccctttga +agcatgtgatctactccctgttttacaccccctcaccttttgaaacccttaataaaaaac +ttgctggtttgaggctcaggtgggcatcacagtactaccgatatgtgatgtcacccccgg +cggcccagctgtaaaattcctctctttgtactctctctctttatttctcagccagctgac +acttatggaaaatagaaagaacctacgttgaaatattgggggcaggttcccccaataTCT +GGTGCCCAACGTGGGAtactgagattacaagcatgagccactgcatctggcctcttcttt +tgatttttttttttcaaacttttacaaatgtagaaaccattcttagcttttgggcattac +caaacccggcagtggcaggctcggttcaccaacgtcatttgcagttccccgCTTTATGTT +ATGGgttttgttttgttttgttttttttattgagacagagtttcactcttgttgcccagg +ctgtagtgcaatggtctgatcttggctcactgcaacctccacttcccaggttcaagccat +tctcctgcctcagcctctcaagtagctgggattacagacactcaccaccacacctggcta +attttgtatttttagtagagatgaggtttcaccatgttggccaggctggtctcgaaatcc +tgacctcaggtgatccacccaccttggcctcccaaagtgctgggattacaggcttgagct +accacgcctggctGGGTTGGTTCTCAATGGAGTGGTTTGTTTTTGGAGCTGCTCTGCGCA +Gtggggaccagaataggcctgggttcctagcccattgctattccttaccagctgtggatt +ctaaggaaagtcatttaacctcgctggaccttagattcctcatccctgaaGCCCAAGGGT +aaaacaaaacaaaacaaaacaaaacaaaccaaCCCATCATGTAAAGCGGGGAACTACAAA +CGATACAGGTGAAACATGCCTACCACACCACTCACAGGCTATGATGACAAAAACGTGGCT +ACATCTGGGACCACCCCCCAACCCCCACTTTGTACGTAGGAAATACGGAGTTGAGGATGG +AGACCCACAGTATGTCCAGAGTGTCCCCAAAGGCCACAGTGCCCGCCTGGAGCCCTCCAG +AGAGCGTGCACTCCCTGGGGTGCCAGCCAGAGACAACTTGCCCTGAGGCTTGGAACTCGA +TTCTCCGCGTGCCAGAGAAGGGGTGGGACTTCAGAACCCCCAACCCCGCAATCTGGGTCG +GGGAGCCTGGCGCACTGCGGGCCGCTCCCTCTAACCCTGGGCTTCCCTGGCGTCCAGGGC +CGTCGGGGCCGAGTCCCGATTCGCTCCCACCCCGAAGCCGCGCCAGGACCAACGAGGGCG +CAGCCGTATGCCCCAGCCCGCTCCGCGGAGCCCCTCACAGCCAcccccgccccgaccgcg +ccccgcgcggcTCGAAGCACCTTCCCAAGGGGCTGGTCCTTGCGCCATAGTCGCGCCGGA +GCCTCTGGAGGGACATCAAGGATTTCTCGCTCCTACCAGCCACCCCCAAATTTTTGGGAG +GTACCCAAGGGTGCGCGCGTGGCTCCTGGCGCGCCGAGGCCCTCCCTCGAGGCCCCGCGA +GGTGCACACTGCGGGCCCAGGGCTAGCAGCCGCCCGGCACGTCGCTACCCTGAGGGGCGG +GGCGGGAGCTGGCGCTAGAAATGCGCCGGGGCCTGCGGGGCAGTTGCGCAAGTTGTGATC +GGGCCGCTATAAGAGGGGCGGGCAGGCATGGAGCCCCGTAGGAATCGCAGCGCCAGCGGT +TGCAAGGTAAGGCCCCGGCGCGCTCCTTCCTCCTTCTCTGCTGGTCTTTCTTGGCAGGCC +ACAGGGCCCCACACAACTCTGGATCCCGGGGAAACTGAGTCAGGAGGGATGCAGGGCGGA +TGGCTTAGTTCTGGACTATGATAGCTTTGTACCGAGTTCTAGCCAGATAGAAGGTTACCG +GGAGCTGGGGAGCGTTGGATTTGCTGCTGGGCTGTGCCGGTGCCCAGAAGGCAGGACCTT +GCAGAACCAGCCAGGTCCCTGGGAGACTGTCAGACCCACCAACCTGGTGGCATTCGCAGA +GCTGAGATGCATTGGAAATTGCCTTGGGCACATCCCCAAAGATCAGGATGTCCCACCCCA +GTCTGAAGGAGATAAAGTTGGGGGTAGGAGAGACGCAGATGCAAGTGATCAGTCTCAGTC +CCAGACATTGCCTTGCTCTGCGGGTAGGAATTCAGGATTCATTTTCCAGGGAAGTTCCTG +ACCTCTGAATGAGAGGGGCTGTGTAAGGCCAATGCCTGGGAGGAAGGCAAGGATGAGTAG +AGGTGGGGGGAAACAAGTGTCAGGAAGACTCAAAATCTTCCAGAGAAATTGTGCAGGGTC +TTACCAGATCTGTCCTCAAAGCCATGCAAATTGCCTTCTTTGCAATGCATACAATGAGGT +GTCTCTGGGGGTCAGAACTGGTTATTAGGGAACTTCTAGCCAGGACTGCTAAATACGCGC +TGTTGGCCCACCAGGCTCACCTATAGCCTTCCTTCAGTCTGGGCTTGGTTTGGATTTCAC +TGTGGGTGCCATCGCCTTTACACTCCTGTTTCTATAGTTTAAAGATAGTGGTGCTTTGGG +AAAGTGACTCCTTAAATACAGTTAGGTCCAAGTGAGACAAGTGGCCTGGCTGTCATTTCA +GAATAGCAGCTTCCAAGAGGTGATTAATTTCTGTTGGAAGGGTGATCTTTGGGGAGGTGG +GTGAAGAGCAGAGACTTGGTGGTACCGTTCCAGGAGCACAGGCTCTCTTCCTTTGCAGTG +CAGAATGACCTCTGGCAGCCGGAGTTGTGTTTGTTCTGTAGGATTCTGAGGTGGGCCATG +GGCAGCTGGAACTGGGGAATTTTGCCAATCTCTTTCATATTAGGATTGTCTGCAGAACCA +GATATGGAGGCTTCTAGCAACGTGAGTGCTCCTGTTCTAATGCCCTTAGAAACAAGAAGG +CCACACTGATCATTTCTCTCACTTAGGCAGGGAGACAAGGCAAGAGAGAAACAGTGGATG +CTTTTAGGTTCTTTCCCTTCCCAAGCAGTTGTGGACATTGGGCTGAGGGGAACATTTCCA +CATTGGCTAAAGGAGCGTCCTCCTCATATTTTGTACATTTTATACCCAAAATAACTCTTC +TTGGTATTTGGGGAAATATTTTCCTCCCCGTCCATTCCAGGAAATGGCTCCAAGTGCCAA +GGACAGAGCCAGGGAAGTTGCAATGAATTCCTGCCCGTCAGCCCCAGGCAGATGCCTTGC +ACGTCTGAGTGGCCCATGCAGAGCGTGGAGGTGGCCGCCACGGAACCTGGGTCAATGTCC +CACCCCCGCTTAGATGCCACCAGGGGCGTGGGAGCCAAGGAGAGAAGAGGGGCTCCAGGA +AGGTAGAGTCCTTGTGTCTTGTGCATCTGTGAACAGCACTGGTATGATTTAAAGGAAAAT +TGAGCCAAATTTTCCGGCAGTCAGTTACCCCATCCCCACCGGGGTAGGAGTCTGGCAGCC +GCAGCTCCATTCTGGCCAGTCGGCAGAGAGCCTTGAAATTCTTCTTTGTCCACACAGTTG +TCTCAGAGAAACAGAGAGGTTGTTTCTGCTTAAAAACAACACACTTGGTGTCTGGGCCCA +CAGACTCCTTTGCACTTATTCCACGTGTGACAGCCAATGTGCCTCGTTGCTTAGCAGACA +GCATGTTACCGTCTTTCCTGCTCAGTTTGTTAGCTCTATGGAATGGAATTTATAATCAAT +GCCCATACCAACATTTCACTAATATCATAGGAGATTTAGTCTCCATCTGGGTGTACATTA +CATTTGCTCTGGGGTGCTCCAGGCTGGGGGGTTGCCAAGGAAGAGAAGAGAAACCGCAGA +GAAGACGGGAGGGCAGGGCAGGGGTCTCTGAGAAGGGGAGGGGTCCCAGAGTGCAGGAGC +AGGAGCCAGGCTCATGAAAGGGGCCACGGGCGGGAGTATCCAGGGACGGCAGTCAAGATG +GAGCACAGCTTAGGAAGCTGAAGGGAATCCTGGCCCACCTGGGTGCTAGAGGGCACATAG +GAAGTGCAGGAAGCAGACCAAGGTCCCCAAGAGAGGGAGACCTGGACGCTGAAGCATTTT +CTGTCTTTATTAAGACAACTCCGTAAGAATTCCTGCTGGGCCAAAGTGAATTCTAGGATG +CGACTTTAAGATGGGAGCAAGCGAACCATTGAGGAGGCAGGTTACCCTAGTTAGCCAATG +CAGATCGAGAATGGGAAATCTTTCatttattcatgcaacagatatttaacgaagccctgc +cgtgttccaggcctgtgatagatgctggaacaggtacagagatAcaggtgtcattaattg +atcagggcaacctctccttctgagtcttgctggagcttcagatgcccctcacacagagct +cgagggagcctcaacaattgatcagaagtcaggcaccatggctcacgcatataatcccag +cactttgggaggccaaggcaggtggatcactggagcccaggagttccagatcagctgggg +caacatggcaaaaccccatctctattaaaaaaaaaaaaagtaactggatgtgatggtaca +cacctgtagtcccagctacttgggaggctgagaggtgggagaattgcttgagcccgggaa +gtcgggggtccagtgagccttgatcacaccactgcactccagcctgagtgacagagcaag +accctgacacacacacacacacacacacacacacacacacagattagagctgaaacagga +gtagaaacctatctgtatctctgATGAGATCAGATCTTTCTGATGAACAGAAAGAATGTA +ACCCCTGTACTCACACCCTCTCTGCTGGTTACATATGTTAACACGATTTCTCAAATGAGG +CTTTTGGTTGCAAATAAGAGAAAATCACTCACGCTGGCCCTGTGTTTTTCAAATTGTTTA +TTGTGATCAACATTTGAAAAAAGAGCCGAGACTCTCAAGAGTGCATTACCCACGGTAAGG +GTGAATTTTACTTCTTGACACTTATTTCTCTTACATGTATCTATCTGTCTCAAATGAAAA +ATATATTTAGAAAGTTGAAAGCTATCCAAGTGAGTATAAGAAAAGAGTATCTCACCCTGA +AGGCTAAGGACAGGGAGGGCCACCAGGCCTCACGAGGACCCAGGAACCACAAAGAAGGCT +AGGAAGGAGCACAGGCGGTGACCATACTCTGGCTCAGTGGCTATGTGGGCTCTGGTCTCT +CTCAGCTGTTCCATGCATATGAGGCCAAATGTGGCTACCCTAGAGCTTCTGAGCCCTCAA +CAGAGATGAACTGGACTCTCTGCAGCCCCACTCTAAATTCCTAAGAGAGAAGTTGATTGA +CCCAATCAGGGTCAGGAGAAGGAAGGGAGGAGGAAAGGGAGGAGAGAAGAGCCTCTTCGT +CTCTTGCCTACCACTGGCCAGGCAATTGTAGCCAAGGGGGCTGGAGTGTAAATGCAAACA +TAGCCATCAAGGGTtgtgtatgtgtgtgtgtgtgtctgtgtgtgtgtatgtgtgtCTCTT +GGGTAGGTTAGATCTCCCAGGAGGTCCCTACTAAACAGACTTAAGCCCGCAAAATTTTAG +CTCTCCAGCCTCACACACTCCACCCCTCTACCATATTGAATCTTCCCAAACCAACTATGG +CTTTCCCTAACTCCGGAGcttggcctggaatgccctgcttcccctctttcccctggggaa +cgcctgtccttcaggcctcagttcacacactgcctcccttgcaaagctctccTCCCATCC +CCGGAGTCCCTCTTCCCCTTTGTTCTTTGGGTTCTATGCTTCTTCCCTCATAACTCCCAC +CAGGTTGTGTTAAAATGAGTTGTTCAAGGTCCTGTCTGTTCCACTAGATTCTGAGCAACT +TGGAGAACGAAGATCCAAACTTCGCTGCCTTTATTTCCTCCTTTGTTCTTTTCTCATCCC +CAAGTCCCTTCCAACTTGGAGTTATgaagaaaggaaggaaggaagggtgggagggaagaa +CAGGAGGGGATCCCACAGGAGAATGTGTATAGGGAGAGGACTCAGACTAGCTAAAGCTTT +TCCCTCATAATTAATAGCAAATACCATGTTACCTGAATTTAATTCACAGTAGCATACAAA +AGACTCGCTTTGTTCTCCCCATTGATGTCATCAGAGGGCTGTGGGCAGGCCTAATCTTGG +CTCAGGAGGCCCTCCAGCCTGGATCTAAAGAGCAGCAGATGggccaggctcggtggctca +tgcctgtaatcccagcattttgggaggccgaggcgggtggatcacgaggtcaggagtttg +agaccagcctggccaagatggtgaagcctcgtctctactaaaaatacaaaaattagccag +gtgcggtggtgggcgcctgtatttccagctacccgggaggctgaggaggctgaggcagga +gaatcgcttgaacccgggaggcggaggttgcagtgagccgaggtcacgccactgcactct +agcctgggcaacagagcaagactccgtcaaaaaaaaaataaaaaaataaaaaaataaaaa +aaataaaGAGGAGCACACATCTCTGCCCATCCTAACTCCCACTTTGACATTGAGGTCCCC +AGGATGGAGGGTCTGCCTCCATCTGCCTTGTCCCCTGCAATGGTGGGAAGGTGATGGAGC +TCAAGTCTAGAGGCCACCAGCTTCTTAGGGAGGTAGGAGGTGGAGGGTGGGGTGCGGGCC +CTGCACACAACTGCCAAGTGAGGATGGGGGTGGGGTCCACCTGAGGATAAGTAACAGTGA +GGCTGGTGCAGAGGACCCAGGTGGAGGTAGACAGCAGAATTTGTGGTGGGGTGGATGGCA +CATTATATAAGCCTCTCTTGCTGCCCTGTTTACTGAGATTGTTTCAttatcttttttggc +ttttgtttttaagagatggggtcttgctgtgtcacacaggctggagtgcactgtgtgatc +atacctcactgcagcctcgacatcctgggctcaggcaaacctcccaccttggcctcccaa +gtagctgggaccacaagcgtttgccaccacactcagctatttttatttttatttttattt +tttttagagatggggtcttgctgtgtcgcccaggctggtcttgaactcctgggctcaagc +gatcctcctgccttggcctcccaaagccctgggattataggctgagccaccacacccagc +cACATTTCATCTGTGCAGCTCCAGGGGCTCCACATTCTACTCTTCTCATTTCTTCTCCAG +GGTACCCATGGCAAGGGATGAGGGTAGAAGATGGGGCAGCCAGGCCTTGATTAAAGGAGA +AGGAAGGCAGCCTGTGGAGAGGGCAGCCCAGGGAGTGCAGAGAGAAGTGGGCCATGAGGG +AGACAGCAGAGTGCAGGCTGCGTCCCAAATGAGCATACAGCCCACTGTGAGCCCACCATC +TTCCTAGAGACCCCTCTCCTCTCCAGGAGCTGCTTCAGTAGCACTCAGAGGAAAGAATGA +TGCTGTATCAACATTTCAGCAGCTCATCTTTTAACTCTAAGAAAATGGCAGCTCCTAAAT +GTTCAAAACTGCTTTGGAAACTTCTGGAGAGAGGTTTTGCAGCTCAGGCAGACAGCTGAT +CGCGGCCTTTCTTCCACCCCAACCCATGCTCTCCCCATGCTCTCCTGCCACAGCTGCAGC +GGGCCCCTGGGTCCTACATTTGCAGCCCTTTGTCTCTGAGCTCAGACTTCCAATTCCAAG +CGGCAGCTGGGCAGGCTCACCAGCATGTCCAGCCAGTACTAGGACATCAGCAGGAGCCCA +ACCACCTCTTTCCAAAATCTCTCCTCATGTCTCTCCTAGTTTCCATCTCCATCCTTCTAG +TCAGCCAGGCTGAAAACATTTGCTCCTCAGGGTGCAGAAGGGAAAGCTTTGCCTCCCTTC +CTGGTGCTCACTGCCCCTGCGATTCCAGCCCAAGCCCTCCCCGGCTCCTCACCCTGGTGT +CAGCTGGAAGCCACCATCTCCTAAACCCACCTGtgttcttccacctctgccagggctgcc +ctctcctccaccttcacaaactcaattcctacccattctcaggtcccttatcaaatgcca +tctcctccatgatgcctccctgattccccTGCTGGAaataatggtgataacagctaaggc +attggggttggctacgtgccaggcaaggagttggcactttacatgctttatctcatttca +gccacataacatcgacaggtggcattatgattcatatcatccccatctgatagccaggaa +aactgagtcccagagaggttagccactttcctagggccCTGTGCTCTGACTCAAGCATAG +CTCTGAGGAACTCTAGCATTCATCAGTTTAAGCACCATGACTTTCTTTGCTGAGTCACCC +AAGGCATTTCTTCATTTAAATGTTCTTCCTTGGCCAGGCGCAGTGGCTCAggcccaatgc +ggtggctcacgcctgtaatctcaacactttgggaggccgaggtgggcagataatctgagg +tcaggagttcaagaccagcctggccaacatggtgaaaccccatctctactaaaaatacaa +aaaaatgaggctgggcgtgatgactcacacctgtaatcccagcactttgggaggccaagg +caggtggattacatgaggtcaggagttcgagaccagcctggccaacatggtgaaatccta +tctctattaaaaatacaaaaaattagccaggcatggtggcaggcacctgtaatcccagct +acttgggaggctgaggcaggaaaatggcttgaacccgggaggtggaggttgcagtgagcc +aaggttgcaccattgcactccagcctgggcaaaaagagggaaacatcgtctaaaaaagaa +aaaaaaaaaattagccaggctgggtggtgcatgcccgtaattccagctactcaggaggat +gaagcaagagaattgcttgaacccaggaggcagagattacagtgagctgagatcacaaca +ctgcactccagcctaggtaaagaacaagactccatctcaaaaataaataaataaaaataa +aTGTTCTTCCTTGCAATGAAGTTAAATATGTAAATTCTCAAACCAGTTGCTTAAGGGCAC +AGTTTTGTTCTTTACCTATATTTTTAACAAATATTTTATGTAAGTAGTTGACAAAATCAA +ATACTGTGTACACTACCGAGGCTTCCCTGGGAAAGCCATCAGCCTCTGCCCCATCCCTTC +CCACTCCTGATTCCACTTTCCTGTGTTTCCATATCTTTTTCATGTCTGTTTCTGGCCCAC +AGTGGGCGATCAATACATGTTAGCCACCAACCATCAAACCTATATTGAGTAATTATGGTA +TGTCAGGCACTATGCTCAATGAAATTGTAttaggcttgtacaaaagtaattgtggttttt +aagagtaatggcaaaaacggcagttactttcgcaccaacTATTTGCTGCCTTGAATTATT +CCTCCTCTCCTCATCCCTAAACCCTGCTCCTCCCAGCCATTCTTCCTCCCCTTCTTGGGC +CATGGCCAGGCCCCACCCAGGTACTAAGACTCAGGTGAACCAAGGAAGACTTAATGCCCA +CTCTTTTCTGATGCCCATGTTGGCATGTGTTAAGtcggttagcattaagtttggctgcat +ttagcagagacccaaaagaacagtgccttttaaaaggcagaggttatgtctctcacacac +acccagcacaagtccaagaccagcatggcatctcagctccatcaacctcaggaaccgagc +tcctgcagctccctgccctgcagttgataaggtgaggtctttgtcctcctggttcaagat +ggtgctagaatgttggctaccatatctatagtccaggcatcagaatggagcaagggatga +aaaaggaagagatgaaggcacacgacaggttcctgagagctggcacaggacacttctgct +tatatttcactggccagaacttagtcacatggtcacacctagttgggagactctgagaag +taaagtatttattctagatggccatatccctacctaagacttggagttttctatgactgg +ggaagaacggaagacaagatattgggaaagactagcagcctctactaAAAGGGTGATCtg +tgttgatgtgcgtgtgtgtgtgatgtttgtatgagcatgtgtgttatgtgttgtgtgtTG +GTGGGGCAGATTCTTGCGAGCACTTTGGTCTCAGATGGACCTGCTACCAGTTCTCTCTGC +AGACCCCCATAGGTTTCTCCTAAACCTGGCCTCTCCTATTAGGCAGCCTTACTCAGCGGC +AGCTTCTCAGCTCCATGTTTTCAAGGAACCACAATTTATTTCCAGCATCCACTGAAGCAT +ATTATCAGTGGTGATAGAGGGGGCTTGTAAAACTGTTTTTCCACTTAGGTATTAGAGGGT +GGCCATTATTTGAGAGTGACTATGACCACAGTTAATCTGGTAATAAATTCTCTTGGGTAG +GAGGGGGAAAGGAAAGGATGCTTTAAGGAAGCATCTTGCCAGGAGACACAAAGCTAACAA +GAGTGGAGCCTGCAGCTGGAGCCGCAGAGCCTAATCACTACACCCGCCCATCTCTGCTAG +GGTTTCATGACTTCGTATCGGGGATTAGCAGTATTTAACTCTGTTGCACAAACATTTGGT +GTATTATTCAGGTAACAAGTAGCTAATAGAGGAAGTTTTACTTTTTTAAGACATAAATTT +GCCTTTTCCCAAATTACTTGGTACATAGTACTTTTCATGTTTGAAGTTGAGATGTGGGTA +CAATACCATAGCTTTATTCCAGAGCAGGGTATTTGTTTCCAAATGCCATGTTCCCAGCAG +CTGCCCTTGACTGGGAATTGGGGTGTGATTTGGGCTTTTCCTTAAATCCTTGAGGAGCTG +GAGGGGTGGGTGGCTCGCACTCCTGCTTTctggatctgaatcctgactctgtcatggacc +tgtttgactttgggcaagttgactcctattcctgagccccatatttttctcttctgtgaa +attcagattaaaaAAACATGGCTTTGATCAAACATTATAAATAATATATAGACAGACTGC +TTGTTTTTATTGTATTGCCAGAAATGAATCCTACTAATATTGCCATCTATGGACAGAAAA +TGTATTACCTGTCTTCATCAAGACCCAGACGAGGAAGAACACGAAAAGCGGAGATTAATT +TTACTGCCATCTCCAGAACCGTCATCCTAATATTTACTTACATTTTATTATTATTTCAGG +CTCATGCACATATACTTAGCATGGATCATTGGCCACAGACTCGCATACATTTAACTTTAT +TACCTTTTGCCTCATGTATCTCATTAAAATTTTGCTGCTTAATCAAGGATCTGCATATTA +TTTTAATTTTAGAATTCACAGTTCCAAGACTTTGAAAGTTTCAAGCGTTCTGGGTGaatg +tgttatgctctctcccgccaccatgtctttataccccctgatttctcagccactatggca +accactttctactcttagtagcccatatttagtccaatccccagctcaggagacacttct +tccagggagccccctgtgccttccagtagtatcttgtacctgccctttttgcaaagctct +ttcctcctggcttagaatggcccattgacctgtttgtttctcctattaaactgtaagcca +ctcgagggtagagagcatctgttgttcaccattgcatcctcggtgctgagcactgcgtct +gacatattatttagaaggtcagtaagtgctagtgggatTCAGGCTCCCAGTGGGTGGGAG +AGAAAGGACGTAAGGAAGCAAGTGGTAAAGGCCCTCACAGAGTATCAGCAGGCTGGTGTG +AGGGAGAAATGCAGAGGATGGGTGAGTAGCATAATCGCTAATGATAGGGTAATGATAGAG +CACATTTCACAACACCTTtaagccctttcacgtgcatcagataatttgatcctcataaaa +gcctagagatagatatattacagggatgaaggtggagtattttgtggttatgtgatatgt +ttaaaattatgcagtgagtaaatgactgggttcaaaccagaccttaaaagtctgttatct +ttccCTCGAGCATGCAATGAAGTCTACATCATCCCTACCATGTCCATTTGATCACACCCT +GGCCTCACAGCTCTGTGGTCTACAGGATACCTCATGGTGGTTTTATTGACCAGACAATAA +TCCTCTTTCTAAGGGGATGCATTTCATTAATACATATGTAGATCATGAATTGTCTTTGAC +TTTGAGGGGATGGTAGCCAGAGCAGAAAGCAAAGCTGATTTTCATCCCCGTCTGGTAATG +TGGTTGGTAATGTGAAGATGGGTGTATTCTGAGATACCGGCTCCTTGCAGTGTGTGGTTC +CTTCTGTTTTCAGGCCCAAGAAGCCCATCCTGGGAAGGAAAATGCATTGGGGAACCCTGT +GCGGATTCTTGTGGCTTTGGCCCTATCTTTTCTATGTCCAAGCTGTGCCCATCCAAAAAG +TCCAAGATGACACCAAAACCCTCATCAAGACAATTGTCACCAGGATCAATGACATTTCAC +ACACGGTAAGGAGAGTATGCGGGGACAAAGTAGAACTGCAGCCAGCCCAGCACTGGCTCC +TAGTGGCACTGGACCCAGATAGtccaagaaacatttattgaacgcctcctgaatgccagg +cacctactggaagctgagaaGGATTTGAAAGCACAGGGCTCCACTCTTTCTGGTTGTTTC +TTTTGGCCCCTCTGCCTGCTGAGATTCCAGGGGTTAGTGGTTCTAATTCTAAACCACTCC +AAGAACATTTGATTTTGCTACATGTTTCCATTTAAAAATCATAGGATTTGggctgggtgt +ggtggcttgtacctgtcatcccagcactttgggaggccaaagcaggaggatcattcgagc +ccaagagttcgagaccagcctgggcagcatagggagaccccatctctacaaaaataataa +aaaatgttagctgggcatggtggtgtgtacctgtggtcccagctaggggaggctgagatg +gaaggatcacctgagcctgggaggttgaggctgcagtgggccctgatcatgccaccgtgc +tccagcctgggtgacagagtgagaccttgtctcaaaataaataaataaataaataaaAGT +CATAGGATTTgatcaggcatgatgggtcacatctgtaagcccattgctttaggaggccaa +ggtaggaggatcagttgaggccaggagttcaagaccagcctgggcaacatggcaagacct +ctctctctaatttttaaaaaaataaaaaTTAAAGATAAGAAAAAAATCATAGGATTCTCA +TGAGGCCTCACGTGCTTATTTTCAACCTACCAAGGGGAAACCCAGGCCTCAGCGATTAGC +TGAGCCACATGCAGGCACAGCCACTGTCTCTTTCCTTCCTGTCCCCTCTGTCCCCACCTT +CTGCGCTCGCCTTCCTCCCTGACTTCACTTCCTTGAATCTTAGTGCCTACGACCAGAGGG +AGCTGTGAAGTTCCTTGTGTCCCATTGGCAGGAACAAGACCCCCAGAAGCATCTCCTCAG +GGCCTCTATCCCATCTCTAGATGTGCTTGTCATTAGGGttcttgtagttccagctgatct +ctggccctgccgctcaaagatacccaaaagagcgagtctaccctttttcacattcaaccc +tctactgatttgcaaatagcagtcagtgcccaccctggtcttttctctggggtccagcag +gcctagaccttcagccattttcctgatgaGGTCTGTAtttgaaattaggaagattaagtt +tgaatcttcacacttctgatgtctgtgagatcttcagcaagttccttactgtctttaagc +cttgttttcatcatctggataatggggatatcacacactattcacaaggttgttatgagg +cctaaattagctaaagcaATTGAATCCTCCTTACCCCCTGCATGGAGCTCTCTGGAGACT +TCCACGTCTCCTGGTCATTGTGGGTGTCTTATGGTAGTCTTGGGCAGTTAGGGAGAAGTT +AGGTGTCTGGAAGCAAAGATGGCTCAGAACTAGATAGAGTCTTGGGCATTTTATAGATAA +AAACTCTTGTCTCCtttaaaaataataaaaaaaaattaGCTGGGCATATTAGCCACTCAG +CAAGACTGCACGTGATAGATCCCGAGTGCCCCACCTTGGGTGGTGTAATACACAATATCA +CGGGAGCCCCGGGTAGTAACCACGGAGGTGTCAGCCTCAGTGCTGTGGGCAGATGGATGG +GGAGAGCCTCCCGGAACTGGAGTCACTGGAGCAGGGTTGGGGGGCCTCACTGAGGGTACG +GCCTTGATCTCTAAGGAGGAGGGACTGCCTGGAAAAGCTGACTGGGAGGGAGGACTCGGC +TGGGGGTAGAAGGGACTAGGGAAGGCTGGGGGTGGGGGTGCTTATGGAGGACCTCAGATG +CCTGGGGAACAGACTCCACTAAATAAAACATATGAAACCATGGCTGGTTCTTCAGCAGAG +GCCATGTAGAGAAAGGAATGACCTAGGAAAGTTGGCCTGGAAGTGGAGGGAAGGATGGTG +TGGGAAAAGCAGGAATCTCGGAGACCAGCTTAGAGGCTTGGCAGTCACCTGGGTGCAGGA +TACAAGGGCCTGAGCCAAAGTGGTGAGGGAGGGTGGAAGGAGGCAGCCCAGAGAATGACC +CTCCATGCCCACGGGGAAGGCAGAGGGCTCTGAGAGCGATTCCTCCCACATGCTGAGCAC +TTGTTCTCCCTCTTCCTCCTGCATAGCAGTCAGTCTCCTCCAAACAGAAAGTCACCGGTT +TGGACTTCATTCCTGGGCTCCACCCCATCCTGACCTTATCCAAGATGGACCAGACACTGG +CAGTCTACCAACAGATCCTCACCAGTATGCCTTCCAGAAACGTGATCCAAATATCCAACG +ACCTGGAGAACCTCCGGGATCTTCTTCACGTGCTGGCCTTCTCTAAGAGCTGCCACTTGC +CCTGGGCCAGTGGCCTGGAGACCTTGGACAGCCTGGGGGGTGTCCTGGAAGCTTCAGGCT +ACTCCACAGAGGTGGTGGCCCTGAGCAGGCTGCAGGGGTCTCTGCAGGACATGCTGTGGC +AGCTGGACCTCAGCCCTGGGTGCTGAGGCCTTGAAGGTCACTCTTCCTGCAAGGACTACG +TTAAGGGAAGGAACTCTGGCTTCCAGGTATCTCCAGGATTGAAGAGCATTGCATGGACAC +CCCTTATCCAGGACTCTGTCAATTTCCCTGACTCCTCTAAGCCACTCTTCCAAAGGCATA +AGACCCTAAGCCTCCTTTTGCTTGAAACCAAAGATATATACACAGGATCCTATTCTCACC +AGGAAGGGGGTCCACCCAGCAAAGAGTGGGCTGCATCTGGGATTCCCACCAAGGTCTTCA +GCCATCAACAAGAGTTGTCTTGTCCCCTCTTGACCCATCTCCCCCTCACTGAATGCCTCA +ATGTGACCAGGGGTGATTTCAGAGAGGGCAGAGGGGTAGGCAGAGCCTTTGGATGACCAG +AACAAGGTTCCCTCTGAGAATTCCAAGGAGTTCCATGAAGACCACATCCACACACGCAGG +AACTCCCAGCAACACAAGCTGGAAGCACATGTTTATTTATTCTGCATTTTATTCTGGATG +GATTTGAAGCAAAGCACCAGCTTCTCCAGGCTCTTTGGGGTCAGCCAGGGCCAGGGGTCT +CCCTGGAGTGCAGTTTCCAATCCCATAGATGGGTCTGGCTGAGCTGAACCCATTTTGAGT +GACTCGAGGGTTGGGTTCATCTGAGCAAGAGCTGGCAAAGGTGGCTCTCCAGTTAGTTCT +CTCGTAACTGGTTTCATTTCTACTGTGACTGATGTTACATCACAGTGTTTGCAATGGTGT +TGCCCTGAGTGGATCTCCAAGGACCAGGTTATTTTAAAAAGATTTGTTTTGTCAAGTGTC +ATATGTAGGTGTCTGCACCCAGGGGTGGGGAATGTTTGGGCAGAAGGGAGAAGGATCTAG +AATGTGTTTTCTGAATAACATTTGTGTGGTGGGTTCTTTGGAAGGAGTGAGATCATTTTC +TTATCTTCTGCAATTGCTTAGGATGTTTTTCATGAAAATAGCTCTTTCAGGGGGGTTGTG +AGGCCTGGCCAGGCACCCCCTGGAGAGAAGTTTCTGGCCCTGGCTGACCCCAAAGAGCCT +GGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAAAGGGCTGAAAGCCA +TTTGTTGGGGCAGTGGTAAGCTCTGGCTTTCTCCGACTGCTAGGGAGTGGTCTTTCCTAT +CATGGAGTGACGGTCCCACACTGGTGACTGCGATCTTCAGAGCAGGGGTCCTTGGTGTGA +CCCTCTGAATGGTCCAGGGTTGATCACACTCTGGGTTTATTACATGGCAGTGTTCCTATT +TGGGGCTTGCATGCCAAATTGTAGTTCTTGTCTGATTGGCTCACCCAAGCAAGGCCAAAA +TTACCAAAAATCTTGGGGGGTTTTTACTCCAGTGGTGAAGAAAACTCCTTTAGCAGGTGG +TCCTGAGACCTGACAAGCACTGCTAGGCGAGTGCCAGGACTCCCCAGGCCAGGCCACCAG +GATGGCCCTTCCCACTGGAGGTCACATTCAGGAAGATGAAAGAGGAGGTTTGGGGTCTGC +CACCATCCTGCTGCTGTGTTTTTGCTATCACACAGTGGGTGGTGGATCTGTCCAAGGAAA +CTTGAATCAAAGCAGTTAACTTTAAGactgagcacctgcttcatgctcagccctgactgg +tgctataggctggagaagctcacccaataaacattaagattgaggcctgccctcagggat +cttgcattcccagtggTCAAACCGCACTCACCCATGTGCCAAGGTGGGGTATTTACCACA +GCAGCTGAACAGCCAAATGCATGGTGCAGTTGACAGCAGGTGGGAAATGGTATGAGCTGA +GGGGGGCCGTGCCCAGGGGCCCACAGGGAACCCTGCTTGCACTTTGTAACATGTTTACTT +TTCagggcatcttagcttctattatagccacatccctttgaaacaagataactgagaatt +taaaaataagaaaataTGACCCCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCC +ATCCAGAATAAAACGCAGACCCCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCC +ATCCAGAATAAAACGCAGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAAATGACC +CCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCATGAC +CCCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAGAC +CCCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAGAT +GCTTTGCTTCAAATCCATCCAGAATAAAACGCAGACCCCAAAGAGCCTGGAGAAGCTGAT +GCTTTGCTTCAAATCCATCCAGAATAAAACGCAGACCCCAAAGAGCCTGGAGAAGCTGAT +GCTTTGCTTCAAATCCATCCAGAATAAAACGCAGACCCCAAAGAGCCTGGAGAAGCTGAT +GCTTTGCTTCAAATCCATCCAGAATAAAACGCAGATGCTTTGCTTCAAATCCATCCAGAA +TAAAACGCA diff --git a/tools/emboss_5/test-data/emboss_needle_out.score b/tools/emboss_5/test-data/emboss_needle_out.score index 825a2947c15..7554c5e4d69 100644 --- a/tools/emboss_5/test-data/emboss_needle_out.score +++ b/tools/emboss_5/test-data/emboss_needle_out.score @@ -1,4 +1,4 @@ -Sequence hg17 20190 (604.5) +Sequence hg17 20196 (604.5) #--------------------------------------- #--------------------------------------- diff --git a/tools/emboss_5/test-data/emboss_needleall_input1.fa b/tools/emboss_5/test-data/emboss_needleall_input1.fa new file mode 100644 index 00000000000..72c8f1ab79a --- /dev/null +++ b/tools/emboss_5/test-data/emboss_needleall_input1.fa @@ -0,0 +1,62 @@ +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT +>Illumina_DpnII_Gex_Adapters1_1 +GATCGTCGGACTGTAGAACTCTGAAC +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAGTCCGAC +>Illumina_DpnII_Gex_Adapters2_1 +CAAGCAGAAGACGGCATACGA +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG +>Illumina_DpnII_Gex_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTACAGTCCGACGATC +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTGTAGAACTCTGAAC +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAGTCCGACATG +>Illumina_NlaIII_Gex_Adapters2_1 +CAAGCAGAAGACGGCATACGANN +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG +>Illumina_NlaIII_Gex_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTACAGTCCGACATG +>Illumina_Small_RNA_RT_Primer +CAAGCAGAAGACGGCATACGA +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTACAGTCCGACGATC +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTCTTCTGCTTGT +>Illumina_Small_RNA_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTACAGTCCGACGATC diff --git a/tools/emboss_5/test-data/emboss_needleall_input2.fa b/tools/emboss_5/test-data/emboss_needleall_input2.fa new file mode 100644 index 00000000000..e42f51bda43 --- /dev/null +++ b/tools/emboss_5/test-data/emboss_needleall_input2.fa @@ -0,0 +1,100 @@ +@FC12044_91407_8_200_406_24 +GTTAGCTCCCACCTTAAGATGTTTA ++FC12044_91407_8_200_406_24 +SXXTXXXXXXXXXTTSUXSSXKTMQ +@FC12044_91407_8_200_720_610 +CTCTGTGGCACCCCATCCCTCACTT ++FC12044_91407_8_200_720_610 +OXXXXXXXXXXXXXXXXXTSXQTXU +@FC12044_91407_8_200_345_133 +GATTTTTTAACAATAAACGTACATA ++FC12044_91407_8_200_345_133 +OQTOOSFORTFFFIIOFFFFFFFFF +@FC12044_91407_8_200_106_131 +GTTGCCCAGGCTCGTCTTGAACTCC ++FC12044_91407_8_200_106_131 +XXXXXXXXXXXXXXSXXXXISTXQS +@FC12044_91407_8_200_916_471 +TGATTGAAGGTAGGGTAGCATACTG ++FC12044_91407_8_200_916_471 +XXXXXXXXXXXXXXXUXXUSXXTXW +@FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAGGAAACCTG ++FC12044_91407_8_200_57_85 +XFXMXSXXSXXXOSQROOSROFQIQ +@FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG ++FC12044_91407_8_200_10_437 +USXSXXXXXXUXXXSXQXXUQXXKS +@FC12044_91407_8_200_154_436 +AGACCTTTGGATACAATGAACGACT ++FC12044_91407_8_200_154_436 +MKKMQTSRXMSQTOMRFOOIFFFFF +@FC12044_91407_8_200_336_64 +AGGGAATTTTAGAGGAGGGCTGCCG ++FC12044_91407_8_200_336_64 +STQMOSXSXSQXQXXKXXXKFXFFK +@FC12044_91407_8_200_620_233 +TCTCCATGTTGGTCAGGCTGGTCTC ++FC12044_91407_8_200_620_233 +XXXXXXXXXXXXXXXXXXXXXSXSW +@FC12044_91407_8_200_902_349 +TGAACGTCGAGACGCAAGGCCCGCC ++FC12044_91407_8_200_902_349 +XMXSSXMXXSXQSXTSQXFKSKTOF +@FC12044_91407_8_200_40_618 +CTGTCCCCACGGCGGGGGGGCCTGG ++FC12044_91407_8_200_40_618 +TXXXXSXXXXXXXXXXXXXRKFOXS +@FC12044_91407_8_200_83_511 +GATGTACTCTTACACCCAGACTTTG ++FC12044_91407_8_200_83_511 +SOXXXXXUXXXXXXQKQKKROOQSU +@FC12044_91407_8_200_76_246 +TCAAGGGTGGATCTTGGCTCCCAGT ++FC12044_91407_8_200_76_246 +XTXTUXXXXXRXXXTXXSUXSRFXQ +@FC12044_91407_8_200_303_427 +TTGCGACAGAGTTTTGCTCTTGTCC ++FC12044_91407_8_200_303_427 +XXQROXXXXIXFQXXXOIQSSXUFF +@FC12044_91407_8_200_31_299 +TCTGCTCCAGCTCCAAGACGCCGCC ++FC12044_91407_8_200_31_299 +XRXTSXXXRXXSXQQOXQTSQSXKQ +@FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGGGAAAGGCG ++FC12044_91407_8_200_553_135 +XSQQXXXXXXXXXXSXXMFFQXTKU +@FC12044_91407_8_200_139_74 +CCTCCCAGGTTCAAGCGATTATCCT ++FC12044_91407_8_200_139_74 +RMXUSXTXXQXXQUXXXSQISISSO +@FC12044_91407_8_200_108_33 +GTCATGGCGGCCCGCGCGGGGAGCG ++FC12044_91407_8_200_108_33 +OOOSSXXSXXOMKMOFMKFOKFFFF +@FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAAAGAAGAGTCG ++FC12044_91407_8_200_980_965 +TOSSRXXXSSMSXMOMXIRXOXFFS +@FC12044_91407_8_200_981_857 +AACGAGGGGCGCGACTTGACCTTGG ++FC12044_91407_8_200_981_857 +RXMSSXXXXSXQXQXFSXQFQKMXS +@FC12044_91407_8_200_8_865 +TTTCCCACCCCAGGAAGCCTTGGAC ++FC12044_91407_8_200_8_865 +XXXFKOROMKOORMIMRIIKKORFF +@FC12044_91407_8_200_292_484 +TCAGCCTCCGTGCCCAGCCCACTCC ++FC12044_91407_8_200_292_484 +XQXOSXXXXXUXXXXIXXXXQTOXF +@FC12044_91407_8_200_675_16 +CTCGGGAGGCTGAGGCAGGGGGGTT ++FC12044_91407_8_200_675_16 +OXTXXXSXXQXXOXXKMXXMXOKQF +@FC12044_91407_8_200_285_136 +CCAAATCTTGAATTGTAGCTCCCCT ++FC12044_91407_8_200_285_136 +OSXOQXXXXXSXXUXXTXXXXTRMS diff --git a/tools/emboss_5/test-data/emboss_needleall_out.fasta b/tools/emboss_5/test-data/emboss_needleall_out.fasta new file mode 100644 index 00000000000..23321842088 --- /dev/null +++ b/tools/emboss_5/test-data/emboss_needleall_out.fasta @@ -0,0 +1,3232 @@ +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAG--AGCTCGTATGCCGTCTT---CTGCTTG +>FC12044_91407_8_200_406_24 +--------GTTAGCTC-----CCACCTTAAGATGTTTA +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTT---TCCCTACACGACGCTCTTCCGATCT--- +>FC12044_91407_8_200_406_24 +-----GTTAGCTCCC-AC--------CTTAAGATGTTTA +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACC--GAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_406_24 +---GTTA--GCTCCCACCTTAAGATGTTTA------------------------------ +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACG--AGCT-----CTTCCGATCT--- +>FC12044_91407_8_200_406_24 +-------------------GTTAGCTCCCACCTTAAGATGTTTA +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTT---TCCCTACACGACGCTCTTCCGATCT--- +>FC12044_91407_8_200_406_24 +-----GTTAGCTCCC-AC--------CTTAAGATGTTTA +>Illumina_Paired_End_DNA_Adapters1_1 +-----------------GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG +>FC12044_91407_8_200_406_24 +GTTAGCTCCCACCTTAAGAT----------GTTTA-------------- +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTT---TCCCTACACGACGCTCTTCCGATCT--- +>FC12044_91407_8_200_406_24 +-----GTTAGCTCCC-AC--------CTTAAGATGTTTA +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACC--GAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_406_24 +---GTTA--GCTCCCACCTTAAGATGTTTA------------------------------ +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGT---CTCGGCATTCCTGCTGAACCGCTCTTCCG +ATCT--- +>FC12044_91407_8_200_406_24 +--------------------------GTTAGCTC---------------CCAC-CTTAAG +ATGTTTA +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTT---TCCCTACACGACGCTCTTCCGATCT--- +>FC12044_91407_8_200_406_24 +-----GTTAGCTCCC-AC--------CTTAAGATGTTTA +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGT---CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT--- +>FC12044_91407_8_200_406_24 +--GTTAGCTC---------------CCAC-CTTAAGATGTTTA +>Illumina_DpnII_Gex_Adapters1_1 +GATCGTCGGACTGTAGAACTCTGAAC------------ +>FC12044_91407_8_200_406_24 +----GT-------TAG--CTCCCACCTTAAGATGTTTA +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAG-TCCGAC------------- +>FC12044_91407_8_200_406_24 +-----------GTT----AGCTCCCACCTTAAGATGTTTA +>Illumina_DpnII_Gex_Adapters2_1 +--------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_406_24 +GTTAGCTCCCACCTTAAGA-TGTTTA--- +>Illumina_DpnII_Gex_Adapters2_2 +------TCGTATGCCGTCTT---CTGCTTG +>FC12044_91407_8_200_406_24 +GTTAGCTC-----CCACCTTAAGATGTTTA +>Illumina_DpnII_Gex_PCR_Primer_1 +--------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_406_24 +GTTAGCTCCCACCTTAAGA-TGTTTA--- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGA-GTTCTACAGTCCGA +>FC12044_91407_8_200_406_24 +---GTTA--GCTCCCACC------TTAAGATGTT-TA-------- +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTACAG-TCCGAC-----GATC---- +>FC12044_91407_8_200_406_24 +-------------GTT----AGCTCCCACCTTAAGATGTTTA +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTG-TAGAACTCTGAAC------------ +>FC12044_91407_8_200_406_24 +-------GTTAG--CTCCCACCTTAAGATGTTTA +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAG-TCCGAC------ATG---- +>FC12044_91407_8_200_406_24 +-----------GTT----AGCTCCCACCTTAAGATGTTTA +>Illumina_NlaIII_Gex_Adapters2_1 +--------CAAGCAGAAGACGGCATACGANN +>FC12044_91407_8_200_406_24 +GTTAGCTCCCACCTTAAGA-TGTTTA----- +>Illumina_NlaIII_Gex_Adapters2_2 +------TCGTATGCCGTCTT---CTGCTTG +>FC12044_91407_8_200_406_24 +GTTAGCTC-----CCACCTTAAGATGTTTA +>Illumina_NlaIII_Gex_PCR_Primer_1 +--------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_406_24 +GTTAGCTCCCACCTTAAGA-TGTTTA--- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGA-GTTCTACAGTCCGA +>FC12044_91407_8_200_406_24 +---GTTA--GCTCCCACC------TTAAGATGTT-TA-------- +>Illumina_NlaIII_Gex_sequencing_primer +-------CCGACAGGTTCAGA-GTTCTACAGTCCGACATG +>FC12044_91407_8_200_406_24 +GTTAGCTCC--CACCTTAAGATGTT-TA------------ +>Illumina_Small_RNA_RT_Primer +--------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_406_24 +GTTAGCTCCCACCTTAAGA-TGTTTA--- +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTACAG-TCCGAC-----GATC---- +>FC12044_91407_8_200_406_24 +-------GTT----AGCTCCCACCTTAAGATGTTTA +>Illumina_Small_RNA_3p_Adapter +------TCGTATGCCGTCTTCTGCTTGT--- +>FC12044_91407_8_200_406_24 +GTTAGCTC-----CCACCTTAAG-ATGTTTA +>Illumina_Small_RNA_PCR_Primer_1 +--------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_406_24 +GTTAGCTCCCACCTTAAGA-TGTTTA--- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGA-GTTCTACAGTCCGA +>FC12044_91407_8_200_406_24 +---GTTA--GCTCCCACC------TTAAGATGTT-TA-------- +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTACAG-TCCGAC-----GATC---- +>FC12044_91407_8_200_406_24 +-------------GTT----AGCTCCCACCTTAAGATGTTTA +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGTATG-----CCGT------CTTCTGCTTG +>FC12044_91407_8_200_720_610 +-----------CTC-TGTGGCACCCCATCCCTCACTT------- +>Illumina_Genomic_DNA_Adapters1_2 +-------ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_720_610 +CTCTGTGGCACCCCATCCCT--------CACTT------- +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCT---ACACTCTTTCCCTACACGACGCTCTTCCGATC +T +>FC12044_91407_8_200_720_610 +---------------------CTCTGTGGCACCCCATCCCT--------CACTT------ +- +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGCTCT------TCCGATCT------- +>FC12044_91407_8_200_720_610 +----------------------CTCTGTGGCACCCCATCCCTCACTT +>Illumina_Genomic_DNA_sequencing_primer +-------ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_720_610 +CTCTGTGGCACCCCATCCCT--------CACTT------- +>Illumina_Paired_End_DNA_Adapters1_2 +-------ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_720_610 +CTCTGTGGCACCCCATCCCT--------CACTT------- +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCT---ACACTCTTTCCCTACACGACGCTCTTCCGATC +T +>FC12044_91407_8_200_720_610 +---------------------CTCTGTGGCACCCCATCCCT--------CACTT------ +- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTC---GGCATTCCTGCTGAACCGCTCTTCCG +ATCT +>FC12044_91407_8_200_720_610 +----------------------------CTCTGTGGCACCCCATC----CCTCACTT--- +---- +>Illumina_Paired_End_DNA_sequencing_primer_1 +-------ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_720_610 +CTCTGTGGCACCCCATCCCT--------CACTT------- +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTC---GGCATTCCTGCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_720_610 +----CTCTGTGGCACCCCATC----CCTCACTT------- +>Illumina_DpnII_Gex_Adapters1_1 +GATCGTCGGACTGTAGAACTCT---GAAC-------------- +>FC12044_91407_8_200_720_610 +------------------CTCTGTGGCACCCCATCCCTCACTT +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAGTCCGAC-------------- +>FC12044_91407_8_200_720_610 +--------------CT-CTGTGGCACCCCATCCCTCACTT +>Illumina_DpnII_Gex_Adapters2_1 +CAAGCAGAAGAC-----GGCATACGA---------- +>FC12044_91407_8_200_720_610 +-----------CTCTGTGGCACCCCATCCCTCACTT +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG------------------ +>FC12044_91407_8_200_720_610 +------------CTCTG--TGGCACCCCATCCCTCACTT +>Illumina_DpnII_Gex_PCR_Primer_1 +CAAGCAGAAGAC-----GGCATACGA---------- +>FC12044_91407_8_200_720_610 +-----------CTCTGTGGCACCCCATCCCTCACTT +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGT-----CCGA---------- +>FC12044_91407_8_200_720_610 +---------------------------------CT-CTGTGGCACCCCATCCCTCACTT +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTACAGT----CCGACGATC-------- +>FC12044_91407_8_200_720_610 +----------------CT-CTGTGGCACC--CCATCCCTCACTT +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTGTAGAACTCT---GAAC-------------- +>FC12044_91407_8_200_720_610 +-------------CTCTGTGGCACCCCATCCCTCACTT +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAGT-----CCGACATG-------- +>FC12044_91407_8_200_720_610 +--------------CT-CTGTGGCACCC--CATCCCTCACTT +>Illumina_NlaIII_Gex_Adapters2_1 +CAAGCAGAAGAC-----GGCATACGANN-------- +>FC12044_91407_8_200_720_610 +-----------CTCTGTGGCACCCCATCCCTCACTT +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG------------------ +>FC12044_91407_8_200_720_610 +------------CTCTG--TGGCACCCCATCCCTCACTT +>Illumina_NlaIII_Gex_PCR_Primer_1 +CAAGCAGAAGAC-----GGCATACGA---------- +>FC12044_91407_8_200_720_610 +-----------CTCTGTGGCACCCCATCCCTCACTT +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGT-----CCGA---------- +>FC12044_91407_8_200_720_610 +---------------------------------CT-CTGTGGCACCCCATCCCTCACTT +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTACAGT-----CCGACATG-------- +>FC12044_91407_8_200_720_610 +-----------------CT-CTGTGGCACCC--CATCCCTCACTT +>Illumina_Small_RNA_RT_Primer +CAAGCAGAAGAC-----GGCATACGA---------- +>FC12044_91407_8_200_720_610 +-----------CTCTGTGGCACCCCATCCCTCACTT +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTACAGT----CCGACGATC-------- +>FC12044_91407_8_200_720_610 +----------CT-CTGTGGCACC--CCATCCCTCACTT +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTCTTCTGCT-TGT------------------- +>FC12044_91407_8_200_720_610 +-----------------CTCTGTGGCACCCCATCCCTCACTT +>Illumina_Small_RNA_PCR_Primer_1 +CAAGCAGAAGAC-----GGCATACGA---------- +>FC12044_91407_8_200_720_610 +-----------CTCTGTGGCACCCCATCCCTCACTT +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGT-----CCGA---------- +>FC12044_91407_8_200_720_610 +---------------------------------CT-CTGTGGCACCCCATCCCTCACTT +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTACAGT----CCGACGATC-------- +>FC12044_91407_8_200_720_610 +----------------CT-CTGTGGCACC--CCATCCCTCACTT +>Illumina_Genomici_DNA_Adapters1_1 +GAT--------------CGGAAGAGCTCGTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_345_133 +GATTTTTTAACAATAAACGTA------CATA---------------- +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTTCCGATCT-------------------- +>FC12044_91407_8_200_345_133 +----------------------------GATTTTTTAACAATAAACGTACATA +>Illumina_Genomic_DNA_PCR_Primers1_1 +-----------AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTC +TTCCGATCT +>FC12044_91407_8_200_345_133 +GATTTTTTAACAAT-AAACG---------------TACA---------TA---------- +--------- +>Illumina_Genomic_DNA_PCR_Primers1_2 +----------CAAGCAGAAGACG-GCATACGAGCTCTTCCGATCT +>FC12044_91407_8_200_345_133 +GATTTTTTAACAA----TAAACGTACATA---------------- +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGACGCTCTTCCGATCT-------------------- +>FC12044_91407_8_200_345_133 +----------------------------GATTTTTTAACAATAAACGTACATA +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG---------------------- +>FC12044_91407_8_200_345_133 +-----------------------------GATTTTTTAACAATAAACGTACATA +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTTCCGATCT-------------------- +>FC12044_91407_8_200_345_133 +----------------------------GATTTTTTAACAATAAACGTACATA +>Illumina_Paired_End_DNA_PCR_Primers1_1 +-----------AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTC +TTCCGATCT +>FC12044_91407_8_200_345_133 +GATTTTTTAACAAT-AAACG---------------TACA---------TA---------- +--------- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +----------CAAGCAGAAGACG-GCATACGAGATCGGTCTCGGCATTCCTGCTGAACCG +CTCTTCCGATCT +>FC12044_91407_8_200_345_133 +GATTTTTTAACAA----TAAACGTACATA------------------------------- +------------ +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGACGCTCTTCCGATCT-------------------- +>FC12044_91407_8_200_345_133 +----------------------------GATTTTTTAACAATAAACGTACATA +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-------------------- +>FC12044_91407_8_200_345_133 +--------------------------------GATTTTTTAACAATAAACGTACATA +>Illumina_DpnII_Gex_Adapters1_1 +GATCGTCGGACTGTAGAACTCTGAAC------- +>FC12044_91407_8_200_345_133 +--------GATTTTTTAACAATAAACGTACATA +>Illumina_DpnII_Gex_Adapters1_2 +--------------ACAGGTTCAGAGTTCTACAGTCCGAC +>FC12044_91407_8_200_345_133 +GATTTTTTAACAATAAACGTACATA--------------- +>Illumina_DpnII_Gex_Adapters2_1 +----------CAAGCAGAAGACG-GCATACGA +>FC12044_91407_8_200_345_133 +GATTTTTTAACAA----TAAACGTACATA--- +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG-------------------- +>FC12044_91407_8_200_345_133 +----------------GATTTTTTAACAATAAACGTACATA +>Illumina_DpnII_Gex_PCR_Primer_1 +----------CAAGCAGAAGACG-GCATACGA +>FC12044_91407_8_200_345_133 +GATTTTTTAACAA----TAAACGTACATA--- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCT--ACAGTCCGA---------- +>FC12044_91407_8_200_345_133 +----------------------------GATTTTTTAACAAT---AAACGTACATA +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCT--ACAGTCCGACGATC--- +>FC12044_91407_8_200_345_133 +-----------GATTTTTTAACAAT-AAACGTACATA +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTGTAGAACTCTGAAC------- +>FC12044_91407_8_200_345_133 +---GATTTTTTAACAATAAACGTACATA +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCT--ACAGT---CCGACATG +>FC12044_91407_8_200_345_133 +---------GATTTTTTAACAATAAACGTACATA +>Illumina_NlaIII_Gex_Adapters2_1 +----------CAAGCAGAAGACG-GCATACGANN +>FC12044_91407_8_200_345_133 +GATTTTTTAACAA----TAAACGTACATA----- +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG-------------------- +>FC12044_91407_8_200_345_133 +----------------GATTTTTTAACAATAAACGTACATA +>Illumina_NlaIII_Gex_PCR_Primer_1 +----------CAAGCAGAAGACG-GCATACGA +>FC12044_91407_8_200_345_133 +GATTTTTTAACAA----TAAACGTACATA--- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCT--ACAGTCCGA---------- +>FC12044_91407_8_200_345_133 +----------------------------GATTTTTTAACAAT---AAACGTACATA +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCT--ACAGT---CCGACATG +>FC12044_91407_8_200_345_133 +------------GATTTTTTAACAATAAACGTACATA +>Illumina_Small_RNA_RT_Primer +----------CAAGCAGAAGACG-GCATACGA +>FC12044_91407_8_200_345_133 +GATTTTTTAACAA----TAAACGTACATA--- +>Illumina_Small_RNA_5p_Adapter +------------------GTTCAGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_345_133 +GATTTTTTAACAATAAACGTACATA------------------- +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTCTTCTGCTTGT------------------- +>FC12044_91407_8_200_345_133 +----------------GATTTTTTAACAATAAACGTACATA +>Illumina_Small_RNA_PCR_Primer_1 +----------CAAGCAGAAGACG-GCATACGA +>FC12044_91407_8_200_345_133 +GATTTTTTAACAA----TAAACGTACATA--- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCT--ACAGTCCGA---------- +>FC12044_91407_8_200_345_133 +----------------------------GATTTTTTAACAAT---AAACGTACATA +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCT--ACAGTCCGACGATC--- +>FC12044_91407_8_200_345_133 +-----------GATTTTTTAACAAT-AAACGTACATA +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGTATGC-------CGTCTT---CTGCTTG +>FC12044_91407_8_200_106_131 +--------------GT-TGCCCAGGCTCGTCTTGAACTCC--- +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTTC-CGATCT-- +>FC12044_91407_8_200_106_131 +-----GTTGCCC------AGGCTCGTCTTGAACTCC +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTC-CGATCT- +- +>FC12044_91407_8_200_106_131 +------------------------------GTTGCCC------AGGCTCGTCTTGAACTC +C +>Illumina_Genomic_DNA_PCR_Primers1_2 +-----CAAGCAGAAGACGGCATACGAGCTCTTC-CGATCT-- +>FC12044_91407_8_200_106_131 +GTTGCCCAG-----------------GCTCGTCTTGAACTCC +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGACGCTCTTC-CGATCT-- +>FC12044_91407_8_200_106_131 +-----GTTGCCC------AGGCTCGTCTTGAACTCC +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG---------------- +>FC12044_91407_8_200_106_131 +-------------GT----------TGCCCAGGCTCGTCTTGAACTCC +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTTC-CGATCT-- +>FC12044_91407_8_200_106_131 +-----GTTGCCC------AGGCTCGTCTTGAACTCC +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTC-CGATCT- +- +>FC12044_91407_8_200_106_131 +------------------------------GTTGCCC------AGGCTCGTCTTGAACTC +C +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGT---CTCGGCATTCCTGCTGAACCGCTCTTCCG +ATCT +>FC12044_91407_8_200_106_131 +--------------------------GTTGCCCAGGC--TCGTCTTGAAC------TCC- +---- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGACGCTCTTC-CGATCT-- +>FC12044_91407_8_200_106_131 +-----GTTGCCC------AGGCTCGTCTTGAACTCC +>Illumina_Paired_End_DNA_sequencing_primer_2 +------CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_106_131 +GTTGCCCAGGCTCG-------TCTTGAAC------TCC----- +>Illumina_DpnII_Gex_Adapters1_1 +---------GATCGTCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_106_131 +GTTGCCCAGGCTCGTC------TTGAACTCC---- +>Illumina_DpnII_Gex_Adapters1_2 +-----ACAGGTTCAGAGTTCTACAGTCCGAC +>FC12044_91407_8_200_106_131 +GTTGCCCAGGCTC---GTCTTGAACTCC--- +>Illumina_DpnII_Gex_Adapters2_1 +------CAAGCAGAAGACGGCATACGA----- +>FC12044_91407_8_200_106_131 +GTTGCCCAGGC-----TCGTCTT--GAACTCC +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGC-------CGTCTT---CTGCTTG +>FC12044_91407_8_200_106_131 +--GT-TGCCCAGGCTCGTCTTGAACTCC--- +>Illumina_DpnII_Gex_PCR_Primer_1 +------CAAGCAGAAGACGGCATACGA----- +>FC12044_91407_8_200_106_131 +GTTGCCCAGGC-----TCGTCTT--GAACTCC +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTT---CAGAGT--TCT---ACAGTCCGA +>FC12044_91407_8_200_106_131 +-----------------------GTTGCCCAGGCTCGTCTTGAAC--TCC-- +>Illumina_DpnII_Gex_sequencing_primer +-----CGACAGGTTCAGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_106_131 +GTTGCC--CAGGCTC---GTCTTGAACTCC------- +>Illumina_NlaIII_Gex_Adapters1_1 +-----------TCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_106_131 +GTTGCCCAGGCTCG---TCTTGAACTCC---- +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAGTC-CGACATG------ +>FC12044_91407_8_200_106_131 +-----------GTTGCCCAGGCTCGTCTTGAACTCC +>Illumina_NlaIII_Gex_Adapters2_1 +------CAAGCAGAAGACGGCATACGANN--- +>FC12044_91407_8_200_106_131 +GTTGCCCAGGC-----TCGTCTT--GAACTCC +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGC-------CGTCTT---CTGCTTG +>FC12044_91407_8_200_106_131 +--GT-TGCCCAGGCTCGTCTTGAACTCC--- +>Illumina_NlaIII_Gex_PCR_Primer_1 +------CAAGCAGAAGACGGCATACGA----- +>FC12044_91407_8_200_106_131 +GTTGCCCAGGC-----TCGTCTT--GAACTCC +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTT---CAGAGT--TCT---ACAGTCCGA +>FC12044_91407_8_200_106_131 +-----------------------GTTGCCCAGGCTCGTCTTGAAC--TCC-- +>Illumina_NlaIII_Gex_sequencing_primer +----CCGACAGGTTCAGAGTTCTACAGTCCGACATG +>FC12044_91407_8_200_106_131 +GTTGCC--CAGGCTC---GTCTTGAACTCC------ +>Illumina_Small_RNA_RT_Primer +------CAAGCAGAAGACGGCATACGA----- +>FC12044_91407_8_200_106_131 +GTTGCCCAGGC-----TCGTCTT--GAACTCC +>Illumina_Small_RNA_5p_Adapter +GTT---CAGAGT--TCT---ACAGTCCGACGATC +>FC12044_91407_8_200_106_131 +GTTGCCCAGGCTCGTCTTGAAC--TCC------- +>Illumina_Small_RNA_3p_Adapter +TCGTATGC-------CGTCTT---CTGCTTGT +>FC12044_91407_8_200_106_131 +--GT-TGCCCAGGCTCGTCTTGAACTCC---- +>Illumina_Small_RNA_PCR_Primer_1 +------CAAGCAGAAGACGGCATACGA----- +>FC12044_91407_8_200_106_131 +GTTGCCCAGGC-----TCGTCTT--GAACTCC +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTT---CAGAGT--TCT---ACAGTCCGA +>FC12044_91407_8_200_106_131 +-----------------------GTTGCCCAGGCTCGTCTTGAAC--TCC-- +>Illumina_Small_RNA_sequencing_primer +-----CGACAGGTTCAGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_106_131 +GTTGCC--CAGGCTC---GTCTTGAACTCC------- +>Illumina_Genomici_DNA_Adapters1_1 +-GATCGGAAGAGCTCGTATGCCGT---CTTCTGCTTG +>FC12044_91407_8_200_916_471 +TGAT-TGAAG-----GTAGG--GTAGCATACTG---- +>Illumina_Genomic_DNA_Adapters1_2 +-------------------ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_916_471 +TGATTGAAGGTAGGGTAGCATACTG--------------------------- +>Illumina_Genomic_DNA_PCR_Primers1_1 +------AATG---------ATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGAC +GCTCTTCCGATCT +>FC12044_91407_8_200_916_471 +TGATTGAAGGTAGGGTAGCATACTG----------------------------------- +------------- +>Illumina_Genomic_DNA_PCR_Primers1_2 +-----CAAGCAGAAGACG---GCATAC-GAGCTCTTCCGATCT +>FC12044_91407_8_200_916_471 +TGATTGAAG--GTAG--GGTAGCATACTG-------------- +>Illumina_Genomic_DNA_sequencing_primer +-------------------ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_916_471 +TGATTGAAGGTAGGGTAGCATACTG--------------------------- +>Illumina_Paired_End_DNA_Adapters1_1 +-GATCGGAAG--AGCGGTTCAGCAGGAATGCCGAG +>FC12044_91407_8_200_916_471 +TGAT-TGAAGGTAG-GGT--AGCA-TACTG----- +>Illumina_Paired_End_DNA_Adapters1_2 +-------------------ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_916_471 +TGATTGAAGGTAGGGTAGCATACTG--------------------------- +>Illumina_Paired_End_DNA_PCR_Primers1_1 +------AATG---------ATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGAC +GCTCTTCCGATCT +>FC12044_91407_8_200_916_471 +TGATTGAAGGTAGGGTAGCATACTG----------------------------------- +------------- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +-----CAAGCAGAAGACG---GCATAC-GAGATCGGTCTCGGCATTCCTGCTGAACCGCT +CTTCCGATCT +>FC12044_91407_8_200_916_471 +TGATTGAAG--GTAG--GGTAGCATACTG------------------------------- +---------- +>Illumina_Paired_End_DNA_sequencing_primer_1 +-------------------ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_916_471 +TGATTGAAGGTAGGGTAGCATACTG--------------------------- +>Illumina_Paired_End_DNA_sequencing_primer_2 +------------CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_916_471 +TGATTGAAGGTAGGGT---AGCA-TACTG-------------------- +>Illumina_DpnII_Gex_Adapters1_1 +-----GATCGTCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_916_471 +TGATTGAAGGTAGG---GTAGCATACTG--- +>Illumina_DpnII_Gex_Adapters1_2 +------ACAGGTTCAGAGT--TCTACAGTCCGAC +>FC12044_91407_8_200_916_471 +TGATTGA-AGGT--AGGGTAGCATACTG------ +>Illumina_DpnII_Gex_Adapters2_1 +-----CAAGCAGAAGACG---GCATAC-GA +>FC12044_91407_8_200_916_471 +TGATTGAAG--GTAG--GGTAGCATACTG- +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG------------------- +>FC12044_91407_8_200_916_471 +---------------TGATTGAAGGTAGGGTAGCATACTG +>Illumina_DpnII_Gex_PCR_Primer_1 +-----CAAGCAGAAGACG---GCATAC-GA +>FC12044_91407_8_200_916_471 +TGATTGAAG--GTAG--GGTAGCATACTG- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGT--TCTACAGTCCGA +>FC12044_91407_8_200_916_471 +--TGAT----TGA--------AGGT--AGGGTAGCATACTG----- +>Illumina_DpnII_Gex_sequencing_primer +----CGACAGGTTCAGAGT--TCTACAGTCCGACGATC +>FC12044_91407_8_200_916_471 +TGATTGA-AGGT--AGGGTAGCATACTG---------- +>Illumina_NlaIII_Gex_Adapters1_1 +----------TCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_916_471 +TGATTGAAGGTAGG---GTAGCATACTG--- +>Illumina_NlaIII_Gex_Adapters1_2 +------ACAGGTTCAGAGT--TCTACAGTCCGACATG +>FC12044_91407_8_200_916_471 +TGATTGA-AGGT--AGGGTAGCATACTG--------- +>Illumina_NlaIII_Gex_Adapters2_1 +-----CAAGCAGAAGACG---GCATAC-GANN +>FC12044_91407_8_200_916_471 +TGATTGAAG--GTAG--GGTAGCATACTG--- +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG------------------- +>FC12044_91407_8_200_916_471 +---------------TGATTGAAGGTAGGGTAGCATACTG +>Illumina_NlaIII_Gex_PCR_Primer_1 +-----CAAGCAGAAGACG---GCATAC-GA +>FC12044_91407_8_200_916_471 +TGATTGAAG--GTAG--GGTAGCATACTG- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGT--TCTACAGTCCGA +>FC12044_91407_8_200_916_471 +--TGAT----TGA--------AGGT--AGGGTAGCATACTG----- +>Illumina_NlaIII_Gex_sequencing_primer +---CCGACAGGTTCAGAGT--TCTACAGTCCGACATG +>FC12044_91407_8_200_916_471 +TGATTGA-AGGT--AGGGTAGCATACTG--------- +>Illumina_Small_RNA_RT_Primer +-----CAAGCAGAAGACG---GCATAC-GA +>FC12044_91407_8_200_916_471 +TGATTGAAG--GTAG--GGTAGCATACTG- +>Illumina_Small_RNA_5p_Adapter +---------GTTCAGAGT--TCTACAGTCCGACGATC +>FC12044_91407_8_200_916_471 +TGATTGAAGGT--AGGGTAGCATACTG---------- +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTCTTCTGCTTGT------------------ +>FC12044_91407_8_200_916_471 +---------------TGATTGAAGGTAGGGTAGCATACTG +>Illumina_Small_RNA_PCR_Primer_1 +-----CAAGCAGAAGACG---GCATAC-GA +>FC12044_91407_8_200_916_471 +TGATTGAAG--GTAG--GGTAGCATACTG- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGT--TCTACAGTCCGA +>FC12044_91407_8_200_916_471 +--TGAT----TGA--------AGGT--AGGGTAGCATACTG----- +>Illumina_Small_RNA_sequencing_primer +----CGACAGGTTCAGAGT--TCTACAGTCCGACGATC +>FC12044_91407_8_200_916_471 +TGATTGA-AGGT--AGGGTAGCATACTG---------- +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGTATGCCGTCTTCTG------CTTG +>FC12044_91407_8_200_57_85 +----------GCTCCAATAGCG----CAGAGGAAACCTG +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTC---TTCCG--------ATCT- +>FC12044_91407_8_200_57_85 +--------------------GCTCCAATAGCGCAGAGGAAACCTG +>Illumina_Genomic_DNA_PCR_Primers1_1 +-----AATGATACGGCGACCACCGAG---ATCTACACTCTTTCCCTACACGACGCTCTTC +CGATCT +>FC12044_91407_8_200_57_85 +GCTCCAAT---------AGCGCAGAGGAAACCTG-------------------------- +------ +>Illumina_Genomic_DNA_PCR_Primers1_2 +----CAA----GCAGAAGA---CGGCATACGAGCTCTTCCGATCT +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAGGAAACCTG-------------------- +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGACGCTC---TTCCG--------ATCT- +>FC12044_91407_8_200_57_85 +--------------------GCTCCAATAGCGCAGAGGAAACCTG +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAA---TGCCGAG +>FC12044_91407_8_200_57_85 +GCTC-CAATAGCG---CAG-AGGAAACCTG----- +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTC---TTCCG--------ATCT- +>FC12044_91407_8_200_57_85 +--------------------GCTCCAATAGCGCAGAGGAAACCTG +>Illumina_Paired_End_DNA_PCR_Primers1_1 +-----AATGATACGGCGACCACCGAG---ATCTACACTCTTTCCCTACACGACGCTCTTC +CGATCT +>FC12044_91407_8_200_57_85 +GCTCCAAT---------AGCGCAGAGGAAACCTG-------------------------- +------ +>Illumina_Paired_End_DNA_PCR_Primers1_2 +----CAA----GCAGAAGA---CGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCG +CTCTTCCGATCT +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAGGAAACCTG----------------------------------- +------------ +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGACGCTC---TTCCG--------ATCT- +>FC12044_91407_8_200_57_85 +--------------------GCTCCAATAGCGCAGAGGAAACCTG +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATT-------------CCTGCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_57_85 +---GCTC--CAATAGCGCAGAGGAAACCTG-------------------- +>Illumina_DpnII_Gex_Adapters1_1 +--------------GATCGTCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGA-----GGA------AAC-CTG--- +>Illumina_DpnII_Gex_Adapters1_2 +------ACAG-GTTCAGAGTTCTACAGTCCGAC----- +>FC12044_91407_8_200_57_85 +GCTCCAATAGCG--CAGAG-----------GAAACCTG +>Illumina_DpnII_Gex_Adapters2_1 +----CAA----GCAGAAGA---CGGCATACGA +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAGGAAACCTG------- +>Illumina_DpnII_Gex_Adapters2_2 +--TCGTATGCCGTCTTCTG------CTTG +>FC12044_91407_8_200_57_85 +GCTCCAATAGCG----CAGAGGAAACCTG +>Illumina_DpnII_Gex_PCR_Primer_1 +----CAA----GCAGAAGA---CGGCATACGA +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAGGAAACCTG------- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAG-GTTCAGAGTTCTACAGTCCGA------ +>FC12044_91407_8_200_57_85 +-------------GCTCCAATAGCG--CAGAG-----------GAAACCTG +>Illumina_DpnII_Gex_sequencing_primer +----------CGACAGGTTCAGAGTTCTACAGTCCGACGATC--- +>FC12044_91407_8_200_57_85 +GCTCCAATAGCG-------CAGAG-----------GA--AACCTG +>Illumina_NlaIII_Gex_Adapters1_1 +-------TCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAGGAAACCTG--- +>Illumina_NlaIII_Gex_Adapters1_2 +------ACAG-GTTCAGAGTTCTACAGTCCG--ACATG +>FC12044_91407_8_200_57_85 +GCTCCAATAGCG--CAGAG-----------GAAACCTG +>Illumina_NlaIII_Gex_Adapters2_1 +----CAA----GCAGAAGA---CGGCATACGANN +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAGGAAACCTG--------- +>Illumina_NlaIII_Gex_Adapters2_2 +--TCGTATGCCGTCTTCTG------CTTG +>FC12044_91407_8_200_57_85 +GCTCCAATAGCG----CAGAGGAAACCTG +>Illumina_NlaIII_Gex_PCR_Primer_1 +----CAA----GCAGAAGA---CGGCATACGA +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAGGAAACCTG------- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAG-GTTCAGAGTTCTACAGTCCGA------ +>FC12044_91407_8_200_57_85 +-------------GCTCCAATAGCG--CAGAG-----------GAAACCTG +>Illumina_NlaIII_Gex_sequencing_primer +---CCGACAG-GTTCAGAGTTCTACAGTCCG--ACATG +>FC12044_91407_8_200_57_85 +GCTCCAATAGCG--CAGAG-----------GAAACCTG +>Illumina_Small_RNA_RT_Primer +----CAA----GCAGAAGA---CGGCATACGA +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAGGAAACCTG------- +>Illumina_Small_RNA_5p_Adapter +---------GTTCAGAGTTCTACAGTCCGACGATC--- +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAG-----------GA--AACCTG +>Illumina_Small_RNA_3p_Adapter +--TCGTATGCCGTCTTCTG------CTTGT +>FC12044_91407_8_200_57_85 +GCTCCAATAGCG----CAGAGGAAACCTG- +>Illumina_Small_RNA_PCR_Primer_1 +----CAA----GCAGAAGA---CGGCATACGA +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAGGAAACCTG------- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAG-GTTCAGAGTTCTACAGTCCGA------ +>FC12044_91407_8_200_57_85 +-------------GCTCCAATAGCG--CAGAG-----------GAAACCTG +>Illumina_Small_RNA_sequencing_primer +----------CGACAGGTTCAGAGTTCTACAGTCCGACGATC--- +>FC12044_91407_8_200_57_85 +GCTCCAATAGCG-------CAGAG-----------GA--AACCTG +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG----------------- +>FC12044_91407_8_200_10_437 +-------------------------GCTGCTTGGGAGGCTGAGGCAGGAG +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT---------- +>FC12044_91407_8_200_10_437 +--------------------GCTGCTTGGGAGGCTGAGGCAGGAG +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT +---------- +>FC12044_91407_8_200_10_437 +---------------------------------------------GCTGCTTGGGAGGCT +GAGGCAGGAG +>Illumina_Genomic_DNA_PCR_Primers1_2 +---------------CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG------------------------ +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT---------- +>FC12044_91407_8_200_10_437 +--------------------GCTGCTTGGGAGGCTGAGGCAGGAG +>Illumina_Paired_End_DNA_Adapters1_1 +---GATCGGAAGAGCGGTTCA-GCAGGAATGCCGAG +>FC12044_91407_8_200_10_437 +GCTGCTTGG----GAGGCTGAGGCAGGAG------- +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT---------- +>FC12044_91407_8_200_10_437 +--------------------GCTGCTTGGGAGGCTGAGGCAGGAG +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT +---------- +>FC12044_91407_8_200_10_437 +---------------------------------------------GCTGCTTGGGAGGCT +GAGGCAGGAG +>Illumina_Paired_End_DNA_PCR_Primers1_2 +---------------CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGA +ACCGCTCTTCCGATCT +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG----------------------------------- +---------------- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT---------- +>FC12044_91407_8_200_10_437 +--------------------GCTGCTTGGGAGGCTGAGGCAGGAG +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGA-TCT---------- +>FC12044_91407_8_200_10_437 +----------------GCTG-------CTTGGGAGGCTGAGGCAGGAG +>Illumina_DpnII_Gex_Adapters1_1 +---------GATCGTCGGA--CTGTAGAACTCTGAAC +>FC12044_91407_8_200_10_437 +GCTGCTTGGGA--GGCTGAGGCAGGAG---------- +>Illumina_DpnII_Gex_Adapters1_2 +------------------ACAGGTTCAGAGTTCTACAGTCCGAC +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGG---AG---------------- +>Illumina_DpnII_Gex_Adapters2_1 +---------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG----------- +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG----------------- +>FC12044_91407_8_200_10_437 +-------------GCTGCTTGGGAGGCTGAGGCAGGAG +>Illumina_DpnII_Gex_PCR_Primer_1 +---------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG----------- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_10_437 +---GCTGCTTGGGAGGCTGA-----GGCAGG---AG--------------- +>Illumina_DpnII_Gex_sequencing_primer +----------------CGACAGGTTCAGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGG---AG-------------------- +>Illumina_NlaIII_Gex_Adapters1_1 +------TCGGA--------CTGTAGAACTCTGAAC +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG---------- +>Illumina_NlaIII_Gex_Adapters1_2 +------------------ACAGGTTCAGAGTTCTACAGTCCGACATG +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGG---AG------------------- +>Illumina_NlaIII_Gex_Adapters2_1 +---------------CAAGCAGAAGACGGCATACGANN +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG------------- +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG----------------- +>FC12044_91407_8_200_10_437 +-------------GCTGCTTGGGAGGCTGAGGCAGGAG +>Illumina_NlaIII_Gex_PCR_Primer_1 +---------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG----------- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_10_437 +---GCTGCTTGGGAGGCTGA-----GGCAGG---AG--------------- +>Illumina_NlaIII_Gex_sequencing_primer +-------------CCGA--CAGGTTCAGAGTTCTACAGTCCGACATG +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGG---AG------------------- +>Illumina_Small_RNA_RT_Primer +---------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG----------- +>Illumina_Small_RNA_5p_Adapter +------------GTTCA-----GAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG------------------ +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTCTTCTGCTTGT---------------- +>FC12044_91407_8_200_10_437 +-------------GCTGCTTGGGAGGCTGAGGCAGGAG +>Illumina_Small_RNA_PCR_Primer_1 +---------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG----------- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_10_437 +---GCTGCTTGGGAGGCTGA-----GGCAGG---AG--------------- +>Illumina_Small_RNA_sequencing_primer +----------------CGACAGGTTCAGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGG---AG-------------------- +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGC-TCGTATGC------CGTCTTCTGCTTG +>FC12044_91407_8_200_154_436 +-------AGACCTTTGGATACAATGAACGACT-------- +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTAC-----ACGACGCTCTTCCGATCT +>FC12044_91407_8_200_154_436 +AGAC-CTTTGGATACAATGAACGACT------------ +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTAC-----ACGACGCTCTTCCGA +TCT +>FC12044_91407_8_200_154_436 +-------------------------AGAC-CTTTGGATACAATGAACGACT--------- +--- +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGAC----GGCATAC---GAGCTCTTCCGATCT +>FC12044_91407_8_200_154_436 +--------AGACCTTTGG-ATACAATGA------ACGA-CT +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTAC-----ACGACGCTCTTCCGATCT +>FC12044_91407_8_200_154_436 +AGAC-CTTTGGATACAATGAACGACT------------ +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGC----GGTTCAGCAGGAATGCCGAG- +>FC12044_91407_8_200_154_436 +-------AGACCTTTGGATACA--ATGAA---CGACT +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTAC-----ACGACGCTCTTCCGATCT +>FC12044_91407_8_200_154_436 +AGAC-CTTTGGATACAATGAACGACT------------ +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTAC-----ACGACGCTCTTCCGA +TCT +>FC12044_91407_8_200_154_436 +-------------------------AGAC-CTTTGGATACAATGAACGACT--------- +--- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGAC----GGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCC +GATCT +>FC12044_91407_8_200_154_436 +--------AGACCTTTGG-ATACAA---------------------TGAACGACT----- +----- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTAC-----ACGACGCTCTTCCGATCT +>FC12044_91407_8_200_154_436 +AGAC-CTTTGGATACAATGAACGACT------------ +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTG------CTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_154_436 +------AGACCTT--TGGATACAATGAACGACT---------- +>Illumina_DpnII_Gex_Adapters1_1 +-GATCGTCGGACTGTAGAACTCTGAAC---- +>FC12044_91407_8_200_154_436 +AGACCTTTGGA---TACAA---TGAACGACT +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACA--GTCCGAC- +>FC12044_91407_8_200_154_436 +AGACCTTTGGA----TACAATGAACGACT +>Illumina_DpnII_Gex_Adapters2_1 +CAAGCAGAAGAC----GGCATACGA--------- +>FC12044_91407_8_200_154_436 +--------AGACCTTTGG-ATACAATGAACGACT +>Illumina_DpnII_Gex_Adapters2_2 +------TCGTATGC------CGTCTTCTGCTTG +>FC12044_91407_8_200_154_436 +AGACCTTTGGATACAATGAACGACT-------- +>Illumina_DpnII_Gex_PCR_Primer_1 +CAAGCAGAAGAC----GGCATACGA--------- +>FC12044_91407_8_200_154_436 +--------AGACCTTTGG-ATACAATGAACGACT +>Illumina_DpnII_Gex_PCR_Primer_2 +--------------AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_154_436 +AGACCTTTGGATACAATGA-ACGACT-------------------------------- +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTACA--GTCCGACGATC +>FC12044_91407_8_200_154_436 +--AGACCTTTGGA----TACAATGAACGACT--- +>Illumina_NlaIII_Gex_Adapters1_1 +------TCGGACTGTAGAACTCTGAAC---- +>FC12044_91407_8_200_154_436 +AGACCTTTGGA---TACAA---TGAACGACT +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACA--GTCCGACATG +>FC12044_91407_8_200_154_436 +AGACCTTTGGA----TACAATGAACGACT-- +>Illumina_NlaIII_Gex_Adapters2_1 +CAAGCAGAAGAC----GGCATACGANN------- +>FC12044_91407_8_200_154_436 +--------AGACCTTTGG-ATACAATGAACGACT +>Illumina_NlaIII_Gex_Adapters2_2 +------TCGTATGC------CGTCTTCTGCTTG +>FC12044_91407_8_200_154_436 +AGACCTTTGGATACAATGAACGACT-------- +>Illumina_NlaIII_Gex_PCR_Primer_1 +CAAGCAGAAGAC----GGCATACGA--------- +>FC12044_91407_8_200_154_436 +--------AGACCTTTGG-ATACAATGAACGACT +>Illumina_NlaIII_Gex_PCR_Primer_2 +--------------AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_154_436 +AGACCTTTGGATACAATGA-ACGACT-------------------------------- +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTACA--GTCCGACATG +>FC12044_91407_8_200_154_436 +---AGACCTTTGGA----TACAATGAACGACT-- +>Illumina_Small_RNA_RT_Primer +CAAGCAGAAGAC----GGCATACGA--------- +>FC12044_91407_8_200_154_436 +--------AGACCTTTGG-ATACAATGAACGACT +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTT----CTACA--GTCCGACGATC +>FC12044_91407_8_200_154_436 +----AGACCTTTGGATACAATGAACGACT--- +>Illumina_Small_RNA_3p_Adapter +------TCGTATGC------CGTCTTCTGCTTGT +>FC12044_91407_8_200_154_436 +AGACCTTTGGATACAATGAACGACT--------- +>Illumina_Small_RNA_PCR_Primer_1 +CAAGCAGAAGAC----GGCATACGA--------- +>FC12044_91407_8_200_154_436 +--------AGACCTTTGG-ATACAATGAACGACT +>Illumina_Small_RNA_PCR_Primer_2 +--------------AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_154_436 +AGACCTTTGGATACAATGA-ACGACT-------------------------------- +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTACA--GTCCGACGATC +>FC12044_91407_8_200_154_436 +--AGACCTTTGGA----TACAATGAACGACT--- +>Illumina_Genomici_DNA_Adapters1_1 +-----------GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_336_64 +AGGGAATTTTAGA---GGAGGGC-----TGCCG----------- +>Illumina_Genomic_DNA_Adapters1_2 +-----ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_336_64 +AGGGAA-----TTT---TAGAGGA-GGGCTGCCG---- +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAG---ATCTACACTCTTTCCCTACACGACGCTCTTCCGATC +T +>FC12044_91407_8_200_336_64 +-------------------AGGGAATTT------------TAGAGGA-GGGCTGCCG--- +- +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCAT-----ACGAGCTCTTCCGATCT +>FC12044_91407_8_200_336_64 +----------AGGGAATTTTAGAGGAGGGCTGCCG---- +>Illumina_Genomic_DNA_sequencing_primer +-----ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_336_64 +AGGGAA-----TTT---TAGAGGA-GGGCTGCCG---- +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGA---ATGCCGAG +>FC12044_91407_8_200_336_64 +-------AGGGAATTTTAG-AGGAGGGCTGCCG-- +>Illumina_Paired_End_DNA_Adapters1_2 +-----ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_336_64 +AGGGAA-----TTT---TAGAGGA-GGGCTGCCG---- +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAG---ATCTACACTCTTTCCCTACACGACGCTCTTCCGATC +T +>FC12044_91407_8_200_336_64 +-------------------AGGGAATTT------------TAGAGGA-GGGCTGCCG--- +- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCAT-----ACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTC +CGATCT +>FC12044_91407_8_200_336_64 +----------AGGGAATTTTAGAGGAG---GG-----------CTGCCG----------- +------ +>Illumina_Paired_End_DNA_sequencing_primer_1 +-----ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_336_64 +AGGGAA-----TTT---TAGAGGA-GGGCTGCCG---- +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCT---------GCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_336_64 +-----AGGGAATT--TTAGAGGAGGGCTG--CCG------------ +>Illumina_DpnII_Gex_Adapters1_1 +--------------GATCGTCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_336_64 +AGGGAATTTTAGAGGA-----GGGCTGCCG---------- +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAG-------TCCGAC +>FC12044_91407_8_200_336_64 +--AGG----GAATTTTAGAGGAGGGCTGCCG-- +>Illumina_DpnII_Gex_Adapters2_1 +CAAG-------CAGAAGACGGCATACGA +>FC12044_91407_8_200_336_64 +--AGGGAATTTTAGAGGAGGGCTGCCG- +>Illumina_DpnII_Gex_Adapters2_2 +------TCGTA---------TGCCGTCTTCTGCTTG +>FC12044_91407_8_200_336_64 +AGGGAATTTTAGAGGAGGGCTGCCG----------- +>Illumina_DpnII_Gex_PCR_Primer_1 +CAAG-------CAGAAGACGGCATACGA +>FC12044_91407_8_200_336_64 +--AGGGAATTTTAGAGGAGGGCTGCCG- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA--------- +>FC12044_91407_8_200_336_64 +---------------------AGG----GAATTTTAGAG---GAGGGCTGCCG +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTACAG-------TCCGACGATC +>FC12044_91407_8_200_336_64 +----AGG----GAATTTTAGAGGAGGGCTGCCG------ +>Illumina_NlaIII_Gex_Adapters1_1 +--------------TCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_336_64 +AGGGAATTTTAGAGGAGGGCTGCCG---------- +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAG-------TCCGACATG +>FC12044_91407_8_200_336_64 +--AGG----GAATTTTAGAGGAGGGCTGCCG----- +>Illumina_NlaIII_Gex_Adapters2_1 +CAAG-------CAGAAGACGGCATACGANN +>FC12044_91407_8_200_336_64 +--AGGGAATTTTAGAGGAGGGCTGCCG--- +>Illumina_NlaIII_Gex_Adapters2_2 +------TCGTA---------TGCCGTCTTCTGCTTG +>FC12044_91407_8_200_336_64 +AGGGAATTTTAGAGGAGGGCTGCCG----------- +>Illumina_NlaIII_Gex_PCR_Primer_1 +CAAG-------CAGAAGACGGCATACGA +>FC12044_91407_8_200_336_64 +--AGGGAATTTTAGAGGAGGGCTGCCG- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA--------- +>FC12044_91407_8_200_336_64 +---------------------AGG----GAATTTTAGAG---GAGGGCTGCCG +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTACAG-------TCCGACATG +>FC12044_91407_8_200_336_64 +-----AGG----GAATTTTAGAGGAGGGCTGCCG----- +>Illumina_Small_RNA_RT_Primer +CAAG-------CAGAAGACGGCATACGA +>FC12044_91407_8_200_336_64 +--AGGGAATTTTAGAGGAGGGCTGCCG- +>Illumina_Small_RNA_5p_Adapter +GTTCA--GAGTTCTACAG-------TCCGACGATC +>FC12044_91407_8_200_336_64 +----AGGGAATTTTAGAGGAGGGCTGCCG------ +>Illumina_Small_RNA_3p_Adapter +------TCGTA---------TGCCGTCTTCTGCTTGT +>FC12044_91407_8_200_336_64 +AGGGAATTTTAGAGGAGGGCTGCCG------------ +>Illumina_Small_RNA_PCR_Primer_1 +CAAG-------CAGAAGACGGCATACGA +>FC12044_91407_8_200_336_64 +--AGGGAATTTTAGAGGAGGGCTGCCG- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA--------- +>FC12044_91407_8_200_336_64 +---------------------AGG----GAATTTTAGAG---GAGGGCTGCCG +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTACAG-------TCCGACGATC +>FC12044_91407_8_200_336_64 +----AGG----GAATTTTAGAGGAGGGCTGCCG------ +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG----------------- +>FC12044_91407_8_200_620_233 +-------------------------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACG------ACGCTCTTCCGATCT- +>FC12044_91407_8_200_620_233 +----------TCTCCATGTTGGTCAGGCT-----GGTCTC +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTT--TCCCTACACGACGCTCTTCCGATCT +- +>FC12044_91407_8_200_620_233 +----------------------TCTCCA-TGTTGGTC--------AGGCT-----GGTCT +C +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT---------------- +>FC12044_91407_8_200_620_233 +-----------------------TCT--CCATGTTGGTCAGGCTGGTCTC +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACG------ACGCTCTTCCGATCT- +>FC12044_91407_8_200_620_233 +----------TCTCCATGTTGGTCAGGCT-----GGTCTC +>Illumina_Paired_End_DNA_Adapters1_1 +----------GATCGGAAGAGC-GGT-TCAGCAGGAATGCCGAG +>FC12044_91407_8_200_620_233 +TCTCCATGTTGGTCAG----GCTGGTCTC--------------- +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACG------ACGCTCTTCCGATCT- +>FC12044_91407_8_200_620_233 +----------TCTCCATGTTGGTCAGGCT-----GGTCTC +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTT--TCCCTACACGACGCTCTTCCGATCT +- +>FC12044_91407_8_200_620_233 +----------------------TCTCCA-TGTTGGTC--------AGGCT-----GGTCT +C +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCAT-----TCCTGCTGAACCG-CTCTT +CCGATCT +>FC12044_91407_8_200_620_233 +---------------------------TCTC--CATGTTGGTCAGGCTG----GTCTC-- +------- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACG------ACGCTCTTCCGATCT- +>FC12044_91407_8_200_620_233 +----------TCTCCATGTTGGTCAGGCT-----GGTCTC +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCAT-----TCCTGCTGAACCG-CTCTTCCGATCT +>FC12044_91407_8_200_620_233 +---TCTC--CATGTTGGTCAGGCTG----GTCTC--------- +>Illumina_DpnII_Gex_Adapters1_1 +-------GATCGTCGGACT-GTAGAACTCTGAAC +>FC12044_91407_8_200_620_233 +TCTCCATGTTGGTCAGGCTGGT----CTC----- +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACA-----GTCCGAC------- +>FC12044_91407_8_200_620_233 +-------------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_DpnII_Gex_Adapters2_1 +-------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_620_233 +TCTCCATGTTGGTCAGGCTGGTCTC--------- +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG----------------- +>FC12044_91407_8_200_620_233 +-------------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_DpnII_Gex_PCR_Primer_1 +-------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_620_233 +TCTCCATGTTGGTCAGGCTGGTCTC--------- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACA-----GTCCGA-------- +>FC12044_91407_8_200_620_233 +--------------------------------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTACA-----GTCCGAC-GATC-- +>FC12044_91407_8_200_620_233 +---------------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_NlaIII_Gex_Adapters1_1 +------------TCGGACT-GTAGAACTCTGAAC +>FC12044_91407_8_200_620_233 +TCTCCATGTTGGTCAGGCTGGT----CTC----- +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACA-----GTCCGACATG----- +>FC12044_91407_8_200_620_233 +-------------TCTCCATGTTGGTCAGGC-TGGTCTC +>Illumina_NlaIII_Gex_Adapters2_1 +-------------CAAGCAGAAGACGGCATACGANN +>FC12044_91407_8_200_620_233 +TCTCCATGTTGGTCAGGCTGGTCTC----------- +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG----------------- +>FC12044_91407_8_200_620_233 +-------------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_NlaIII_Gex_PCR_Primer_1 +-------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_620_233 +TCTCCATGTTGGTCAGGCTGGTCTC--------- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACA-----GTCCGA-------- +>FC12044_91407_8_200_620_233 +--------------------------------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTACA-----GTCCGACATG----- +>FC12044_91407_8_200_620_233 +----------------TCTCCATGTTGGTCAGGC-TGGTCTC +>Illumina_Small_RNA_RT_Primer +-------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_620_233 +TCTCCATGTTGGTCAGGCTGGTCTC--------- +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTACA-----GTCCGAC-GATC-- +>FC12044_91407_8_200_620_233 +---------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTCTTCTGCTTGT---------------- +>FC12044_91407_8_200_620_233 +-------------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_Small_RNA_PCR_Primer_1 +-------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_620_233 +TCTCCATGTTGGTCAGGCTGGTCTC--------- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACA-----GTCCGA-------- +>FC12044_91407_8_200_620_233 +--------------------------------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTACA-----GTCCGAC-GATC-- +>FC12044_91407_8_200_620_233 +---------------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_Genomici_DNA_Adapters1_1 +----------GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_902_349 +TGAACGTCGAGA-CGCAAG-GCCC----GCC------------ +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACAC-----GACGC----TCTTCCGATCT +>FC12044_91407_8_200_902_349 +------------TGAACGTCGAGACGCAAGGCCCGCC----- +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGA----------CCACCGAGATCTACACTCTTTCCCTACACGACGCTCT +TCCGATCT +>FC12044_91407_8_200_902_349 +--TGA-ACGTCGAGACGCAAGGCCCGCC-------------------------------- +-------- +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGA------AGACGGCATACGAGCTCTTCCGATCT +>FC12044_91407_8_200_902_349 +-----TGAACGTCGAGACG-----CAAGGCCCGCC----- +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACAC-----GACGC----TCTTCCGATCT +>FC12044_91407_8_200_902_349 +------------TGAACGTCGAGACGCAAGGCCCGCC----- +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG-------------- +>FC12044_91407_8_200_902_349 +---------------------TGAACGTCGAGACGCAAGGCCCGCC +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACAC-----GACGC----TCTTCCGATCT +>FC12044_91407_8_200_902_349 +------------TGAACGTCGAGACGCAAGGCCCGCC----- +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGA----------CCACCGAGATCTACACTCTTTCCCTACACGACGCTCT +TCCGATCT +>FC12044_91407_8_200_902_349 +--TGA-ACGTCGAGACGCAAGGCCCGCC-------------------------------- +-------- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCG---GTCTCGGCATTCCTGCTGAACCGCTCTTCCG +ATCT +>FC12044_91407_8_200_902_349 +-----TGA--ACG----TCGAGA-CGCAAGGCCCGCC----------------------- +---- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACAC-----GACGC----TCTTCCGATCT +>FC12044_91407_8_200_902_349 +------------TGAACGTCGAGACGCAAGGCCCGCC----- +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGCTGAA----------------CCGCTCTTCCGATCT +>FC12044_91407_8_200_902_349 +------------------TGAACGTCGAGACGCAAGGCCCGCC---------- +>Illumina_DpnII_Gex_Adapters1_1 +-GATCGTCGGACTGTAGAACTCTGAAC------- +>FC12044_91407_8_200_902_349 +TGAACGTCG------AGA---CGCAAGGCCCGCC +>Illumina_DpnII_Gex_Adapters1_2 +---ACAGGTTCAGA-GTTCTACA--GTCCGAC +>FC12044_91407_8_200_902_349 +TGAAC--GTCGAGACG-----CAAGGCCCGCC +>Illumina_DpnII_Gex_Adapters2_1 +--------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACGCAAG-----GCCCGCC----- +>Illumina_DpnII_Gex_Adapters2_2 +-----------TCGTATG--CCGTCTTCTGCTTG +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACGCAAGGCCCGCC--------- +>Illumina_DpnII_Gex_PCR_Primer_1 +--------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACGCAAG-----GCCCGCC----- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGA----------CCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_902_349 +--TGA-ACGTCGAGACGCAAGGCCCGCC-------------------------- +>Illumina_DpnII_Gex_sequencing_primer +-------CGACAGGTTCAGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACG--CA-AG-------GCCCGCC---- +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTGTAGAACTCTGAAC-------------------- +>FC12044_91407_8_200_902_349 +----------------TGAACGTCGAGACGCAAGGCCCGCC +>Illumina_NlaIII_Gex_Adapters1_2 +---ACAGGTTCAGA-GTTCTACA--GTCCGACATG +>FC12044_91407_8_200_902_349 +TGAAC--GTCGAGACG-----CAAGGCCCGCC--- +>Illumina_NlaIII_Gex_Adapters2_1 +--------------CAAGCAGAAGACGGCATACGANN +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACGCAAG-----GCCCGCC------- +>Illumina_NlaIII_Gex_Adapters2_2 +-----------TCGTATG--CCGTCTTCTGCTTG +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACGCAAGGCCCGCC--------- +>Illumina_NlaIII_Gex_PCR_Primer_1 +--------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACGCAAG-----GCCCGCC----- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGA----------CCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_902_349 +--TGA-ACGTCGAGACGCAAGGCCCGCC-------------------------- +>Illumina_NlaIII_Gex_sequencing_primer +------CCGACAGGTTCAGAGTTCTACAGTCCGACATG +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACG--CA-AG-------GCCCGCC--- +>Illumina_Small_RNA_RT_Primer +--------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACGCAAG-----GCCCGCC----- +>Illumina_Small_RNA_5p_Adapter +-----GTTCAGA-GTTCTACA--GTCCGACGATC +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACG-----CAAGGCCCGCC---- +>Illumina_Small_RNA_3p_Adapter +-----------TCGTATG--CCGTCTTCTGCTTGT +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACGCAAGGCCCGCC---------- +>Illumina_Small_RNA_PCR_Primer_1 +--------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACGCAAG-----GCCCGCC----- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGA----------CCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_902_349 +--TGA-ACGTCGAGACGCAAGGCCCGCC-------------------------- +>Illumina_Small_RNA_sequencing_primer +-------CGACAGGTTCAGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACG--CA-AG-------GCCCGCC---- +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGTATGCCGTCTTC----------TGCTTG- +>FC12044_91407_8_200_40_618 +-------------------CTGTCCCCACGGCGGGGGGGCCTGG +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACG-------CTCTTCCGATCT +>FC12044_91407_8_200_40_618 +-----CTGTCCC--CACGGCGGGGGGGCCTGG-------- +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG-------CTCTTCC +GATCT +>FC12044_91407_8_200_40_618 +------------------------------CTGTCCC--CACGGCGGGGGGGCCTGG--- +----- +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT----------------------- +>FC12044_91407_8_200_40_618 +--------------------------------CTGTCCCCACGGCGGGGGGGCCTGG +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGACG-------CTCTTCCGATCT +>FC12044_91407_8_200_40_618 +-----CTGTCCC--CACGGCGGGGGGGCCTGG-------- +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGT----TCAGCAGGAATGCCGAG +>FC12044_91407_8_200_40_618 +-----------CTGTCCCCACGGCGGGGGGGCCTGG +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACG-------CTCTTCCGATCT +>FC12044_91407_8_200_40_618 +-----CTGTCCC--CACGGCGGGGGGGCCTGG-------- +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG-------CTCTTCC +GATCT +>FC12044_91407_8_200_40_618 +------------------------------CTGTCCC--CACGGCGGGGGGGCCTGG--- +----- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTC-----------GGCATTCCTGCTGAACCG +CTCTTCCGATCT +>FC12044_91407_8_200_40_618 +------------------------CTGTCCCCACGGCGGGGGGG----CCTGG------- +------------ +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGACG-------CTCTTCCGATCT +>FC12044_91407_8_200_40_618 +-----CTGTCCC--CACGGCGGGGGGGCCTGG-------- +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTC-----------GGCATTCCTGCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_40_618 +CTGTCCCCACGGCGGGGGGG----CCTGG------------------- +>Illumina_DpnII_Gex_Adapters1_1 +GATCGTCGGACTGTAGAACTCTGAAC------------------- +>FC12044_91407_8_200_40_618 +--------------------CTGTCCCCACGGCGGGGGGGCCTGG +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAGT-CCGAC--------------- +>FC12044_91407_8_200_40_618 +-----------------CTGTCCCCACGGCGGGGGGGCCTGG +>Illumina_DpnII_Gex_Adapters2_1 +-------CAAGCAGAAGACGGCATACGA---------- +>FC12044_91407_8_200_40_618 +CTGTCCCC---------ACGG----CGGGGGGGCCTGG +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTCTTC----------TGCTTG- +>FC12044_91407_8_200_40_618 +-------CTGTCCCCACGGCGGGGGGGCCTGG +>Illumina_DpnII_Gex_PCR_Primer_1 +-------CAAGCAGAAGACGGCATACGA---------- +>FC12044_91407_8_200_40_618 +CTGTCCCC---------ACGG----CGGGGGGGCCTGG +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA---------------- +- +>FC12044_91407_8_200_40_618 +------------------------------------CTGTCCCCACGGCGGGGGGGCCTG +G +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTACAGTCCGACGATC------------------ +>FC12044_91407_8_200_40_618 +-------------------CTGTCC------CCACGGCGGGGGGGCCTGG +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTGTAGAACTCTGAAC------------------- +>FC12044_91407_8_200_40_618 +---------------CTGTCCCCACGGCGGGGGGGCCTGG +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAGTCCGACATG-------------- +>FC12044_91407_8_200_40_618 +-----------------CTGTCC-CCACGGCGGGGGGGCCTGG +>Illumina_NlaIII_Gex_Adapters2_1 +---------------------CAAGCAGAAGACGGCATACGANN +>FC12044_91407_8_200_40_618 +CTGTCCCCACGGCGGGGGGGCCTGG------------------- +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTCTTC----------TGCTTG- +>FC12044_91407_8_200_40_618 +-------CTGTCCCCACGGCGGGGGGGCCTGG +>Illumina_NlaIII_Gex_PCR_Primer_1 +-------CAAGCAGAAGACGGCATACGA---------- +>FC12044_91407_8_200_40_618 +CTGTCCCC---------ACGG----CGGGGGGGCCTGG +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA---------------- +- +>FC12044_91407_8_200_40_618 +------------------------------------CTGTCCCCACGGCGGGGGGGCCTG +G +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTACAGTCCGACATG-------------- +>FC12044_91407_8_200_40_618 +--------------------CTGTCC-CCACGGCGGGGGGGCCTGG +>Illumina_Small_RNA_RT_Primer +-------CAAGCAGAAGACGGCATACGA---------- +>FC12044_91407_8_200_40_618 +CTGTCCCC---------ACGG----CGGGGGGGCCTGG +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTACAGTCCGACGATC------------------ +>FC12044_91407_8_200_40_618 +-------------CTGTCC------CCACGGCGGGGGGGCCTGG +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTCTTCTGCTTGT--------------------- +>FC12044_91407_8_200_40_618 +------------------CTGTCCCCACGGCGGGGGGGCCTGG +>Illumina_Small_RNA_PCR_Primer_1 +-------CAAGCAGAAGACGGCATACGA---------- +>FC12044_91407_8_200_40_618 +CTGTCCCC---------ACGG----CGGGGGGGCCTGG +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA---------------- +- +>FC12044_91407_8_200_40_618 +------------------------------------CTGTCCCCACGGCGGGGGGGCCTG +G +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTACAGTCCGACGATC------------------ +>FC12044_91407_8_200_40_618 +-------------------CTGTCC------CCACGGCGGGGGGGCCTGG +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGTATGCC--GTCTTCTGCTTG +>FC12044_91407_8_200_83_511 +-----GATGTACTCTTACACCCAGACTT-TG---- +>Illumina_Genomic_DNA_Adapters1_2 +---ACACTCTTTCCCTACAC---GAC---GCTCTTCCGATCT +>FC12044_91407_8_200_83_511 +GATGTACTCT-----TACACCCAGACTTTG------------ +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACAC---GAC---GCTCTTCCG +ATCT +>FC12044_91407_8_200_83_511 +--------------------GATGTACTCT-------TACACCCAGACTTTG-------- +---- +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGCTCTT-----CCGATCT--- +>FC12044_91407_8_200_83_511 +---------GATG---TA----CTCTTACACCCAGA-CTTTG +>Illumina_Genomic_DNA_sequencing_primer +---ACACTCTTTCCCTACAC---GAC---GCTCTTCCGATCT +>FC12044_91407_8_200_83_511 +GATGTACTCT-----TACACCCAGACTTTG------------ +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAATG----------CCGAG------ +>FC12044_91407_8_200_83_511 +-----------------------GATGTACTCTTACACCCAGACTTTG +>Illumina_Paired_End_DNA_Adapters1_2 +---ACACTCTTTCCCTACAC---GAC---GCTCTTCCGATCT +>FC12044_91407_8_200_83_511 +GATGTACTCT-----TACACCCAGACTTTG------------ +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACAC---GAC---GCTCTTCCG +ATCT +>FC12044_91407_8_200_83_511 +--------------------GATGTACTCT-------TACACCCAGACTTTG-------- +---- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTT-----C +CGATCT--- +>FC12044_91407_8_200_83_511 +----------------------------------------GATGTA---CTCTTACACCC +AGA-CTTTG +>Illumina_Paired_End_DNA_sequencing_primer_1 +---ACACTCTTTCCCTACAC---GAC---GCTCTTCCGATCT +>FC12044_91407_8_200_83_511 +GATGTACTCT-----TACACCCAGACTTTG------------ +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGCTGAACCGCTCTT-----CCGATCT--- +>FC12044_91407_8_200_83_511 +----------------GATGTA---CTCTTACACCCAGA-CTTTG +>Illumina_DpnII_Gex_Adapters1_1 +GATCGTCGGACTGTAGAACTCTGA-AC---------- +>FC12044_91407_8_200_83_511 +--------GA-TGT---ACTCTTACACCCAGACTTTG +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGA-GTTC---TACAGTCCGAC---- +>FC12044_91407_8_200_83_511 +---------GATGTACTCTTACACCCAGACTTTG +>Illumina_DpnII_Gex_Adapters2_1 +------------CAAGCAGA---AGACGGCATACGA +>FC12044_91407_8_200_83_511 +GATGTACTCTTACACCCAGACTTTG----------- +>Illumina_DpnII_Gex_Adapters2_2 +-------TCGTATGCC--GTCTTCTGCTTG +>FC12044_91407_8_200_83_511 +GATGTACTCTTACACCCAGACTT-TG---- +>Illumina_DpnII_Gex_PCR_Primer_1 +------------CAAGCAGA---AGACGGCATACGA +>FC12044_91407_8_200_83_511 +GATGTACTCTTACACCCAGACTTTG----------- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGA-GTTC---TACAGTCCGA----- +>FC12044_91407_8_200_83_511 +----------------------------GATGTACTCTTACACCCAGACTTTG +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGA-GTTC---TACAGTCCGAC---GATC +>FC12044_91407_8_200_83_511 +-----------GATGTACTCTTACACCCAGACTTTG--- +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTGTAGAACTCTGA-AC---------- +>FC12044_91407_8_200_83_511 +---GA-TGT---ACTCTTACACCCAGACTTTG +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGA-GTTC---TACAGTCCGACATG- +>FC12044_91407_8_200_83_511 +---------GATGTACTCTTACACCCAGACTTTG +>Illumina_NlaIII_Gex_Adapters2_1 +------------CAAGCAGA---AGACGGCATACGANN +>FC12044_91407_8_200_83_511 +GATGTACTCTTACACCCAGACTTTG------------- +>Illumina_NlaIII_Gex_Adapters2_2 +-------TCGTATGCC--GTCTTCTGCTTG +>FC12044_91407_8_200_83_511 +GATGTACTCTTACACCCAGACTT-TG---- +>Illumina_NlaIII_Gex_PCR_Primer_1 +------------CAAGCAGA---AGACGGCATACGA +>FC12044_91407_8_200_83_511 +GATGTACTCTTACACCCAGACTTTG----------- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGA-GTTC---TACAGTCCGA----- +>FC12044_91407_8_200_83_511 +----------------------------GATGTACTCTTACACCCAGACTTTG +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGA-GTTC---TACAGTCCGACATG- +>FC12044_91407_8_200_83_511 +------------GATGTACTCTTACACCCAGACTTTG +>Illumina_Small_RNA_RT_Primer +------------CAAGCAGA---AGACGGCATACGA +>FC12044_91407_8_200_83_511 +GATGTACTCTTACACCCAGACTTTG----------- +>Illumina_Small_RNA_5p_Adapter +GTTCAGA-GTTC---TACAGTCCGAC---GATC +>FC12044_91407_8_200_83_511 +-----GATGTACTCTTACACCCAGACTTTG--- +>Illumina_Small_RNA_3p_Adapter +-------TCGTATGCC--GTCTTCTGCTTGT +>FC12044_91407_8_200_83_511 +GATGTACTCTTACACCCAGACTT-TG----- +>Illumina_Small_RNA_PCR_Primer_1 +------------CAAGCAGA---AGACGGCATACGA +>FC12044_91407_8_200_83_511 +GATGTACTCTTACACCCAGACTTTG----------- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGA-GTTC---TACAGTCCGA----- +>FC12044_91407_8_200_83_511 +----------------------------GATGTACTCTTACACCCAGACTTTG +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGA-GTTC---TACAGTCCGAC---GATC +>FC12044_91407_8_200_83_511 +-----------GATGTACTCTTACACCCAGACTTTG--- +>Illumina_Genomici_DNA_Adapters1_1 +---------GATC--GGAAGAGCTC---GTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_76_246 +TCAAGGGTGGATCTTG-----GCTCCCAGT----------------- +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTTC------CGATCT----------- +>FC12044_91407_8_200_76_246 +-------------------------TCAAGGGTGGATCTTGGCTCCCAGT +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTC------CG +ATCT----------- +>FC12044_91407_8_200_76_246 +--------------------------------------------------TCAAGGGTGG +ATCTTGGCTCCCAGT +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGCTCTTC------CGATCT----------- +>FC12044_91407_8_200_76_246 +--------------------------TCAAGGGTGGATCTTGGCTCCCAGT +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGACGCTCTTC------CGATCT----------- +>FC12044_91407_8_200_76_246 +-------------------------TCAAGGGTGGATCTTGGCTCCCAGT +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAATG---CCGAG- +>FC12044_91407_8_200_76_246 +--TC--AAGGGTGGATC-------TTGGCTCCCAGT +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTTC------CGATCT----------- +>FC12044_91407_8_200_76_246 +-------------------------TCAAGGGTGGATCTTGGCTCCCAGT +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTC------CG +ATCT----------- +>FC12044_91407_8_200_76_246 +--------------------------------------------------TCAAGGGTGG +ATCTTGGCTCCCAGT +>Illumina_Paired_End_DNA_PCR_Primers1_2 +-CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCC---TGCTGAACCGCTCTTCC +GATCT +>FC12044_91407_8_200_76_246 +TCAAG-----------------GGTGGATCTTGGC--TCCCAGT---------------- +----- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGACGCTCTTC------CGATCT----------- +>FC12044_91407_8_200_76_246 +-------------------------TCAAGGGTGGATCTTGGCTCCCAGT +>Illumina_Paired_End_DNA_sequencing_primer_2 +--------CGGTCTCGGCATTCC---TGCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_76_246 +TCAAGGGTGGATCTTGGC--TCCCAGT--------------------- +>Illumina_DpnII_Gex_Adapters1_1 +---------GATCGTCGGACT----GTAGAACTCTGAAC +>FC12044_91407_8_200_76_246 +TCAAGGGTGGATCTT--GGCTCCCAGT------------ +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTC-AGAGT---TCT------ACAGTCCGAC +>FC12044_91407_8_200_76_246 +------TCAAGGGTGGATCTTGGCTCCCAGT----- +>Illumina_DpnII_Gex_Adapters2_1 +-CAAG----------------CAGAAGACGGCATACGA +>FC12044_91407_8_200_76_246 +TCAAGGGTGGATCTTGGCTCCCAGT------------- +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTCTTC-----TG---CTTG--------- +>FC12044_91407_8_200_76_246 +-------------TCAAGGGTGGATCTTGGCTCCCAGT +>Illumina_DpnII_Gex_PCR_Primer_1 +-CAAG----------------CAGAAGACGGCATACGA +>FC12044_91407_8_200_76_246 +TCAAGGGTGGATCTTGGCTCCCAGT------------- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTC-AGAGT---TCT------ACAGTCCGA +>FC12044_91407_8_200_76_246 +-------------------------TCAAGGGTGGATCTTGGCTCCCAGT---- +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTC-AGAGT---TCT------ACAGTCCGACGATC +>FC12044_91407_8_200_76_246 +--------TCAAGGGTGGATCTTGGCTCCCAGT--------- +>Illumina_NlaIII_Gex_Adapters1_1 +TC--GGACTGTAGAACTCTGAAC----- +>FC12044_91407_8_200_76_246 +TCAAGG---GTGGATCTTGGCTCCCAGT +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTC-AGAGT---TCT------ACAGTCCGACATG +>FC12044_91407_8_200_76_246 +------TCAAGGGTGGATCTTGGCTCCCAGT-------- +>Illumina_NlaIII_Gex_Adapters2_1 +-CAAG----------------CAGAAGACGGCATACGANN +>FC12044_91407_8_200_76_246 +TCAAGGGTGGATCTTGGCTCCCAGT--------------- +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTCTTC-----TG---CTTG--------- +>FC12044_91407_8_200_76_246 +-------------TCAAGGGTGGATCTTGGCTCCCAGT +>Illumina_NlaIII_Gex_PCR_Primer_1 +-CAAG----------------CAGAAGACGGCATACGA +>FC12044_91407_8_200_76_246 +TCAAGGGTGGATCTTGGCTCCCAGT------------- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTC-AGAGT---TCT------ACAGTCCGA +>FC12044_91407_8_200_76_246 +-------------------------TCAAGGGTGGATCTTGGCTCCCAGT---- +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTC-AGAGT---TCT------ACAGTCCGACATG +>FC12044_91407_8_200_76_246 +---------TCAAGGGTGGATCTTGGCTCCCAGT-------- +>Illumina_Small_RNA_RT_Primer +-CAAG----------------CAGAAGACGGCATACGA +>FC12044_91407_8_200_76_246 +TCAAGGGTGGATCTTGGCTCCCAGT------------- +>Illumina_Small_RNA_5p_Adapter +GTTC-AGAGT---TCT------ACAGTCCGACGATC +>FC12044_91407_8_200_76_246 +--TCAAGGGTGGATCTTGGCTCCCAGT--------- +>Illumina_Small_RNA_3p_Adapter +TC------GTATGCCGTCTT--CTGCTTGT +>FC12044_91407_8_200_76_246 +TCAAGGGTGGA-----TCTTGGCTCCCAGT +>Illumina_Small_RNA_PCR_Primer_1 +-CAAG----------------CAGAAGACGGCATACGA +>FC12044_91407_8_200_76_246 +TCAAGGGTGGATCTTGGCTCCCAGT------------- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTC-AGAGT---TCT------ACAGTCCGA +>FC12044_91407_8_200_76_246 +-------------------------TCAAGGGTGGATCTTGGCTCCCAGT---- +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTC-AGAGT---TCT------ACAGTCCGACGATC +>FC12044_91407_8_200_76_246 +--------TCAAGGGTGGATCTTGGCTCCCAGT--------- +>Illumina_Genomici_DNA_Adapters1_1 +GAT--CGGAAGAGCTCGTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_303_427 +--TTGCGACAGAG---TTTTGC--TCTTGTCC--- +>Illumina_Genomic_DNA_Adapters1_2 +-----ACA--------CTCTT-TCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_303_427 +TTGCGACAGAGTTTTGCTCTTGTCC---------------------- +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTT-TCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_303_427 +-------TTGCGAC-----AGAGTTTTGCTCTTGTCC---------------------- +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGA---------GCTCT--TCCGATCT +>FC12044_91407_8_200_303_427 +---------------TTGCGACAGAGTTTTGCTCTTGTCC----- +>Illumina_Genomic_DNA_sequencing_primer +-----ACA--------CTCTT-TCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_303_427 +TTGCGACAGAGTTTTGCTCTTGTCC---------------------- +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG-------------- +>FC12044_91407_8_200_303_427 +--------------TT--GC--GA---CAGAGTTTTGCTCTTGTCC +>Illumina_Paired_End_DNA_Adapters1_2 +-----ACA--------CTCTT-TCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_303_427 +TTGCGACAGAGTTTTGCTCTTGTCC---------------------- +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTT-TCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_303_427 +-------TTGCGAC-----AGAGTTTTGCTCTTGTCC---------------------- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCA----TTCCTGCTGAACCGCTCTTCC +GATCT +>FC12044_91407_8_200_303_427 +---------------------------TTGCGACAGAGTTT--TGCT------CTTGTCC +----- +>Illumina_Paired_End_DNA_sequencing_primer_1 +-----ACA--------CTCTT-TCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_303_427 +TTGCGACAGAGTTTTGCTCTTGTCC---------------------- +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCA----TTCCTGCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_303_427 +---TTGCGACAGAGTTT--TGCT------CTTGTCC----- +>Illumina_DpnII_Gex_Adapters1_1 +GAT--CGTCGGACTGTAGAACTCTGAAC- +>FC12044_91407_8_200_303_427 +--TTGCGACAGAGTTTTG--CTCTTGTCC +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTT----CAGAGTT---CTACAGTCCGAC +>FC12044_91407_8_200_303_427 +-----TTGCGACAGAGTTTTGCTCTTGTCC--- +>Illumina_DpnII_Gex_Adapters2_1 +CAAGCAGAAGACGGCATACGA------------------- +>FC12044_91407_8_200_303_427 +---------------TTGCGACAGAGTTTTGCTCTTGTCC +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTC----TTCTG--CTTG--- +>FC12044_91407_8_200_303_427 +-----TTGCGACAGAGTTTTGCTCTTGTCC +>Illumina_DpnII_Gex_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA------------------- +>FC12044_91407_8_200_303_427 +---------------TTGCGACAGAGTTTTGCTCTTGTCC +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTT----CAGAGTT---CTACAGTCCGA +>FC12044_91407_8_200_303_427 +------------------------TTGCGACAGAGTTTTGCTCTTGTCC-- +>Illumina_DpnII_Gex_sequencing_primer +---CGACAGGTTCAGAGTT---CTACAGTCCGACGATC +>FC12044_91407_8_200_303_427 +TTGCGA------CAGAGTTTTGCTCTTGTCC------- +>Illumina_NlaIII_Gex_Adapters1_1 +-TCGGACTGTAGAACTCTGAAC------ +>FC12044_91407_8_200_303_427 +TTGCGAC---AGAGTTTTGCTCTTGTCC +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTT----CAGAGTT---CTACAGTCCGACATG +>FC12044_91407_8_200_303_427 +-----TTGCGACAGAGTTTTGCTCTTGTCC------ +>Illumina_NlaIII_Gex_Adapters2_1 +CAAGCAGAAGACGGCATACGANN----------------- +>FC12044_91407_8_200_303_427 +---------------TTGCGACAGAGTTTTGCTCTTGTCC +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTC----TTCTG--CTTG--- +>FC12044_91407_8_200_303_427 +-----TTGCGACAGAGTTTTGCTCTTGTCC +>Illumina_NlaIII_Gex_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA------------------- +>FC12044_91407_8_200_303_427 +---------------TTGCGACAGAGTTTTGCTCTTGTCC +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTT----CAGAGTT---CTACAGTCCGA +>FC12044_91407_8_200_303_427 +------------------------TTGCGACAGAGTTTTGCTCTTGTCC-- +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTT----CAGAGTT---CTACAGTCCGACATG +>FC12044_91407_8_200_303_427 +--------TTGCGACAGAGTTTTGCTCTTGTCC------ +>Illumina_Small_RNA_RT_Primer +CAAGCAGAAGACGGCATACGA------------------- +>FC12044_91407_8_200_303_427 +---------------TTGCGACAGAGTTTTGCTCTTGTCC +>Illumina_Small_RNA_5p_Adapter +GTT----CAGAGTT---CTACAGTCCGACGATC +>FC12044_91407_8_200_303_427 +-TTGCGACAGAGTTTTGCTCTTGTCC------- +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTC----TTCTG--CTTGT-- +>FC12044_91407_8_200_303_427 +-----TTGCGACAGAGTTTTGCTCTTGTCC +>Illumina_Small_RNA_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA------------------- +>FC12044_91407_8_200_303_427 +---------------TTGCGACAGAGTTTTGCTCTTGTCC +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTT----CAGAGTT---CTACAGTCCGA +>FC12044_91407_8_200_303_427 +------------------------TTGCGACAGAGTTTTGCTCTTGTCC-- +>Illumina_Small_RNA_sequencing_primer +---CGACAGGTTCAGAGTT---CTACAGTCCGACGATC +>FC12044_91407_8_200_303_427 +TTGCGA------CAGAGTTTTGCTCTTGTCC------- +>Illumina_Genomici_DNA_Adapters1_1 +---GATCGGAAGAGCTC---GTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_31_299 +TCTGCTC----CAGCTCCAAG-ACGCCGCC--------- +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCT-TTCC--CTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_31_299 +----TCTGCTCCAGCTCCAAGACGC-CGCC------ +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCC--CTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_31_299 +----------------------TCTGC------TCCAGCTCCAAGACGC-CGCC------ +>Illumina_Genomic_DNA_PCR_Primers1_2 +------CAAGC----AGAAGACGGCATACGAGCTCTTCCGATCT +>FC12044_91407_8_200_31_299 +TCTGCTCCAGCTCCAAGACGCCGCC------------------- +>Illumina_Genomic_DNA_sequencing_primer +ACACTCT-TTCC--CTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_31_299 +----TCTGCTCCAGCTCCAAGACGC-CGCC------ +>Illumina_Paired_End_DNA_Adapters1_1 +---GATCGGAAGAGCGGTTCAGC----AGGAATGCCGAG +>FC12044_91407_8_200_31_299 +TCTGCTC------------CAGCTCCAAG--ACGCCGCC +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCT-TTCC--CTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_31_299 +----TCTGCTCCAGCTCCAAGACGC-CGCC------ +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCC--CTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_31_299 +----------------------TCTGC------TCCAGCTCCAAGACGC-CGCC------ +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAA-----CCGCTCTTC +CGATCT +>FC12044_91407_8_200_31_299 +-----------------------------TCTGC--TCCAGCTCCAAGACGCCGCC---- +------ +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCT-TTCC--CTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_31_299 +----TCTGCTCCAGCTCCAAGACGC-CGCC------ +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGCTGAA-----CCGCTCTTCCGATCT +>FC12044_91407_8_200_31_299 +-----TCTGC--TCCAGCTCCAAGACGCCGCC---------- +>Illumina_DpnII_Gex_Adapters1_1 +GATCGTCGGACTGTAGAACTCTGAAC------------------ +>FC12044_91407_8_200_31_299 +-------------------TCTGCTCCAGCTCCAAGACGCCGCC +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAG-TCC--GAC------ +>FC12044_91407_8_200_31_299 +------TC----TGCTCCAGCTCCAAGACGCCGCC +>Illumina_DpnII_Gex_Adapters2_1 +------CAAGC----AGAAGACGGCATACGA +>FC12044_91407_8_200_31_299 +TCTGCTCCAGCTCCAAGACGCCGCC------ +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG----------------- +>FC12044_91407_8_200_31_299 +-------------TCTGCTCCAGCTCCAAGACGCCGCC +>Illumina_DpnII_Gex_PCR_Primer_1 +------CAAGC----AGAAGACGGCATACGA +>FC12044_91407_8_200_31_299 +TCTGCTCCAGCTCCAAGACGCCGCC------ +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA--------- +>FC12044_91407_8_200_31_299 +-------------------------TC----TGCTCCAGCTCCAAGACGCCGCC +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTACAG-TCC--GACGATC---- +>FC12044_91407_8_200_31_299 +--------TC----TGCTCCAGCTCCAAGACG--CCGCC +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTGTAGAACTCTGAAC------------------ +>FC12044_91407_8_200_31_299 +--------------TCTGCTCCAGCTCCAAGACGCCGCC +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAG-TCCGACATG----- +>FC12044_91407_8_200_31_299 +------TC----TGCTCCAGCTCCAAGACGCCGCC +>Illumina_NlaIII_Gex_Adapters2_1 +------CAAGC----AGAAGACGGCATACGANN +>FC12044_91407_8_200_31_299 +TCTGCTCCAGCTCCAAGACGCCGCC-------- +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG----------------- +>FC12044_91407_8_200_31_299 +-------------TCTGCTCCAGCTCCAAGACGCCGCC +>Illumina_NlaIII_Gex_PCR_Primer_1 +------CAAGC----AGAAGACGGCATACGA +>FC12044_91407_8_200_31_299 +TCTGCTCCAGCTCCAAGACGCCGCC------ +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA--------- +>FC12044_91407_8_200_31_299 +-------------------------TC----TGCTCCAGCTCCAAGACGCCGCC +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTACAG-TCCGACATG----- +>FC12044_91407_8_200_31_299 +---------TC----TGCTCCAGCTCCAAGACGCCGCC +>Illumina_Small_RNA_RT_Primer +------CAAGC----AGAAGACGGCATACGA +>FC12044_91407_8_200_31_299 +TCTGCTCCAGCTCCAAGACGCCGCC------ +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTACAG-TCC--GACGATC---- +>FC12044_91407_8_200_31_299 +--TC----TGCTCCAGCTCCAAGACG--CCGCC +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTCTTCTGCTTGT------------------- +>FC12044_91407_8_200_31_299 +-------------TCTGC---TCCAGCTCCAAGACGCCGCC +>Illumina_Small_RNA_PCR_Primer_1 +------CAAGC----AGAAGACGGCATACGA +>FC12044_91407_8_200_31_299 +TCTGCTCCAGCTCCAAGACGCCGCC------ +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA--------- +>FC12044_91407_8_200_31_299 +-------------------------TC----TGCTCCAGCTCCAAGACGCCGCC +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTACAG-TCC--GACGATC---- +>FC12044_91407_8_200_31_299 +--------TC----TGCTCCAGCTCCAAGACG--CCGCC +>Illumina_Genomici_DNA_Adapters1_1 +----GATC--------GGAAGAGCTCGTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGGGAAAGGCG-------------------- +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTAC----------------ACGACGCTCTTCCGATCT +>FC12044_91407_8_200_553_135 +------------TACGGAGCCGCGGGCGGGAAAGGCG------------ +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCAC---CGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC +T +>FC12044_91407_8_200_553_135 +-----TACGGAG-CCGCGGGCGGGA------------------AAGGCG----------- +- +>Illumina_Genomic_DNA_PCR_Primers1_2 +---CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGGGAAAGGCG------------ +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTAC----------------ACGACGCTCTTCCGATCT +>FC12044_91407_8_200_553_135 +------------TACGGAGCCGCGGGCGGGAAAGGCG------------ +>Illumina_Paired_End_DNA_Adapters1_1 +GAT-CGGAAGAGCGGTTCAGCAGGAATGCCGAG +>FC12044_91407_8_200_553_135 +--TACGGAGCCGCGG----GCGGGAAAGGCG-- +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTAC----------------ACGACGCTCTTCCGATCT +>FC12044_91407_8_200_553_135 +------------TACGGAGCCGCGGGCGGGAAAGGCG------------ +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCAC---CGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC +T +>FC12044_91407_8_200_553_135 +-----TACGGAG-CCGCGGGCGGGA------------------AAGGCG----------- +- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGC-TGAACCGCTCTTCCGAT +CT +>FC12044_91407_8_200_553_135 +----------------TACG-GA---GCCGCGG-------GCGGGAAAGGCG-------- +-- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTAC----------------ACGACGCTCTTCCGATCT +>FC12044_91407_8_200_553_135 +------------TACGGAGCCGCGGGCGGGAAAGGCG------------ +>Illumina_Paired_End_DNA_sequencing_primer_2 +----------CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGG--GCGG----------GAAAGGCG---------- +>Illumina_DpnII_Gex_Adapters1_1 +----GATCGTCGGACTGTAGAACTCTGAAC---- +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGG---------GAAAGGCG +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTAC--AGTCCGAC-------------- +>FC12044_91407_8_200_553_135 +---------------TACGGAG-CCG-CGGGCGGGAAAGGCG +>Illumina_DpnII_Gex_Adapters2_1 +----------CAAGCAG--AAGACGGCATACGA +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGGGAAAGGCG-------- +>Illumina_DpnII_Gex_Adapters2_2 +-TCGTATGCCGTCTTCTGCTTG----------- +>FC12044_91407_8_200_553_135 +TACGGA-GCC-------GCGGGCGGGAAAGGCG +>Illumina_DpnII_Gex_PCR_Primer_1 +----------CAAGCAG--AAGACGGCATACGA +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGGGAAAGGCG-------- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_553_135 +-----TACGGAG-CCGCGGGCGGG-----------AAAGGCG-- +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTAC--AGTCCGACGATC---------- +>FC12044_91407_8_200_553_135 +-----------------TACGGAG-CCG-CGGGCGGGAAAGGCG +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTGTAGAACTCTGAAC----------------- +>FC12044_91407_8_200_553_135 +--------TA-----CGGAGCCGCGGGCGGGAAAGGCG +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTAC--AGTCC--------GACATG-- +>FC12044_91407_8_200_553_135 +---------------TACGGAG-CCGCGGGCGGGAAAGGCG +>Illumina_NlaIII_Gex_Adapters2_1 +----------CAAGCAG--AAGACGGCATACGANN +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGGGAAAGGCG---------- +>Illumina_NlaIII_Gex_Adapters2_2 +-TCGTATGCCGTCTTCTGCTTG----------- +>FC12044_91407_8_200_553_135 +TACGGA-GCC-------GCGGGCGGGAAAGGCG +>Illumina_NlaIII_Gex_PCR_Primer_1 +----------CAAGCAG--AAGACGGCATACGA +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGGGAAAGGCG-------- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_553_135 +-----TACGGAG-CCGCGGGCGGG-----------AAAGGCG-- +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTAC--AGTCC--------GACATG-- +>FC12044_91407_8_200_553_135 +------------------TACGGAG-CCGCGGGCGGGAAAGGCG +>Illumina_Small_RNA_RT_Primer +----------CAAGCAG--AAGACGGCATACGA +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGGGAAAGGCG-------- +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTAC--AGTCCGACGATC---------- +>FC12044_91407_8_200_553_135 +-----------TACGGAG-CCG-CGGGCGGGAAAGGCG +>Illumina_Small_RNA_3p_Adapter +----------------------TCGTATGCCGTCTTCTGCTTGT +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGGGAAAGGCG------------------- +>Illumina_Small_RNA_PCR_Primer_1 +----------CAAGCAG--AAGACGGCATACGA +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGGGAAAGGCG-------- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_553_135 +-----TACGGAG-CCGCGGGCGGG-----------AAAGGCG-- +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTAC--AGTCCGACGATC---------- +>FC12044_91407_8_200_553_135 +-----------------TACGGAG-CCG-CGGGCGGGAAAGGCG +>Illumina_Genomici_DNA_Adapters1_1 +--------GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTC--AAGCGAT--TATCCT------------ +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCT-ACACGACGCTCTTC---CGAT----CT +>FC12044_91407_8_200_139_74 +----------CCTCCCAGG------TTCAAGCGATTATCCT +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_139_74 +-------------CCTCCCAGGTTCAAGCGATTATCCT-------------------- +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGCTC-----TTC---CGAT----CT +>FC12044_91407_8_200_139_74 +---------------------CCTCCCAGGTTCAAGCGATTATCCT +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCT-ACACGACGCTCTTC---CGAT----CT +>FC12044_91407_8_200_139_74 +----------CCTCCCAGG------TTCAAGCGATTATCCT +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGC------GGTTCAGCAGGAATGCCGAG------ +>FC12044_91407_8_200_139_74 +-----------CCTCCCAGGTTCA--AG------CGATTATCCT +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCT-ACACGACGCTCTTC---CGAT----CT +>FC12044_91407_8_200_139_74 +----------CCTCCCAGG------TTCAAGCGATTATCCT +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_139_74 +-------------CCTCCCAGGTTCAAGCGATTATCCT-------------------- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCT-----GCTGAACCGCTCTTC +CGATCT +>FC12044_91407_8_200_139_74 +-------------------------------------CCTCCCAGGTTCAAGCGATTATC +CT---- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCT-ACACGACGCTCTTC---CGAT----CT +>FC12044_91407_8_200_139_74 +----------CCTCCCAGG------TTCAAGCGATTATCCT +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCT-----GCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_139_74 +-------------CCTCCCAGGTTCAAGCGATTATCCT---- +>Illumina_DpnII_Gex_Adapters1_1 +----------------GATCGTCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCAAGCGATTATC---CT-------------- +>Illumina_DpnII_Gex_Adapters1_2 +----ACAGGTTCAGAG------TTCTACAGTCCGAC +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCA-AGCGATTATCCT---------- +>Illumina_DpnII_Gex_Adapters2_1 +-----------CAAGCAGAAGACGGCATACGA------- +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCAAG--------------CGATTATCCT +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTC--------------TTCTGCTTG +>FC12044_91407_8_200_139_74 +--------CCTCCCAGGTTCAAGCGATTATCCT-- +>Illumina_DpnII_Gex_PCR_Primer_1 +-----------CAAGCAGAAGACGGCATACGA------- +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCAAG--------------CGATTATCCT +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA------- +>FC12044_91407_8_200_139_74 +-------------CCTCC--CAGGTTCA-AG----------CGATTATCCT +>Illumina_DpnII_Gex_sequencing_primer +----CGACAGGTTCAGAGTTCTACAGTCCGACGATC-- +>FC12044_91407_8_200_139_74 +CCTCC--CAGGTTCA-AG----------CGATTATCCT +>Illumina_NlaIII_Gex_Adapters1_1 +---------------------TCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCAAGCGATTATC---CT-------------- +>Illumina_NlaIII_Gex_Adapters1_2 +----ACAGGTTCAGAG------TTCTACAGTCCGACATG +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCA-AGCGATTATCCT------------- +>Illumina_NlaIII_Gex_Adapters2_1 +-----------CAAGCAGAAGACGGCATACGANN----- +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCAAG--------------CGATTATCCT +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTC--------------TTCTGCTTG +>FC12044_91407_8_200_139_74 +--------CCTCCCAGGTTCAAGCGATTATCCT-- +>Illumina_NlaIII_Gex_PCR_Primer_1 +-----------CAAGCAGAAGACGGCATACGA------- +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCAAG--------------CGATTATCCT +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA------- +>FC12044_91407_8_200_139_74 +-------------CCTCC--CAGGTTCA-AG----------CGATTATCCT +>Illumina_NlaIII_Gex_sequencing_primer +---CCGACAGGTTCAGAG------TTCTACAGTCCGACATG +>FC12044_91407_8_200_139_74 +CCTCC--CAGGTTCA-AGCGATTATCCT------------- +>Illumina_Small_RNA_RT_Primer +-----------CAAGCAGAAGACGGCATACGA------- +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCAAG--------------CGATTATCCT +>Illumina_Small_RNA_5p_Adapter +--------GTTCAGAGTTCTACAGTCCGACGATC-- +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCA-AG----------CGATTATCCT +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTC--------------TTCTGCTTGT +>FC12044_91407_8_200_139_74 +--------CCTCCCAGGTTCAAGCGATTATCCT--- +>Illumina_Small_RNA_PCR_Primer_1 +-----------CAAGCAGAAGACGGCATACGA------- +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCAAG--------------CGATTATCCT +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA------- +>FC12044_91407_8_200_139_74 +-------------CCTCC--CAGGTTCA-AG----------CGATTATCCT +>Illumina_Small_RNA_sequencing_primer +----CGACAGGTTCAGAGTTCTACAGTCCGACGATC-- +>FC12044_91407_8_200_139_74 +CCTCC--CAGGTTCA-AG----------CGATTATCCT +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGT-ATGCCGTCTTCTGCTTG------- +>FC12044_91407_8_200_108_33 +--------------GTCATGGCGGC--CCGCGCGGGGAGCG +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTT------CCGATCT------- +>FC12044_91407_8_200_108_33 +---------------------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACC---------ACCGAGATCTACACTCTTTCCCTACACGACGCTCTT +CCGATCT +>FC12044_91407_8_200_108_33 +---GTCATGGCGGCCCGCGCGGGGAGCG-------------------------------- +------- +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAG--AAGACGGCATAC-----GAGCTCTTCCGATCT +>FC12044_91407_8_200_108_33 +------GTCATGGCGGCCCGCGCGGGGAGCG---------- +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGACGCTCTT------CCGATCT------- +>FC12044_91407_8_200_108_33 +---------------------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_Paired_End_DNA_Adapters1_1 +-------------GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG +>FC12044_91407_8_200_108_33 +GTCATGGCGGCCCGCGCGG-GGAGCG------------------- +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTT------CCGATCT------- +>FC12044_91407_8_200_108_33 +---------------------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACC---------ACCGAGATCTACACTCTTTCCCTACACGACGCTCTT +CCGATCT +>FC12044_91407_8_200_108_33 +---GTCATGGCGGCCCGCGCGGGGAGCG-------------------------------- +------- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGC---TGAACCGCTCTTCCG +ATCT +>FC12044_91407_8_200_108_33 +------------GTCAT--------GG---CGG----CCCGCGCGGGGAGCG-------- +---- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGACGCTCTT------CCGATCT------- +>FC12044_91407_8_200_108_33 +---------------------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGC---TGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_108_33 +--GTCATGGC-GGCCCGCGCGGGGAGCG------------ +>Illumina_DpnII_Gex_Adapters1_1 +--------------------GATCGTCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_108_33 +GTCATGGCGGCCCGCGCGGGGAGCG--------------------- +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAGTC----CGAC-------------- +>FC12044_91407_8_200_108_33 +-------------------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_DpnII_Gex_Adapters2_1 +CAAGCAG--AAGACGGCATACGA-------- +>FC12044_91407_8_200_108_33 +------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_DpnII_Gex_Adapters2_2 +TCGT-ATGCCGTCTTCTGCTTG------- +>FC12044_91407_8_200_108_33 +--GTCATGGCGGC--CCGCGCGGGGAGCG +>Illumina_DpnII_Gex_PCR_Primer_1 +CAAGCAG--AAGACGGCATACGA-------- +>FC12044_91407_8_200_108_33 +------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_108_33 +---GTCATGGCGGCCCGCG-CGGG----GAG----------CG- +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTACAGTC-------------CGACGATC- +>FC12044_91407_8_200_108_33 +---------------------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_NlaIII_Gex_Adapters1_1 +----------------------TCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_108_33 +GTCATGGCGGCCCGCGCGGGGAGCG------------------ +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAGTCCGACATG------------------- +>FC12044_91407_8_200_108_33 +-----------------------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_NlaIII_Gex_Adapters2_1 +CAAGCAG--AAGACGGCATACGANN------ +>FC12044_91407_8_200_108_33 +------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_NlaIII_Gex_Adapters2_2 +TCGT-ATGCCGTCTTCTGCTTG------- +>FC12044_91407_8_200_108_33 +--GTCATGGCGGC--CCGCGCGGGGAGCG +>Illumina_NlaIII_Gex_PCR_Primer_1 +CAAGCAG--AAGACGGCATACGA-------- +>FC12044_91407_8_200_108_33 +------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_108_33 +---GTCATGGCGGCCCGCG-CGGG----GAG----------CG- +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTACAGTCCGACATG------------------- +>FC12044_91407_8_200_108_33 +--------------------------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_Small_RNA_RT_Primer +CAAGCAG--AAGACGGCATACGA-------- +>FC12044_91407_8_200_108_33 +------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTACAGTC-------------CGACGATC- +>FC12044_91407_8_200_108_33 +---------------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_Small_RNA_3p_Adapter +TCGT-ATGCCGTCTTCTGCTTGT------ +>FC12044_91407_8_200_108_33 +--GTCATGGCGGC--CCGCGCGGGGAGCG +>Illumina_Small_RNA_PCR_Primer_1 +CAAGCAG--AAGACGGCATACGA-------- +>FC12044_91407_8_200_108_33 +------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_108_33 +---GTCATGGCGGCCCGCG-CGGG----GAG----------CG- +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTACAGTC-------------CGACGATC- +>FC12044_91407_8_200_108_33 +---------------------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_Genomici_DNA_Adapters1_1 +-------GATC----GGAAGAGCTCGTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAAAGAAGAG-TCG------------------ +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGA-----CGCTCTTCCGATCT +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTA-AAGAAGAGTCG------------ +>Illumina_Genomic_DNA_PCR_Primers1_1 +-------------AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGC +TCTTCCGATCT +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAAAGAAGAGTCG----------------------------------- +----------- +>Illumina_Genomic_DNA_PCR_Primers1_2 +------------CAAGCAGAAGA--CGGCATACGAGCTCTTCCGATCT +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAA--AGAAGAGTCG--------------------- +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGA-----CGCTCTTCCGATCT +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTA-AAGAAGAGTCG------------ +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAG-CGGTTCAGCAGGAATGCCGAG--- +>FC12044_91407_8_200_980_965 +-------ACAGTGGGTTCTTAAAGAA----GAGTCG +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGA-----CGCTCTTCCGATCT +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTA-AAGAAGAGTCG------------ +>Illumina_Paired_End_DNA_PCR_Primers1_1 +-------------AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGC +TCTTCCGATCT +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAAAGAAGAGTCG----------------------------------- +----------- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +------------CAAGCAGAAGA--CGGCATACGAGATCGGTCTCGGCATTCCTGCTGAA +CCGCTCTTCCGATCT +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAA--AGAAGAGTCG--------------------------------- +--------------- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGA-----CGCTCTTCCGATCT +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTA-AAGAAGAGTCG------------ +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT------------- +>FC12044_91407_8_200_980_965 +---------------------ACAG----TGGGTTCTTAAAGAAGAGTCG +>Illumina_DpnII_Gex_Adapters1_1 +-------------------GATCGTCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAAAGAAGA--GTCG------------------ +>Illumina_DpnII_Gex_Adapters1_2 +ACA---GGTTC----AGAGTTCTACAGTCCGAC +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAAAGA-----AGAGTCG--- +>Illumina_DpnII_Gex_Adapters2_1 +------------CAAGCAGAAGA--CGGCATACGA +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAA--AGAAGAGTCG-------- +>Illumina_DpnII_Gex_Adapters2_2 +----------------------TCGTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAAAGAAGAGTCG------------------ +>Illumina_DpnII_Gex_PCR_Primer_1 +------------CAAGCAGAAGA--CGGCATACGA +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAA--AGAAGAGTCG-------- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACA---GGTTC----AGAGTTCTACAGTCCGA +>FC12044_91407_8_200_980_965 +-------------------ACAGTGGGTTCTTAAAGA-----AGAGTCG-- +>Illumina_DpnII_Gex_sequencing_primer +CGACA---GGTTC----AGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_980_965 +--ACAGTGGGTTCTTAAAGA-----AGAGTCG------- +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTGTAGAACTCTGAAC--------- +>FC12044_91407_8_200_980_965 +----ACAGT-GGGTTCTTAAAGAAGAGTCG +>Illumina_NlaIII_Gex_Adapters1_2 +ACA---GGTTC----AGAGTTCTACAGTCCGACATG +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAAAGA-----AGAGTCG------ +>Illumina_NlaIII_Gex_Adapters2_1 +------------CAAGCAGAAGA--CGGCATACGANN +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAA--AGAAGAGTCG---------- +>Illumina_NlaIII_Gex_Adapters2_2 +----------------------TCGTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAAAGAAGAGTCG------------------ +>Illumina_NlaIII_Gex_PCR_Primer_1 +------------CAAGCAGAAGA--CGGCATACGA +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAA--AGAAGAGTCG-------- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACA---GGTTC----AGAGTTCTACAGTCCGA +>FC12044_91407_8_200_980_965 +-------------------ACAGTGGGTTCTTAAAGA-----AGAGTCG-- +>Illumina_NlaIII_Gex_sequencing_primer +CCGACA---GGTTC----AGAGTTCTACAGTCCGACATG +>FC12044_91407_8_200_980_965 +---ACAGTGGGTTCTTAAAGA-----AGAGTCG------ +>Illumina_Small_RNA_RT_Primer +------------CAAGCAGAAGA--CGGCATACGA +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAA--AGAAGAGTCG-------- +>Illumina_Small_RNA_5p_Adapter +---GTTCAGAGTTCT------ACAGTCCGACGATC +>FC12044_91407_8_200_980_965 +ACAGT---GGGTTCTTAAAGAAGAGTCG------- +>Illumina_Small_RNA_3p_Adapter +----------------------TCGTATGCCGTCTTCTGCTTGT +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAAAGAAGAGTCG------------------- +>Illumina_Small_RNA_PCR_Primer_1 +------------CAAGCAGAAGA--CGGCATACGA +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAA--AGAAGAGTCG-------- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACA---GGTTC----AGAGTTCTACAGTCCGA +>FC12044_91407_8_200_980_965 +-------------------ACAGTGGGTTCTTAAAGA-----AGAGTCG-- +>Illumina_Small_RNA_sequencing_primer +CGACA---GGTTC----AGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_980_965 +--ACAGTGGGTTCTTAAAGA-----AGAGTCG------- +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGTATGC-CGTCTTCTGCTTG- +>FC12044_91407_8_200_981_857 +-AACG--AGGG-------GCGCGACTTGACCTTGG +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGA----CGC-TCTTCCGATCT--- +>FC12044_91407_8_200_981_857 +--------------AACGAGGGGCGCGACTT--GACCTTGG +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGA----CGC-TCTTCCGA +TCT--- +>FC12044_91407_8_200_981_857 +---------------------------------------AACGAGGGGCGCGACTT--GA +CCTTGG +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAA-GACGGCATACGAGC-TCTTCCGATCT--- +>FC12044_91407_8_200_981_857 +-------AACGAGGG-----GCGCGACTT--GACCTTGG +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGA----CGC-TCTTCCGATCT--- +>FC12044_91407_8_200_981_857 +--------------AACGAGGGGCGCGACTT--GACCTTGG +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG------------------- +>FC12044_91407_8_200_981_857 +-----------------------AA---CGAGGGGCGCGACTTGACCTTGG +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGA----CGC-TCTTCCGATCT--- +>FC12044_91407_8_200_981_857 +--------------AACGAGGGGCGCGACTT--GACCTTGG +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGA----CGC-TCTTCCGA +TCT--- +>FC12044_91407_8_200_981_857 +---------------------------------------AACGAGGGGCGCGACTT--GA +CCTTGG +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATT--CCTGCTGAACCGCTCTTCCGA +TCT +>FC12044_91407_8_200_981_857 +----------------AACGAG---GGGCGCGAC-TTGACCT--TGG------------- +--- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGA----CGC-TCTTCCGATCT--- +>FC12044_91407_8_200_981_857 +--------------AACGAGGGGCGCGACTT--GACCTTGG +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGCTGAAC------CGC-TCTTCCGATCT--- +>FC12044_91407_8_200_981_857 +--------------------AACGAGGGGCGCGACTT--GACCTTGG +>Illumina_DpnII_Gex_Adapters1_1 +---GATCGTCG-GACTGTAGAACTCTGAAC---- +>FC12044_91407_8_200_981_857 +AACGAGGGGCGCGACT---------TGACCTTGG +>Illumina_DpnII_Gex_Adapters1_2 +-AC-AGGTTC-AGAGTTCTACAGTCCGAC----- +>FC12044_91407_8_200_981_857 +AACGAGGGGCGCGACTT---------GACCTTGG +>Illumina_DpnII_Gex_Adapters2_1 +CAAGCAGAAGACGGCATACGA-------------------- +>FC12044_91407_8_200_981_857 +----------------AACGAGGGGCGCGACTTGACCTTGG +>Illumina_DpnII_Gex_Adapters2_2 +--------TCGTATGCCGTCTTCTGCTTG- +>FC12044_91407_8_200_981_857 +AACGAGGGGCG-----CGACTTGACCTTGG +>Illumina_DpnII_Gex_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA-------------------- +>FC12044_91407_8_200_981_857 +----------------AACGAGGGGCGCGACTTGACCTTGG +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGAC-------AGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_981_857 +AACGA-GGGGCG-----CGACTTGACCTTGG-------------------- +>Illumina_DpnII_Gex_sequencing_primer +--CGACAGGTTCAGAGTTCTACAGTCCGACGATC---- +>FC12044_91407_8_200_981_857 +AACGAGGGGCGC-GACTT------------GACCTTGG +>Illumina_NlaIII_Gex_Adapters1_1 +--------TCG-GACTGTAGAACTCTGAAC---- +>FC12044_91407_8_200_981_857 +AACGAGGGGCGCGACT---------TGACCTTGG +>Illumina_NlaIII_Gex_Adapters1_2 +-AC-AGGTTCAGAGTTCTACAGTC-CGACATG------- +>FC12044_91407_8_200_981_857 +AACGAGG--------------GGCGCGACTTGACCTTGG +>Illumina_NlaIII_Gex_Adapters2_1 +CAAGCAGAAGACGGCATACGANN------------------ +>FC12044_91407_8_200_981_857 +----------------AACGAGGGGCGCGACTTGACCTTGG +>Illumina_NlaIII_Gex_Adapters2_2 +--------TCGTATGCCGTCTTCTGCTTG- +>FC12044_91407_8_200_981_857 +AACGAGGGGCG-----CGACTTGACCTTGG +>Illumina_NlaIII_Gex_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA-------------------- +>FC12044_91407_8_200_981_857 +----------------AACGAGGGGCGCGACTTGACCTTGG +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGAC-------AGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_981_857 +AACGA-GGGGCG-----CGACTTGACCTTGG-------------------- +>Illumina_NlaIII_Gex_sequencing_primer +CCGAC-AGGTTCAGAGTTCTACAGTC-CGACATG------- +>FC12044_91407_8_200_981_857 +--AACGAGG--------------GGCGCGACTTGACCTTGG +>Illumina_Small_RNA_RT_Primer +CAAGCAGAAGACGGCATACGA-------------------- +>FC12044_91407_8_200_981_857 +----------------AACGAGGGGCGCGACTTGACCTTGG +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTACA-------GTCCGAC--GATC---- +>FC12044_91407_8_200_981_857 +--------------AACGAGGGGCGCGACTTGACCTTGG +>Illumina_Small_RNA_3p_Adapter +--------TCGTATGCCGTCTTCTGCTTGT +>FC12044_91407_8_200_981_857 +AACGAGGGGCG-----CGACTTGACCTTGG +>Illumina_Small_RNA_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA-------------------- +>FC12044_91407_8_200_981_857 +----------------AACGAGGGGCGCGACTTGACCTTGG +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGAC-------AGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_981_857 +AACGA-GGGGCG-----CGACTTGACCTTGG-------------------- +>Illumina_Small_RNA_sequencing_primer +--CGACAGGTTCAGAGTTCTACAGTCCGACGATC---- +>FC12044_91407_8_200_981_857 +AACGAGGGGCGC-GACTT------------GACCTTGG +>Illumina_Genomici_DNA_Adapters1_1 +-------------GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG--- +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAGGA-------AGC-----------------CTTGGAC +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTA---CACGACGCTCTT---CCGATCT +>FC12044_91407_8_200_8_865 +------TTTCCC-ACCCCAGGAAGC-CTTGGAC------ +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTA---CACGACGCTCTT---CCG +ATCT +>FC12044_91407_8_200_8_865 +-------------------------------TTTCCC-ACCCCAGGAAGC-CTTGGAC-- +---- +>Illumina_Genomic_DNA_PCR_Primers1_2 +---------CAAGCAGAAGAC--GGCATACGAGCTCTTCCGATCT +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAG--GAAGCCTTGG---AC--------------- +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTA---CACGACGCTCTT---CCGATCT +>FC12044_91407_8_200_8_865 +------TTTCCC-ACCCCAGGAAGC-CTTGGAC------ +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTT-----CAGCAGGAATGCCGAG--- +>FC12044_91407_8_200_8_865 +--------------TTTCCCACCCCAGGAA-GCCTTGGAC +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTA---CACGACGCTCTT---CCGATCT +>FC12044_91407_8_200_8_865 +------TTTCCC-ACCCCAGGAAGC-CTTGGAC------ +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTA---CACGACGCTCTT---CCG +ATCT +>FC12044_91407_8_200_8_865 +-------------------------------TTTCCC-ACCCCAGGAAGC-CTTGGAC-- +---- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +---------CAAGCAGAAGAC--GGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCG +CTCTTCCGATCT +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAG--GAAGCCTTGG---AC------------------------------ +------------ +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTA---CACGACGCTCTT---CCGATCT +>FC12044_91407_8_200_8_865 +------TTTCCC-ACCCCAGGAAGC-CTTGGAC------ +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTC--------GGCATTCCTGCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_8_865 +---TTTCCCACCCCAGG-AAGCCT--TGGAC-------------- +>Illumina_DpnII_Gex_Adapters1_1 +--------------GATCGTCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAGGAAGCCTTGGAC--------------- +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAGTCCGAC----------------- +>FC12044_91407_8_200_8_865 +------------TT------TCCCACCCCAGGAAGCCTTGGAC +>Illumina_DpnII_Gex_Adapters2_1 +---------CAAGCAGAAGAC--GGCATACGA +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAG--GAAGCCTTGG---AC-- +>Illumina_DpnII_Gex_Adapters2_2 +--TC--------GTATGCCGTCTTCTGCTTG--- +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAGGAAGC---------CTTGGAC +>Illumina_DpnII_Gex_PCR_Primer_1 +---------CAAGCAGAAGAC--GGCATACGA +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAG--GAAGCCTTGG---AC-- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_8_865 +----TTTC-----CCACC-CCAGG--AAGCCTTGGAC------- +>Illumina_DpnII_Gex_sequencing_primer +-------CGACAGGTTCAGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAGG--AAGCCTTGGAC------------ +>Illumina_NlaIII_Gex_Adapters1_1 +-------------------TCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAGGAAGCCTTGGAC--------------- +>Illumina_NlaIII_Gex_Adapters1_2 +---------ACAGGTTCAGAGTTCTACAGTCCGACATG +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAGG--AAGCCTTGGAC----------- +>Illumina_NlaIII_Gex_Adapters2_1 +---------CAAGCAGAAGAC--GGCATACGANN +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAG--GAAGCCTTGG---AC---- +>Illumina_NlaIII_Gex_Adapters2_2 +--TC--------GTATGCCGTCTTCTGCTTG--- +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAGGAAGC---------CTTGGAC +>Illumina_NlaIII_Gex_PCR_Primer_1 +---------CAAGCAGAAGAC--GGCATACGA +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAG--GAAGCCTTGG---AC-- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_8_865 +----TTTC-----CCACC-CCAGG--AAGCCTTGGAC------- +>Illumina_NlaIII_Gex_sequencing_primer +---CCGA---CAGGTTCAGAGTTCTACAGTCCGACATG +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAGG--AAGCCTTGGAC----------- +>Illumina_Small_RNA_RT_Primer +---------CAAGCAGAAGAC--GGCATACGA +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAG--GAAGCCTTGG---AC-- +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTACAGTCCGACGATC---------------- +>FC12044_91407_8_200_8_865 +--------TT------TCCCAC---CCCAGGAAGCCTTGGAC +>Illumina_Small_RNA_3p_Adapter +--TC--------GTATGCCGTCTTCTGCTTGT-- +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAGGAAGC---------CTTGGAC +>Illumina_Small_RNA_PCR_Primer_1 +---------CAAGCAGAAGAC--GGCATACGA +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAG--GAAGCCTTGG---AC-- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_8_865 +----TTTC-----CCACC-CCAGG--AAGCCTTGGAC------- +>Illumina_Small_RNA_sequencing_primer +-------CGACAGGTTCAGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAGG--AAGCCTTGGAC------------ +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGC--TCGTATGCC--GTCTTCTGCTTG +>FC12044_91407_8_200_292_484 +--TC-----AGCCTCCG--TGCCCAGCCCACTCC--- +>Illumina_Genomic_DNA_Adapters1_2 +--ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_292_484 +TCAGCCTCCGTGCCCAGCCCAC-----TCC----- +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_292_484 +----------------------TC-AGCCTCCGTGCCCAGCCCAC-----TCC----- +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT-- +>FC12044_91407_8_200_292_484 +----------TCAGCCTCCGTGCCCAGCCCA-CTCC +>Illumina_Genomic_DNA_sequencing_primer +--ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_292_484 +TCAGCCTCCGTGCCCAGCCCAC-----TCC----- +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG-------- +>FC12044_91407_8_200_292_484 +---------------TCAGCCTCCGTGCCCAGCCCACTCC +>Illumina_Paired_End_DNA_Adapters1_2 +--ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_292_484 +TCAGCCTCCGTGCCCAGCCCAC-----TCC----- +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_292_484 +----------------------TC-AGCCTCCGTGCCCAGCCCAC-----TCC----- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATC +T +>FC12044_91407_8_200_292_484 +-----------------------------TCAGCCTCCGTGCCCAGCC-CACTCC----- +- +>Illumina_Paired_End_DNA_sequencing_primer_1 +--ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_292_484 +TCAGCCTCCGTGCCCAGCCCAC-----TCC----- +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_292_484 +-----TCAGCCTCCGTGCCCAGCC-CACTCC------ +>Illumina_DpnII_Gex_Adapters1_1 +GATCGTCGGACT--GT----AG--AACTCTGAAC +>FC12044_91407_8_200_292_484 +-----TCAGCCTCCGTGCCCAGCCCACTCC---- +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAG----AGTTCTACAGTCCGAC--- +>FC12044_91407_8_200_292_484 +------TCAGCCTCCGTGC-CCAG-CCCACTCC +>Illumina_DpnII_Gex_Adapters2_1 +CAAGCAGAAGACGGCATACGA-------------- +>FC12044_91407_8_200_292_484 +----------TCAGCCTCCGTGCCCAGCCCACTCC +>Illumina_DpnII_Gex_Adapters2_2 +-------TCGTATGCC--GTCTTCTGCTTG +>FC12044_91407_8_200_292_484 +TCAGCCTCCG--TGCCCAGCCCACTCC--- +>Illumina_DpnII_Gex_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA-------------- +>FC12044_91407_8_200_292_484 +----------TCAGCCTCCGTGCCCAGCCCACTCC +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAG----AGTTCTACAG-----TCCGA +>FC12044_91407_8_200_292_484 +-------------------------TCAGCCTCCGTGC-CCAGCCCACTCC-- +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAG----AGTTCTACAGTCCGACGATC- +>FC12044_91407_8_200_292_484 +--------TCAGCCTCCGTGC-CCAG-CCCAC--TCC +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACT--GT----AG--AACTCTGAAC +>FC12044_91407_8_200_292_484 +TCAGCCTCCGTGCCCAGCCCACTCC---- +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAG--TCCGACATG------------- +>FC12044_91407_8_200_292_484 +----------------TCAGCCTCCG---TGCCCAGCCCACTCC +>Illumina_NlaIII_Gex_Adapters2_1 +CAAGCAGAAGACGGCATACGANN------------ +>FC12044_91407_8_200_292_484 +----------TCAGCCTCCGTGCCCAGCCCACTCC +>Illumina_NlaIII_Gex_Adapters2_2 +-------TCGTATGCC--GTCTTCTGCTTG +>FC12044_91407_8_200_292_484 +TCAGCCTCCG--TGCCCAGCCCACTCC--- +>Illumina_NlaIII_Gex_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA-------------- +>FC12044_91407_8_200_292_484 +----------TCAGCCTCCGTGCCCAGCCCACTCC +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAG----AGTTCTACAG-----TCCGA +>FC12044_91407_8_200_292_484 +-------------------------TCAGCCTCCGTGC-CCAGCCCACTCC-- +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTACAG--TCCGACATG------------- +>FC12044_91407_8_200_292_484 +-------------------TCAGCCTCCG---TGCCCAGCCCACTCC +>Illumina_Small_RNA_RT_Primer +CAAGCAGAAGACGGCATACGA-------------- +>FC12044_91407_8_200_292_484 +----------TCAGCCTCCGTGCCCAGCCCACTCC +>Illumina_Small_RNA_5p_Adapter +GTTCAG----AGTTCTACAGTCCGACGATC- +>FC12044_91407_8_200_292_484 +--TCAGCCTCCGTGC-CCAG-CCCAC--TCC +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTCTTCTGCTT--GT-------------- +>FC12044_91407_8_200_292_484 +-------------TCAGCCTCCGTGCCCAGCCCACTCC +>Illumina_Small_RNA_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA-------------- +>FC12044_91407_8_200_292_484 +----------TCAGCCTCCGTGCCCAGCCCACTCC +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAG----AGTTCTACAG-----TCCGA +>FC12044_91407_8_200_292_484 +-------------------------TCAGCCTCCGTGC-CCAGCCCACTCC-- +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAG----AGTTCTACAGTCCGACGATC- +>FC12044_91407_8_200_292_484 +--------TCAGCCTCCGTGC-CCAG-CCCAC--TCC +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGTATGCCG------TCTTCTGCTTG +>FC12044_91407_8_200_675_16 +-CTCGGGAG-GCT--GAGGCAGGGGGGTT---------- +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTTCCGATCT----------------------- +>FC12044_91407_8_200_675_16 +-------------------------------CTCGGGAGGCTGAGGCAGGGGGGTT +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-- +--------------------- +>FC12044_91407_8_200_675_16 +--------------------------------------------------------CTCG +GGAGGCTGAGGCAGGGGGGTT +>Illumina_Genomic_DNA_PCR_Primers1_2 +-----CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGA----GGCAGGGGGGTT---------- +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGACGCTCTTCCGATCT----------------------- +>FC12044_91407_8_200_675_16 +-------------------------------CTCGGGAGGCTGAGGCAGGGGGGTT +>Illumina_Paired_End_DNA_Adapters1_1 +-----------GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGA--GGCAGGGGGGTT---------------- +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTTCCGATCT----------------------- +>FC12044_91407_8_200_675_16 +-------------------------------CTCGGGAGGCTGAGGCAGGGGGGTT +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-- +--------------------- +>FC12044_91407_8_200_675_16 +--------------------------------------------------------CTCG +GGAGGCTGAGGCAGGGGGGTT +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACC------GCTCTT +CCGATCT +>FC12044_91407_8_200_675_16 +----------------------------CTCGGGA----GGCTGAGGCAGGGGGGTT--- +------- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGACGCTCTTCCGATCT----------------------- +>FC12044_91407_8_200_675_16 +-------------------------------CTCGGGAGGCTGAGGCAGGGGGGTT +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGCTGAACC------GCTCTTCCGATCT +>FC12044_91407_8_200_675_16 +----CTCGGGA----GGCTGAGGCAGGGGGGTT---------- +>Illumina_DpnII_Gex_Adapters1_1 +GATCGTCGGACTGTAGAACTCTGAAC----------------- +>FC12044_91407_8_200_675_16 +------------------CTCGGGAGGCTGAGGCAGGGGGGTT +>Illumina_DpnII_Gex_Adapters1_2 +--------------ACAGGTTCAGAGTTCTACAGTCCGAC +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGAGGCAGG---GGGGTT------------ +>Illumina_DpnII_Gex_Adapters2_1 +-----CAAGCAGAAGACGGCA-------TACGA +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGA----GGCAGGGGGGTT---- +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG--------------------- +>FC12044_91407_8_200_675_16 +-----------------CTCGGGAGGCTGAGGCAGGGGGGTT +>Illumina_DpnII_Gex_PCR_Primer_1 +-----CAAGCAGAAGACGGCA-------TACGA +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGA----GGCAGGGGGGTT---- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_675_16 +-------CTCGGGAGGCTGA-----GGCAGG---GGGGTT----------- +>Illumina_DpnII_Gex_sequencing_primer +--CGACAGGTTCA-------GAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGAGGCAGGGGGGTT---------------- +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTGTAGAACTCTGAAC----------------- +>FC12044_91407_8_200_675_16 +-------------CTCGGGAGGCTGAGGCAGGGGGGTT +>Illumina_NlaIII_Gex_Adapters1_2 +--------------ACAGGTTCAGAGTTCTACAGTCCGACATG +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGAGGCAGG---GGGGTT--------------- +>Illumina_NlaIII_Gex_Adapters2_1 +-----CAAGCAGAAGACGGCA-------TACGANN +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGA----GGCAGGGGGGTT------ +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG--------------------- +>FC12044_91407_8_200_675_16 +-----------------CTCGGGAGGCTGAGGCAGGGGGGTT +>Illumina_NlaIII_Gex_PCR_Primer_1 +-----CAAGCAGAAGACGGCA-------TACGA +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGA----GGCAGGGGGGTT---- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_675_16 +-------CTCGGGAGGCTGA-----GGCAGG---GGGGTT----------- +>Illumina_NlaIII_Gex_sequencing_primer +---------CCGA--CAGGTTCAGAGTTCTACAGTCCGACATG +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGAGGCAGG---GGGGTT--------------- +>Illumina_Small_RNA_RT_Primer +-----CAAGCAGAAGACGGCA-------TACGA +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGA----GGCAGGGGGGTT---- +>Illumina_Small_RNA_5p_Adapter +----------------------GTTCAGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGAGGCAGGGGGGTT----------------------- +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTCTTCTGCTTGT-------------------- +>FC12044_91407_8_200_675_16 +-----------------CTCGGGAGGCTGAGGCAGGGGGGTT +>Illumina_Small_RNA_PCR_Primer_1 +-----CAAGCAGAAGACGGCA-------TACGA +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGA----GGCAGGGGGGTT---- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_675_16 +-------CTCGGGAGGCTGA-----GGCAGG---GGGGTT----------- +>Illumina_Small_RNA_sequencing_primer +--CGACAGGTTCA-------GAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGAGGCAGGGGGGTT---------------- +>Illumina_Genomici_DNA_Adapters1_1 +---GATC-GGAA---GAGCTCGTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_285_136 +CCAAATCTTGAATTGTAGCTC-----CCCT---------- +>Illumina_Genomic_DNA_Adapters1_2 +--ACACTCTT----------TCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_285_136 +CCA-AATCTTGAATTGTAGCTCCC---------CT---------- +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACA-------CTCTTTCCCTACACGACGCTCTTCC +GATCT +>FC12044_91407_8_200_285_136 +-----------------CCAAATCTTGAATTGTAGCTC---CCCT--------------- +----- +>Illumina_Genomic_DNA_PCR_Primers1_2 +-CAAGCA----GAAGACGGCATACGAGCTCTTCCGATCT +>FC12044_91407_8_200_285_136 +CCAA--ATCTTGA-------ATTGTAGCTC--CC---CT +>Illumina_Genomic_DNA_sequencing_primer +--ACACTCTT----------TCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_285_136 +CCA-AATCTTGAATTGTAGCTCCC---------CT---------- +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG-------------------- +>FC12044_91407_8_200_285_136 +---------------------------CCAAATCTTGAATTGTAGCTCCCCT +>Illumina_Paired_End_DNA_Adapters1_2 +--ACACTCTT----------TCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_285_136 +CCA-AATCTTGAATTGTAGCTCCC---------CT---------- +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACA-------CTCTTTCCCTACACGACGCTCTTCC +GATCT +>FC12044_91407_8_200_285_136 +-----------------CCAAATCTTGAATTGTAGCTC---CCCT--------------- +----- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATC +T +>FC12044_91407_8_200_285_136 +------------------CCAAATC-----TTGAATTGTAGCT---CCCCT--------- +- +>Illumina_Paired_End_DNA_sequencing_primer_1 +--ACACTCTT----------TCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_285_136 +CCA-AATCTTGAATTGTAGCTCCC---------CT---------- +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCC---TGCTGAA--------CCGCTCTTCCGATCT +>FC12044_91407_8_200_285_136 +-------------CCAAATCTTGAATTGTAGCTCCCCT---------- +>Illumina_DpnII_Gex_Adapters1_1 +---GATCGTCGGACTGTAGAACT---CTGAAC +>FC12044_91407_8_200_285_136 +CCAAATC-TTGAATTGTAG--CTCCCCT---- +>Illumina_DpnII_Gex_Adapters1_2 +--ACAGGTTCAGAGTTCTACAG-TCCGAC-- +>FC12044_91407_8_200_285_136 +CCAAATCTT--GAATTGT--AGCTCC--CCT +>Illumina_DpnII_Gex_Adapters2_1 +CAAGCAGAAGACGGCATACGA---------------------- +>FC12044_91407_8_200_285_136 +------------------CCAAATCTTGAATTGTAGCTCCCCT +>Illumina_DpnII_Gex_Adapters2_2 +------------TCGTA--TGCCGTCTTCTGCTTG +>FC12044_91407_8_200_285_136 +CCAAATCTTGAATTGTAGCTCCCCT---------- +>Illumina_DpnII_Gex_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA---------------------- +>FC12044_91407_8_200_285_136 +------------------CCAAATCTTGAATTGTAGCTCCCCT +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA- +>FC12044_91407_8_200_285_136 +-----------------CCAAATCTT--GAATTGT--AGCTCCCCT +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTACAGTCCGACGATC---- +>FC12044_91407_8_200_285_136 +CCAAATCTT--GAATTGTA---------GCTCCCCT +>Illumina_NlaIII_Gex_Adapters1_1 +-------TCGGACTGTAGAACT---CTGAAC +>FC12044_91407_8_200_285_136 +CCAAATCTTGAATTGTAG--CTCCCCT---- +>Illumina_NlaIII_Gex_Adapters1_2 +--ACAGGTTCAGAGTTCTACAG-TCCGACATG +>FC12044_91407_8_200_285_136 +CCAAATCTT--GAATTGT--AGCTCC--CCT- +>Illumina_NlaIII_Gex_Adapters2_1 +CAAGCAGAAGACGGCATACGANN-------------------- +>FC12044_91407_8_200_285_136 +------------------CCAAATCTTGAATTGTAGCTCCCCT +>Illumina_NlaIII_Gex_Adapters2_2 +------------TCGTA--TGCCGTCTTCTGCTTG +>FC12044_91407_8_200_285_136 +CCAAATCTTGAATTGTAGCTCCCCT---------- +>Illumina_NlaIII_Gex_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA---------------------- +>FC12044_91407_8_200_285_136 +------------------CCAAATCTTGAATTGTAGCTCCCCT +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA- +>FC12044_91407_8_200_285_136 +-----------------CCAAATCTT--GAATTGT--AGCTCCCCT +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTACAG-TCCGACATG +>FC12044_91407_8_200_285_136 +-CCAAATCTT--GAATTGT--AGCTCC--CCT- +>Illumina_Small_RNA_RT_Primer +CAAGCAGAAGACGGCATACGA---------------------- +>FC12044_91407_8_200_285_136 +------------------CCAAATCTTGAATTGTAGCTCCCCT +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTACAGTCCGACGATC------------------ +>FC12044_91407_8_200_285_136 +-----------------CCAA--ATCTTGAATTGTAGCTCCCCT +>Illumina_Small_RNA_3p_Adapter +------------TCGTA--TGCCGTCTTCTGCTTGT +>FC12044_91407_8_200_285_136 +CCAAATCTTGAATTGTAGCTCCCCT----------- +>Illumina_Small_RNA_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA---------------------- +>FC12044_91407_8_200_285_136 +------------------CCAAATCTTGAATTGTAGCTCCCCT +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA- +>FC12044_91407_8_200_285_136 +-----------------CCAAATCTT--GAATTGT--AGCTCCCCT +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTACAGTCCGACGATC---- +>FC12044_91407_8_200_285_136 +CCAAATCTT--GAATTGTA---------GCTCCCCT diff --git a/tools/emboss_5/test-data/emboss_needleall_out.pair b/tools/emboss_5/test-data/emboss_needleall_out.pair new file mode 100644 index 00000000000..ff108845488 --- /dev/null +++ b/tools/emboss_5/test-data/emboss_needleall_out.pair @@ -0,0 +1,18267 @@ +######################################## +# Program: needleall +# Rundate: Mon 16 Dec 2024 17:13:24 +# Commandline: needleall +# -asequence /tmp/saskia/tmpet25av5g/files/e/e/8/dataset_ee891bb1-6c31-453a-8e30-69544c761887.dat +# -bsequence /tmp/saskia/tmpet25av5g/files/0/7/0/dataset_07021fba-c6f2-478f-8665-0e6832dba409.dat +# -outfile /tmp/saskia/tmpet25av5g/job_working_directory/000/9/outputs/dataset_b9c62224-ff77-442f-a21f-1e30d4ffb6fc.dat +# -gapopen 10.0 +# -gapextend 0.5 +# -brief yes +# -aformat3 pair +# -auto +# -datafile EPAM30 +# -endopen 13.37 +# -endextend 2.5 +# -minscore 1.0 +# Align_format: pair +# Report_file: /tmp/saskia/tmpet25av5g/job_working_directory/000/9/outputs/dataset_b9c62224-ff77-442f-a21f-1e30d4ffb6fc.dat +######################################## + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 15/37 (40.5%) +# Similarity: 15/37 (40.5%) +# Gaps: 16/37 (43.2%) +# Score: 61.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG---- 33 + |.| ||||| ||..|||..| .|| +FC12044_91407 1 GTT------AGCTC-----CCACCTTAAG-ATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Geno 1 ----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + .| |||| || |||..|||.| +FC12044_91407 1 GTTAGC------TCCC-AC--------CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 60 +# Identity: 17/60 (28.3%) +# Similarity: 17/60 (28.3%) +# Gaps: 37/60 (61.7%) +# Score: 68.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACC--GAGATCTACACTCTTTCCCTACACGACGCT 48 + |.|| ||..||||| .||||.| ||. +FC12044_91407 1 ---GTTA--GCTCCCACCTTAAGATGT------TTA-------------- 25 + +Illumina_Geno 49 CTTCCGATCT 58 + +FC12044_91407 26 ---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 11/47 (23.4%) +# Similarity: 11/47 (23.4%) +# Gaps: 35/47 (74.5%) +# Score: 49.0 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACG--AGCTCTTCCGATCT----------- 34 + | ||||| || |.|| +FC12044_91407 1 -------------------GTTAGCTC--CC-ACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Geno 1 ----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + .| |||| || |||..|||.| +FC12044_91407 1 GTTAGC------TCCC-AC--------CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 10/45 (22.2%) +# Similarity: 10/45 (22.2%) +# Gaps: 33/45 (73.3%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATGCC------GAG------- 32 + ||| |||. || .|| +FC12044_91407 1 -------------GTT-AGCT------CCCACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Pair 1 ----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + .| |||| || |||..|||.| +FC12044_91407 1 GTTAGC------TCCC-AC--------CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 60 +# Identity: 17/60 (28.3%) +# Similarity: 17/60 (28.3%) +# Gaps: 37/60 (61.7%) +# Score: 68.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACC--GAGATCTACACTCTTTCCCTACACGACGCT 48 + |.|| ||..||||| .||||.| ||. +FC12044_91407 1 ---GTTA--GCTCCCACCTTAAGATGT------TTA-------------- 25 + +Illumina_Pair 49 CTTCCGATCT 58 + +FC12044_91407 26 ---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 67 +# Identity: 15/67 (22.4%) +# Similarity: 15/67 (22.4%) +# Gaps: 48/67 (71.6%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGT---CTCGGCATTCCTGCTGAAC 47 + || ||| | +FC12044_91407 1 --------------------------GTTAGCTC---------------C 9 + +Illumina_Pair 48 CGCTCTTCCGATCT--- 61 + |.| |||..|||.| +FC12044_91407 10 CAC-CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Pair 1 ----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + .| |||| || |||..|||.| +FC12044_91407 1 GTTAGC------TCCC-AC--------CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 15/43 (34.9%) +# Similarity: 15/43 (34.9%) +# Gaps: 24/43 (55.8%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Pair 1 CGGT---CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT--- 37 + || ||| ||.| |||..|||.| +FC12044_91407 1 --GTTAGCTC---------------CCAC-CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 9/42 (21.4%) +# Similarity: 9/42 (21.4%) +# Gaps: 33/42 (78.6%) +# Score: 34.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAACTCTGAAC---------------- 26 + || ||| ||| | +FC12044_91407 1 ----GT-------TAG--CTC----CCACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 10/40 (25.0%) +# Similarity: 10/40 (25.0%) +# Gaps: 29/40 (72.5%) +# Score: 44.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACAG-TCCGAC------------- 26 + ||| || |||.|| +FC12044_91407 1 -----------GTT----AGCTCCCACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 30.5 +# +# +#======================================= + +Illumina_DpnI 1 --------CAAGCAGAAGACGGCATACGA------ 21 + | | || |.||.|| +FC12044_91407 1 GTTAGCTCC---C-----AC--CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 11/28 (39.3%) +# Similarity: 11/28 (39.3%) +# Gaps: 10/28 (35.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATG---CCGTCTTCTGCTTG---- 21 + ||..| ||..|||..| .|| +FC12044_91407 1 --GTTAGCTCCCACCTTAAG-ATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 30.5 +# +# +#======================================= + +Illumina_DpnI 1 --------CAAGCAGAAGACGGCATACGA------ 21 + | | || |.||.|| +FC12044_91407 1 GTTAGCTCC---C-----AC--CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 84.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGA-GTTCTACAGTCCGA 44 + |.|| ||..||||| ||.||| ||| || +FC12044_91407 1 ---GTTA--GCTCCCACC------TTAAGATGTT-TA-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 33/45 (73.3%) +# Score: 43.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACAG-TCCGACGATC------------ 32 + ||| || |||.|| | +FC12044_91407 1 -------------GTT----AGCTCCCAC---CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 7/37 (18.9%) +# Similarity: 7/37 (18.9%) +# Gaps: 28/37 (75.7%) +# Score: 31.5 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAACTCTGAAC---------------- 21 + ||. |.||| | +FC12044_91407 1 -------GTT-AGCTC----CCACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 26/40 (65.0%) +# Score: 51.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAG-TCCGAC------ATG---- 29 + ||| || |||.|| ||| +FC12044_91407 1 -----------GTT----AGCTCCCACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 9/36 (25.0%) +# Similarity: 9/36 (25.0%) +# Gaps: 24/36 (66.7%) +# Score: 26.5 +# +# +#======================================= + +Illumina_NlaI 1 ---------CAAGC----AGAAGACGGCATACGANN 23 + || | |||.|. .|| +FC12044_91407 1 GTTAGCTCCCA--CCTTAAGATGT----TTA----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 11/28 (39.3%) +# Similarity: 11/28 (39.3%) +# Gaps: 10/28 (35.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATG---CCGTCTTCTGCTTG---- 21 + ||..| ||..|||..| .|| +FC12044_91407 1 --GTTAGCTCCCACCTTAAG-ATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 30.5 +# +# +#======================================= + +Illumina_NlaI 1 --------CAAGCAGAAGACGGCATACGA------ 21 + | | || |.||.|| +FC12044_91407 1 GTTAGCTCC---C-----AC--CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 84.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGA-GTTCTACAGTCCGA 44 + |.|| ||..||||| ||.||| ||| || +FC12044_91407 1 ---GTTA--GCTCCCACC------TTAAGATGTT-TA-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 29/43 (67.4%) +# Score: 51.0 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGAGTTCTACAG-TCCGAC------ATG---- 32 + ||| || |||.|| ||| +FC12044_91407 1 --------------GTT----AGCTCCCACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 30.5 +# +# +#======================================= + +Illumina_Smal 1 --------CAAGCAGAAGACGGCATACGA------ 21 + | | || |.||.|| +FC12044_91407 1 GTTAGCTCC---C-----AC--CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 11/39 (28.2%) +# Similarity: 11/39 (28.2%) +# Gaps: 27/39 (69.2%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAG-TCCGACGATC------------ 26 + ||| || |||.|| | +FC12044_91407 1 -------GTT----AGCTCCCAC---CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 9/28 (32.1%) +# Score: 48.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATG---CCGTCTTCTGCTTGT--- 22 + ||..| ||..|||..| .||| +FC12044_91407 1 --GTTAGCTCCCACCTTAAG-ATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 30.5 +# +# +#======================================= + +Illumina_Smal 1 --------CAAGCAGAAGACGGCATACGA------ 21 + | | || |.||.|| +FC12044_91407 1 GTTAGCTCC---C-----AC--CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGA-GTTCTACAGTCCGA 44 + |.|| ||..||||| ||.||| ||| || +FC12044_91407 1 ---GTTA--GCTCCCACC------TTAAGATGTT-TA-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 33/45 (73.3%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACAG-TCCGACGATC------------ 32 + ||| || |||.|| | +FC12044_91407 1 -------------GTT----AGCTCCCAC---CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 20/39 (51.3%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTATG-----CCGT-CTTCTGCTTG 33 + ||| |.|| ||.| |.||. ||| +FC12044_91407 1 -----------CTC-TGTGGCACCCCATCCCTCA-CTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTT-----CCCTACACGACGCTCTTCCGATCT 33 + ||||.| |||..| | |.|.||| +FC12044_91407 1 ---CTCTGTGGCACCCCATC-C--CTCACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 17/61 (27.9%) +# Similarity: 17/61 (27.9%) +# Gaps: 39/61 (63.9%) +# Score: 92.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCT---ACACTCTTTCCCTACACGACGC 47 + | ||| .|||.|..||||| | +FC12044_91407 1 ----------------C-----TCTGTGGCACCCCATCCCT--------C 21 + +Illumina_Geno 48 TCTTCCGATCT 58 + .||| +FC12044_91407 22 ACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 19/39 (48.7%) +# Score: 61.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT----- 34 + |. |.|. ||| || |.|.||| || +FC12044_91407 1 CT--CTGT----GGC--AC---CCCATCC---CTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTT-----CCCTACACGACGCTCTTCCGATCT 33 + ||||.| |||..| | |.|.||| +FC12044_91407 1 ---CTCTGTGGCACCCCATC-C--CTCACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 9/45 (20.0%) +# Similarity: 9/45 (20.0%) +# Gaps: 33/45 (73.3%) +# Score: 21.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATG----CCGAG--------- 32 + | ||.| || ||.|. +FC12044_91407 1 -----------C---TCTG------TGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTT-----CCCTACACGACGCTCTTCCGATCT 33 + ||||.| |||..| | |.|.||| +FC12044_91407 1 ---CTCTGTGGCACCCCATC-C--CTCACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 17/61 (27.9%) +# Similarity: 17/61 (27.9%) +# Gaps: 39/61 (63.9%) +# Score: 92.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCT---ACACTCTTTCCCTACACGACGC 47 + | ||| .|||.|..||||| | +FC12044_91407 1 ----------------C-----TCTGTGGCACCCCATCCCT--------C 21 + +Illumina_Pair 48 TCTTCCGATCT 58 + .||| +FC12044_91407 22 ACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 16/64 (25.0%) +# Similarity: 16/64 (25.0%) +# Gaps: 42/64 (65.6%) +# Score: 86.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTC---GGCATTCCTGCTGAAC 47 + ||| ||||..||..| | +FC12044_91407 1 ----------------------------CTCTGTGGCACCCCATC----C 18 + +Illumina_Pair 48 CGCTCTTCCGATCT 61 + |.|.||| +FC12044_91407 19 CTCACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTT-----CCCTACACGACGCTCTTCCGATCT 33 + ||||.| |||..| | |.|.||| +FC12044_91407 1 ---CTCTGTGGCACCCCATC-C--CTCACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 16/40 (40.0%) +# Similarity: 16/40 (40.0%) +# Gaps: 18/40 (45.0%) +# Score: 86.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTC---GGCATTCCTGCTGAACCGCTCTTCCGATCT 37 + ||| ||||..||..| ||.|.||| +FC12044_91407 1 ----CTCTGTGGCACCCCATC----CCTCACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 12/36 (33.3%) +# Similarity: 12/36 (33.3%) +# Gaps: 21/36 (58.3%) +# Score: 51.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAA--------CTCTGAAC-- 26 + | .||||.|.| ||| || +FC12044_91407 1 ------C--TCTGTGGCACCCCATCCCTC---ACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 8/40 (20.0%) +# Similarity: 8/40 (20.0%) +# Gaps: 29/40 (72.5%) +# Score: 43.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACAGT------CCGAC-------- 26 + || |.|| ||..| +FC12044_91407 1 --------------CT-CTGTGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 11/27 (40.7%) +# Similarity: 11/27 (40.7%) +# Gaps: 8/27 (29.6%) +# Score: 24.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAG-ACGGCAT-----ACGA 21 + |. |.|..| ||..||| ||.. +FC12044_91407 1 CT--CTGTGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 14/28 (50.0%) +# Similarity: 14/28 (50.0%) +# Gaps: 10/28 (35.7%) +# Score: 60.0 +# +# +#======================================= + +Illumina_DpnI 1 -TCGTATG-----CCGT-CTTCTGCTTG 21 + || |.|| ||.| |.||. ||| +FC12044_91407 1 CTC-TGTGGCACCCCATCCCTCA-CTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 11/27 (40.7%) +# Similarity: 11/27 (40.7%) +# Gaps: 8/27 (29.6%) +# Score: 24.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAG-ACGGCAT-----ACGA 21 + |. |.|..| ||..||| ||.. +FC12044_91407 1 CT--CTGTGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 52 +# Identity: 15/52 (28.8%) +# Similarity: 15/52 (28.8%) +# Gaps: 35/52 (67.3%) +# Score: 59.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATAC-----GGCGACC---ACCGACAGGTTCAGAGTTCTACAGTCC 42 + | ||| ||| .|| | ||| ||. +FC12044_91407 1 -------CTCTGTGGC-ACCCCATCC--C----TCA-----CTT------ 25 + +Illumina_DpnI 43 GA 44 + +FC12044_91407 26 -- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 11/44 (25.0%) +# Similarity: 11/44 (25.0%) +# Gaps: 31/44 (70.5%) +# Score: 51.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACAGT----CCGACGATC-------- 32 + || |.|| || |.||| +FC12044_91407 1 ----------------CT-CTGTGGCACC--CCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 16/31 (51.6%) +# Score: 51.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAA--------CTCTGAAC-- 21 + | .||||.|.| ||| || +FC12044_91407 1 -C--TCTGTGGCACCCCATCCCTC---ACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 13/35 (37.1%) +# Similarity: 13/35 (37.1%) +# Gaps: 16/35 (45.7%) +# Score: 44.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACA----GTCC--GACATG 29 + | ||.|.| .|| .||| .||.| +FC12044_91407 1 -C----TCTGTG----GCACCCCATCCCTCACTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 11/29 (37.9%) +# Similarity: 11/29 (37.9%) +# Gaps: 10/29 (34.5%) +# Score: 24.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAG-ACGGCAT-----ACGANN 23 + |. |.|..| ||..||| ||.. +FC12044_91407 1 CT--CTGTGGCACCCCATCCCTCACTT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 14/28 (50.0%) +# Similarity: 14/28 (50.0%) +# Gaps: 10/28 (35.7%) +# Score: 60.0 +# +# +#======================================= + +Illumina_NlaI 1 -TCGTATG-----CCGT-CTTCTGCTTG 21 + || |.|| ||.| |.||. ||| +FC12044_91407 1 CTC-TGTGGCACCCCATCCCTCA-CTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 11/27 (40.7%) +# Similarity: 11/27 (40.7%) +# Gaps: 8/27 (29.6%) +# Score: 24.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAG-ACGGCAT-----ACGA 21 + |. |.|..| ||..||| ||.. +FC12044_91407 1 CT--CTGTGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 52 +# Identity: 15/52 (28.8%) +# Similarity: 15/52 (28.8%) +# Gaps: 35/52 (67.3%) +# Score: 59.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATAC-----GGCGACC---ACCGACAGGTTCAGAGTTCTACAGTCC 42 + | ||| ||| .|| | ||| ||. +FC12044_91407 1 -------CTCTGTGGC-ACCCCATCC--C----TCA-----CTT------ 25 + +Illumina_NlaI 43 GA 44 + +FC12044_91407 26 -- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 29/43 (67.4%) +# Score: 46.5 +# +# +#======================================= + +Illumina_NlaI 1 -----------CCGACAGGTTCAGAGTTCTACAGTCCGACATG 32 + || || || | | || ||.| +FC12044_91407 1 CTCTGTGGCACCC--CA---TC------C--C--TC--ACTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 11/27 (40.7%) +# Similarity: 11/27 (40.7%) +# Gaps: 8/27 (29.6%) +# Score: 24.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAG-ACGGCAT-----ACGA 21 + |. |.|..| ||..||| ||.. +FC12044_91407 1 CT--CTGTGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 11/38 (28.9%) +# Similarity: 11/38 (28.9%) +# Gaps: 25/38 (65.8%) +# Score: 51.0 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAGT----CCGACGATC-------- 26 + || |.|| || |.||| +FC12044_91407 1 ----------CT-CTGTGGCACC--CCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 14/29 (48.3%) +# Similarity: 14/29 (48.3%) +# Gaps: 11/29 (37.9%) +# Score: 60.0 +# +# +#======================================= + +Illumina_Smal 1 -TCGTATG-----CCGT-CTTCTGCTTGT 22 + || |.|| ||.| |.||. ||| +FC12044_91407 1 CTC-TGTGGCACCCCATCCCTCA-CTT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 11/27 (40.7%) +# Similarity: 11/27 (40.7%) +# Gaps: 8/27 (29.6%) +# Score: 24.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAG-ACGGCAT-----ACGA 21 + |. |.|..| ||..||| ||.. +FC12044_91407 1 CT--CTGTGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 52 +# Identity: 15/52 (28.8%) +# Similarity: 15/52 (28.8%) +# Gaps: 35/52 (67.3%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATAC-----GGCGACC---ACCGACAGGTTCAGAGTTCTACAGTCC 42 + | ||| ||| .|| | ||| ||. +FC12044_91407 1 -------CTCTGTGGC-ACCCCATCC--C----TCA-----CTT------ 25 + +Illumina_Smal 43 GA 44 + +FC12044_91407 26 -- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 11/44 (25.0%) +# Similarity: 11/44 (25.0%) +# Gaps: 31/44 (70.5%) +# Score: 51.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACAGT----CCGACGATC-------- 32 + || |.|| || |.||| +FC12044_91407 1 ----------------CT-CTGTGGCACC--CCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 12/40 (30.0%) +# Similarity: 12/40 (30.0%) +# Gaps: 22/40 (55.0%) +# Score: 37.5 +# +# +#======================================= + +Illumina_Geno 1 GAT-------CGGAAGAGCTCGTATGCCGTCTTCTGCTTG 33 + ||| |...|.| |||| |.|. +FC12044_91407 1 GATTTTTTAACAATAAA---CGTA------------CATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 33.5 +# +# +#======================================= + +Illumina_Geno 1 -ACACTCTTTCCCTACAC----GACGCTCTTCCGATCT 33 + | |.||| || || .|||..|.| | +FC12044_91407 1 GA---TTTTT---TA-ACAATAAACGTACAT---A--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 69 +# Identity: 13/69 (18.8%) +# Similarity: 13/69 (18.8%) +# Gaps: 55/69 (79.7%) +# Score: 47.0 +# +# +#======================================= + +Illumina_Geno 1 -----------AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTA 39 + ||| |.||| |||| || +FC12044_91407 1 GATTTTTTAACAAT-AAACG---------------TACA---------TA 25 + +Illumina_Geno 40 CACGACGCTCTTCCGATCT 58 + +FC12044_91407 26 ------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 31/45 (68.9%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Geno 1 ----------CAAGCAGAAGACG-GCATACGAGCTCTTCCGATCT 34 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA---------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 33.5 +# +# +#======================================= + +Illumina_Geno 1 -ACACTCTTTCCCTACAC----GACGCTCTTCCGATCT 33 + | |.||| || || .|||..|.| | +FC12044_91407 1 GA---TTTTT---TA-ACAATAAACGTACAT---A--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 12/39 (30.8%) +# Similarity: 12/39 (30.8%) +# Gaps: 21/39 (53.8%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Pair 1 GAT-------CGGAAGAGCGGTTCAGCAGGAATGCCGAG 32 + ||| | .|..|.| ||.|| .| +FC12044_91407 1 GATTTTTTAAC-AATAAAC-GTACA-----TA------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 33.5 +# +# +#======================================= + +Illumina_Pair 1 -ACACTCTTTCCCTACAC----GACGCTCTTCCGATCT 33 + | |.||| || || .|||..|.| | +FC12044_91407 1 GA---TTTTT---TA-ACAATAAACGTACAT---A--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 69 +# Identity: 13/69 (18.8%) +# Similarity: 13/69 (18.8%) +# Gaps: 55/69 (79.7%) +# Score: 47.0 +# +# +#======================================= + +Illumina_Pair 1 -----------AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTA 39 + ||| |.||| |||| || +FC12044_91407 1 GATTTTTTAACAAT-AAACG---------------TACA---------TA 25 + +Illumina_Pair 40 CACGACGCTCTTCCGATCT 58 + +FC12044_91407 26 ------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 72 +# Identity: 11/72 (15.3%) +# Similarity: 11/72 (15.3%) +# Gaps: 58/72 (80.6%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Pair 1 ----------CAAGCAGAAGACG-GCATACGAGATCGGTCTCGGCATTCC 39 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA--------------------- 25 + +Illumina_Pair 40 TGCTGAACCGCTCTTCCGATCT 61 + +FC12044_91407 26 ---------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 33.5 +# +# +#======================================= + +Illumina_Pair 1 -ACACTCTTTCCCTACAC----GACGCTCTTCCGATCT 33 + | |.||| || || .|||..|.| | +FC12044_91407 1 GA---TTTTT---TA-ACAATAAACGTACAT---A--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 24/43 (55.8%) +# Score: 28.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAAC------CGCTCTTCCGATCT 37 + |.| .|| |.||| ||..|.| | +FC12044_91407 1 -GAT------TTT-----TTAACAATAAACGTACAT---A--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 12/33 (36.4%) +# Similarity: 12/33 (36.4%) +# Gaps: 15/33 (45.5%) +# Score: 48.5 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAACTCTGAAC------- 26 + ||| |.|..|||..|.||| +FC12044_91407 1 GAT--------TTTTTAACAATAAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 14/33 (42.4%) +# Similarity: 14/33 (42.4%) +# Gaps: 15/33 (45.5%) +# Score: 41.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCT-ACAGT--CCG-AC--- 26 + |.|| ||.| |||.| .|| || +FC12044_91407 1 ---GATT-----TTTTAACAATAAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 52.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------CAAGCAGAAGACG-GCATACGA 21 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 6/37 (16.2%) +# Similarity: 6/37 (16.2%) +# Gaps: 28/37 (75.7%) +# Score: 26.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------------TCGTATGCCGTCTTCTGCTTG 21 + .|||| |.|. +FC12044_91407 1 GATTTTTTAACAATAAACGTA------------CATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 52.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------CAAGCAGAAGACG-GCATACGA 21 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 55 +# Identity: 12/55 (21.8%) +# Similarity: 12/55 (21.8%) +# Gaps: 41/55 (74.5%) +# Score: 41.5 +# +# +#======================================= + +Illumina_DpnI 1 -----------AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG 39 + ||| |.|| ||.|| || +FC12044_91407 1 GATTTTTTAACAAT-AAAC---------------GTACA------TA--- 25 + +Illumina_DpnI 40 TCCGA 44 + +FC12044_91407 26 ----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 14/36 (38.9%) +# Similarity: 14/36 (38.9%) +# Gaps: 15/36 (41.7%) +# Score: 48.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCT-ACAGTCCGACGATC--- 32 + |.|| ||.| |||.| ..|||..| +FC12044_91407 1 -----GATT-----TTTTAACAAT-AAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 11/28 (39.3%) +# Similarity: 11/28 (39.3%) +# Gaps: 10/28 (35.7%) +# Score: 47.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAACTCTGAAC------- 21 + ||.|.|..|||..|.||| +FC12044_91407 1 ---GATTTTTTAACAATAAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 16/33 (48.5%) +# Similarity: 16/33 (48.5%) +# Gaps: 12/33 (36.4%) +# Score: 52.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCT-ACAGT--CCG-ACATG 29 + |.|| ||.| |||.| .|| ||||. +FC12044_91407 1 ---GATT-----TTTTAACAATAAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 20/34 (58.8%) +# Score: 52.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------CAAGCAGAAGACG-GCATACGANN 23 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 6/37 (16.2%) +# Similarity: 6/37 (16.2%) +# Gaps: 28/37 (75.7%) +# Score: 26.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------------TCGTATGCCGTCTTCTGCTTG 21 + .|||| |.|. +FC12044_91407 1 GATTTTTTAACAATAAACGTA------------CATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 52.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------CAAGCAGAAGACG-GCATACGA 21 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 55 +# Identity: 12/55 (21.8%) +# Similarity: 12/55 (21.8%) +# Gaps: 41/55 (74.5%) +# Score: 41.5 +# +# +#======================================= + +Illumina_NlaI 1 -----------AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG 39 + ||| |.|| ||.|| || +FC12044_91407 1 GATTTTTTAACAAT-AAAC---------------GTACA------TA--- 25 + +Illumina_NlaI 40 TCCGA 44 + +FC12044_91407 26 ----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 15/36 (41.7%) +# Score: 52.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGAGTTCT-ACAGT--CCG-ACATG 32 + |.|| ||.| |||.| .|| ||||. +FC12044_91407 1 ------GATT-----TTTTAACAATAAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Smal 1 ----------CAAGCAGAAGACG-GCATACGA 21 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 13/31 (41.9%) +# Similarity: 13/31 (41.9%) +# Gaps: 11/31 (35.5%) +# Score: 47.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCT--ACAGTCCGACGATC--- 26 + ||.||.| |||.| ..|||..| +FC12044_91407 1 -----GATTTTTTAACAAT-AAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 6/38 (15.8%) +# Similarity: 6/38 (15.8%) +# Gaps: 29/38 (76.3%) +# Score: 26.5 +# +# +#======================================= + +Illumina_Smal 1 ----------------TCGTATGCCGTCTTCTGCTTGT 22 + .|||| |.|. +FC12044_91407 1 GATTTTTTAACAATAAACGTA------------CATA- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Smal 1 ----------CAAGCAGAAGACG-GCATACGA 21 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 55 +# Identity: 12/55 (21.8%) +# Similarity: 12/55 (21.8%) +# Gaps: 41/55 (74.5%) +# Score: 41.5 +# +# +#======================================= + +Illumina_Smal 1 -----------AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG 39 + ||| |.|| ||.|| || +FC12044_91407 1 GATTTTTTAACAAT-AAAC---------------GTACA------TA--- 25 + +Illumina_Smal 40 TCCGA 44 + +FC12044_91407 26 ----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 14/36 (38.9%) +# Similarity: 14/36 (38.9%) +# Gaps: 15/36 (41.7%) +# Score: 48.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCT-ACAGTCCGACGATC--- 32 + |.|| ||.| |||.| ..|||..| +FC12044_91407 1 -----GATT-----TTTTAACAAT-AAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 20/39 (51.3%) +# Score: 67.5 +# +# +#======================================= + +Illumina_Geno 1 GAT---CGGAAGAGCTCGTATGCCGTCTT---CTGCTTG 33 + |.| |.| ||| |||||| ||.| +FC12044_91407 1 GTTGCCCAG----GCT-------CGTCTTGAACTCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCT-ACACGACGCTCTTCCGATCT-- 33 + .||.|||. .|.|| |||| ||.|| +FC12044_91407 1 -----GTTGCCCAGGCTCG----TCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 16/61 (26.2%) +# Similarity: 16/61 (26.2%) +# Gaps: 39/61 (63.9%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCT-ACACGACGCTC 49 + . ||.|||. .|.|| || +FC12044_91407 1 -----------------------------G-TTGCCCAGGCTCG----TC 16 + +Illumina_Geno 50 TTCCGATCT-- 58 + || ||.|| +FC12044_91407 17 TT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 21/40 (52.5%) +# Score: 69.0 +# +# +#======================================= + +Illumina_Geno 1 ------CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT 34 + ||.|| .||.|.| ||.||| | +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTT--GAACTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCT-ACACGACGCTCTTCCGATCT-- 33 + .||.|||. .|.|| |||| ||.|| +FC12044_91407 1 -----GTTGCCCAGGCTCG----TCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 13/42 (31.0%) +# Similarity: 13/42 (31.0%) +# Gaps: 27/42 (64.3%) +# Score: 46.5 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTT---CAG------CAGGAA-TGCCGAG 32 + ||| ||| |..||| | || +FC12044_91407 1 -------------GTTGCCCAGGCTCGTCTTGAACT-CC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCT-ACACGACGCTCTTCCGATCT-- 33 + .||.|||. .|.|| |||| ||.|| +FC12044_91407 1 -----GTTGCCCAGGCTCG----TCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 16/61 (26.2%) +# Similarity: 16/61 (26.2%) +# Gaps: 39/61 (63.9%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCT-ACACGACGCTC 49 + . ||.|||. .|.|| || +FC12044_91407 1 -----------------------------G-TTGCCCAGGCTCG----TC 16 + +Illumina_Pair 50 TTCCGATCT-- 58 + || ||.|| +FC12044_91407 17 TT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 19/64 (29.7%) +# Similarity: 19/64 (29.7%) +# Gaps: 42/64 (65.6%) +# Score: 83.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGAT---CGGTCTCGGCATTCCTGCTGAAC 47 + |.| |.|.|||| | || ||||| +FC12044_91407 1 ---------------------GTTGCCCAGGCTCG---T--CT--TGAAC 22 + +Illumina_Pair 48 CGCTCTTCCGATCT 61 + ||| +FC12044_91407 23 ------TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCT-ACACGACGCTCTTCCGATCT-- 33 + .||.|||. .|.|| |||| ||.|| +FC12044_91407 1 -----GTTGCCCAGGCTCG----TCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 17/43 (39.5%) +# Similarity: 17/43 (39.5%) +# Gaps: 24/43 (55.8%) +# Score: 82.5 +# +# +#======================================= + +Illumina_Pair 1 ------CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT 37 + |.|.|||| | || ||||| ||| +FC12044_91407 1 GTTGCCCAGGCTCG---T--CT--TGAAC------TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 14/35 (40.0%) +# Similarity: 14/35 (40.0%) +# Gaps: 19/35 (54.3%) +# Score: 77.0 +# +# +#======================================= + +Illumina_DpnI 1 ---------GATCGTCGGACTGTAGAACTCTGAAC 26 + |.||||| |.|||||| | +FC12044_91407 1 GTTGCCCAGGCTCGTC------TTGAACTC----C 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 14/34 (41.2%) +# Similarity: 14/34 (41.2%) +# Gaps: 17/34 (50.0%) +# Score: 63.0 +# +# +#======================================= + +Illumina_DpnI 1 -----ACAGGTTCAGAGTTCT---ACAGTCCGAC 26 + .||||.||. ||| || ||| +FC12044_91407 1 GTTGCCCAGGCTCG----TCTTGAAC--TCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 44.5 +# +# +#======================================= + +Illumina_DpnI 1 ------CAAGCAGAAGACGGCATACGA----- 21 + ||.|| .||.|.| || +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 14/31 (45.2%) +# Similarity: 14/31 (45.2%) +# Gaps: 16/31 (51.6%) +# Score: 67.0 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGC-------CGTCTT---CTGCTTG 21 + || ||| |||||| ||.| +FC12044_91407 1 --GT-TGCCCAGGCTCGTCTTGAACTCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 44.5 +# +# +#======================================= + +Illumina_DpnI 1 ------CAAGCAGAAGACGGCATACGA----- 21 + ||.|| .||.|.| || +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 75.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCT---ACAGTCCGA 44 + |.|.| ||| |.| ||. ||| || ||| +FC12044_91407 1 ---GTTGC-----CCA--GGC----TCG----TCTTGAAC--TCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 15/40 (37.5%) +# Similarity: 15/40 (37.5%) +# Gaps: 23/40 (57.5%) +# Score: 68.5 +# +# +#======================================= + +Illumina_DpnI 1 -----CGACAGGTTCAGAGTTCT---ACAGTCCGACGATC 32 + | ||||.||. ||| || ||| +FC12044_91407 1 GTTGCC--CAGGCTCG----TCTTGAAC--TCC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 61.0 +# +# +#======================================= + +Illumina_NlaI 1 -----------TCGGACTGTAGAACTCTGAAC 21 + || |.| |.|||||| | +FC12044_91407 1 GTTGCCCAGGCTC-GTC--TTGAACTC----C 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 14/37 (37.8%) +# Similarity: 14/37 (37.8%) +# Gaps: 20/37 (54.1%) +# Score: 63.0 +# +# +#======================================= + +Illumina_NlaI 1 -----ACAGGTTCAGAGTTCT---ACAGTCCGACATG 29 + .||||.||. ||| || ||| +FC12044_91407 1 GTTGCCCAGGCTCG----TCTTGAAC--TCC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 20/34 (58.8%) +# Score: 39.0 +# +# +#======================================= + +Illumina_NlaI 1 ------CAAGCAGAAGACGGCATA-----CGANN 23 + ||.|| .||.|.|. | +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTTGAACTCC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 14/31 (45.2%) +# Similarity: 14/31 (45.2%) +# Gaps: 16/31 (51.6%) +# Score: 67.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGC-------CGTCTT---CTGCTTG 21 + || ||| |||||| ||.| +FC12044_91407 1 --GT-TGCCCAGGCTCGTCTTGAACTCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 44.5 +# +# +#======================================= + +Illumina_NlaI 1 ------CAAGCAGAAGACGGCATACGA----- 21 + ||.|| .||.|.| || +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 75.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCT---ACAGTCCGA 44 + |.|.| ||| |.| ||. ||| || ||| +FC12044_91407 1 ---GTTGC-----CCA--GGC----TCG----TCTTGAAC--TCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 21/39 (53.8%) +# Score: 78.5 +# +# +#======================================= + +Illumina_NlaI 1 ----CCGACAGGTTCAGAGTTCT---ACAGTCCGACATG 32 + || ||||.||. ||| || ||| +FC12044_91407 1 GTTGCC--CAGGCTCG----TCTTGAAC--TCC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 44.5 +# +# +#======================================= + +Illumina_Smal 1 ------CAAGCAGAAGACGGCATACGA----- 21 + ||.|| .||.|.| || +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 15/34 (44.1%) +# Similarity: 15/34 (44.1%) +# Gaps: 17/34 (50.0%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Smal 1 GTT---CAGAGT--TCT---ACAGTCCGACGATC 26 + ||| |||..| ||| || ||| +FC12044_91407 1 GTTGCCCAGGCTCGTCTTGAAC--TCC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 14/32 (43.8%) +# Similarity: 14/32 (43.8%) +# Gaps: 17/32 (53.1%) +# Score: 67.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGC-------CGTCTT---CTGCTTGT 22 + || ||| |||||| ||.| +FC12044_91407 1 --GT-TGCCCAGGCTCGTCTTGAACTCC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 44.5 +# +# +#======================================= + +Illumina_Smal 1 ------CAAGCAGAAGACGGCATACGA----- 21 + ||.|| .||.|.| || +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 75.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCT---ACAGTCCGA 44 + |.|.| ||| |.| ||. ||| || ||| +FC12044_91407 1 ---GTTGC-----CCA--GGC----TCG----TCTTGAAC--TCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 15/40 (37.5%) +# Similarity: 15/40 (37.5%) +# Gaps: 23/40 (57.5%) +# Score: 68.5 +# +# +#======================================= + +Illumina_Smal 1 -----CGACAGGTTCAGAGTTCT---ACAGTCCGACGATC 32 + | ||||.||. ||| || ||| +FC12044_91407 1 GTTGCC--CAGGCTCG----TCTTGAAC--TCC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 18/37 (48.6%) +# Similarity: 18/37 (48.6%) +# Gaps: 16/37 (43.2%) +# Score: 55.0 +# +# +#======================================= + +Illumina_Geno 1 -GATCGGAAGAGCTCGTATGCCGT--C-TTCTGCTTG 33 + ||| .|||| |||.| || | |.||| +FC12044_91407 1 TGAT-TGAAG-----GTAGG--GTAGCATACTG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 6/50 (12.0%) +# Similarity: 6/50 (12.0%) +# Gaps: 42/50 (84.0%) +# Score: 18.0 +# +# +#======================================= + +Illumina_Geno 1 -----------------ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| |||. | +FC12044_91407 1 TGATTGAAGGTAGGGTAGCA---------TACT-G--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 17/62 (27.4%) +# Similarity: 17/62 (27.4%) +# Gaps: 41/62 (66.1%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATC---TA-CACTCTTTCCCTACACGACG 46 + ||||. || ||.|. || || |||. | +FC12044_91407 1 --TGATT----GA------AGGTAGGGTAGCA---------TACT-G--- 25 + +Illumina_Geno 47 CTCTTCCGATCT 58 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 27/43 (62.8%) +# Score: 41.0 +# +# +#======================================= + +Illumina_Geno 1 -----CAAGCAGAAGACG---GCATAC-GAGCTCTTCCGATCT 34 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG-------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 6/50 (12.0%) +# Similarity: 6/50 (12.0%) +# Gaps: 42/50 (84.0%) +# Score: 18.0 +# +# +#======================================= + +Illumina_Geno 1 -----------------ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| |||. | +FC12044_91407 1 TGATTGAAGGTAGGGTAGCA---------TACT-G--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 19/35 (54.3%) +# Similarity: 19/35 (54.3%) +# Gaps: 13/35 (37.1%) +# Score: 56.5 +# +# +#======================================= + +Illumina_Pair 1 -GATCGGAAG--AGCGGTTCAGCAGGAATGCCGAG 32 + ||| .|||| || ||| ||| ||.|.| +FC12044_91407 1 TGAT-TGAAGGTAG-GGT--AGC----ATACTG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 6/50 (12.0%) +# Similarity: 6/50 (12.0%) +# Gaps: 42/50 (84.0%) +# Score: 18.0 +# +# +#======================================= + +Illumina_Pair 1 -----------------ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| |||. | +FC12044_91407 1 TGATTGAAGGTAGGGTAGCA---------TACT-G--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 17/62 (27.4%) +# Similarity: 17/62 (27.4%) +# Gaps: 41/62 (66.1%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATC---TA-CACTCTTTCCCTACACGACG 46 + ||||. || ||.|. || || |||. | +FC12044_91407 1 --TGATT----GA------AGGTAGGGTAGCA---------TACT-G--- 25 + +Illumina_Pair 47 CTCTTCCGATCT 58 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 19/62 (30.6%) +# Similarity: 19/62 (30.6%) +# Gaps: 38/62 (61.3%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGA-AGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCG 49 + .|| .||.|| || | ||| .||||. ||| +FC12044_91407 1 -----TGATTGAAGG--TA-G-----GGT---AGCATA-CTG-------- 25 + +Illumina_Pair 50 CTCTTCCGATCT 61 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 6/50 (12.0%) +# Similarity: 6/50 (12.0%) +# Gaps: 42/50 (84.0%) +# Score: 18.0 +# +# +#======================================= + +Illumina_Pair 1 -----------------ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| |||. | +FC12044_91407 1 TGATTGAAGGTAGGGTAGCA---------TACT-G--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 49 +# Identity: 10/49 (20.4%) +# Similarity: 10/49 (20.4%) +# Gaps: 36/49 (73.5%) +# Score: 38.0 +# +# +#======================================= + +Illumina_Pair 1 ------------CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT 37 + .||| .||||. ||| +FC12044_91407 1 TGATTGAAGGTAGGGT---AGCATA-CTG-------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 14/31 (45.2%) +# Similarity: 14/31 (45.2%) +# Gaps: 11/31 (35.5%) +# Score: 49.0 +# +# +#======================================= + +Illumina_DpnI 1 -----GATCGTCGGACTGTAGAACTCTGAAC 26 + ||..||.|| ||||.|..||| +FC12044_91407 1 TGATTGAAGGTAGG---GTAGCATACTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 14/34 (41.2%) +# Similarity: 14/34 (41.2%) +# Gaps: 17/34 (50.0%) +# Score: 50.5 +# +# +#======================================= + +Illumina_DpnI 1 ------ACAGGTTCAGAGTT-C-TACAGTCCGAC 26 + | |||| ||.||. | |||.| +FC12044_91407 1 TGATTGA-AGGT--AGGGTAGCATACTG------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_DpnI 1 -----CAAGCAGAAGACG---GCATAC-GA 21 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 30.0 +# +# +#======================================= + +Illumina_DpnI 1 -------TCGTATGCCGT--C-TTCTGCTTG 21 + ..|||.| || | |.||| +FC12044_91407 1 TGATTGAAGGTAGG--GTAGCATACTG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_DpnI 1 -----CAAGCAGAAGACG---GCATAC-GA 21 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 19/46 (41.3%) +# Similarity: 19/46 (41.3%) +# Gaps: 23/46 (50.0%) +# Score: 66.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTT-C-TACAGTCCGA 44 + ||||. || |||| ||.||. | |||.| +FC12044_91407 1 --TGATT----GA--------AGGT--AGGGTAGCATACTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 19/38 (50.0%) +# Score: 48.5 +# +# +#======================================= + +Illumina_DpnI 1 ----CGACAGGTTCAGAGTT-C-TACAGTCCGACGATC 32 + .|| |||| ||.||. | |||.| +FC12044_91407 1 TGATTGA-AGGT--AGGGTAGCATACTG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 41.0 +# +# +#======================================= + +Illumina_NlaI 1 ----------TCGGACTGTAGAACTCTGAAC 21 + |.|| ||||.|..||| +FC12044_91407 1 TGATTGAAGGTAGG---GTAGCATACTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 14/37 (37.8%) +# Similarity: 14/37 (37.8%) +# Gaps: 20/37 (54.1%) +# Score: 50.5 +# +# +#======================================= + +Illumina_NlaI 1 ------ACAGGTTCAGAGTT-C-TACAGTCCGACATG 29 + | |||| ||.||. | |||.| +FC12044_91407 1 TGATTGA-AGGT--AGGGTAGCATACTG--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 14/32 (43.8%) +# Similarity: 14/32 (43.8%) +# Gaps: 16/32 (50.0%) +# Score: 41.0 +# +# +#======================================= + +Illumina_NlaI 1 -----CAAGCAGAAGACG---GCATAC-GANN 23 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 30.0 +# +# +#======================================= + +Illumina_NlaI 1 -------TCGTATGCCGT--C-TTCTGCTTG 21 + ..|||.| || | |.||| +FC12044_91407 1 TGATTGAAGGTAGG--GTAGCATACTG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_NlaI 1 -----CAAGCAGAAGACG---GCATAC-GA 21 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 19/46 (41.3%) +# Similarity: 19/46 (41.3%) +# Gaps: 23/46 (50.0%) +# Score: 66.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTT-C-TACAGTCCGA 44 + ||||. || |||| ||.||. | |||.| +FC12044_91407 1 --TGATT----GA--------AGGT--AGGGTAGCATACTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 21/39 (53.8%) +# Score: 46.0 +# +# +#======================================= + +Illumina_NlaI 1 -----CCGACAGGTTCAGAGTT-C-TACAGTCCGACATG 32 + || |||| ||.||. | |||.| +FC12044_91407 1 TGATT--GA-AGGT--AGGGTAGCATACTG--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_Smal 1 -----CAAGCAGAAGACG---GCATAC-GA 21 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 11/37 (29.7%) +# Similarity: 11/37 (29.7%) +# Gaps: 23/37 (62.2%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Smal 1 ---------GTTCAGAGTT-C-TACAGTCCGACGATC 26 + || ||.||. | |||.| +FC12044_91407 1 TGATTGAAGGT--AGGGTAGCATACTG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 17/32 (53.1%) +# Score: 30.0 +# +# +#======================================= + +Illumina_Smal 1 -------TCGTATGCCGT--C-TTCTGCTTGT 22 + ..|||.| || | |.||| +FC12044_91407 1 TGATTGAAGGTAGG--GTAGCATACTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_Smal 1 -----CAAGCAGAAGACG---GCATAC-GA 21 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 19/46 (41.3%) +# Similarity: 19/46 (41.3%) +# Gaps: 23/46 (50.0%) +# Score: 66.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTT-C-TACAGTCCGA 44 + ||||. || |||| ||.||. | |||.| +FC12044_91407 1 --TGATT----GA--------AGGT--AGGGTAGCATACTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 19/38 (50.0%) +# Score: 48.5 +# +# +#======================================= + +Illumina_Smal 1 ----CGACAGGTTCAGAGTT-C-TACAGTCCGACGATC 32 + .|| |||| ||.||. | |||.| +FC12044_91407 1 TGATTGA-AGGT--AGGGTAGCATACTG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 50.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTAT---GCCG-----TCTTCTGCTTG 33 + ||||..|| ||.| .| ||| +FC12044_91407 1 ----------GCTCCAATAGCGCAGAGGAAAC--CTG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 9/48 (18.8%) +# Similarity: 9/48 (18.8%) +# Gaps: 38/48 (79.2%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGACGCTCTTCCGAT--------------CT- 33 + || |||.|| || +FC12044_91407 1 --------------------GC---TCCAATAGCGCAGAGGAAACCTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 14/64 (21.9%) +# Similarity: 14/64 (21.9%) +# Gaps: 45/64 (70.3%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCA------CCGAGATCTACACTCTTTCCCTACACGA 44 + || .||| |.|||. |.| |||. +FC12044_91407 1 ---------GC-TCCAATAGCGCAGAGG---AAA-------CCTG----- 25 + +Illumina_Geno 45 CGCTCTTCCGATCT 58 + +FC12044_91407 26 -------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 27/43 (62.8%) +# Score: 58.5 +# +# +#======================================= + +Illumina_Geno 1 ----CAA----GCAGAAGACGGCATACGAGCTCTTCCGATCT- 34 + ||| |||||.| |.|| || +FC12044_91407 1 GCTCCAATAGCGCAGAGG-----AAAC-------------CTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 9/48 (18.8%) +# Similarity: 9/48 (18.8%) +# Gaps: 38/48 (79.2%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGACGCTCTTCCGAT--------------CT- 33 + || |||.|| || +FC12044_91407 1 --------------------GC---TCCAATAGCGCAGAGGAAACCTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 20/33 (60.6%) +# Similarity: 20/33 (60.6%) +# Gaps: 9/33 (27.3%) +# Score: 68.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATGCC-GAG 32 + |.|| .||.|||| ||| |||||. || | +FC12044_91407 1 GCTC-CAATAGCG---CAG-AGGAAA-CCTG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 9/48 (18.8%) +# Similarity: 9/48 (18.8%) +# Gaps: 38/48 (79.2%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGACGCTCTTCCGAT--------------CT- 33 + || |||.|| || +FC12044_91407 1 --------------------GC---TCCAATAGCGCAGAGGAAACCTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 14/64 (21.9%) +# Similarity: 14/64 (21.9%) +# Gaps: 45/64 (70.3%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCA------CCGAGATCTACACTCTTTCCCTACACGA 44 + || .||| |.|||. |.| |||. +FC12044_91407 1 ---------GC-TCCAATAGCGCAGAGG---AAA-------CCTG----- 25 + +Illumina_Pair 45 CGCTCTTCCGATCT 58 + +FC12044_91407 26 -------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 69 +# Identity: 14/69 (20.3%) +# Similarity: 14/69 (20.3%) +# Gaps: 52/69 (75.4%) +# Score: 64.0 +# +# +#======================================= + +Illumina_Pair 1 ----CAA----GCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGC 42 + ||| |||||.| |..|||| +FC12044_91407 1 GCTCCAATAGCGCAGAGG------------------------AAACCTG- 25 + +Illumina_Pair 43 TGAACCGCTCTTCCGATCT 61 + +FC12044_91407 26 ------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 9/48 (18.8%) +# Similarity: 9/48 (18.8%) +# Gaps: 38/48 (79.2%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGACGCTCTTCCGAT--------------CT- 33 + || |||.|| || +FC12044_91407 1 --------------------GC---TCCAATAGCGCAGAGGAAACCTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 16/44 (36.4%) +# Similarity: 16/44 (36.4%) +# Gaps: 26/44 (59.1%) +# Score: 61.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATT--CCTGCTG----AACC-GCTCTTCCGATCT 37 + | ||| ||.| | ||.| |||| | +FC12044_91407 1 -G--CTC--CAATAGC--GCAGAGGAAACCTG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 13/35 (37.1%) +# Similarity: 13/35 (37.1%) +# Gaps: 19/35 (54.3%) +# Score: 51.5 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAG---------AACTCTGAAC 26 + |.|| |..||| ||| ||| +FC12044_91407 1 GCTC------CAATAGCGCAGAGGAAAC-CTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 12/35 (34.3%) +# Similarity: 12/35 (34.3%) +# Gaps: 19/35 (54.3%) +# Score: 31.5 +# +# +#======================================= + +Illumina_DpnI 1 ------ACAG-GTTCAGAG--TTCTACAGTCCGAC 26 + |.|| | ||||| ..| |.| +FC12044_91407 1 GCTCCAATAGCG--CAGAGGAAAC--CTG------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 53.5 +# +# +#======================================= + +Illumina_DpnI 1 ----CAA----GCAGAAGA---CGGCATACGA 21 + ||| |||||.|| |.| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAACCTG------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 34.5 +# +# +#======================================= + +Illumina_DpnI 1 --TCGTAT---GCCG-----TCTTCTGCTTG 21 + ||..|| ||.| .| ||| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAAC--CTG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 53.5 +# +# +#======================================= + +Illumina_DpnI 1 ----CAA----GCAGAAGA---CGGCATACGA 21 + ||| |||||.|| |.| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAACCTG------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 15/47 (31.9%) +# Similarity: 15/47 (31.9%) +# Gaps: 25/47 (53.2%) +# Score: 54.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAG-GTTCAGAG--TTCTACAGTCCGA 44 + . |.||.|.|| | ||||| ..| |.| +FC12044_91407 1 ------------G-CTCCAATAGCG--CAGAGGAAAC--CTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 13/39 (33.3%) +# Similarity: 13/39 (33.3%) +# Gaps: 21/39 (53.8%) +# Score: 39.5 +# +# +#======================================= + +Illumina_DpnI 1 ----CGACAG-GTTCAGAG--TTCTACAGTCCGACGATC 32 + |.|.|| | ||||| ..| |.| +FC12044_91407 1 GCTCCAATAGCG--CAGAGGAAAC--CTG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 53.0 +# +# +#======================================= + +Illumina_NlaI 1 --TCGGACTGTAG---------AACTCTGAAC 21 + || |..||| ||| ||| +FC12044_91407 1 GCTC---CAATAGCGCAGAGGAAAC-CTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 12/38 (31.6%) +# Similarity: 12/38 (31.6%) +# Gaps: 22/38 (57.9%) +# Score: 37.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAGTC-CG--------ACATG 29 + | ||.||.|. || ||.|| +FC12044_91407 1 -----------G--CTCCAATAGCGCAGAGGAAACCTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 12/34 (35.3%) +# Similarity: 12/34 (35.3%) +# Gaps: 20/34 (58.8%) +# Score: 53.5 +# +# +#======================================= + +Illumina_NlaI 1 ----CAA----GCAGAAGA---CGGCATACGANN 23 + ||| |||||.|| |.| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAACCTG--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 34.5 +# +# +#======================================= + +Illumina_NlaI 1 --TCGTAT---GCCG-----TCTTCTGCTTG 21 + ||..|| ||.| .| ||| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAAC--CTG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 53.5 +# +# +#======================================= + +Illumina_NlaI 1 ----CAA----GCAGAAGA---CGGCATACGA 21 + ||| |||||.|| |.| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAACCTG------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 15/47 (31.9%) +# Similarity: 15/47 (31.9%) +# Gaps: 25/47 (53.2%) +# Score: 54.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAG-GTTCAGAG--TTCTACAGTCCGA 44 + . |.||.|.|| | ||||| ..| |.| +FC12044_91407 1 ------------G-CTCCAATAGCG--CAGAGGAAAC--CTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 15/36 (41.7%) +# Score: 54.0 +# +# +#======================================= + +Illumina_NlaI 1 ---CCGACAG-GTTCAGAGTTCTACAGTCCGACATG 32 + ||.|.|| | |||||. |..|| || +FC12044_91407 1 GCTCCAATAGCG--CAGAGG-----AAACC----TG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 53.5 +# +# +#======================================= + +Illumina_Smal 1 ----CAA----GCAGAAGA---CGGCATACGA 21 + ||| |||||.|| |.| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAACCTG------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 9/39 (23.1%) +# Similarity: 9/39 (23.1%) +# Gaps: 27/39 (69.2%) +# Score: 35.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAGTCCGA---CG-------ATC--- 26 + .| |||.| || |.| +FC12044_91407 1 ------------GC--TCCAATAGCGCAGAGGAAACCTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 17/32 (53.1%) +# Score: 34.5 +# +# +#======================================= + +Illumina_Smal 1 --TCGTAT---GCCG-----TCTTCTGCTTGT 22 + ||..|| ||.| .| ||| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAAC--CTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 53.5 +# +# +#======================================= + +Illumina_Smal 1 ----CAA----GCAGAAGA---CGGCATACGA 21 + ||| |||||.|| |.| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAACCTG------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 15/47 (31.9%) +# Similarity: 15/47 (31.9%) +# Gaps: 25/47 (53.2%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAG-GTTCAGAG--TTCTACAGTCCGA 44 + . |.||.|.|| | ||||| ..| |.| +FC12044_91407 1 ------------G-CTCCAATAGCG--CAGAGGAAAC--CTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 13/39 (33.3%) +# Similarity: 13/39 (33.3%) +# Gaps: 21/39 (53.8%) +# Score: 39.5 +# +# +#======================================= + +Illumina_Smal 1 ----CGACAG-GTTCAGAG--TTCTACAGTCCGACGATC 32 + |.|.|| | ||||| ..| |.| +FC12044_91407 1 GCTCCAATAGCG--CAGAGGAAAC--CTG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 8/50 (16.0%) +# Similarity: 8/50 (16.0%) +# Gaps: 42/50 (84.0%) +# Score: 47.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG----------------- 33 + | ||||||| +FC12044_91407 1 ---------------------G----CTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 10/45 (22.2%) +# Similarity: 10/45 (22.2%) +# Gaps: 32/45 (71.1%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT---------- 33 + ||| |||..|| .|| +FC12044_91407 1 --------------------GCTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 70 +# Identity: 10/70 (14.3%) +# Similarity: 10/70 (14.3%) +# Gaps: 57/70 (81.4%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT-C 49 + ||| | +FC12044_91407 1 ---------------------------------------------GCTGC 5 + +Illumina_Geno 50 TTCCGA-TCT---------- 58 + ||..|| .|| +FC12044_91407 6 TTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 27/43 (62.8%) +# Score: 47.0 +# +# +#======================================= + +Illumina_Geno 1 ----C-----AAGCAGAAGACGGCATACGAGCTCTTCCGATCT 34 + | |.||.|| ||||. ||| +FC12044_91407 1 GCTGCTTGGGAGGCTGA----GGCAG--GAG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 10/45 (22.2%) +# Similarity: 10/45 (22.2%) +# Gaps: 32/45 (71.1%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT---------- 33 + ||| |||..|| .|| +FC12044_91407 1 --------------------GCTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 15/35 (42.9%) +# Similarity: 15/35 (42.9%) +# Gaps: 13/35 (37.1%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Pair 1 ---GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG 32 + |.|.||.|| ||.|. .||||||. +FC12044_91407 1 GCTGCTTGGGAG-GCTGA--GGCAGGAG------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 10/45 (22.2%) +# Similarity: 10/45 (22.2%) +# Gaps: 32/45 (71.1%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT---------- 33 + ||| |||..|| .|| +FC12044_91407 1 --------------------GCTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 70 +# Identity: 10/70 (14.3%) +# Similarity: 10/70 (14.3%) +# Gaps: 57/70 (81.4%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT-C 49 + ||| | +FC12044_91407 1 ---------------------------------------------GCTGC 5 + +Illumina_Pair 50 TTCCGA-TCT---------- 58 + ||..|| .|| +FC12044_91407 6 TTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 70 +# Identity: 13/70 (18.6%) +# Similarity: 13/70 (18.6%) +# Gaps: 54/70 (77.1%) +# Score: 47.0 +# +# +#======================================= + +Illumina_Pair 1 ----C-----AAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTG 41 + | |.||.|| ||||. ||| +FC12044_91407 1 GCTGCTTGGGAGGCTGA----GGCAG--GAG------------------- 25 + +Illumina_Pair 42 CTGAACCGCTCTTCCGATCT 61 + +FC12044_91407 26 -------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 10/45 (22.2%) +# Similarity: 10/45 (22.2%) +# Gaps: 32/45 (71.1%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT---------- 33 + ||| |||..|| .|| +FC12044_91407 1 --------------------GCTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 13/45 (28.9%) +# Similarity: 13/45 (28.9%) +# Gaps: 28/45 (62.2%) +# Score: 38.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCT---GAACCGCTCTTCCGA-TCT---- 37 + . ||||| ||. ||| || .|. +FC12044_91407 1 ----------G---CTGCTTGGGAG--GCT-----GAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 10/39 (25.6%) +# Similarity: 10/39 (25.6%) +# Gaps: 27/39 (69.2%) +# Score: 40.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAACT-------CTGA-AC----- 26 + .||| || |||| .| +FC12044_91407 1 ---------GCTG-----CTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 13/32 (40.6%) +# Similarity: 13/32 (40.6%) +# Gaps: 13/32 (40.6%) +# Score: 33.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTT------CAGAGTTCTACAGTCCGAC 26 + .|.|.|| |.||| .||| ||. +FC12044_91407 1 GCTGCTTGGGAGGCTGAG----GCAG---GAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 8/36 (22.2%) +# Similarity: 8/36 (22.2%) +# Gaps: 26/36 (72.2%) +# Score: 41.5 +# +# +#======================================= + +Illumina_DpnI 1 -------------C--AAGCAGAAGACGGCATACGA 21 + | |.||||.|| +FC12044_91407 1 GCTGCTTGGGAGGCTGAGGCAGGAG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 8/38 (21.1%) +# Similarity: 8/38 (21.1%) +# Gaps: 30/38 (78.9%) +# Score: 47.5 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGCCGTCTTCTGCTTG----------------- 21 + | ||||||| +FC12044_91407 1 ---------G----CTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 8/36 (22.2%) +# Similarity: 8/36 (22.2%) +# Gaps: 26/36 (72.2%) +# Score: 41.5 +# +# +#======================================= + +Illumina_DpnI 1 -------------C--AAGCAGAAGACGGCATACGA 21 + | |.||||.|| +FC12044_91407 1 GCTGCTTGGGAGGCTGAGGCAGGAG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 15/51 (29.4%) +# Similarity: 15/51 (29.4%) +# Gaps: 33/51 (64.7%) +# Score: 47.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCG 43 + |.|.| ||| || |.|||| || +FC12044_91407 1 ---GCTGCTTGGGAGGCTGA-----GGCAGG---AG-------------- 25 + +Illumina_DpnI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 19/38 (50.0%) +# Score: 40.0 +# +# +#======================================= + +Illumina_DpnI 1 -CGAC-----AGGTTCAGAGTTCTACAGTCCGACGATC 32 + |..| ||| |.||| .||| || | +FC12044_91407 1 GCTGCTTGGGAGG--CTGAG----GCAG---GA-G--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 40.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAACT-------CTGA-AC----- 21 + .||| || |||| .| +FC12044_91407 1 ----GCTG-----CTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 14/35 (40.0%) +# Similarity: 14/35 (40.0%) +# Gaps: 16/35 (45.7%) +# Score: 37.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTT------CAGAGTTCTACAGTCCGACATG 29 + .|.|.|| |.||| .||| || | +FC12044_91407 1 GCTGCTTGGGAGGCTGAG----GCAG---GA---G 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 8/38 (21.1%) +# Similarity: 8/38 (21.1%) +# Gaps: 28/38 (73.7%) +# Score: 41.5 +# +# +#======================================= + +Illumina_NlaI 1 -------------C--AAGCAGAAGACGGCATACGANN 23 + | |.||||.|| +FC12044_91407 1 GCTGCTTGGGAGGCTGAGGCAGGAG------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 8/38 (21.1%) +# Similarity: 8/38 (21.1%) +# Gaps: 30/38 (78.9%) +# Score: 47.5 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGCCGTCTTCTGCTTG----------------- 21 + | ||||||| +FC12044_91407 1 ---------G----CTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 8/36 (22.2%) +# Similarity: 8/36 (22.2%) +# Gaps: 26/36 (72.2%) +# Score: 41.5 +# +# +#======================================= + +Illumina_NlaI 1 -------------C--AAGCAGAAGACGGCATACGA 21 + | |.||||.|| +FC12044_91407 1 GCTGCTTGGGAGGCTGAGGCAGGAG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 15/51 (29.4%) +# Similarity: 15/51 (29.4%) +# Gaps: 33/51 (64.7%) +# Score: 47.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCG 43 + |.|.| ||| || |.|||| || +FC12044_91407 1 ---GCTGCTTGGGAGGCTGA-----GGCAGG---AG-------------- 25 + +Illumina_NlaI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 14/38 (36.8%) +# Similarity: 14/38 (36.8%) +# Gaps: 19/38 (50.0%) +# Score: 37.0 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTT------CAGAGTTCTACAGTCCGACATG 32 + .|.|.|| |.||| .||| || | +FC12044_91407 1 ---GCTGCTTGGGAGGCTGAG----GCAG---GA---G 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 8/36 (22.2%) +# Similarity: 8/36 (22.2%) +# Gaps: 26/36 (72.2%) +# Score: 41.5 +# +# +#======================================= + +Illumina_Smal 1 -------------C--AAGCAGAAGACGGCATACGA 21 + | |.||||.|| +FC12044_91407 1 GCTGCTTGGGAGGCTGAGGCAGGAG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 30.0 +# +# +#======================================= + +Illumina_Smal 1 ---G-TT------CAGAGTTCTACAGTCCGACGATC 26 + | || |.||| .||| || | +FC12044_91407 1 GCTGCTTGGGAGGCTGAG----GCAG---GA-G--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 9/38 (23.7%) +# Similarity: 9/38 (23.7%) +# Gaps: 29/38 (76.3%) +# Score: 42.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGCCGTCTTCTGCTTG------T---------- 22 + | ||||||| | +FC12044_91407 1 ---------G----CTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 8/36 (22.2%) +# Similarity: 8/36 (22.2%) +# Gaps: 26/36 (72.2%) +# Score: 41.5 +# +# +#======================================= + +Illumina_Smal 1 -------------C--AAGCAGAAGACGGCATACGA 21 + | |.||||.|| +FC12044_91407 1 GCTGCTTGGGAGGCTGAGGCAGGAG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 15/51 (29.4%) +# Similarity: 15/51 (29.4%) +# Gaps: 33/51 (64.7%) +# Score: 47.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCG 43 + |.|.| ||| || |.|||| || +FC12044_91407 1 ---GCTGCTTGGGAGGCTGA-----GGCAGG---AG-------------- 25 + +Illumina_Smal 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 19/38 (50.0%) +# Score: 40.0 +# +# +#======================================= + +Illumina_Smal 1 -CGAC-----AGGTTCAGAGTTCTACAGTCCGACGATC 32 + |..| ||| |.||| .||| || | +FC12044_91407 1 GCTGCTTGGGAGG--CTGAG----GCAG---GA-G--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 13/42 (31.0%) +# Similarity: 13/42 (31.0%) +# Gaps: 26/42 (61.9%) +# Score: 52.0 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGT---ATGC------CGTCTTCTGCTTG 33 + |||.|| | ||.| ||.|| +FC12044_91407 1 -------AGACCT--TTGGATACAATGAACGACT-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 15/43 (34.9%) +# Similarity: 15/43 (34.9%) +# Gaps: 28/43 (65.1%) +# Score: 59.5 +# +# +#======================================= + +Illumina_Geno 1 --ACACTCTTTCCC---TAC-----ACGACGCTCTTCCGATCT 33 + || |||| ||| |||| || +FC12044_91407 1 AGAC---CTTT---GGATACAATGAACGA--CT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 17/66 (25.8%) +# Similarity: 17/66 (25.8%) +# Gaps: 49/66 (74.2%) +# Score: 60.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCC---TAC-----AC 42 + ||| | |||| ||| || +FC12044_91407 1 -------------------AGA-C------CTTT---GGATACAATGAAC 21 + +Illumina_Geno 43 GACGCTCTTCCGATCT 58 + || || +FC12044_91407 22 GA--CT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 17/41 (41.5%) +# Similarity: 17/41 (41.5%) +# Gaps: 23/41 (56.1%) +# Score: 63.0 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGAC----GGCATAC---GAGCTCTTCCGATCT 34 + |||| || |||| ||. ||| || +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAA------CGA-CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 15/43 (34.9%) +# Similarity: 15/43 (34.9%) +# Gaps: 28/43 (65.1%) +# Score: 59.5 +# +# +#======================================= + +Illumina_Geno 1 --ACACTCTTTCCC---TAC-----ACGACGCTCTTCCGATCT 33 + || |||| ||| |||| || +FC12044_91407 1 AGAC---CTTT---GGATACAATGAACGA--CT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 15/37 (40.5%) +# Similarity: 15/37 (40.5%) +# Gaps: 17/37 (45.9%) +# Score: 43.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGC----GGTTCAGCAGGAATGCCGAG- 32 + |||.| |.|.|| |.||| |||. +FC12044_91407 1 -------AGACCTTTGGATACA--ATGAA---CGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 15/43 (34.9%) +# Similarity: 15/43 (34.9%) +# Gaps: 28/43 (65.1%) +# Score: 59.5 +# +# +#======================================= + +Illumina_Pair 1 --ACACTCTTTCCC---TAC-----ACGACGCTCTTCCGATCT 33 + || |||| ||| |||| || +FC12044_91407 1 AGAC---CTTT---GGATACAATGAACGA--CT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 17/66 (25.8%) +# Similarity: 17/66 (25.8%) +# Gaps: 49/66 (74.2%) +# Score: 60.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCC---TAC-----AC 42 + ||| | |||| ||| || +FC12044_91407 1 -------------------AGA-C------CTTT---GGATACAATGAAC 21 + +Illumina_Pair 43 GACGCTCTTCCGATCT 58 + || || +FC12044_91407 22 GA--CT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 17/66 (25.8%) +# Similarity: 17/66 (25.8%) +# Gaps: 46/66 (69.7%) +# Score: 74.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGAC----GGCATACGA-GATCGGTCTCGGCATTCCTGCTGA 45 + |||| || ||||.| ||.| |.|| +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAAC-GACT--------------- 25 + +Illumina_Pair 46 ACCGCTCTTCCGATCT 61 + +FC12044_91407 26 ---------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 15/43 (34.9%) +# Similarity: 15/43 (34.9%) +# Gaps: 28/43 (65.1%) +# Score: 59.5 +# +# +#======================================= + +Illumina_Pair 1 --ACACTCTTTCCC---TAC-----ACGACGCTCTTCCGATCT 33 + || |||| ||| |||| || +FC12044_91407 1 AGAC---CTTT---GGATACAATGAACGA--CT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 12/45 (26.7%) +# Similarity: 12/45 (26.7%) +# Gaps: 28/45 (62.2%) +# Score: 52.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCC------TGC--TGAACCGCTCTTCCGATCT 37 + |..|| |.| |||||..|| +FC12044_91407 1 ----------AGACCTTTGGATACAATGAACGACT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 14/32 (43.8%) +# Similarity: 14/32 (43.8%) +# Gaps: 13/32 (40.6%) +# Score: 62.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGA-CTGTAGA-ACTCTGAAC---- 26 + .|| ||.|.|| ||..||||| +FC12044_91407 1 -------AGACCTTTGGATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 53.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTC-------TACA--GTCCGAC- 26 + ||| | |||| |..|||| +FC12044_91407 1 --------AGA---CCTTTGGATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 22/34 (64.7%) +# Score: 51.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGAC----GGCATACGA--------- 21 + |||| || ||||.| +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 39.5 +# +# +#======================================= + +Illumina_DpnI 1 ------TCGTATGC------CGTCTTCTGCTTG 21 + |.|.||.| ||.|| +FC12044_91407 1 AGACCTTTGGATACAATGAACGACT-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 22/34 (64.7%) +# Score: 51.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGAC----GGCATACGA--------- 21 + |||| || ||||.| +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 16/48 (33.3%) +# Similarity: 16/48 (33.3%) +# Gaps: 27/48 (56.2%) +# Score: 56.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACA--GTCCGA-- 44 + . |||| ||..|| |||| |..||| +FC12044_91407 1 ---------A-GACC---------TTTGGA----TACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 25/41 (61.0%) +# Score: 51.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTC-------TACAGTCCG-ACGATC- 32 + ||| | ||||.| | |||| | +FC12044_91407 1 ----------AGA---CCTTTGGATACAAT--GAACGA-CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 62.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGA-CTGTAGA-ACTCTGAAC---- 21 + .|| ||.|.|| ||..||||| +FC12044_91407 1 --AGACCTTTGGATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 13/38 (34.2%) +# Similarity: 13/38 (34.2%) +# Gaps: 22/38 (57.9%) +# Score: 52.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTC-------TACA--GTCCGACATG 29 + ||| | |||| |..||||. +FC12044_91407 1 --------AGA---CCTTTGGATACAATGAACGACT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 20/34 (58.8%) +# Score: 46.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGAC----GGCATACGANN------- 23 + |||| || ||||.|.. +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 39.5 +# +# +#======================================= + +Illumina_NlaI 1 ------TCGTATGC------CGTCTTCTGCTTG 21 + |.|.||.| ||.|| +FC12044_91407 1 AGACCTTTGGATACAATGAACGACT-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 22/34 (64.7%) +# Score: 51.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGAC----GGCATACGA--------- 21 + |||| || ||||.| +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 16/48 (33.3%) +# Similarity: 16/48 (33.3%) +# Gaps: 27/48 (56.2%) +# Score: 56.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACA--GTCCGA-- 44 + . |||| ||..|| |||| |..||| +FC12044_91407 1 ---------A-GACC---------TTTGGA----TACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 15/37 (40.5%) +# Similarity: 15/37 (40.5%) +# Gaps: 17/37 (45.9%) +# Score: 60.5 +# +# +#======================================= + +Illumina_NlaI 1 ---CCGACAGGTTCAGAGTTCTACA--GTCCGACATG 32 + || ||..|| |||| |..||||. +FC12044_91407 1 AGACC------TTTGGA----TACAATGAACGACT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 22/34 (64.7%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGAC----GGCATACGA--------- 21 + |||| || ||||.| +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 15/35 (42.9%) +# Similarity: 15/35 (42.9%) +# Gaps: 19/35 (54.3%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTC-------TACAGTCCG-ACGATC- 26 + ||| | ||||.| | |||| | +FC12044_91407 1 ----AGA---CCTTTGGATACAAT--GAACGA-CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 21/34 (61.8%) +# Score: 39.5 +# +# +#======================================= + +Illumina_Smal 1 ------TCGTATGC------CGTCTTCTGCTTGT 22 + |.|.||.| ||.|| +FC12044_91407 1 AGACCTTTGGATACAATGAACGACT--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 22/34 (64.7%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGAC----GGCATACGA--------- 21 + |||| || ||||.| +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 16/48 (33.3%) +# Similarity: 16/48 (33.3%) +# Gaps: 27/48 (56.2%) +# Score: 56.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACA--GTCCGA-- 44 + . |||| ||..|| |||| |..||| +FC12044_91407 1 ---------A-GACC---------TTTGGA----TACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 25/41 (61.0%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTC-------TACAGTCCG-ACGATC- 32 + ||| | ||||.| | |||| | +FC12044_91407 1 ----------AGA---CCTTTGGATACAAT--GAACGA-CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 12/44 (27.3%) +# Similarity: 12/44 (27.3%) +# Gaps: 30/44 (68.2%) +# Score: 58.0 +# +# +#======================================= + +Illumina_Geno 1 -----------GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 33 + || |.||.|| ||||| +FC12044_91407 1 AGGGAATTTTAGA---GGAGGGC-----TGCCG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Geno 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + | ||| ||.|.|| |..||.||| +FC12044_91407 1 AGGGAA-----TTT---TAGAGGA-GGGCTGCCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 15/62 (24.2%) +# Similarity: 15/62 (24.2%) +# Gaps: 41/62 (66.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Geno 1 ----AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG 46 + |||..|| |.| |||..||.| || +FC12044_91407 1 AGGGAATTTTA--GAG------GAGGGCTGC--------------CG--- 25 + +Illumina_Geno 47 CTCTTCCGATCT 58 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 19/39 (48.7%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCAT-----ACGAGCTCTTCCGATCT 34 + || ||.|| |.|||..||.||| +FC12044_91407 1 --------AG--GGAATTTTAGAGGAGGGCTGCCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Geno 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + | ||| ||.|.|| |..||.||| +FC12044_91407 1 AGGGAA-----TTT---TAGAGGA-GGGCTGCCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 17/38 (44.7%) +# Similarity: 17/38 (44.7%) +# Gaps: 19/38 (50.0%) +# Score: 61.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGG---TTCAGCAGGAA---TGCCGAG 32 + || || ||.|| ||||. ||||| +FC12044_91407 1 ---------AG-GGAATTTTAG-AGGAGGGCTGCCG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Pair 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + | ||| ||.|.|| |..||.||| +FC12044_91407 1 AGGGAA-----TTT---TAGAGGA-GGGCTGCCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 15/62 (24.2%) +# Similarity: 15/62 (24.2%) +# Gaps: 41/62 (66.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Pair 1 ----AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG 46 + |||..|| |.| |||..||.| || +FC12044_91407 1 AGGGAATTTTA--GAG------GAGGGCTGC--------------CG--- 25 + +Illumina_Pair 47 CTCTTCCGATCT 58 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 20/61 (32.8%) +# Similarity: 20/61 (32.8%) +# Gaps: 36/61 (59.0%) +# Score: 56.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGC 50 + || ||.||...||| ||. || |||| ||| +FC12044_91407 1 --------AG--GGAATTTTAGA--GGA---GG-------GCTG--CCG- 25 + +Illumina_Pair 51 TCTTCCGATCT 61 + +FC12044_91407 26 ----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Pair 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + | ||| ||.|.|| |..||.||| +FC12044_91407 1 AGGGAA-----TTT---TAGAGGA-GGGCTGCCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 14/46 (30.4%) +# Similarity: 14/46 (30.4%) +# Gaps: 30/46 (65.2%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCT---------GCTGAACCGCTCTTCCGATCT 37 + .| ||.||| | |||| ||| +FC12044_91407 1 -AG----GGAATT--TTAGAGGAGGGCTG--CCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 9/41 (22.0%) +# Similarity: 9/41 (22.0%) +# Gaps: 31/41 (75.6%) +# Score: 28.5 +# +# +#======================================= + +Illumina_DpnI 1 --------------GATCGTCGGACTGTAGAACTCTGAAC- 26 + || ||.||| | | +FC12044_91407 1 AGGGAATTTTAGAGGA-----GGGCTG-----C------CG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 14/34 (41.2%) +# Similarity: 14/34 (41.2%) +# Gaps: 17/34 (50.0%) +# Score: 53.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTA--------CAGTCCGAC 26 + ||| ||.||.|| |.| ||| +FC12044_91407 1 --AGG----GAATTTTAGAGGAGGGCTG-CCG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 10/28 (35.7%) +# Score: 35.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAG-------CAGAAGACGGCATACGA 21 + || .|||.||.|||...|| +FC12044_91407 1 --AGGGAATTTTAGAGGAGGGCTGCCG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 8/38 (21.1%) +# Similarity: 8/38 (21.1%) +# Gaps: 30/38 (78.9%) +# Score: 34.5 +# +# +#======================================= + +Illumina_DpnI 1 --------TCGTA---------TGCCGTCTTCTGCTTG 21 + | || ||||| +FC12044_91407 1 AGGGAATTT--TAGAGGAGGGCTGCCG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 10/28 (35.7%) +# Score: 35.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAG-------CAGAAGACGGCATACGA 21 + || .|||.||.|||...|| +FC12044_91407 1 --AGGGAATTTTAGAGGAGGGCTGCCG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 52 +# Identity: 14/52 (26.9%) +# Similarity: 14/52 (26.9%) +# Gaps: 35/52 (67.3%) +# Score: 53.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTA--------CAGTCC 42 + ||| ||.||.|| |.| || +FC12044_91407 1 ---------------------AGG----GAATTTTAGAGGAGGGCTG-CC 24 + +Illumina_DpnI 43 GA 44 + | +FC12044_91407 25 G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 23/40 (57.5%) +# Score: 53.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTA--------CAGTCCGACGATC 32 + ||| ||.||.|| |.| ||| +FC12044_91407 1 ----AGG----GAATTTTAGAGGAGGGCTG-CCG------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 22.5 +# +# +#======================================= + +Illumina_NlaI 1 ------TCGGACTGTAGA-----ACT-CTGAAC- 21 + | |.|||| .|| | | +FC12044_91407 1 AGGGAAT-----TTTAGAGGAGGGCTGC----CG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 14/37 (37.8%) +# Similarity: 14/37 (37.8%) +# Gaps: 20/37 (54.1%) +# Score: 53.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTA--------CAGTCCGACATG 29 + ||| ||.||.|| |.| ||| +FC12044_91407 1 --AGG----GAATTTTAGAGGAGGGCTG-CCG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 12/30 (40.0%) +# Similarity: 12/30 (40.0%) +# Gaps: 12/30 (40.0%) +# Score: 35.0 +# +# +#======================================= + +Illumina_NlaI 1 CAAG-------CAGAAGACGGCATACGANN 23 + || .|||.||.|||...|| +FC12044_91407 1 --AGGGAATTTTAGAGGAGGGCTGCCG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 8/38 (21.1%) +# Similarity: 8/38 (21.1%) +# Gaps: 30/38 (78.9%) +# Score: 34.5 +# +# +#======================================= + +Illumina_NlaI 1 --------TCGTA---------TGCCGTCTTCTGCTTG 21 + | || ||||| +FC12044_91407 1 AGGGAATTT--TAGAGGAGGGCTGCCG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 10/28 (35.7%) +# Score: 35.0 +# +# +#======================================= + +Illumina_NlaI 1 CAAG-------CAGAAGACGGCATACGA 21 + || .|||.||.|||...|| +FC12044_91407 1 --AGGGAATTTTAGAGGAGGGCTGCCG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 52 +# Identity: 14/52 (26.9%) +# Similarity: 14/52 (26.9%) +# Gaps: 35/52 (67.3%) +# Score: 53.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTA--------CAGTCC 42 + ||| ||.||.|| |.| || +FC12044_91407 1 ---------------------AGG----GAATTTTAGAGGAGGGCTG-CC 24 + +Illumina_NlaI 43 GA 44 + | +FC12044_91407 25 G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 23/40 (57.5%) +# Score: 53.0 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGAGTTCTA--------CAGTCCGACATG 32 + ||| ||.||.|| |.| ||| +FC12044_91407 1 -----AGG----GAATTTTAGAGGAGGGCTG-CCG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 10/28 (35.7%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Smal 1 CAAG-------CAGAAGACGGCATACGA 21 + || .|||.||.|||...|| +FC12044_91407 1 --AGGGAATTTTAGAGGAGGGCTGCCG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 12/36 (33.3%) +# Similarity: 12/36 (33.3%) +# Gaps: 21/36 (58.3%) +# Score: 42.0 +# +# +#======================================= + +Illumina_Smal 1 GTTCA--GAGTTCTA--------CAGTCCGACGATC 26 + | ||.||.|| |.| ||| +FC12044_91407 1 ----AGGGAATTTTAGAGGAGGGCTG-CCG------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 8/39 (20.5%) +# Similarity: 8/39 (20.5%) +# Gaps: 31/39 (79.5%) +# Score: 34.5 +# +# +#======================================= + +Illumina_Smal 1 --------TCGTA---------TGCCGTCTTCTGCTTGT 22 + | || ||||| +FC12044_91407 1 AGGGAATTT--TAGAGGAGGGCTGCCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 10/28 (35.7%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Smal 1 CAAG-------CAGAAGACGGCATACGA 21 + || .|||.||.|||...|| +FC12044_91407 1 --AGGGAATTTTAGAGGAGGGCTGCCG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 52 +# Identity: 14/52 (26.9%) +# Similarity: 14/52 (26.9%) +# Gaps: 35/52 (67.3%) +# Score: 53.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTA--------CAGTCC 42 + ||| ||.||.|| |.| || +FC12044_91407 1 ---------------------AGG----GAATTTTAGAGGAGGGCTG-CC 24 + +Illumina_Smal 43 GA 44 + | +FC12044_91407 25 G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 23/40 (57.5%) +# Score: 53.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTA--------CAGTCCGACGATC 32 + ||| ||.||.|| |.| ||| +FC12044_91407 1 ----AGG----GAATTTTAGAGGAGGGCTG-CCG------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 28/43 (65.1%) +# Score: 50.0 +# +# +#======================================= + +Illumina_Geno 1 ----------GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 33 + |.||.| ||| | ||| || +FC12044_91407 1 TCTCCATGTTGGTCAG----GCT-G------GTC-TC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 20/39 (51.3%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCT-----ACACGACGCTCTTCCGATCT- 33 + || |||.| .|| | ||| |.||| +FC12044_91407 1 ----TC--TCCATGTTGGTCA-G--GCT-----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 18/61 (29.5%) +# Similarity: 18/61 (29.5%) +# Gaps: 39/61 (63.9%) +# Score: 65.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTT--TCCCTACACGACGCT 48 + |||.|| |.|| || |.||| +FC12044_91407 1 ----------------------TCTCCA-TGTTGGTC--------AGGCT 19 + +Illumina_Geno 49 CTTCCGATCT- 58 + |.||| +FC12044_91407 20 -----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 13/44 (29.5%) +# Similarity: 13/44 (29.5%) +# Gaps: 29/44 (65.9%) +# Score: 53.5 +# +# +#======================================= + +Illumina_Geno 1 ----CAAG-----CAGAAGACGGCATACGAGCTCTTCCGATCT- 34 + ||.| ||| ||| |.||| +FC12044_91407 1 TCTCCATGTTGGTCAG--------------GCT-----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 20/39 (51.3%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCT-----ACACGACGCTCTTCCGATCT- 33 + || |||.| .|| | ||| |.||| +FC12044_91407 1 ----TC--TCCATGTTGGTCA-G--GCT-----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 11/44 (25.0%) +# Similarity: 11/44 (25.0%) +# Gaps: 31/44 (70.5%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Pair 1 ----------GATCGGAAGAGC-GGT-TCAGCAGGAATGCCGAG 32 + |.||.| || ||| || +FC12044_91407 1 TCTCCATGTTGGTCAG----GCTGGTCTC--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 20/39 (51.3%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCT-----ACACGACGCTCTTCCGATCT- 33 + || |||.| .|| | ||| |.||| +FC12044_91407 1 ----TC--TCCATGTTGGTCA-G--GCT-----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 18/61 (29.5%) +# Similarity: 18/61 (29.5%) +# Gaps: 39/61 (63.9%) +# Score: 65.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTT--TCCCTACACGACGCT 48 + |||.|| |.|| || |.||| +FC12044_91407 1 ----------------------TCTCCA-TGTTGGTC--------AGGCT 19 + +Illumina_Pair 49 CTTCCGATCT- 58 + |.||| +FC12044_91407 20 -----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 16/66 (24.2%) +# Similarity: 16/66 (24.2%) +# Gaps: 46/66 (69.7%) +# Score: 81.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCAT-----TCCTGCTGA 45 + |||| ||| ||..||||. +FC12044_91407 1 ---------------------------TCTC--CATGTTGGTCAGGCTGG 21 + +Illumina_Pair 46 ACCGCTCTTCCGATCT 61 + . ||| +FC12044_91407 22 T---CTC--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 20/39 (51.3%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCT-----ACACGACGCTCTTCCGATCT- 33 + || |||.| .|| | ||| |.||| +FC12044_91407 1 ----TC--TCCATGTTGGTCA-G--GCT-----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 16/42 (38.1%) +# Similarity: 16/42 (38.1%) +# Gaps: 22/42 (52.4%) +# Score: 81.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCAT-----TCCTGCTGAACCGCTCTTCCGATCT 37 + |||| ||| ||..||||.. ||| +FC12044_91407 1 ---TCTC--CATGTTGGTCAGGCTGGT---CTC--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 12/33 (36.4%) +# Similarity: 12/33 (36.4%) +# Gaps: 15/33 (45.5%) +# Score: 64.0 +# +# +#======================================= + +Illumina_DpnI 1 -------GATCGTCGGACTGTAGAACTCTGAAC 26 + |.|.|||.|.|| |..||| +FC12044_91407 1 TCTCCATGTTGGTCAGGCT---GGTCTC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 10/38 (26.3%) +# Similarity: 10/38 (26.3%) +# Gaps: 25/38 (65.8%) +# Score: 53.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACA-----GTCCGAC------- 26 + |||.|| |||.|.| +FC12044_91407 1 -------------TCTCCATGTTGGTCAGGCTGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 8/34 (23.5%) +# Similarity: 8/34 (23.5%) +# Gaps: 22/34 (64.7%) +# Score: 36.5 +# +# +#======================================= + +Illumina_DpnI 1 -------------CAAGCAGAAGACGGCATACGA 21 + ||.||.| |.| .| +FC12044_91407 1 TCTCCATGTTGGTCAGGCTG--GTC-----TC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 46.0 +# +# +#======================================= + +Illumina_DpnI 1 --TC---GTATG--CCGTCT--TCTGCTTG 21 + || || || |.|.|| ||| | +FC12044_91407 1 TCTCCATGT-TGGTCAGGCTGGTCT-C--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 8/34 (23.5%) +# Similarity: 8/34 (23.5%) +# Gaps: 22/34 (64.7%) +# Score: 36.5 +# +# +#======================================= + +Illumina_DpnI 1 -------------CAAGCAGAAGACGGCATACGA 21 + ||.||.| |.| .| +FC12044_91407 1 TCTCCATGTTGGTCAGGCTG--GTC-----TC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 14/50 (28.0%) +# Similarity: 14/50 (28.0%) +# Gaps: 31/50 (62.0%) +# Score: 59.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACC------GACAGGTTCAGAGTTCTACAGTCCGA 44 + . |.|| |.|||| |.|. ||| | +FC12044_91407 1 ------------T-CTCCATGTTGGTCAGG--CTGG--TCT-C------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 23/40 (57.5%) +# Score: 64.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACA-----GTCCGAC-GATC-- 32 + |||.|| |||.|.| |.|| +FC12044_91407 1 ---------------TCTCCATGTTGGTCAGGCTGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 10/28 (35.7%) +# Score: 56.5 +# +# +#======================================= + +Illumina_NlaI 1 --TCGGAC-TGTAGAACT--CTGAAC-- 21 + || | |||.|..|. |||..| +FC12044_91407 1 TCTC---CATGTTGGTCAGGCTGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 12/39 (30.8%) +# Similarity: 12/39 (30.8%) +# Gaps: 24/39 (61.5%) +# Score: 56.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACA-----GTCCGACATG----- 29 + |||.|| |||.|.| || +FC12044_91407 1 -------------TCTCCATGTTGGTCAGGC-TGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 8/36 (22.2%) +# Similarity: 8/36 (22.2%) +# Gaps: 24/36 (66.7%) +# Score: 36.5 +# +# +#======================================= + +Illumina_NlaI 1 -------------CAAGCAGAAGACGGCATACGANN 23 + ||.||.| |.| .| +FC12044_91407 1 TCTCCATGTTGGTCAGGCTG--GTC-----TC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 46.0 +# +# +#======================================= + +Illumina_NlaI 1 --TC---GTATG--CCGTCT--TCTGCTTG 21 + || || || |.|.|| ||| | +FC12044_91407 1 TCTCCATGT-TGGTCAGGCTGGTCT-C--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 8/34 (23.5%) +# Similarity: 8/34 (23.5%) +# Gaps: 22/34 (64.7%) +# Score: 36.5 +# +# +#======================================= + +Illumina_NlaI 1 -------------CAAGCAGAAGACGGCATACGA 21 + ||.||.| |.| .| +FC12044_91407 1 TCTCCATGTTGGTCAGGCTG--GTC-----TC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 14/50 (28.0%) +# Similarity: 14/50 (28.0%) +# Gaps: 31/50 (62.0%) +# Score: 59.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACC------GACAGGTTCAGAGTTCTACAGTCCGA 44 + . |.|| |.|||| |.|. ||| | +FC12044_91407 1 ------------T-CTCCATGTTGGTCAGG--CTGG--TCT-C------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 25/41 (61.0%) +# Score: 56.5 +# +# +#======================================= + +Illumina_NlaI 1 ---CC------GACAGGTTCAGAGTTCTACAGTCCGACATG 32 + || |.|||| |.|. ||| | +FC12044_91407 1 TCTCCATGTTGGTCAGG--CTGG--TCT-C----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 8/34 (23.5%) +# Similarity: 8/34 (23.5%) +# Gaps: 22/34 (64.7%) +# Score: 36.5 +# +# +#======================================= + +Illumina_Smal 1 -------------CAAGCAGAAGACGGCATACGA 21 + ||.||.| |.| .| +FC12044_91407 1 TCTCCATGTTGGTCAGGCTG--GTC-----TC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 13/34 (38.2%) +# Similarity: 13/34 (38.2%) +# Gaps: 17/34 (50.0%) +# Score: 64.0 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACA-----GTCCGAC-GATC-- 26 + |||.|| |||.|.| |.|| +FC12044_91407 1 ---------TCTCCATGTTGGTCAGGCTGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 14/29 (48.3%) +# Similarity: 14/29 (48.3%) +# Gaps: 11/29 (37.9%) +# Score: 51.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGCCGTCT---TCT-GCTTGT--- 22 + || | ||.|.| ||. |||.|| +FC12044_91407 1 TC-T---CCATGTTGGTCAGGCTGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 8/34 (23.5%) +# Similarity: 8/34 (23.5%) +# Gaps: 22/34 (64.7%) +# Score: 36.5 +# +# +#======================================= + +Illumina_Smal 1 -------------CAAGCAGAAGACGGCATACGA 21 + ||.||.| |.| .| +FC12044_91407 1 TCTCCATGTTGGTCAGGCTG--GTC-----TC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 14/50 (28.0%) +# Similarity: 14/50 (28.0%) +# Gaps: 31/50 (62.0%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACC------GACAGGTTCAGAGTTCTACAGTCCGA 44 + . |.|| |.|||| |.|. ||| | +FC12044_91407 1 ------------T-CTCCATGTTGGTCAGG--CTGG--TCT-C------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 23/40 (57.5%) +# Score: 64.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACA-----GTCCGAC-GATC-- 32 + |||.|| |||.|.| |.|| +FC12044_91407 1 ---------------TCTCCATGTTGGTCAGGCTGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 20/39 (51.3%) +# Score: 57.0 +# +# +#======================================= + +Illumina_Geno 1 -GA---TCGGAAGAGCTCGTATG--CCGTCTTCTGCTTG 33 + || ||| ||| ||.|.| |||.| +FC12044_91407 1 TGAACGTCG--AGA---CGCAAGGCCCGCC--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 48.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC-----GACGCT---CTTCCGATCT 33 + |..|| |||||. | |||. | +FC12044_91407 1 ------------TGAACGTCGAGACGCAAGGC--CCGC-C- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 68 +# Identity: 13/68 (19.1%) +# Similarity: 13/68 (19.1%) +# Gaps: 53/68 (77.9%) +# Score: 70.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGA----------CCACCGAGATCTACACTCTTTCCCTAC 40 + ||| |||.||| ||.|| +FC12044_91407 1 --TGA-ACGTCGAGACGCAAGGCCCGCC---------------------- 25 + +Illumina_Geno 41 ACGACGCTCTTCCGATCT 58 + +FC12044_91407 26 ------------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 16/38 (42.1%) +# Similarity: 16/38 (42.1%) +# Gaps: 17/38 (44.7%) +# Score: 51.0 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAA-GACGGCATACG---AGCTCTTCCGATCT 34 + .||| |.|| |.||| .|| |||. | +FC12044_91407 1 -----TGAACGTCG--AGACGCAAGGC----CCGC-C- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 48.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC-----GACGCT---CTTCCGATCT 33 + |..|| |||||. | |||. | +FC12044_91407 1 ------------TGAACGTCGAGACGCAAGGC--CCGC-C- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 15/36 (41.7%) +# Score: 56.0 +# +# +#======================================= + +Illumina_Pair 1 -GA---TCGGAAGAGCGGTTCAGCAGGAATGCCGAG 32 + || ||| ||| || || |||...||| +FC12044_91407 1 TGAACGTCG--AGA-CG---CA--AGGCCCGCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 48.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC-----GACGCT---CTTCCGATCT 33 + |..|| |||||. | |||. | +FC12044_91407 1 ------------TGAACGTCGAGACGCAAGGC--CCGC-C- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 68 +# Identity: 13/68 (19.1%) +# Similarity: 13/68 (19.1%) +# Gaps: 53/68 (77.9%) +# Score: 70.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGA----------CCACCGAGATCTACACTCTTTCCCTAC 40 + ||| |||.||| ||.|| +FC12044_91407 1 --TGA-ACGTCGAGACGCAAGGCCCGCC---------------------- 25 + +Illumina_Pair 41 ACGACGCTCTTCCGATCT 58 + +FC12044_91407 26 ------------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 21/61 (34.4%) +# Similarity: 21/61 (34.4%) +# Gaps: 36/61 (59.0%) +# Score: 85.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGC 50 + .|| ||| .||||| || |..||| |||| +FC12044_91407 1 -----TGA--ACG----TCGAGA-CG--CAAGGC------------CCGC 24 + +Illumina_Pair 51 TCTTCCGATCT 61 + | +FC12044_91407 25 -C--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 48.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC-----GACGCT---CTTCCGATCT 33 + |..|| |||||. | |||. | +FC12044_91407 1 ------------TGAACGTCGAGACGCAAGGC--CCGC-C- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 15/45 (33.3%) +# Similarity: 15/45 (33.3%) +# Gaps: 28/45 (62.2%) +# Score: 61.0 +# +# +#======================================= + +Illumina_Pair 1 ----CGGTCTCG----GCATTCCTGCTGAACCGCTCTTCCGATCT 37 + || ||| |||. .|| |||| | +FC12044_91407 1 TGAACG---TCGAGACGCAA---GGC----CCGC-C--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 13/32 (40.6%) +# Similarity: 13/32 (40.6%) +# Gaps: 13/32 (40.6%) +# Score: 62.5 +# +# +#======================================= + +Illumina_DpnI 1 -GATCGTCGGACTGTAGAACTCTGAAC----- 26 + ||.||||| || ||.|....| +FC12044_91407 1 TGAACGTCG------AG-ACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 15/31 (48.4%) +# Similarity: 15/31 (48.4%) +# Gaps: 11/31 (35.5%) +# Score: 48.5 +# +# +#======================================= + +Illumina_DpnI 1 ---ACAGGTTCAGAGTTCTACA--GTCCGAC 26 + ||. || ||| .| .|| |.|||.| +FC12044_91407 1 TGAACG---TC-GAG-AC-GCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 35.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAA-GACGGCATACG-A--------- 21 + .||| |.|| |.||| | +FC12044_91407 1 -----TGAACGTCG--AGACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 38.5 +# +# +#======================================= + +Illumina_DpnI 1 ------TCGT-----ATG--CCGTCTTCTGCTTG 21 + |||. |.| |||.| +FC12044_91407 1 TGAACGTCGAGACGCAAGGCCCGCC--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 35.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAA-GACGGCATACG-A--------- 21 + .||| |.|| |.||| | +FC12044_91407 1 -----TGAACGTCG--AGACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 16/51 (31.4%) +# Similarity: 16/51 (31.4%) +# Gaps: 33/51 (64.7%) +# Score: 71.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGC--GAC-CA----CCGACAGGTTCAGAGTTCTACAGTCCG 43 + ||| |||.| ||| || |||.| +FC12044_91407 1 --TGA-ACGTCGAGACGCAAGGCCCGCC---------------------- 25 + +Illumina_DpnI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 15/40 (37.5%) +# Similarity: 15/40 (37.5%) +# Gaps: 23/40 (57.5%) +# Score: 54.5 +# +# +#======================================= + +Illumina_DpnI 1 ----CGACAGGTTCAGAGTTCTAC---AGTCCGACGATC- 32 + ||.| ||| || ||.|| || | +FC12044_91407 1 TGAACGTC-------GAG----ACGCAAGGCC--CG--CC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 10/35 (28.6%) +# Similarity: 10/35 (28.6%) +# Gaps: 24/35 (68.6%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAAC-TCTGA-AC------------ 21 + .|||| || || || +FC12044_91407 1 ---------TGAACGTC-GAGACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 15/34 (44.1%) +# Similarity: 15/34 (44.1%) +# Gaps: 14/34 (41.2%) +# Score: 48.5 +# +# +#======================================= + +Illumina_NlaI 1 ---ACAGGTTCAGAGTTCTACA--GTCCGACATG 29 + ||. || ||| .| .|| |.|||.| +FC12044_91407 1 TGAACG---TC-GAG-AC-GCAAGGCCCGCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 11/33 (33.3%) +# Similarity: 11/33 (33.3%) +# Gaps: 18/33 (54.5%) +# Score: 35.0 +# +# +#======================================= + +Illumina_NlaI 1 -------CAA---GCAGAAGACGGCATACGANN 23 + |.| ||| |.|.|.|| | +FC12044_91407 1 TGAACGTCGAGACGCA-AGGCCCGC---C---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 38.5 +# +# +#======================================= + +Illumina_NlaI 1 ------TCGT-----ATG--CCGTCTTCTGCTTG 21 + |||. |.| |||.| +FC12044_91407 1 TGAACGTCGAGACGCAAGGCCCGCC--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 35.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAA-GACGGCATACG-A--------- 21 + .||| |.|| |.||| | +FC12044_91407 1 -----TGAACGTCG--AGACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 16/51 (31.4%) +# Similarity: 16/51 (31.4%) +# Gaps: 33/51 (64.7%) +# Score: 71.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGC--GAC-CA----CCGACAGGTTCAGAGTTCTACAGTCCG 43 + ||| |||.| ||| || |||.| +FC12044_91407 1 --TGA-ACGTCGAGACGCAAGGCCCGCC---------------------- 25 + +Illumina_NlaI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 14/37 (37.8%) +# Similarity: 14/37 (37.8%) +# Gaps: 17/37 (45.9%) +# Score: 44.5 +# +# +#======================================= + +Illumina_NlaI 1 ---CCGACAGGTTCAGAGTTCTACA--GTCCGACATG 32 + .||.| ||| .| .|| |.|||.| +FC12044_91407 1 TGAACGTC-------GAG-AC-GCAAGGCCCGCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 35.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAA-GACGGCATACG-A--------- 21 + .||| |.|| |.||| | +FC12044_91407 1 -----TGAACGTCG--AGACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 15/35 (42.9%) +# Similarity: 15/35 (42.9%) +# Gaps: 19/35 (54.3%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Smal 1 -----GTTCAGAGTTCTAC---AGTCCGACGATC- 26 + | || ||| || ||.|| || | +FC12044_91407 1 TGAACG-TC-GAG----ACGCAAGGCC--CG--CC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 23/35 (65.7%) +# Score: 38.5 +# +# +#======================================= + +Illumina_Smal 1 ------TCGT-----ATG--CCGTCTTCTGCTTGT 22 + |||. |.| |||.| +FC12044_91407 1 TGAACGTCGAGACGCAAGGCCCGCC---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 35.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAA-GACGGCATACG-A--------- 21 + .||| |.|| |.||| | +FC12044_91407 1 -----TGAACGTCG--AGACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 16/51 (31.4%) +# Similarity: 16/51 (31.4%) +# Gaps: 33/51 (64.7%) +# Score: 71.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGC--GAC-CA----CCGACAGGTTCAGAGTTCTACAGTCCG 43 + ||| |||.| ||| || |||.| +FC12044_91407 1 --TGA-ACGTCGAGACGCAAGGCCCGCC---------------------- 25 + +Illumina_Smal 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 15/40 (37.5%) +# Similarity: 15/40 (37.5%) +# Gaps: 23/40 (57.5%) +# Score: 54.5 +# +# +#======================================= + +Illumina_Smal 1 ----CGACAGGTTCAGAGTTCTAC---AGTCCGACGATC- 32 + ||.| ||| || ||.|| || | +FC12044_91407 1 TGAACGTC-------GAG----ACGCAAGGCC--CG--CC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 11/44 (25.0%) +# Similarity: 11/44 (25.0%) +# Gaps: 30/44 (68.2%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Geno 1 -----------GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 33 + | |||..|.|| ||| | +FC12044_91407 1 CTGTCCCCACGG--CGGGGGGGC--------------CTG---G 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGAC------GCTCTTCCGATCT-- 33 + ||.|||| ||||.| || || +FC12044_91407 1 -----CTGTCCC--CACGGCGGGGGGGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 15/66 (22.7%) +# Similarity: 15/66 (22.7%) +# Gaps: 49/66 (74.2%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGAC----- 45 + ||.|||| ||||.| +FC12044_91407 1 ------------------------------CTGTCCC--CACGGCGGGGG 18 + +Illumina_Geno 46 -GCTCTTCCGATCT-- 58 + || || +FC12044_91407 19 GGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 12/42 (28.6%) +# Similarity: 12/42 (28.6%) +# Gaps: 25/42 (59.5%) +# Score: 39.0 +# +# +#======================================= + +Illumina_Geno 1 ------CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT-- 34 + | | |||||....|.|| || +FC12044_91407 1 CTGTCCC---C-----ACGGCGGGGGGGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGAC------GCTCTTCCGATCT-- 33 + ||.|||| ||||.| || || +FC12044_91407 1 -----CTGTCCC--CACGGCGGGGGGGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 12/36 (33.3%) +# Similarity: 12/36 (33.3%) +# Gaps: 15/36 (41.7%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGT----TCAGCAGGAATGCCGAG 32 + |.|| .|.||.||...|||..| +FC12044_91407 1 -----------CTGTCCCCACGGCGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGAC------GCTCTTCCGATCT-- 33 + ||.|||| ||||.| || || +FC12044_91407 1 -----CTGTCCC--CACGGCGGGGGGGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 15/66 (22.7%) +# Similarity: 15/66 (22.7%) +# Gaps: 49/66 (74.2%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGAC----- 45 + ||.|||| ||||.| +FC12044_91407 1 ------------------------------CTGTCCC--CACGGCGGGGG 18 + +Illumina_Pair 46 -GCTCTTCCGATCT-- 58 + || || +FC12044_91407 19 GGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 67 +# Identity: 17/67 (25.4%) +# Similarity: 17/67 (25.4%) +# Gaps: 48/67 (71.6%) +# Score: 53.0 +# +# +#======================================= + +Illumina_Pair 1 ------CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTG 44 + | | |||| ||.| || ||| ||| +FC12044_91407 1 CTGTCCC---C-----ACGG----CGGG---GG----GGC-------CTG 24 + +Illumina_Pair 45 AACCGCTCTTCCGATCT 61 + . +FC12044_91407 25 G---------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGAC------GCTCTTCCGATCT-- 33 + ||.|||| ||||.| || || +FC12044_91407 1 -----CTGTCCC--CACGGCGGGGGGGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 13/47 (27.7%) +# Similarity: 13/47 (27.7%) +# Gaps: 32/47 (68.1%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Pair 1 CGGT---CTCGGCATT-------CCTGCTGAACCGCTCTTCCGATCT 37 + |.|| |.|||| |||| | +FC12044_91407 1 CTGTCCCCACGGC---GGGGGGGCCTG--G----------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 13/32 (40.6%) +# Similarity: 13/32 (40.6%) +# Gaps: 13/32 (40.6%) +# Score: 38.0 +# +# +#======================================= + +Illumina_DpnI 1 GATC-GT-----CGGACTGTAGAACTCTGAAC 26 + | || ||| |.|..|..| |||. +FC12044_91407 1 ---CTGTCCCCACGG-CGGGGGGGC-CTGG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 7/42 (16.7%) +# Similarity: 7/42 (16.7%) +# Gaps: 33/42 (78.6%) +# Score: 44.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACAGTC----CGAC------------ 26 + |.||| ||.| +FC12044_91407 1 -----------------CTGTCCCCACGGCGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 11/29 (37.9%) +# Similarity: 11/29 (37.9%) +# Gaps: 12/29 (41.4%) +# Score: 32.5 +# +# +#======================================= + +Illumina_DpnI 1 -------CA-AGCAGAAGACGGCATACGA 21 + || .||.|..| |||.|. | +FC12044_91407 1 CTGTCCCCACGGCGGGGG--GGCCTG-G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 29.0 +# +# +#======================================= + +Illumina_DpnI 1 ---TCGTATGCCGTCTTCTGCTTG----------- 21 + || || | .|.|| | +FC12044_91407 1 CTGTC-----CC--C-ACGGC--GGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 11/29 (37.9%) +# Similarity: 11/29 (37.9%) +# Gaps: 12/29 (41.4%) +# Score: 32.5 +# +# +#======================================= + +Illumina_DpnI 1 -------CA-AGCAGAAGACGGCATACGA 21 + || .||.|..| |||.|. | +FC12044_91407 1 CTGTCCCCACGGCGGGGG--GGCCTG-G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 16/47 (34.0%) +# Similarity: 16/47 (34.0%) +# Gaps: 25/47 (53.2%) +# Score: 53.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGC-GACCACCGACAGGTTCAGAGTTCTACAGTCC--GA 44 + | |.|| || ||.| |.|.| .|.|| | +FC12044_91407 1 ----------CTGTCC-CC-ACGG---CGGGG------GGGCCTGG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 9/45 (20.0%) +# Similarity: 9/45 (20.0%) +# Gaps: 33/45 (73.3%) +# Score: 43.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACAGT-CCGACGATC------------ 32 + |.|| ||.|||. | +FC12044_91407 1 -------------------CTGTCCCCACGG-CGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 10/29 (34.5%) +# Similarity: 10/29 (34.5%) +# Gaps: 12/29 (41.4%) +# Score: 36.0 +# +# +#======================================= + +Illumina_NlaI 1 --------TCGGACTGTAGAACTCTGAAC 21 + .||| |.|..|..| |||. +FC12044_91407 1 CTGTCCCCACGG-CGGGGGGGC-CTGG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 9/42 (21.4%) +# Similarity: 9/42 (21.4%) +# Gaps: 30/42 (71.4%) +# Score: 47.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAGTCC------------GACATG- 29 + |.|||| |.|.|| +FC12044_91407 1 -----------------CTGTCCCCACGGCGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 14/31 (45.2%) +# Score: 32.5 +# +# +#======================================= + +Illumina_NlaI 1 -------CA-AGCAGAAGACGGCATACGANN 23 + || .||.|..| |||.|. | +FC12044_91407 1 CTGTCCCCACGGCGGGGG--GGCCTG-G--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 29.0 +# +# +#======================================= + +Illumina_NlaI 1 ---TCGTATGCCGTCTTCTGCTTG----------- 21 + || || | .|.|| | +FC12044_91407 1 CTGTC-----CC--C-ACGGC--GGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 11/29 (37.9%) +# Similarity: 11/29 (37.9%) +# Gaps: 12/29 (41.4%) +# Score: 32.5 +# +# +#======================================= + +Illumina_NlaI 1 -------CA-AGCAGAAGACGGCATACGA 21 + || .||.|..| |||.|. | +FC12044_91407 1 CTGTCCCCACGGCGGGGG--GGCCTG-G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 16/47 (34.0%) +# Similarity: 16/47 (34.0%) +# Gaps: 25/47 (53.2%) +# Score: 53.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGC-GACCACCGACAGGTTCAGAGTTCTACAGTCC--GA 44 + | |.|| || ||.| |.|.| .|.|| | +FC12044_91407 1 ----------CTGTCC-CC-ACGG---CGGGG------GGGCCTGG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 9/45 (20.0%) +# Similarity: 9/45 (20.0%) +# Gaps: 33/45 (73.3%) +# Score: 47.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGAGTTCTACAGTCC------------GACATG- 32 + |.|||| |.|.|| +FC12044_91407 1 --------------------CTGTCCCCACGGCGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 11/29 (37.9%) +# Similarity: 11/29 (37.9%) +# Gaps: 12/29 (41.4%) +# Score: 32.5 +# +# +#======================================= + +Illumina_Smal 1 -------CA-AGCAGAAGACGGCATACGA 21 + || .||.|..| |||.|. | +FC12044_91407 1 CTGTCCCCACGGCGGGGG--GGCCTG-G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 9/39 (23.1%) +# Similarity: 9/39 (23.1%) +# Gaps: 27/39 (69.2%) +# Score: 43.0 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAGT-CCGACGATC------------ 26 + |.|| ||.|||. | +FC12044_91407 1 -------------CTGTCCCCACGG-CGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 10/31 (32.3%) +# Similarity: 10/31 (32.3%) +# Gaps: 15/31 (48.4%) +# Score: 27.5 +# +# +#======================================= + +Illumina_Smal 1 ---TCGTATGCCGTCTTCTG------CTTGT 22 + || ||..|..|.| ||.| +FC12044_91407 1 CTGTC-----CCCACGGCGGGGGGGCCTGG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 11/29 (37.9%) +# Similarity: 11/29 (37.9%) +# Gaps: 12/29 (41.4%) +# Score: 32.5 +# +# +#======================================= + +Illumina_Smal 1 -------CA-AGCAGAAGACGGCATACGA 21 + || .||.|..| |||.|. | +FC12044_91407 1 CTGTCCCCACGGCGGGGG--GGCCTG-G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 16/47 (34.0%) +# Similarity: 16/47 (34.0%) +# Gaps: 25/47 (53.2%) +# Score: 53.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGC-GACCACCGACAGGTTCAGAGTTCTACAGTCC--GA 44 + | |.|| || ||.| |.|.| .|.|| | +FC12044_91407 1 ----------CTGTCC-CC-ACGG---CGGGG------GGGCCTGG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 9/45 (20.0%) +# Similarity: 9/45 (20.0%) +# Gaps: 33/45 (73.3%) +# Score: 43.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACAGT-CCGACGATC------------ 32 + |.|| ||.|||. | +FC12044_91407 1 -------------------CTGTCCCCACGG-CGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 16/35 (45.7%) +# Similarity: 16/35 (45.7%) +# Gaps: 12/35 (34.3%) +# Score: 79.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTATGCC--GTCTTCTGCTTG 33 + ||.|..|||.||..|| |.||| || +FC12044_91407 1 -----GATGTACTCTTACACCCAGACTT-TG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 76.0 +# +# +#======================================= + +Illumina_Geno 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + ||.|| ||||| ||...|| +FC12044_91407 1 GATGTACTCT-------TACAC---------CCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 17/61 (27.9%) +# Similarity: 17/61 (27.9%) +# Gaps: 39/61 (63.9%) +# Score: 93.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT 50 + |||.|||.|| ||||| +FC12044_91407 1 --------------------GATGTACTCT-------TACAC-------- 15 + +Illumina_Geno 51 TCCGATCT--- 58 + ||...|| +FC12044_91407 16 -CCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 14/41 (34.1%) +# Similarity: 14/41 (34.1%) +# Gaps: 23/41 (56.1%) +# Score: 67.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACGAGCTCTT----CCGATCT--- 34 + ||.| || ||||| ||...|| +FC12044_91407 1 ------GATG------TA----CTCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 76.0 +# +# +#======================================= + +Illumina_Geno 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + ||.|| ||||| ||...|| +FC12044_91407 1 GATGTACTCT-------TACAC---------CCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 12/39 (30.8%) +# Similarity: 12/39 (30.8%) +# Gaps: 21/39 (53.8%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTC-AGCAGGAATGCCGAG------ 32 + ||.|..| || ..|| ||.|| +FC12044_91407 1 -----GATGTAC---TCTTACA------CCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 76.0 +# +# +#======================================= + +Illumina_Pair 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + ||.|| ||||| ||...|| +FC12044_91407 1 GATGTACTCT-------TACAC---------CCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 17/61 (27.9%) +# Similarity: 17/61 (27.9%) +# Gaps: 39/61 (63.9%) +# Score: 93.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT 50 + |||.|||.|| ||||| +FC12044_91407 1 --------------------GATGTACTCT-------TACAC-------- 15 + +Illumina_Pair 51 TCCGATCT--- 58 + ||...|| +FC12044_91407 16 -CCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 65 +# Identity: 19/65 (29.2%) +# Similarity: 19/65 (29.2%) +# Gaps: 44/65 (67.7%) +# Score: 69.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGT-CTCGGCATTCCTGCTGAACCG 49 + ||| || ||| ||.| ||| +FC12044_91407 1 ---------------------GAT--GTACTC----TTAC------ACC- 16 + +Illumina_Pair 50 CTCTTCCGATCT--- 61 + |.|| || +FC12044_91407 17 -----CAGA-CTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 76.0 +# +# +#======================================= + +Illumina_Pair 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + ||.|| ||||| ||...|| +FC12044_91407 1 GATGTACTCT-------TACAC---------CCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 13/44 (29.5%) +# Similarity: 13/44 (29.5%) +# Gaps: 26/44 (59.1%) +# Score: 68.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAACCGCTCTT----CCGATCT--- 37 + |.||.| ||||| ||...|| +FC12044_91407 1 ----------------GATGTA---CTCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 13/37 (35.1%) +# Similarity: 13/37 (35.1%) +# Gaps: 23/37 (62.2%) +# Score: 57.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAACTCTGA-AC---------- 26 + || ||| |||||.| || +FC12044_91407 1 --------GA-TGT---ACTCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 14/36 (38.9%) +# Similarity: 14/36 (38.9%) +# Gaps: 21/36 (58.3%) +# Score: 63.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGA-GTTC---TACAGTCC--GAC---- 26 + || ||.| |||| || ||| +FC12044_91407 1 ---------GATGTACTCTTACA--CCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 39.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAG-ACGGCATAC-----GA----- 21 + ||.| || .|.||| || +FC12044_91407 1 ------GATGTAC-TCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 12/30 (40.0%) +# Similarity: 12/30 (40.0%) +# Gaps: 14/30 (46.7%) +# Score: 55.5 +# +# +#======================================= + +Illumina_DpnI 1 -------TCGTATGCC--GTCTTCTGCTTG 21 + ||.||..|| |.||| || +FC12044_91407 1 GATGTACTCTTACACCCAGACTT-TG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 39.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAG-ACGGCATAC-----GA----- 21 + ||.| || .|.||| || +FC12044_91407 1 ------GATGTAC-TCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 67.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGAC---CACCGACAGGTTCAGAGTTCTACAGTCCGA 44 + .||| ||| .| |||| ||||.|| |. +FC12044_91407 1 GATG-TAC----TCTTACACC--------CAGACTT-TG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 25/41 (61.0%) +# Score: 58.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGA-GTTC---TACAGTCC--GAC---GATC 32 + || ||.| |||| || ||| | +FC12044_91407 1 -----------GATGTACTCTTACA--CCCAGACTTTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 13/32 (40.6%) +# Similarity: 13/32 (40.6%) +# Gaps: 18/32 (56.2%) +# Score: 57.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAACTCTGA-AC---------- 21 + || ||| |||||.| || +FC12044_91407 1 ---GA-TGT---ACTCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 18/36 (50.0%) +# Score: 65.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGA-GTTC---TACAGTCC--GAC-ATG 29 + || ||.| |||| || ||| .|| +FC12044_91407 1 ---------GATGTACTCTTACA--CCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 10/35 (28.6%) +# Similarity: 10/35 (28.6%) +# Gaps: 22/35 (62.9%) +# Score: 30.0 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAG-ACGGCATAC-----------GANN 23 + ||.| || .|.||| | +FC12044_91407 1 ------GATGTAC-TCTTACACCCAGACTTTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 12/30 (40.0%) +# Similarity: 12/30 (40.0%) +# Gaps: 14/30 (46.7%) +# Score: 55.5 +# +# +#======================================= + +Illumina_NlaI 1 -------TCGTATGCC--GTCTTCTGCTTG 21 + ||.||..|| |.||| || +FC12044_91407 1 GATGTACTCTTACACCCAGACTT-TG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 39.0 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAG-ACGGCATAC-----GA----- 21 + ||.| || .|.||| || +FC12044_91407 1 ------GATGTAC-TCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 67.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGAC---CACCGACAGGTTCAGAGTTCTACAGTCCGA 44 + .||| ||| .| |||| ||||.|| |. +FC12044_91407 1 GATG-TAC----TCTTACACC--------CAGACTT-TG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 21/39 (53.8%) +# Score: 65.0 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGA-GTTC---TACAGTCC--GAC-ATG 32 + || ||.| |||| || ||| .|| +FC12044_91407 1 ------------GATGTACTCTTACA--CCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 39.0 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAG-ACGGCATAC-----GA----- 21 + ||.| || .|.||| || +FC12044_91407 1 ------GATGTAC-TCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 15/35 (42.9%) +# Similarity: 15/35 (42.9%) +# Gaps: 19/35 (54.3%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Smal 1 ---GTTCAGAGTTC-TACAGTCC--GAC---GATC 26 + ||.| || |||| || ||| | +FC12044_91407 1 GATGTAC-----TCTTACA--CCCAGACTTTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 15/31 (48.4%) +# Score: 55.5 +# +# +#======================================= + +Illumina_Smal 1 -------TCGTATGCC--GTCTTCTGCTTGT 22 + ||.||..|| |.||| || +FC12044_91407 1 GATGTACTCTTACACCCAGACTT-TG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 39.0 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAG-ACGGCATAC-----GA----- 21 + ||.| || .|.||| || +FC12044_91407 1 ------GATGTAC-TCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 67.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGAC---CACCGACAGGTTCAGAGTTCTACAGTCCGA 44 + .||| ||| .| |||| ||||.|| |. +FC12044_91407 1 GATG-TAC----TCTTACACC--------CAGACTT-TG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 25/41 (61.0%) +# Score: 58.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGA-GTTC---TACAGTCC--GAC---GATC 32 + || ||.| |||| || ||| | +FC12044_91407 1 -----------GATGTACTCTTACA--CCCAGACTTTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 14/42 (33.3%) +# Similarity: 14/42 (33.3%) +# Gaps: 26/42 (61.9%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Geno 1 GATC---GGAAGA-----GCTCGTATGCC-GTCTTCTGCTTG 33 + || ||..|| |||| || || +FC12044_91407 1 --TCAAGGGTGGATCTTGGCTC-----CCAGT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Geno 1 ACA-------CTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| .|||| | |||| ||..| +FC12044_91407 1 TCAAGGGTGGATCTT---------G--GCTC--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 60 +# Identity: 17/60 (28.3%) +# Similarity: 17/60 (28.3%) +# Gaps: 37/60 (61.7%) +# Score: 57.5 +# +# +#======================================= + +Illumina_Geno 1 --AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT 48 + ||.|.| .||||| |. ||| +FC12044_91407 1 TCAAGGGT-------------GGATCT------------TG------GCT 19 + +Illumina_Geno 49 CTTCCGATCT 58 + | ||..| +FC12044_91407 20 C--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 16/35 (45.7%) +# Similarity: 16/35 (45.7%) +# Gaps: 11/35 (31.4%) +# Score: 63.0 +# +# +#======================================= + +Illumina_Geno 1 -CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT 34 + |||| .|..|| .|.| .|||| ||..| +FC12044_91407 1 TCAAG-GGTGGA--TCTT---GGCTC--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Geno 1 ACA-------CTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| .|||| | |||| ||..| +FC12044_91407 1 TCAAGGGTGGATCTT---------G--GCTC--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 16/35 (45.7%) +# Similarity: 16/35 (45.7%) +# Gaps: 13/35 (37.1%) +# Score: 62.5 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCA--GCAGGAATGCCGAG- 32 + || |||.|.||.||. ||. ||.|| +FC12044_91407 1 --TC--AAGGGTGGATCTTGGCT------CCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Pair 1 ACA-------CTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| .|||| | |||| ||..| +FC12044_91407 1 TCAAGGGTGGATCTT---------G--GCTC--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 60 +# Identity: 17/60 (28.3%) +# Similarity: 17/60 (28.3%) +# Gaps: 37/60 (61.7%) +# Score: 57.5 +# +# +#======================================= + +Illumina_Pair 1 --AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT 48 + ||.|.| .||||| |. ||| +FC12044_91407 1 TCAAGGGT-------------GGATCT------------TG------GCT 19 + +Illumina_Pair 49 CTTCCGATCT 58 + | ||..| +FC12044_91407 20 C--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 18/62 (29.0%) +# Similarity: 18/62 (29.0%) +# Gaps: 38/62 (61.3%) +# Score: 74.0 +# +# +#======================================= + +Illumina_Pair 1 -CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCG 49 + |||| |.|.|.|||.||| ||| |.|. +FC12044_91407 1 TCAAG-----------------GGTGGATCTTGGC--TCC--CAGT---- 25 + +Illumina_Pair 50 CTCTTCCGATCT 61 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Pair 1 ACA-------CTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| .|||| | |||| ||..| +FC12044_91407 1 TCAAGGGTGGATCTT---------G--GCTC--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 19/41 (46.3%) +# Similarity: 19/41 (46.3%) +# Gaps: 20/41 (48.8%) +# Score: 65.0 +# +# +#======================================= + +Illumina_Pair 1 -C---GGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT 37 + | ||| || || || || |||| ||..| +FC12044_91407 1 TCAAGGGT---GG-AT--CT--TG----GCTC--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 51.5 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTC--GGACTGTAGAACT---CTGAAC----- 26 + || || ||.||.|| || | +FC12044_91407 1 -----TCAAGG---GTGGATCTTGGCT---CCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 58.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTC-AGAGT---TCTA------CAGTCCGAC 26 + || ||.|| |||. |||| +FC12044_91407 1 ------TCAAGGGTGGATCTTGGCTCCCAGT----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 40.5 +# +# +#======================================= + +Illumina_DpnI 1 -CAAGCAGAAGA-C--GGCATACGA-- 21 + |||| .|..|| | ||| |.|.| +FC12044_91407 1 TCAAG-GGTGGATCTTGGC-TCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 38.5 +# +# +#======================================= + +Illumina_DpnI 1 TC--GTATGCCG-TCTT--CTGCTTG- 21 + || |..|| | |||| ||.|..| +FC12044_91407 1 TCAAGGGTG--GATCTTGGCTCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 40.5 +# +# +#======================================= + +Illumina_DpnI 1 -CAAGCAGAAGA-C--GGCATACGA-- 21 + |||| .|..|| | ||| |.|.| +FC12044_91407 1 TCAAG-GGTGGATCTTGGC-TCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 13/54 (24.1%) +# Similarity: 13/54 (24.1%) +# Gaps: 39/54 (72.2%) +# Score: 58.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTC-AGAGT---TCTA------CAGT 40 + || ||.|| |||. |||| +FC12044_91407 1 -------------------------TCAAGGGTGGATCTTGGCTCCCAGT 25 + +Illumina_DpnI 41 CCGA 44 + +FC12044_91407 26 ---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 19/38 (50.0%) +# Score: 60.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTC-AGAGT---TCTA--CAGTCCGACGATC 32 + || ||.|| |||. | ||| |..| +FC12044_91407 1 --------TCAAGGGTGGATCTTGGC--TCC--CAGT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 13/31 (41.9%) +# Similarity: 13/31 (41.9%) +# Gaps: 16/31 (51.6%) +# Score: 51.5 +# +# +#======================================= + +Illumina_NlaI 1 TC--GGACTGTAGAACT---CTGAAC----- 21 + || || ||.||.|| || | +FC12044_91407 1 TCAAGG---GTGGATCTTGGCT---CCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 18/36 (50.0%) +# Score: 60.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTC-AGAGT---TCTA--CAGTCCGACA-TG 29 + || ||.|| |||. | ||| || | +FC12044_91407 1 ------TCAAGGGTGGATCTTGGC--TCC--CAGT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 14/29 (48.3%) +# Similarity: 14/29 (48.3%) +# Gaps: 10/29 (34.5%) +# Score: 38.0 +# +# +#======================================= + +Illumina_NlaI 1 -CAAGCAGAAGA-C--GGCATAC--GANN 23 + |||| .|..|| | ||| |.| |. +FC12044_91407 1 TCAAG-GGTGGATCTTGGC-TCCCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 38.5 +# +# +#======================================= + +Illumina_NlaI 1 TC--GTATGCCG-TCTT--CTGCTTG- 21 + || |..|| | |||| ||.|..| +FC12044_91407 1 TCAAGGGTG--GATCTTGGCTCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 40.5 +# +# +#======================================= + +Illumina_NlaI 1 -CAAGCAGAAGA-C--GGCATACGA-- 21 + |||| .|..|| | ||| |.|.| +FC12044_91407 1 TCAAG-GGTGGATCTTGGC-TCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 13/54 (24.1%) +# Similarity: 13/54 (24.1%) +# Gaps: 39/54 (72.2%) +# Score: 58.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTC-AGAGT---TCTA------CAGT 40 + || ||.|| |||. |||| +FC12044_91407 1 -------------------------TCAAGGGTGGATCTTGGCTCCCAGT 25 + +Illumina_NlaI 41 CCGA 44 + +FC12044_91407 26 ---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 21/39 (53.8%) +# Score: 60.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTC-AGAGT---TCTA--CAGTCCGACA-TG 32 + || ||.|| |||. | ||| || | +FC12044_91407 1 ---------TCAAGGGTGGATCTTGGC--TCC--CAGT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 40.5 +# +# +#======================================= + +Illumina_Smal 1 -CAAGCAGAAGA-C--GGCATACGA-- 21 + |||| .|..|| | ||| |.|.| +FC12044_91407 1 TCAAG-GGTGGATCTTGGC-TCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 15/32 (46.9%) +# Similarity: 15/32 (46.9%) +# Gaps: 13/32 (40.6%) +# Score: 60.5 +# +# +#======================================= + +Illumina_Smal 1 GTTC-AGAGT---TCTA--CAGTCCGACGATC 26 + || ||.|| |||. | ||| |..| +FC12044_91407 1 --TCAAGGGTGGATCTTGGC--TCC--CAGT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 15/27 (55.6%) +# Similarity: 15/27 (55.6%) +# Gaps: 7/27 (25.9%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Smal 1 TC--GTATGCCG-TCTT--CTGCTTGT 22 + || |..|| | |||| ||.|..|| +FC12044_91407 1 TCAAGGGTG--GATCTTGGCTCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 40.5 +# +# +#======================================= + +Illumina_Smal 1 -CAAGCAGAAGA-C--GGCATACGA-- 21 + |||| .|..|| | ||| |.|.| +FC12044_91407 1 TCAAG-GGTGGATCTTGGC-TCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 13/54 (24.1%) +# Similarity: 13/54 (24.1%) +# Gaps: 39/54 (72.2%) +# Score: 58.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTC-AGAGT---TCTA------CAGT 40 + || ||.|| |||. |||| +FC12044_91407 1 -------------------------TCAAGGGTGGATCTTGGCTCCCAGT 25 + +Illumina_Smal 41 CCGA 44 + +FC12044_91407 26 ---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 19/38 (50.0%) +# Score: 60.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTC-AGAGT---TCTA--CAGTCCGACGATC 32 + || ||.|| |||. | ||| |..| +FC12044_91407 1 --------TCAAGGGTGGATCTTGGC--TCC--CAGT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 17/38 (44.7%) +# Similarity: 17/38 (44.7%) +# Gaps: 18/38 (47.4%) +# Score: 63.5 +# +# +#======================================= + +Illumina_Geno 1 GAT--CGGAAGAGCTCGTATGCCGTCTT---CTGCTTG 33 + | ||..|||| | |.||| |||| | +FC12044_91407 1 --TTGCGACAGAG-T--TTTGC--TCTTGTCC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 28/43 (65.1%) +# Score: 74.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGAC--------GCTCT--TCCGATCT 33 + || .|||| ||||| ||| +FC12044_91407 1 -------TT------GCGACAGAGTTTTGCTCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 59 +# Identity: 20/59 (33.9%) +# Similarity: 20/59 (33.9%) +# Gaps: 35/59 (59.3%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTT-TCCCTACACGACGCTC 49 + |. |||| || |||.|.| .||||| ||| +FC12044_91407 1 -----TT--GCGA-CA--GAGTTTT--GCTCTTGTCC------------- 25 + +Illumina_Geno 50 TTCCGATCT 58 + +FC12044_91407 26 --------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 23/41 (56.1%) +# Score: 69.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACGA-----GCTCT--TCCGATCT 34 + ..| ||.|| || ||||| ||| +FC12044_91407 1 -------TTG-CGACA---GAGTTTTGCTCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 28/43 (65.1%) +# Score: 74.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGAC--------GCTCT--TCCGATCT 33 + || .|||| ||||| ||| +FC12044_91407 1 -------TT------GCGACAGAGTTTTGCTCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 15/35 (42.9%) +# Similarity: 15/35 (42.9%) +# Gaps: 13/35 (37.1%) +# Score: 43.0 +# +# +#======================================= + +Illumina_Pair 1 GAT--CGGAAGAGCGGTTCAGCAGGAATG-CCGAG 32 + | ||..||| |||..||. ..|| || +FC12044_91407 1 --TTGCGACAGA---GTTTTGCT--CTTGTCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 28/43 (65.1%) +# Score: 74.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGAC--------GCTCT--TCCGATCT 33 + || .|||| ||||| ||| +FC12044_91407 1 -------TT------GCGACAGAGTTTTGCTCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 59 +# Identity: 20/59 (33.9%) +# Similarity: 20/59 (33.9%) +# Gaps: 35/59 (59.3%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTT-TCCCTACACGACGCTC 49 + |. |||| || |||.|.| .||||| ||| +FC12044_91407 1 -----TT--GCGA-CA--GAGTTTT--GCTCTTGTCC------------- 25 + +Illumina_Pair 50 TTCCGATCT 58 + +FC12044_91407 26 --------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 63 +# Identity: 19/63 (30.2%) +# Similarity: 19/63 (30.2%) +# Gaps: 40/63 (63.5%) +# Score: 77.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGC 50 + ..| ||.|| |||.| || || +FC12044_91407 1 -------TTG-CGACA---GAGTT-----------TT-----------GC 17 + +Illumina_Pair 51 TCT--TCCGATCT 61 + ||| ||| +FC12044_91407 18 TCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 28/43 (65.1%) +# Score: 74.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGAC--------GCTCT--TCCGATCT 33 + || .|||| ||||| ||| +FC12044_91407 1 -------TT------GCGACAGAGTTTTGCTCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 15/46 (32.6%) +# Similarity: 15/46 (32.6%) +# Gaps: 30/46 (65.2%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCT--CGGCA-----TTCCTGCTGAACCGCTCT--TCCGATCT 37 + | ||.|| || ||||| ||| +FC12044_91407 1 -----TTGCGACAGAGTTTT-----------GCTCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 14/29 (48.3%) +# Similarity: 14/29 (48.3%) +# Gaps: 7/29 (24.1%) +# Score: 60.0 +# +# +#======================================= + +Illumina_DpnI 1 GAT--CGTCGGACTGTAGAACTCTGAAC- 26 + | ||.|.||.|.|.| ||||...| +FC12044_91407 1 --TTGCGACAGAGTTTTG--CTCTTGTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 15/33 (45.5%) +# Similarity: 15/33 (45.5%) +# Gaps: 15/33 (45.5%) +# Score: 78.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTT----CAGAGTTCTAC---AGTCCGAC 26 + || |||||||.|.| .|||| +FC12044_91407 1 -----TTGCGACAGAGTTTTGCTCTTGTCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 31.0 +# +# +#======================================= + +Illumina_DpnI 1 ---CAAGCAGA----------AGACGGCATACGA 21 + |.| |||| .|.| | +FC12044_91407 1 TTGCGA-CAGAGTTTTGCTCTTGTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 56.5 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGC--C---GTCTT-CTGCTTG--- 21 + .||| | ||.|| || |||| +FC12044_91407 1 ----TTGCGACAGAGTTTTGCT-CTTGTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 31.0 +# +# +#======================================= + +Illumina_DpnI 1 ---CAAGCAGA----------AGACGGCATACGA 21 + |.| |||| .|.| | +FC12044_91407 1 TTGCGA-CAGAGTTTTGCTCTTGTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 83.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTAC---AGTCCGA 44 + |. |||| |||||||.|.| .|||| +FC12044_91407 1 -----TT--GCGA-------------CAGAGTTTTGCTCTTGTCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 17/39 (43.6%) +# Similarity: 17/39 (43.6%) +# Gaps: 21/39 (53.8%) +# Score: 86.5 +# +# +#======================================= + +Illumina_DpnI 1 ---CGACAG-GTT---CAGAGTTCTACAGTCCGACGATC 32 + |||||| ||| | ||| .|||| +FC12044_91407 1 TTGCGACAGAGTTTTGC-----TCT--TGTCC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 13/28 (46.4%) +# Similarity: 13/28 (46.4%) +# Gaps: 10/28 (35.7%) +# Score: 49.0 +# +# +#======================================= + +Illumina_NlaI 1 T--CGGACTG----TAGAACTCTGAAC- 21 + | | |||.| |.| ||||...| +FC12044_91407 1 TTGC-GACAGAGTTTTG--CTCTTGTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 15/36 (41.7%) +# Similarity: 15/36 (41.7%) +# Gaps: 18/36 (50.0%) +# Score: 78.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTT----CAGAGTTCTAC---AGTCCGACATG 29 + || |||||||.|.| .|||| +FC12044_91407 1 -----TTGCGACAGAGTTTTGCTCTTGTCC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 9/36 (25.0%) +# Similarity: 9/36 (25.0%) +# Gaps: 24/36 (66.7%) +# Score: 31.0 +# +# +#======================================= + +Illumina_NlaI 1 ---CAAGCAGA----------AGACGGCATACGANN 23 + |.| |||| .|.| | +FC12044_91407 1 TTGCGA-CAGAGTTTTGCTCTTGTC--C-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 56.5 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGC--C---GTCTT-CTGCTTG--- 21 + .||| | ||.|| || |||| +FC12044_91407 1 ----TTGCGACAGAGTTTTGCT-CTTGTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 31.0 +# +# +#======================================= + +Illumina_NlaI 1 ---CAAGCAGA----------AGACGGCATACGA 21 + |.| |||| .|.| | +FC12044_91407 1 TTGCGA-CAGAGTTTTGCTCTTGTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 83.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTAC---AGTCCGA 44 + |. |||| |||||||.|.| .|||| +FC12044_91407 1 -----TT--GCGA-------------CAGAGTTTTGCTCTTGTCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 21/39 (53.8%) +# Score: 78.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTT----CAGAGTTCTAC---AGTCCGACATG 32 + || |||||||.|.| .|||| +FC12044_91407 1 --------TTGCGACAGAGTTTTGCTCTTGTCC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 31.0 +# +# +#======================================= + +Illumina_Smal 1 ---CAAGCAGA----------AGACGGCATACGA 21 + |.| |||| .|.| | +FC12044_91407 1 TTGCGA-CAGAGTTTTGCTCTTGTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 15/33 (45.5%) +# Similarity: 15/33 (45.5%) +# Gaps: 15/33 (45.5%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Smal 1 GTT----CAGAGTTCTAC---AGTCCGACGATC 26 + || |||||||.|.| .|||| +FC12044_91407 1 -TTGCGACAGAGTTTTGCTCTTGTCC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 15/30 (50.0%) +# Similarity: 15/30 (50.0%) +# Gaps: 13/30 (43.3%) +# Score: 63.5 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGC--C---GTCTT-CTGCTTGT-- 22 + .||| | ||.|| || ||||| +FC12044_91407 1 ----TTGCGACAGAGTTTTGCT-CTTGTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 31.0 +# +# +#======================================= + +Illumina_Smal 1 ---CAAGCAGA----------AGACGGCATACGA 21 + |.| |||| .|.| | +FC12044_91407 1 TTGCGA-CAGAGTTTTGCTCTTGTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 83.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTAC---AGTCCGA 44 + |. |||| |||||||.|.| .|||| +FC12044_91407 1 -----TT--GCGA-------------CAGAGTTTTGCTCTTGTCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 17/39 (43.6%) +# Similarity: 17/39 (43.6%) +# Gaps: 21/39 (53.8%) +# Score: 86.5 +# +# +#======================================= + +Illumina_Smal 1 ---CGACAG-GTT---CAGAGTTCTACAGTCCGACGATC 32 + |||||| ||| | ||| .|||| +FC12044_91407 1 TTGCGACAGAGTTTTGC-----TCT--TGTCC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 15/40 (37.5%) +# Similarity: 15/40 (37.5%) +# Gaps: 22/40 (55.0%) +# Score: 55.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAG----AGCTC---GTATGCCGTCTTCTGCTTG 33 + || .| ||||| | |.||||.| +FC12044_91407 1 --TC---TGCTCCAGCTCCAAG-ACGCCGCC--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTT-TCC--CTACACGACGCTCTTCCGATCT 33 + |||. ||| ||.||.||||| | .|| +FC12044_91407 1 ----TCTGCTCCAGCTCCAAGACGC-C-GCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 60 +# Identity: 19/60 (31.7%) +# Similarity: 19/60 (31.7%) +# Gaps: 37/60 (61.7%) +# Score: 99.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCC--CTACACGACGCT 48 + |||.| ||| ||.||.||||| +FC12044_91407 1 ----------------------TCTGC------TCCAGCTCCAAGACGC- 21 + +Illumina_Geno 49 CTTCCGATCT 58 + | .|| +FC12044_91407 22 C-GCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 15/44 (34.1%) +# Similarity: 15/44 (34.1%) +# Gaps: 29/44 (65.9%) +# Score: 61.5 +# +# +#======================================= + +Illumina_Geno 1 ------CAAGCAG----AAGACGGCATACGAGCTCTTCCGATCT 34 + | ||| ||||| || || || +FC12044_91407 1 TCTGCTC---CAGCTCCAAGAC-GC---CG-------CC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTT-TCC--CTACACGACGCTCTTCCGATCT 33 + |||. ||| ||.||.||||| | .|| +FC12044_91407 1 ----TCTGCTCCAGCTCCAAGACGC-C-GCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 23/40 (57.5%) +# Score: 57.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG-------- 32 + || .|| |.||||. ||.|| +FC12044_91407 1 --TC-----TGC--TCCAGCT------CCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTT-TCC--CTACACGACGCTCTTCCGATCT 33 + |||. ||| ||.||.||||| | .|| +FC12044_91407 1 ----TCTGCTCCAGCTCCAAGACGC-C-GCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 60 +# Identity: 19/60 (31.7%) +# Similarity: 19/60 (31.7%) +# Gaps: 37/60 (61.7%) +# Score: 99.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCC--CTACACGACGCT 48 + |||.| ||| ||.||.||||| +FC12044_91407 1 ----------------------TCTGC------TCCAGCTCCAAGACGC- 21 + +Illumina_Pair 49 CTTCCGATCT 58 + | .|| +FC12044_91407 22 C-GCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 18/66 (27.3%) +# Similarity: 18/66 (27.3%) +# Gaps: 46/66 (69.7%) +# Score: 92.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCT----GA- 45 + ||| || |||.||| || +FC12044_91407 1 ---------------------------TCT--GC--TCCAGCTCCAAGAC 19 + +Illumina_Pair 46 ACCGCTCTTCCGATCT 61 + .|||| | +FC12044_91407 20 GCCGC-C--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTT-TCC--CTACACGACGCTCTTCCGATCT 33 + |||. ||| ||.||.||||| | .|| +FC12044_91407 1 ----TCTGCTCCAGCTCCAAGACGC-C-GCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 18/42 (42.9%) +# Similarity: 18/42 (42.9%) +# Gaps: 22/42 (52.4%) +# Score: 92.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCT----GA-ACCGCTCTTCCGATCT 37 + ||| || |||.||| || .|||| | +FC12044_91407 1 ---TCT--GC--TCCAGCTCCAAGACGCCGC-C--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 13/35 (37.1%) +# Similarity: 13/35 (37.1%) +# Gaps: 19/35 (54.3%) +# Score: 41.0 +# +# +#======================================= + +Illumina_DpnI 1 GATC-G-TCGGACTGTAGAACTCTGA-AC------ 26 + || | || |.| |||..| || +FC12044_91407 1 --TCTGCTC---CAG-----CTCCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 14/35 (40.0%) +# Similarity: 14/35 (40.0%) +# Gaps: 19/35 (54.3%) +# Score: 72.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACAG-TCC--GAC------ 26 + ||.| ||.||| ||| ||| +FC12044_91407 1 ------TCTG----CTCCAGCTCCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 13/33 (39.4%) +# Similarity: 13/33 (39.4%) +# Gaps: 20/33 (60.6%) +# Score: 54.0 +# +# +#======================================= + +Illumina_DpnI 1 ------CAAGCAG----AAGACG--GCATACGA 21 + | ||| |||||| || | +FC12044_91407 1 TCTGCTC---CAGCTCCAAGACGCCGC---C-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 51.0 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGCCGTCTTCTGCT---TG-------- 21 + || ||| || |.||| .| +FC12044_91407 1 TC---TGC--TC--CAGCTCCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 13/33 (39.4%) +# Similarity: 13/33 (39.4%) +# Gaps: 20/33 (60.6%) +# Score: 54.0 +# +# +#======================================= + +Illumina_DpnI 1 ------CAAGCAG----AAGACG--GCATACGA 21 + | ||| |||||| || | +FC12044_91407 1 TCTGCTC---CAGCTCCAAGACGCCGC---C-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 12/54 (22.2%) +# Similarity: 12/54 (22.2%) +# Gaps: 39/54 (72.2%) +# Score: 64.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA----- 44 + ||.| ||.||| |||.| +FC12044_91407 1 -------------------------TCTG----CTCCAGCTCCAAGACGC 21 + +Illumina_DpnI 45 ---- 44 + +FC12044_91407 22 CGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 21/39 (53.8%) +# Score: 77.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACAG-TCC--GACGATC---- 32 + ||.| ||.||| ||| |||| | +FC12044_91407 1 --------TCTG----CTCCAGCTCCAAGACG--CCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 16/31 (51.6%) +# Score: 39.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTG-T--AGAACTCTGA-AC------ 21 + .||| | || |||..| || +FC12044_91407 1 ----TCTGCTCCAG--CTCCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 15/37 (40.5%) +# Similarity: 15/37 (40.5%) +# Gaps: 20/37 (54.1%) +# Score: 67.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAG-TCC--GACATG----- 29 + ||.| ||.||| ||| ||| | +FC12044_91407 1 ------TCTG----CTCCAGCTCCAAGAC--GCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 13/35 (37.1%) +# Similarity: 13/35 (37.1%) +# Gaps: 22/35 (62.9%) +# Score: 54.0 +# +# +#======================================= + +Illumina_NlaI 1 ------CAAGCAG----AAGACG--GCATACGANN 23 + | ||| |||||| || | +FC12044_91407 1 TCTGCTC---CAGCTCCAAGACGCCGC---C---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 51.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGCCGTCTTCTGCT---TG-------- 21 + || ||| || |.||| .| +FC12044_91407 1 TC---TGC--TC--CAGCTCCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 13/33 (39.4%) +# Similarity: 13/33 (39.4%) +# Gaps: 20/33 (60.6%) +# Score: 54.0 +# +# +#======================================= + +Illumina_NlaI 1 ------CAAGCAG----AAGACG--GCATACGA 21 + | ||| |||||| || | +FC12044_91407 1 TCTGCTC---CAGCTCCAAGACGCCGC---C-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 12/54 (22.2%) +# Similarity: 12/54 (22.2%) +# Gaps: 39/54 (72.2%) +# Score: 64.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA----- 44 + ||.| ||.||| |||.| +FC12044_91407 1 -------------------------TCTG----CTCCAGCTCCAAGACGC 21 + +Illumina_NlaI 45 ---- 44 + +FC12044_91407 22 CGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 15/40 (37.5%) +# Similarity: 15/40 (37.5%) +# Gaps: 23/40 (57.5%) +# Score: 67.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGAGTTCTACAG-TCC--GACATG----- 32 + ||.| ||.||| ||| ||| | +FC12044_91407 1 ---------TCTG----CTCCAGCTCCAAGAC--GCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 13/33 (39.4%) +# Similarity: 13/33 (39.4%) +# Gaps: 20/33 (60.6%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Smal 1 ------CAAGCAG----AAGACG--GCATACGA 21 + | ||| |||||| || | +FC12044_91407 1 TCTGCTC---CAGCTCCAAGACGCCGC---C-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 16/33 (48.5%) +# Similarity: 16/33 (48.5%) +# Gaps: 15/33 (45.5%) +# Score: 77.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAG-TCC--GACGATC---- 26 + ||.| ||.||| ||| |||| | +FC12044_91407 1 --TCTG----CTCCAGCTCCAAGACG--CCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 17/32 (53.1%) +# Score: 50.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGCCGTCTTCTGCT---TGT------- 22 + || ||| || |.||| .|. +FC12044_91407 1 TC---TGC--TC--CAGCTCCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 13/33 (39.4%) +# Similarity: 13/33 (39.4%) +# Gaps: 20/33 (60.6%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Smal 1 ------CAAGCAG----AAGACG--GCATACGA 21 + | ||| |||||| || | +FC12044_91407 1 TCTGCTC---CAGCTCCAAGACGCCGC---C-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 12/54 (22.2%) +# Similarity: 12/54 (22.2%) +# Gaps: 39/54 (72.2%) +# Score: 64.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA----- 44 + ||.| ||.||| |||.| +FC12044_91407 1 -------------------------TCTG----CTCCAGCTCCAAGACGC 21 + +Illumina_Smal 45 ---- 44 + +FC12044_91407 22 CGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 21/39 (53.8%) +# Score: 77.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACAG-TCC--GACGATC---- 32 + ||.| ||.||| ||| |||| | +FC12044_91407 1 --------TCTG----CTCCAGCTCCAAGACG--CCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 12/37 (32.4%) +# Similarity: 12/37 (32.4%) +# Gaps: 16/37 (43.2%) +# Score: 40.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAA----GAGCTCGTATGCCGTCTTCTGCTTG 33 + ..||||. |.||..|.|.|.|| +FC12044_91407 1 -TACGGAGCCGCGGGCGGGAAAGGCG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 29.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGACGCTCTTC-CGATCT--------- 33 + |||. || || | ||..|. +FC12044_91407 1 ------------TACG-GA-GC----CGCGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 17/61 (27.9%) +# Similarity: 17/61 (27.9%) +# Gaps: 39/61 (63.9%) +# Score: 69.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCAC---CGAGATCTACACTCTTTCCCTACACGACGC 47 + |||||.| ||.| ||.|| |.|.|| +FC12044_91407 1 -----TACGGAG-CCGCGGGCGGGA------------------AAGGCG- 25 + +Illumina_Geno 48 TCTTCCGATCT 58 + +FC12044_91407 26 ----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 9/46 (19.6%) +# Similarity: 9/46 (19.6%) +# Gaps: 33/46 (71.7%) +# Score: 47.5 +# +# +#======================================= + +Illumina_Geno 1 ----------CAAGCAG--AAGACGGCATACGAGCTCTTCCGATCT 34 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG--------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 29.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGACGCTCTTC-CGATCT--------- 33 + |||. || || | ||..|. +FC12044_91407 1 ------------TACG-GA-GC----CGCGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 17/32 (53.1%) +# Similarity: 17/32 (53.1%) +# Gaps: 7/32 (21.9%) +# Score: 75.5 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG 32 + ..||||...|||| ||.||||.|.|| +FC12044_91407 1 -TACGGAGCCGCGG----GCGGGAAAGGCG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 29.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGACGCTCTTC-CGATCT--------- 33 + |||. || || | ||..|. +FC12044_91407 1 ------------TACG-GA-GC----CGCGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 17/61 (27.9%) +# Similarity: 17/61 (27.9%) +# Gaps: 39/61 (63.9%) +# Score: 69.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCAC---CGAGATCTACACTCTTTCCCTACACGACGC 47 + |||||.| ||.| ||.|| |.|.|| +FC12044_91407 1 -----TACGGAG-CCGCGGGCGGGA------------------AAGGCG- 25 + +Illumina_Pair 48 TCTTCCGATCT 58 + +FC12044_91407 26 ----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 17/64 (26.6%) +# Similarity: 17/64 (26.6%) +# Gaps: 42/64 (65.6%) +# Score: 56.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAAC--- 47 + |||| ||.| |.||| ||.|.|. +FC12044_91407 1 ----------------TACG-GAGC---CGCGG-------GCGGGAAAGG 23 + +Illumina_Pair 48 CGCTCTTCCGATCT 61 + || +FC12044_91407 24 CG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 29.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGACGCTCTTC-CGATCT--------- 33 + |||. || || | ||..|. +FC12044_91407 1 ------------TACG-GA-GC----CGCGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 11/48 (22.9%) +# Similarity: 11/48 (22.9%) +# Gaps: 34/48 (70.8%) +# Score: 45.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAACCGCTCTT---C--------CGATCT 37 + |.|.||.|||| | || +FC12044_91407 1 ---------------TACGGAGCCGC----GGGCGGGAAAGGCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 13/35 (37.1%) +# Similarity: 13/35 (37.1%) +# Gaps: 19/35 (54.3%) +# Score: 35.0 +# +# +#======================================= + +Illumina_DpnI 1 ----GAT-CGTCGGACTGTAGAACTCTGAA---C- 26 + ||. || |||.|.| ||| | +FC12044_91407 1 TACGGAGCCG-CGGGCGG---------GAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 9/40 (22.5%) +# Similarity: 9/40 (22.5%) +# Gaps: 29/40 (72.5%) +# Score: 44.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACAGTCCGAC-------------- 26 + |.|.||| ||| | +FC12044_91407 1 -----TACGGAG---------CCG-CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 47.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------CAAGCAG--AAGACGGCATACGA 21 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 31.0 +# +# +#======================================= + +Illumina_DpnI 1 -TCGTATGCCGTCTTC-TGCTTG--------- 21 + .||.| |||| | .|| | +FC12044_91407 1 TACGGA-GCCG----CGGGC--GGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 47.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------CAAGCAG--AAGACGGCATACGA 21 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 19/44 (43.2%) +# Similarity: 19/44 (43.2%) +# Gaps: 19/44 (43.2%) +# Score: 71.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA 44 + |||||.| ||.|.|.|.|| |.|| | || +FC12044_91407 1 -----TACGGAG-CCGCGGGCGGG---AAAG-------G--CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 11/42 (26.2%) +# Similarity: 11/42 (26.2%) +# Gaps: 27/42 (64.3%) +# Score: 52.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACAGTCCGACGATC---------- 32 + |.|.||| ||| ||..| +FC12044_91407 1 -------TACGGAG---------CCG-CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 30.0 +# +# +#======================================= + +Illumina_NlaI 1 -TCGGA-CTGTAGAACTCTGAAC---------- 21 + .|||| | |.| |..| +FC12044_91407 1 TACGGAGC-------CGC-GGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 10/40 (25.0%) +# Similarity: 10/40 (25.0%) +# Gaps: 26/40 (65.0%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAGTCCGACATG----------- 29 + |.|.||| ||| |..| +FC12044_91407 1 -----TACGGAG---------CCG-CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 22/35 (62.9%) +# Score: 47.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------CAAGCAG--AAGACGGCATACGANN 23 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 31.0 +# +# +#======================================= + +Illumina_NlaI 1 -TCGTATGCCGTCTTC-TGCTTG--------- 21 + .||.| |||| | .|| | +FC12044_91407 1 TACGGA-GCCG----CGGGC--GGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 47.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------CAAGCAG--AAGACGGCATACGA 21 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 19/44 (43.2%) +# Similarity: 19/44 (43.2%) +# Gaps: 19/44 (43.2%) +# Score: 71.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA 44 + |||||.| ||.|.|.|.|| |.|| | || +FC12044_91407 1 -----TACGGAG-CCGCGGGCGGG---AAAG-------G--CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 10/43 (23.3%) +# Similarity: 10/43 (23.3%) +# Gaps: 29/43 (67.4%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGAGTTCTACAGTCCGACATG----------- 32 + |.|.||| ||| |..| +FC12044_91407 1 --------TACGGAG---------CCG-CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 47.5 +# +# +#======================================= + +Illumina_Smal 1 ----------CAAGCAG--AAGACGGCATACGA 21 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 11/36 (30.6%) +# Similarity: 11/36 (30.6%) +# Gaps: 21/36 (58.3%) +# Score: 52.0 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAGTCCGACGATC---------- 26 + |.|.||| ||| ||..| +FC12044_91407 1 -TACGGAG---------CCG-CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 11/30 (36.7%) +# Similarity: 11/30 (36.7%) +# Gaps: 13/30 (43.3%) +# Score: 28.5 +# +# +#======================================= + +Illumina_Smal 1 -TCGTATGCCGTCTTC-TGCTTGT------ 22 + .||.| |||| | .||..|. +FC12044_91407 1 TACGGA-GCCG----CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 47.5 +# +# +#======================================= + +Illumina_Smal 1 ----------CAAGCAG--AAGACGGCATACGA 21 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 19/44 (43.2%) +# Similarity: 19/44 (43.2%) +# Gaps: 19/44 (43.2%) +# Score: 71.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA 44 + |||||.| ||.|.|.|.|| |.|| | || +FC12044_91407 1 -----TACGGAG-CCGCGGGCGGG---AAAG-------G--CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 11/42 (26.2%) +# Similarity: 11/42 (26.2%) +# Gaps: 27/42 (64.3%) +# Score: 52.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACAGTCCGACGATC---------- 32 + |.|.||| ||| ||..| +FC12044_91407 1 -------TACGGAG---------CCG-CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 28/43 (65.1%) +# Score: 50.5 +# +# +#======================================= + +Illumina_Geno 1 --------GATCGGAAGAGCTCG--TATGCCGTCTTCTGCTTG 33 + |.|| ||| || ||| || | +FC12044_91407 1 CCTCCCAGGTTC--AAG----CGATTAT-CC-T---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 18/39 (46.2%) +# Similarity: 18/39 (46.2%) +# Gaps: 20/39 (51.3%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCC---TACA--CGACGCTCTTCCGAT-CT 33 + | | |||| |.|| ||| || || || +FC12044_91407 1 ---C-C--TCCCAGGTTCAAGCGA-----TT---ATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 18/64 (28.1%) +# Similarity: 18/64 (28.1%) +# Gaps: 45/64 (70.3%) +# Score: 73.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCC---TACA--CGAC 45 + || |||| |.|| ||| +FC12044_91407 1 ----------------CC---------------TCCCAGGTTCAAGCGA- 18 + +Illumina_Geno 46 GCTCTTCCGAT-CT 58 + || || || +FC12044_91407 19 ----TT---ATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 13/46 (28.3%) +# Similarity: 13/46 (28.3%) +# Gaps: 33/46 (71.7%) +# Score: 61.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACGAGCTC-----TTC---CGAT----CT 34 + | ||| ||| |||| || +FC12044_91407 1 ------------------C---CTCCCAGGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 18/39 (46.2%) +# Similarity: 18/39 (46.2%) +# Gaps: 20/39 (51.3%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCC---TACA--CGACGCTCTTCCGAT-CT 33 + | | |||| |.|| ||| || || || +FC12044_91407 1 ---C-C--TCCCAGGTTCAAGCGA-----TT---ATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 23/40 (57.5%) +# Score: 55.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGC------GGTTC-AGCAGGAATG-CCGAG 32 + | ||||| ||| ||.|. ||. +FC12044_91407 1 -----------CCTCCCAGGTTCAAGC--GATTATCCT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 18/39 (46.2%) +# Similarity: 18/39 (46.2%) +# Gaps: 20/39 (51.3%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCC---TACA--CGACGCTCTTCCGAT-CT 33 + | | |||| |.|| ||| || || || +FC12044_91407 1 ---C-C--TCCCAGGTTCAAGCGA-----TT---ATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 18/64 (28.1%) +# Similarity: 18/64 (28.1%) +# Gaps: 45/64 (70.3%) +# Score: 73.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCC---TACA--CGAC 45 + || |||| |.|| ||| +FC12044_91407 1 ----------------CC---------------TCCCAGGTTCAAGCGA- 18 + +Illumina_Pair 46 GCTCTTCCGAT-CT 58 + || || || +FC12044_91407 19 ----TT---ATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 71 +# Identity: 15/71 (21.1%) +# Similarity: 15/71 (21.1%) +# Gaps: 56/71 (78.9%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGC 50 + ||| || +FC12044_91407 1 -------------------------------------CCT------CC-- 5 + +Illumina_Pair 51 TC---TTC---CGAT----CT 61 + | ||| |||| || +FC12044_91407 6 -CAGGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 18/39 (46.2%) +# Similarity: 18/39 (46.2%) +# Gaps: 20/39 (51.3%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCC---TACA--CGACGCTCTTCCGAT-CT 33 + | | |||| |.|| ||| || || || +FC12044_91407 1 ---C-C--TCCCAGGTTCAAGCGA-----TT---ATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 15/47 (31.9%) +# Similarity: 15/47 (31.9%) +# Gaps: 32/47 (68.1%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAACCGCTC---TTC---CGAT----CT 37 + ||| || | ||| |||| || +FC12044_91407 1 -------------CCT------CC---CAGGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 7/42 (16.7%) +# Similarity: 7/42 (16.7%) +# Gaps: 33/42 (78.6%) +# Score: 32.0 +# +# +#======================================= + +Illumina_DpnI 1 ----------------GATCGTCGGACTGTAGAACTCTGAAC 26 + |||..|| || +FC12044_91407 1 CCTCCCAGGTTCAAGCGATTATC---CT-------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 16/31 (51.6%) +# Similarity: 16/31 (51.6%) +# Gaps: 11/31 (35.5%) +# Score: 67.0 +# +# +#======================================= + +Illumina_DpnI 1 ----ACAGGTTC-AGAGTTCTACAGTCCGAC 26 + .||||||| ||.|.| || |||. +FC12044_91407 1 CCTCCCAGGTTCAAGCGAT-TA---TCCT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_DpnI 1 ----CAAGCAGAAGACGG--CATACGA------- 21 + | || || ||..||| +FC12044_91407 1 CCTCC---CA------GGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 11/33 (33.3%) +# Similarity: 11/33 (33.3%) +# Gaps: 20/33 (60.6%) +# Score: 45.5 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGCCGTC-----TTC-TGC--TTG---- 21 + || || ||| .|| ||. +FC12044_91407 1 -------CC-TCCCAGGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_DpnI 1 ----CAAGCAGAAGACGG--CATACGA------- 21 + | || || ||..||| +FC12044_91407 1 CCTCC---CA------GGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 101.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTC-AGAGTTCTACAGTCCGA 44 + ||.|| ||||||| ||.|.| || |||. +FC12044_91407 1 -------------CCTCC--CAGGTTCAAGCGAT-TA---TCCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 17/38 (44.7%) +# Similarity: 17/38 (44.7%) +# Gaps: 19/38 (50.0%) +# Score: 76.0 +# +# +#======================================= + +Illumina_DpnI 1 ----CGACAGGTTCAGAGTTCTACAGTCCGACGATC-- 32 + | |||||||| || |||..||| +FC12044_91407 1 CCTCC--CAGGTTCA-AG----------CGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 8/31 (25.8%) +# Similarity: 8/31 (25.8%) +# Gaps: 16/31 (51.6%) +# Score: 31.0 +# +# +#======================================= + +Illumina_NlaI 1 ----------TCGGACTGTAGAACTCTGAAC 21 + ||...| |...|.| || +FC12044_91407 1 CCTCCCAGGTTCAAGC-GATTATC-CT---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 17/34 (50.0%) +# Similarity: 17/34 (50.0%) +# Gaps: 14/34 (41.2%) +# Score: 68.5 +# +# +#======================================= + +Illumina_NlaI 1 ----ACAGGTTC-AGAGTTCTACAGTCCGACATG 29 + .||||||| ||.|.| || ||| | +FC12044_91407 1 CCTCCCAGGTTCAAGCGAT-TA---TCC----T- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 7/34 (20.6%) +# Similarity: 7/34 (20.6%) +# Gaps: 20/34 (58.8%) +# Score: 37.5 +# +# +#======================================= + +Illumina_NlaI 1 -----------CAAGCAGAAGACGGCATACGANN 23 + |||||......| |. +FC12044_91407 1 CCTCCCAGGTTCAAGCGATTATC--CT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 11/33 (33.3%) +# Similarity: 11/33 (33.3%) +# Gaps: 20/33 (60.6%) +# Score: 45.5 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGCCGTC-----TTC-TGC--TTG---- 21 + || || ||| .|| ||. +FC12044_91407 1 -------CC-TCCCAGGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_NlaI 1 ----CAAGCAGAAGACGG--CATACGA------- 21 + | || || ||..||| +FC12044_91407 1 CCTCC---CA------GGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 101.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTC-AGAGTTCTACAGTCCGA 44 + ||.|| ||||||| ||.|.| || |||. +FC12044_91407 1 -------------CCTCC--CAGGTTCAAGCGAT-TA---TCCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 19/36 (52.8%) +# Similarity: 19/36 (52.8%) +# Gaps: 15/36 (41.7%) +# Score: 84.0 +# +# +#======================================= + +Illumina_NlaI 1 ---CCGACAGGTTC-AGAGTTCTACAGTCCGACATG 32 + || ||||||| ||.|.| || ||| | +FC12044_91407 1 CCTCC--CAGGTTCAAGCGAT-TA---TCC----T- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_Smal 1 ----CAAGCAGAAGACGG--CATACGA------- 21 + | || || ||..||| +FC12044_91407 1 CCTCC---CA------GGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 54.5 +# +# +#======================================= + +Illumina_Smal 1 --------GTTCAGAGTTCTACAGTCCGACGATC-- 26 + ||||| || |||..||| +FC12044_91407 1 CCTCCCAGGTTCA-AG----------CGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 12/33 (36.4%) +# Similarity: 12/33 (36.4%) +# Gaps: 19/33 (57.6%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGCCGTC-----TTC-TGC--TTGT--- 22 + || || ||| .|| ||.| +FC12044_91407 1 -------CC-TCCCAGGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_Smal 1 ----CAAGCAGAAGACGG--CATACGA------- 21 + | || || ||..||| +FC12044_91407 1 CCTCC---CA------GGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 101.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTC-AGAGTTCTACAGTCCGA 44 + ||.|| ||||||| ||.|.| || |||. +FC12044_91407 1 -------------CCTCC--CAGGTTCAAGCGAT-TA---TCCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 17/38 (44.7%) +# Similarity: 17/38 (44.7%) +# Gaps: 19/38 (50.0%) +# Score: 76.0 +# +# +#======================================= + +Illumina_Smal 1 ----CGACAGGTTCAGAGTTCTACAGTCCGACGATC-- 32 + | |||||||| || |||..||| +FC12044_91407 1 CCTCC--CAGGTTCA-AG----------CGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 12/43 (27.9%) +# Similarity: 12/43 (27.9%) +# Gaps: 28/43 (65.1%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGT-ATGCCGTCTTCTGCTTG--------- 33 + || |||.||.| |.|| | +FC12044_91407 1 --------------GTCATGGCGGC--CCGC--GCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Geno 1 -ACACTCTTT-----CCCTACACG----ACGCTCTTCCGATCT 33 + .|| | ||| .|.|| .|| +FC12044_91407 1 GTCA-----TGGCGGCCC-GCGCGGGGAGCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 17/62 (27.4%) +# Similarity: 17/62 (27.4%) +# Gaps: 41/62 (66.1%) +# Score: 58.0 +# +# +#======================================= + +Illumina_Geno 1 --AATGATACGGCGACCAC-CGAGATCTACACTCTTTCCCTACACGA-CG 46 + .||| .|||| ||.| ||.| || || +FC12044_91407 1 GTCATG--GCGGC--CCGCGCGGG---------------------GAGCG 25 + +Illumina_Geno 47 CTCTTCCGATCT 58 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 12/44 (27.3%) +# Similarity: 12/44 (27.3%) +# Gaps: 29/44 (65.9%) +# Score: 46.0 +# +# +#======================================= + +Illumina_Geno 1 --CAAGCAGAAGACGGCATAC--------GAGCTCTTCCGATCT 34 + | |.|.|||| | ||||. +FC12044_91407 1 GTC------ATGGCGGC---CCGCGCGGGGAGCG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Geno 1 -ACACTCTTT-----CCCTACACG----ACGCTCTTCCGATCT 33 + .|| | ||| .|.|| .|| +FC12044_91407 1 GTCA-----TGGCGGCCC-GCGCGGGGAGCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 17/35 (48.6%) +# Similarity: 17/35 (48.6%) +# Gaps: 13/35 (37.1%) +# Score: 48.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTC---AGCAGGAATGCCGAG 32 + .|| |.| |||| | .||.||.| | || +FC12044_91407 1 -GTC--ATG-GCGG--CCCGCGCGGGGA-G-CG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Pair 1 -ACACTCTTT-----CCCTACACG----ACGCTCTTCCGATCT 33 + .|| | ||| .|.|| .|| +FC12044_91407 1 GTCA-----TGGCGGCCC-GCGCGGGGAGCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 17/62 (27.4%) +# Similarity: 17/62 (27.4%) +# Gaps: 41/62 (66.1%) +# Score: 58.0 +# +# +#======================================= + +Illumina_Pair 1 --AATGATACGGCGACCAC-CGAGATCTACACTCTTTCCCTACACGA-CG 46 + .||| .|||| ||.| ||.| || || +FC12044_91407 1 GTCATG--GCGGC--CCGCGCGGG---------------------GAGCG 25 + +Illumina_Pair 47 CTCTTCCGATCT 58 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 70 +# Identity: 14/70 (20.0%) +# Similarity: 14/70 (20.0%) +# Gaps: 54/70 (77.1%) +# Score: 60.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCT---CGGCATTCCTGCTGAAC 47 + |||. |||| | +FC12044_91407 1 --------------------------GTCATGGCGGC------------C 12 + +Illumina_Pair 48 CGCTCTTC------CGATCT 61 + ||| .| || +FC12044_91407 13 CGC---GCGGGGAGCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Pair 1 -ACACTCTTT-----CCCTACACG----ACGCTCTTCCGATCT 33 + .|| | ||| .|.|| .|| +FC12044_91407 1 GTCA-----TGGCGGCCC-GCGCGGGGAGCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 14/46 (30.4%) +# Similarity: 14/46 (30.4%) +# Gaps: 30/46 (65.2%) +# Score: 60.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCT---CGGCATTCCTGCTGAACCGCTCTTC------CGATCT 37 + |||. |||| |||| .| || +FC12044_91407 1 --GTCATGGCGGC------------CCGC---GCGGGGAGCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 12/33 (36.4%) +# Similarity: 12/33 (36.4%) +# Gaps: 15/33 (45.5%) +# Score: 39.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGT--CGGACTGTAGAACTC----TGAAC- 26 + .||.| |||.| |.| .||.| +FC12044_91407 1 -GTCATGGCGGCC-------CGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 11/36 (30.6%) +# Similarity: 11/36 (30.6%) +# Gaps: 21/36 (58.3%) +# Score: 37.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACAGTCCGAC---------- 26 + | |||..| |.|.||| | +FC12044_91407 1 ---G--TCATGG-----CGGCCCG-CGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 36.0 +# +# +#======================================= + +Illumina_DpnI 1 --CAAGCAGAAGACGGCATAC--------GA--- 21 + | |.|.|||| | || +FC12044_91407 1 GTC------ATGGCGGC---CCGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 16/31 (51.6%) +# Score: 39.5 +# +# +#======================================= + +Illumina_DpnI 1 -TCGTATGCCGTCTTCTGCTTG--------- 21 + || |||.||.| |.|| | +FC12044_91407 1 GTC--ATGGCGGC--CCGC--GCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 36.0 +# +# +#======================================= + +Illumina_DpnI 1 --CAAGCAGAAGACGGCATAC--------GA--- 21 + | |.|.|||| | || +FC12044_91407 1 GTC------ATGGCGGC---CCGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 68.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCAC-CGACAGGTTCAGAGTTCTACAGTCCGA 44 + .|.|| ||||.|| | || |.|| ||| || +FC12044_91407 1 -GTCAT--GGCGGCC-CGCG-CGGG----GAG----------CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 25/41 (61.0%) +# Score: 45.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACA-GGTTCAGAGTTCTACAGTCCGACGATC-------- 32 + |.|| || |.|.||| || | +FC12044_91407 1 -GTCATGG------------CGGCCCG-CG--CGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 26.0 +# +# +#======================================= + +Illumina_NlaI 1 ----T--CGGACTGTAGAACTC----TGAAC- 21 + | |||.| |.| .||.| +FC12044_91407 1 GTCATGGCGGCC-------CGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 5/48 (10.4%) +# Similarity: 5/48 (10.4%) +# Gaps: 42/48 (87.5%) +# Score: 34.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAGTCCGACATG------------------- 29 + |.|||| +FC12044_91407 1 -----------------------GTCATGGCGGCCCGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 20/34 (58.8%) +# Score: 35.5 +# +# +#======================================= + +Illumina_NlaI 1 --CAAGCAGAAGACGGC---------ATACGANN 23 + | |.|.|||| ...|| +FC12044_91407 1 GTC------ATGGCGGCCCGCGCGGGGAGCG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 16/31 (51.6%) +# Score: 39.5 +# +# +#======================================= + +Illumina_NlaI 1 -TCGTATGCCGTCTTCTGCTTG--------- 21 + || |||.||.| |.|| | +FC12044_91407 1 GTC--ATGGCGGC--CCGC--GCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 36.0 +# +# +#======================================= + +Illumina_NlaI 1 --CAAGCAGAAGACGGCATAC--------GA--- 21 + | |.|.|||| | || +FC12044_91407 1 GTC------ATGGCGGC---CCGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 68.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCAC-CGACAGGTTCAGAGTTCTACAGTCCGA 44 + .|.|| ||||.|| | || |.|| ||| || +FC12044_91407 1 -GTCAT--GGCGGCC-CGCG-CGGG----GAG----------CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 11/44 (25.0%) +# Similarity: 11/44 (25.0%) +# Gaps: 31/44 (70.5%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 -----------CCG-ACAGGTTCAGAGTTCTACAGTCCGACATG 32 + ||| .|.|| ||| || +FC12044_91407 1 GTCATGGCGGCCCGCGCGGG----GAG----------CG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Smal 1 --CAAGCAGAAGACGGCATAC--------GA--- 21 + | |.|.|||| | || +FC12044_91407 1 GTC------ATGGCGGC---CCGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 13/34 (38.2%) +# Similarity: 13/34 (38.2%) +# Gaps: 17/34 (50.0%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAGTCCGACGATC-------- 26 + | |||..| |.|.||| || | +FC12044_91407 1 G-TCATGG-----CGGCCCG-CG--CGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 12/34 (35.3%) +# Similarity: 12/34 (35.3%) +# Gaps: 21/34 (61.8%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Smal 1 -TCGTATG-----CCGTCTTC------TGCTTGT 22 + || ||| ||| | .|| | +FC12044_91407 1 GTC--ATGGCGGCCCG----CGCGGGGAGC--G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Smal 1 --CAAGCAGAAGACGGCATAC--------GA--- 21 + | |.|.|||| | || +FC12044_91407 1 GTC------ATGGCGGC---CCGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 68.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCAC-CGACAGGTTCAGAGTTCTACAGTCCGA 44 + .|.|| ||||.|| | || |.|| ||| || +FC12044_91407 1 -GTCAT--GGCGGCC-CGCG-CGGG----GAG----------CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 25/41 (61.0%) +# Score: 45.0 +# +# +#======================================= + +Illumina_Smal 1 CGACA-GGTTCAGAGTTCTACAGTCCGACGATC-------- 32 + |.|| || |.|.||| || | +FC12044_91407 1 -GTCATGG------------CGGCCCG-CG--CGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 12/44 (27.3%) +# Similarity: 12/44 (27.3%) +# Gaps: 30/44 (68.2%) +# Score: 57.5 +# +# +#======================================= + +Illumina_Geno 1 -------GATC----GGAAGAGCTCGTATGCCGTCTTCTGCTTG 33 + |.|| .|||||| ||| +FC12044_91407 1 ACAGTGGGTTCTTAAAGAAGAG-TCG------------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 32/45 (71.1%) +# Score: 36.5 +# +# +#======================================= + +Illumina_Geno 1 ACAC-------TCTTTCCCTACACGA-----CGCTCTTCCGATCT 33 + || ||||. |.|| || +FC12044_91407 1 --ACAGTGGGTTCTTA------AAGAAGAGTCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 14/64 (21.9%) +# Similarity: 14/64 (21.9%) +# Gaps: 45/64 (70.3%) +# Score: 37.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGA-GATCTACACTCTTTCCCTACACGA----- 44 + ||.|. |.| ||||. |.|| +FC12044_91407 1 ---------------ACAGTGGGT------TCTTA------AAGAAGAGT 23 + +Illumina_Geno 45 CGCTCTTCCGATCT 58 + || +FC12044_91407 24 CG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 11/48 (22.9%) +# Similarity: 11/48 (22.9%) +# Gaps: 37/48 (77.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Geno 1 ----------C--AAGCAGAAGA--CGGCATACGAGCTCTTCCGATCT 34 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG--------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 32/45 (71.1%) +# Score: 36.5 +# +# +#======================================= + +Illumina_Geno 1 ACAC-------TCTTTCCCTACACGA-----CGCTCTTCCGATCT 33 + || ||||. |.|| || +FC12044_91407 1 --ACAGTGGGTTCTTA------AAGAAGAGTCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 17/36 (47.2%) +# Similarity: 17/36 (47.2%) +# Gaps: 15/36 (41.7%) +# Score: 58.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTC----AGCAGGAATGCCGAG 32 + .| ||.| ||||| || |.||.| || +FC12044_91407 1 --AC---AGTG-GGTTCTTAAAG-AAGAGT--CG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 32/45 (71.1%) +# Score: 36.5 +# +# +#======================================= + +Illumina_Pair 1 ACAC-------TCTTTCCCTACACGA-----CGCTCTTCCGATCT 33 + || ||||. |.|| || +FC12044_91407 1 --ACAGTGGGTTCTTA------AAGAAGAGTCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 14/64 (21.9%) +# Similarity: 14/64 (21.9%) +# Gaps: 45/64 (70.3%) +# Score: 37.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGA-GATCTACACTCTTTCCCTACACGA----- 44 + ||.|. |.| ||||. |.|| +FC12044_91407 1 ---------------ACAGTGGGT------TCTTA------AAGAAGAGT 23 + +Illumina_Pair 45 CGCTCTTCCGATCT 58 + || +FC12044_91407 24 CG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 68 +# Identity: 14/68 (20.6%) +# Similarity: 14/68 (20.6%) +# Gaps: 50/68 (73.5%) +# Score: 48.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGG---CATACGAGA----TCGGTCTCGGCATTCCTGCT 43 + .|||. || |.|| .||| ||| +FC12044_91407 1 ---ACAGT----GGGTTCTTA-AAGAAGAGTCG----------------- 25 + +Illumina_Pair 44 GAACCGCTCTTCCGATCT 61 + +FC12044_91407 26 ------------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 32/45 (71.1%) +# Score: 36.5 +# +# +#======================================= + +Illumina_Pair 1 ACAC-------TCTTTCCCTACACGA-----CGCTCTTCCGATCT 33 + || ||||. |.|| || +FC12044_91407 1 --ACAGTGGGTTCTTA------AAGAAGAGTCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 16/39 (41.0%) +# Score: 40.5 +# +# +#======================================= + +Illumina_Pair 1 -CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGA-TCT 37 + |.|| || .|||.|...||| || ||. +FC12044_91407 1 ACAGT---GG-GTTCTTAAAGAA----------GAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 12/35 (34.3%) +# Similarity: 12/35 (34.3%) +# Gaps: 19/35 (54.3%) +# Score: 48.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAACTCT----GAA----C- 26 + ||.||.|.. ||| ||| | +FC12044_91407 1 ---------ACAGTGGGT-TCTTAAAGAAGAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 15/36 (41.7%) +# Similarity: 15/36 (41.7%) +# Gaps: 21/36 (58.3%) +# Score: 67.0 +# +# +#======================================= + +Illumina_DpnI 1 ACA---GGTTC-------AGAGTTCTACAGTCCGAC 26 + ||| ||||| ||||| || +FC12044_91407 1 ACAGTGGGTTCTTAAAGAAGAGT---------CG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 11/35 (31.4%) +# Similarity: 11/35 (31.4%) +# Gaps: 24/35 (68.6%) +# Score: 42.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------C--AAGCAGAAGA--CGGCATACGA 21 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 7/36 (19.4%) +# Similarity: 7/36 (19.4%) +# Gaps: 26/36 (72.2%) +# Score: 24.5 +# +# +#======================================= + +Illumina_DpnI 1 ---------TCGTA------TGCCGTCTTCTGCTTG 21 + ||.|| .|.|| +FC12044_91407 1 ACAGTGGGTTCTTAAAGAAGAGTCG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 11/35 (31.4%) +# Similarity: 11/35 (31.4%) +# Gaps: 24/35 (68.6%) +# Score: 42.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------C--AAGCAGAAGA--CGGCATACGA 21 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 15/54 (27.8%) +# Similarity: 15/54 (27.8%) +# Gaps: 39/54 (72.2%) +# Score: 67.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACA---GGTTC-------AGAGTTCTACAGT 40 + ||| ||||| ||||| +FC12044_91407 1 -------------------ACAGTGGGTTCTTAAAGAAGAGT-------- 23 + +Illumina_DpnI 41 CCGA 44 + || +FC12044_91407 24 -CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 17/34 (50.0%) +# Similarity: 17/34 (50.0%) +# Gaps: 11/34 (32.4%) +# Score: 67.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACAGTCCGACGA-TC- 32 + |||| .|.|||||..|. ||.|| || +FC12044_91407 1 --ACAG----TGGGTTCTTAAA---GAAGAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 12/30 (40.0%) +# Similarity: 12/30 (40.0%) +# Gaps: 14/30 (46.7%) +# Score: 48.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAACTCT----GAA----C- 21 + ||.||.|.. ||| ||| | +FC12044_91407 1 ----ACAGTGGGT-TCTTAAAGAAGAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 24/39 (61.5%) +# Score: 67.0 +# +# +#======================================= + +Illumina_NlaI 1 ACA---GGTTC-------AGAGTTCTACAGTCCGACATG 29 + ||| ||||| ||||| || +FC12044_91407 1 ACAGTGGGTTCTTAAAGAAGAGT---------CG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 11/37 (29.7%) +# Similarity: 11/37 (29.7%) +# Gaps: 26/37 (70.3%) +# Score: 42.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------C--AAGCAGAAGA--CGGCATACGANN 23 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 7/36 (19.4%) +# Similarity: 7/36 (19.4%) +# Gaps: 26/36 (72.2%) +# Score: 24.5 +# +# +#======================================= + +Illumina_NlaI 1 ---------TCGTA------TGCCGTCTTCTGCTTG 21 + ||.|| .|.|| +FC12044_91407 1 ACAGTGGGTTCTTAAAGAAGAGTCG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 11/35 (31.4%) +# Similarity: 11/35 (31.4%) +# Gaps: 24/35 (68.6%) +# Score: 42.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------C--AAGCAGAAGA--CGGCATACGA 21 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 15/54 (27.8%) +# Similarity: 15/54 (27.8%) +# Gaps: 39/54 (72.2%) +# Score: 67.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACA---GGTTC-------AGAGTTCTACAGT 40 + ||| ||||| ||||| +FC12044_91407 1 -------------------ACAGTGGGTTCTTAAAGAAGAGT-------- 23 + +Illumina_NlaI 41 CCGA 44 + || +FC12044_91407 24 -CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 15/42 (35.7%) +# Similarity: 15/42 (35.7%) +# Gaps: 27/42 (64.3%) +# Score: 67.0 +# +# +#======================================= + +Illumina_NlaI 1 CCGACA---GGTTC-------AGAGTTCTACAGTCCGACATG 32 + ||| ||||| ||||| || +FC12044_91407 1 ---ACAGTGGGTTCTTAAAGAAGAGT---------CG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 11/35 (31.4%) +# Similarity: 11/35 (31.4%) +# Gaps: 24/35 (68.6%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Smal 1 ----------C--AAGCAGAAGA--CGGCATACGA 21 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 15/30 (50.0%) +# Similarity: 15/30 (50.0%) +# Gaps: 9/30 (30.0%) +# Score: 57.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGA--GTTCTACAGTCCGACGA-TC- 26 + .|||. |||||..|. ||.|| || +FC12044_91407 1 --ACAGTGGGTTCTTAAA---GAAGAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 7/38 (18.4%) +# Similarity: 7/38 (18.4%) +# Gaps: 29/38 (76.3%) +# Score: 25.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGCCGTCTTCTG------CTT--------GT-- 22 + .|.| ||| || +FC12044_91407 1 -------------ACAGTGGGTTCTTAAAGAAGAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 11/35 (31.4%) +# Similarity: 11/35 (31.4%) +# Gaps: 24/35 (68.6%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Smal 1 ----------C--AAGCAGAAGA--CGGCATACGA 21 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 15/54 (27.8%) +# Similarity: 15/54 (27.8%) +# Gaps: 39/54 (72.2%) +# Score: 67.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACA---GGTTC-------AGAGTTCTACAGT 40 + ||| ||||| ||||| +FC12044_91407 1 -------------------ACAGTGGGTTCTTAAAGAAGAGT-------- 23 + +Illumina_Smal 41 CCGA 44 + || +FC12044_91407 24 -CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 17/34 (50.0%) +# Similarity: 17/34 (50.0%) +# Gaps: 11/34 (32.4%) +# Score: 67.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACAGTCCGACGA-TC- 32 + |||| .|.|||||..|. ||.|| || +FC12044_91407 1 --ACAG----TGGGTTCTTAAA---GAAGAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 18/37 (48.6%) +# Similarity: 18/37 (48.6%) +# Gaps: 16/37 (43.2%) +# Score: 77.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTATGC-CGTCTT--CTGCTTG- 33 + |.|| ||.| || ||.||| | |||| +FC12044_91407 1 -AACG--AGGG-------GCGCGACTTGAC--CTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGA----CGC-TCTTCCGATCT--- 33 + | |||| ||| .||| ||.|| +FC12044_91407 1 -------------A-ACGAGGGGCGCGACTT--GACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 19/62 (30.6%) +# Similarity: 19/62 (30.6%) +# Gaps: 41/62 (66.1%) +# Score: 65.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATAC----GGCGACCACCGAGATCTACACTCTTTCCCTACACGACG 46 + .|| |||| ||| ||| ||| +FC12044_91407 1 -----AACGAGGGGCG-----CGA----------CTT---------GAC- 20 + +Illumina_Geno 47 CTCTTCCGATCT 58 + ||| |. +FC12044_91407 21 --CTT--GG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 18/37 (48.6%) +# Similarity: 18/37 (48.6%) +# Gaps: 15/37 (40.5%) +# Score: 64.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT--- 34 + |.| ||.| ||| .||| ||| ||.|| +FC12044_91407 1 --AAC-GAGG--GGC--GCGA---CTT--GACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGA----CGC-TCTTCCGATCT--- 33 + | |||| ||| .||| ||.|| +FC12044_91407 1 -------------A-ACGAGGGGCGCGACTT--GACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 15/33 (45.5%) +# Similarity: 15/33 (45.5%) +# Gaps: 9/33 (27.3%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG- 32 + |.| ||.|.||| |..|..|| ||..| +FC12044_91407 1 -AAC-GAGGGGCG---CGACTTGA---CCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGA----CGC-TCTTCCGATCT--- 33 + | |||| ||| .||| ||.|| +FC12044_91407 1 -------------A-ACGAGGGGCGCGACTT--GACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 19/62 (30.6%) +# Similarity: 19/62 (30.6%) +# Gaps: 41/62 (66.1%) +# Score: 65.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATAC----GGCGACCACCGAGATCTACACTCTTTCCCTACACGACG 46 + .|| |||| ||| ||| ||| +FC12044_91407 1 -----AACGAGGGGCG-----CGA----------CTT---------GAC- 20 + +Illumina_Pair 47 CTCTTCCGATCT 58 + ||| |. +FC12044_91407 21 --CTT--GG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 17/62 (27.4%) +# Similarity: 17/62 (27.4%) +# Gaps: 38/62 (61.3%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCAT-TCCTGCTGAACCG 49 + .||||| ||.|.||.|.| .||| ||. +FC12044_91407 1 ----------------AACGAG---GGGCGCGACTTGACCT--TGG---- 25 + +Illumina_Pair 50 CTCTTCCGATCT 61 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGA----CGC-TCTTCCGATCT--- 33 + | |||| ||| .||| ||.|| +FC12044_91407 1 -------------A-ACGAGGGGCGCGACTT--GACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 13/47 (27.7%) +# Similarity: 13/47 (27.7%) +# Gaps: 32/47 (68.1%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAAC------CGC-TCTTCCGATCT--- 37 + ||| ||| .||| ||.|| +FC12044_91407 1 --------------------AACGAGGGGCGCGACTT--GACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 15/31 (48.4%) +# Similarity: 15/31 (48.4%) +# Gaps: 11/31 (35.5%) +# Score: 56.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGT----CG-GACTGTAGAACTCTGAAC 26 + |.||. || ||| |.||.|| ||. +FC12044_91407 1 -AACGAGGGGCGCGAC--TTGACCT-TGG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 11/39 (28.2%) +# Similarity: 11/39 (28.2%) +# Gaps: 27/39 (69.2%) +# Score: 39.5 +# +# +#======================================= + +Illumina_DpnI 1 -AC-AGGTTCAGAGTTCTACAGTC-CGAC---------- 26 + || ||| |.| |||| +FC12044_91407 1 AACGAGG--------------GGCGCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 41.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGACGGCATACGA----------- 21 + |.| ||.| ||| .||| +FC12044_91407 1 --AAC-GAGG--GGC--GCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 60.0 +# +# +#======================================= + +Illumina_DpnI 1 -TCGT-ATGC-CGTCTT--CTGCTTG- 21 + .||. ..|| ||.||| | |||| +FC12044_91407 1 AACGAGGGGCGCGACTTGAC--CTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 41.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGACGGCATACGA----------- 21 + |.| ||.| ||| .||| +FC12044_91407 1 --AAC-GAGG--GGC--GCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 16/51 (31.4%) +# Similarity: 16/51 (31.4%) +# Gaps: 33/51 (64.7%) +# Score: 59.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATAC----GGCGACCACCGACAGGTTCAGAGTTCTACAGTCC---G 43 + .|| |||| |||| || |.|| | +FC12044_91407 1 -----AACGAGGGGCG-----CGAC---TT------------GACCTTGG 25 + +Illumina_DpnI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 14/41 (34.1%) +# Similarity: 14/41 (34.1%) +# Gaps: 25/41 (61.0%) +# Score: 42.5 +# +# +#======================================= + +Illumina_DpnI 1 --CGACAGGTTCAGAGTTCTACAGTC-CGAC--GATC---- 32 + || ||| |.| |||| ||.| +FC12044_91407 1 AACG--AGG--------------GGCGCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 12/30 (40.0%) +# Similarity: 12/30 (40.0%) +# Gaps: 14/30 (46.7%) +# Score: 43.0 +# +# +#======================================= + +Illumina_NlaI 1 -TCG--------GACTGTAGAACTCTGAAC 21 + .|| ||| |.||.|| ||. +FC12044_91407 1 AACGAGGGGCGCGAC--TTGACCT-TGG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 13/39 (33.3%) +# Similarity: 13/39 (33.3%) +# Gaps: 24/39 (61.5%) +# Score: 51.5 +# +# +#======================================= + +Illumina_NlaI 1 -AC-AGGTTCAGAGTTCTACAGTC-CGACATG------- 29 + || ||| |.| ||||.|| +FC12044_91407 1 AACGAGG--------------GGCGCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 14/31 (45.2%) +# Score: 37.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAG---ACGGCAT-AC----GANN 23 + |.| ||.| .||.|.| || | +FC12044_91407 1 --AAC-GAGGGGCGCGACTTGACCTTGG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 60.0 +# +# +#======================================= + +Illumina_NlaI 1 -TCGT-ATGC-CGTCTT--CTGCTTG- 21 + .||. ..|| ||.||| | |||| +FC12044_91407 1 AACGAGGGGCGCGACTTGAC--CTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 41.0 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGACGGCATACGA----------- 21 + |.| ||.| ||| .||| +FC12044_91407 1 --AAC-GAGG--GGC--GCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 16/51 (31.4%) +# Similarity: 16/51 (31.4%) +# Gaps: 33/51 (64.7%) +# Score: 59.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATAC----GGCGACCACCGACAGGTTCAGAGTTCTACAGTCC---G 43 + .|| |||| |||| || |.|| | +FC12044_91407 1 -----AACGAGGGGCG-----CGAC---TT------------GACCTTGG 25 + +Illumina_NlaI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 25/41 (61.0%) +# Score: 49.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGAC-AGGTTCAGAGTTCTACAGTC-CGACATG------- 32 + .|| ||| |.| ||||.|| +FC12044_91407 1 --AACGAGG--------------GGCGCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 41.0 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGACGGCATACGA----------- 21 + |.| ||.| ||| .||| +FC12044_91407 1 --AAC-GAGG--GGC--GCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 11/36 (30.6%) +# Similarity: 11/36 (30.6%) +# Gaps: 21/36 (58.3%) +# Score: 37.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTAC---AGTC-CGAC--GATC---- 26 + .|| .|.| |||| ||.| +FC12044_91407 1 -----------AACGAGGGGCGCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 7/27 (25.9%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Smal 1 -TCGT-ATGC-CGTCTT--CTGCTTGT 22 + .||. ..|| ||.||| | ||||. +FC12044_91407 1 AACGAGGGGCGCGACTTGAC--CTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 41.0 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGACGGCATACGA----------- 21 + |.| ||.| ||| .||| +FC12044_91407 1 --AAC-GAGG--GGC--GCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 16/51 (31.4%) +# Similarity: 16/51 (31.4%) +# Gaps: 33/51 (64.7%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATAC----GGCGACCACCGACAGGTTCAGAGTTCTACAGTCC---G 43 + .|| |||| |||| || |.|| | +FC12044_91407 1 -----AACGAGGGGCG-----CGAC---TT------------GACCTTGG 25 + +Illumina_Smal 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 14/41 (34.1%) +# Similarity: 14/41 (34.1%) +# Gaps: 25/41 (61.0%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Smal 1 --CGACAGGTTCAGAGTTCTACAGTC-CGAC--GATC---- 32 + || ||| |.| |||| ||.| +FC12044_91407 1 AACG--AGG--------------GGCGCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 11/44 (25.0%) +# Similarity: 11/44 (25.0%) +# Gaps: 30/44 (68.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTAT---GCCGTCTTCTG-----CTTG--- 33 + |.| .|| | |.| |||| +FC12044_91407 1 ---------------TTTCCCACC--C--CAGGAAGCCTTGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 88.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC---GACGCTCTTCCGATCT 33 + |||||| ||.| ||.|| ||| |..| +FC12044_91407 1 ------TTTCCC-ACCCCAGGAAGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 18/61 (29.5%) +# Similarity: 18/61 (29.5%) +# Gaps: 39/61 (63.9%) +# Score: 88.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACAC---GACGC 47 + |||||| ||.| ||.|| +FC12044_91407 1 -------------------------------TTTCCC-ACCCCAGGAAGC 18 + +Illumina_Geno 48 TCTTCCGATCT 58 + ||| |..| +FC12044_91407 19 -CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 27/43 (62.8%) +# Score: 48.5 +# +# +#======================================= + +Illumina_Geno 1 ---------CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT 34 + | |||.| ||| ||| |..| +FC12044_91407 1 TTTCCCACCC---CAGGA-----------AGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 88.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC---GACGCTCTTCCGATCT 33 + |||||| ||.| ||.|| ||| |..| +FC12044_91407 1 ------TTTCCC-ACCCCAGGAAGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 13/42 (31.0%) +# Similarity: 13/42 (31.0%) +# Gaps: 27/42 (64.3%) +# Score: 55.5 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTT-------CAGCAGGAATGCCGAG--- 32 + || | |||||| |||..| +FC12044_91407 1 --------------TTTCCCACCC--CAGGAA-GCCTTGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 88.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC---GACGCTCTTCCGATCT 33 + |||||| ||.| ||.|| ||| |..| +FC12044_91407 1 ------TTTCCC-ACCCCAGGAAGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 18/61 (29.5%) +# Similarity: 18/61 (29.5%) +# Gaps: 39/61 (63.9%) +# Score: 88.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACAC---GACGC 47 + |||||| ||.| ||.|| +FC12044_91407 1 -------------------------------TTTCCC-ACCCCAGGAAGC 18 + +Illumina_Pair 48 TCTTCCGATCT 58 + ||| |..| +FC12044_91407 19 -CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 68 +# Identity: 15/68 (22.1%) +# Similarity: 15/68 (22.1%) +# Gaps: 50/68 (73.5%) +# Score: 67.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACC-- 48 + .|||| | ||| +FC12044_91407 1 ----------------------------------TTTCC--C---ACCCC 11 + +Illumina_Pair 49 -----GCTCTTCCGATCT 61 + || ||| |..| +FC12044_91407 12 AGGAAGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 88.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC---GACGCTCTTCCGATCT 33 + |||||| ||.| ||.|| ||| |..| +FC12044_91407 1 ------TTTCCC-ACCCCAGGAAGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 15/44 (34.1%) +# Similarity: 15/44 (34.1%) +# Gaps: 26/44 (59.1%) +# Score: 67.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAACC-------GCTCTTCCGATCT 37 + .|||| | ||| || ||| |..| +FC12044_91407 1 ----------TTTCC--C---ACCCCAGGAAGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 38.5 +# +# +#======================================= + +Illumina_DpnI 1 GATCGT--------CGGACTGTAGAA--CTCTGAAC 26 + | | |.| ||| || ||.|| +FC12044_91407 1 -----TTTCCCACCC---CAG--GAAGCCT-TGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 43.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTAC-------AGTCC---GAC 26 + .||| |.|| || || ||| +FC12044_91407 1 ----TTTC------CCACCCCAGGAAG-CCTTGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_DpnI 1 ---------CAAGCAGAAGACGGCAT--ACGA 21 + | |||.|..| |.| || +FC12044_91407 1 TTTCCCACCC---CAGGAAGC--CTTGGAC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_DpnI 1 TCGTAT---GCCGTCTTCTG-----CTTG--- 21 + |.| .|| | |.| |||| +FC12044_91407 1 ---TTTCCCACC--C--CAGGAAGCCTTGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_DpnI 1 ---------CAAGCAGAAGACGGCAT--ACGA 21 + | |||.|..| |.| || +FC12044_91407 1 TTTCCCACCC---CAGGAAGC--CTTGGAC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 16/48 (33.3%) +# Similarity: 16/48 (33.3%) +# Gaps: 27/48 (56.2%) +# Score: 68.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTT-CAGAGTTCT---ACAGTCCGA 44 + .|.| ||||| .||||.. | || || +FC12044_91407 1 ----TTTC-----CCACC-CCAGGAAGC------CTTGGAC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 12/44 (27.3%) +# Similarity: 12/44 (27.3%) +# Gaps: 31/44 (70.5%) +# Score: 44.0 +# +# +#======================================= + +Illumina_DpnI 1 ---------CGACAGGTTCAGAGTTCTACAGTCC---GACGATC 32 + | ||||. || || ||| +FC12044_91407 1 TTTCCCACCC--CAGGA--AG-----------CCTTGGAC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 12/33 (36.4%) +# Similarity: 12/33 (36.4%) +# Gaps: 20/33 (60.6%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 --TC--------GGA--CTGTAGAACTCTGAAC 21 + || ||| | || ||.|| +FC12044_91407 1 TTTCCCACCCCAGGAAGC-------CT-TGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 13/38 (34.2%) +# Similarity: 13/38 (34.2%) +# Gaps: 22/38 (57.9%) +# Score: 44.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAGTCC---GA---CATG--- 29 + .||| |.|| || || |.|| +FC12044_91407 1 ----TTTC------CCAC---CCCAGGAAGCCTTGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 20/34 (58.8%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 ---------CAAGCAGAAGACGGCAT--ACGANN 23 + | |||.|..| |.| || +FC12044_91407 1 TTTCCCACCC---CAGGAAGC--CTTGGAC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGTAT---GCCGTCTTCTG-----CTTG--- 21 + |.| .|| | |.| |||| +FC12044_91407 1 ---TTTCCCACC--C--CAGGAAGCCTTGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 ---------CAAGCAGAAGACGGCAT--ACGA 21 + | |||.|..| |.| || +FC12044_91407 1 TTTCCCACCC---CAGGAAGC--CTTGGAC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 16/48 (33.3%) +# Similarity: 16/48 (33.3%) +# Gaps: 27/48 (56.2%) +# Score: 68.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTT-CAGAGTTCT---ACAGTCCGA 44 + .|.| ||||| .||||.. | || || +FC12044_91407 1 ----TTTC-----CCACC-CCAGGAAGC------CTTGGAC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 29/43 (67.4%) +# Score: 54.0 +# +# +#======================================= + +Illumina_NlaI 1 --------CCGACAGGTTCAGAGTTCTACAGTCC---GACATG 32 + || ||||. || || ||| +FC12044_91407 1 TTTCCCACCC--CAGGA--AG-----------CCTTGGAC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_Smal 1 ---------CAAGCAGAAGACGGCAT--ACGA 21 + | |||.|..| |.| || +FC12044_91407 1 TTTCCCACCC---CAGGAAGC--CTTGGAC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTAC-------AGTCC---GACGATC 26 + .||| |.|| || || ||| +FC12044_91407 1 TTTC------CCACCCCAGGAAG-CCTTGGAC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 23/35 (65.7%) +# Score: 38.5 +# +# +#======================================= + +Illumina_Smal 1 --TC--------GTATGCC---GTCTTCTGCTTGT 22 + || |.|.||| |.| +FC12044_91407 1 TTTCCCACCCCAGGAAGCCTTGGAC---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_Smal 1 ---------CAAGCAGAAGACGGCAT--ACGA 21 + | |||.|..| |.| || +FC12044_91407 1 TTTCCCACCC---CAGGAAGC--CTTGGAC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 16/48 (33.3%) +# Similarity: 16/48 (33.3%) +# Gaps: 27/48 (56.2%) +# Score: 68.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTT-CAGAGTTCT---ACAGTCCGA 44 + .|.| ||||| .||||.. | || || +FC12044_91407 1 ----TTTC-----CCACC-CCAGGAAGC------CTTGGAC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 12/44 (27.3%) +# Similarity: 12/44 (27.3%) +# Gaps: 31/44 (70.5%) +# Score: 44.0 +# +# +#======================================= + +Illumina_Smal 1 ---------CGACAGGTTCAGAGTTCTACAGTCC---GACGATC 32 + | ||||. || || ||| +FC12044_91407 1 TTTCCCACCC--CAGGA--AG-----------CCTTGGAC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 17/37 (45.9%) +# Similarity: 17/37 (45.9%) +# Gaps: 16/37 (43.2%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAG-CT-CGTATGCC--GTCTTCTGCTTG 33 + || || || || |||| |.|..||.| +FC12044_91407 1 --TC-----AGCCTCCG--TGCCCAGCCCACTCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Geno 1 ACA--CT-CTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| || |.|.|||..|.| || ||| +FC12044_91407 1 TCAGCCTCCGTGCCCAGCCC-AC-----TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 58 +# Identity: 18/58 (31.0%) +# Similarity: 18/58 (31.0%) +# Gaps: 33/58 (56.9%) +# Score: 98.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT 50 + .|.| ||.|||.| |||..|.| || +FC12044_91407 1 ------TCAG---CCTCCGTG-------------CCCAGCCC-AC----- 22 + +Illumina_Geno 51 TCCGATCT 58 + ||| +FC12044_91407 23 TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 14/36 (38.9%) +# Similarity: 14/36 (38.9%) +# Gaps: 13/36 (36.1%) +# Score: 68.0 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT-- 34 + .|.||.|.||.||.|..||.| || +FC12044_91407 1 ----------TCAGCCTCCGTGCCCAGCCCA-CTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Geno 1 ACA--CT-CTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| || |.|.|||..|.| || ||| +FC12044_91407 1 TCAGCCTCCGTGCCCAGCCC-AC-----TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 12/43 (27.9%) +# Similarity: 12/43 (27.9%) +# Gaps: 29/43 (67.4%) +# Score: 53.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAG---CAGGAATGCCGAG-------- 32 + |||| |.| ||||.|| +FC12044_91407 1 ---------------TCAGCCTCCG---TGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Pair 1 ACA--CT-CTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| || |.|.|||..|.| || ||| +FC12044_91407 1 TCAGCCTCCGTGCCCAGCCC-AC-----TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 58 +# Identity: 18/58 (31.0%) +# Similarity: 18/58 (31.0%) +# Gaps: 33/58 (56.9%) +# Score: 98.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT 50 + .|.| ||.|||.| |||..|.| || +FC12044_91407 1 ------TCAG---CCTCCGTG-------------CCCAGCCC-AC----- 22 + +Illumina_Pair 51 TCCGATCT 58 + ||| +FC12044_91407 23 TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 63 +# Identity: 19/63 (30.2%) +# Similarity: 19/63 (30.2%) +# Gaps: 40/63 (63.5%) +# Score: 99.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCT-CG-GCATTCCTGCTGAACC 48 + ||.|.|| || || ||.|| || +FC12044_91407 1 -----------------------TCAGCCTCCGTGC---CCAGC----CC 20 + +Illumina_Pair 49 GCTCTTCCGATCT 61 + .||| | +FC12044_91407 21 ACTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Pair 1 ACA--CT-CTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| || |.|.|||..|.| || ||| +FC12044_91407 1 TCAGCCTCCGTGCCCAGCCC-AC-----TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 18/40 (45.0%) +# Similarity: 18/40 (45.0%) +# Gaps: 18/40 (45.0%) +# Score: 92.0 +# +# +#======================================= + +Illumina_Pair 1 -CGGTCT-CG-GCATTCCTGCTGAACCGCTCTTCCGATCT 37 + |.|.|| || || ||.|| ||.||| | +FC12044_91407 1 TCAGCCTCCGTGC---CCAGC----CCACTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 14/34 (41.2%) +# Similarity: 14/34 (41.2%) +# Gaps: 17/34 (50.0%) +# Score: 50.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACT--GT----AG--AACTCTGAAC 26 + ||.|.|| || || .|||| | +FC12044_91407 1 -----TCAGCCTCCGTGCCCAGCCCACTC----C 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 14/34 (41.2%) +# Similarity: 14/34 (41.2%) +# Gaps: 17/34 (50.0%) +# Score: 53.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAG-----GTTCAGAGTTCTACAGTCCGAC--- 26 + .||| ||.| | |||.|| || +FC12044_91407 1 TCAGCCTCCGTGC------C--CAGCCC-ACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 6/35 (17.1%) +# Similarity: 6/35 (17.1%) +# Gaps: 24/35 (68.6%) +# Score: 33.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGACGGCATACGA-------------- 21 + .|.||.|.||. +FC12044_91407 1 ----------TCAGCCTCCGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 43.0 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGCCGTCTTCTGCTTG------------- 21 + || .||| || |.|| +FC12044_91407 1 TC---AGCC-TC-----CGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 6/35 (17.1%) +# Similarity: 6/35 (17.1%) +# Gaps: 24/35 (68.6%) +# Score: 33.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGACGGCATACGA-------------- 21 + .|.||.|.||. +FC12044_91407 1 ----------TCAGCCTCCGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 17/47 (36.2%) +# Similarity: 17/47 (36.2%) +# Gaps: 25/47 (53.2%) +# Score: 78.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGA---CAGGTTCAGAGTTCTACAGTCCGA 44 + .|.| ||.|||. ||| | |.|| ||| +FC12044_91407 1 ------TCAG---CCTCCGTGCCCAG---C------CCAC--TCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 16/38 (42.1%) +# Similarity: 16/38 (42.1%) +# Gaps: 19/38 (50.0%) +# Score: 59.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAG-----GTTCAGAGTTCTACAGTCCGACGATC- 32 + .||| ||.| | |||.|| || || +FC12044_91407 1 --TCAGCCTCCGTGC------C--CAGCCC-AC--TCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 14/29 (48.3%) +# Similarity: 14/29 (48.3%) +# Gaps: 12/29 (41.4%) +# Score: 50.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACT--GT----AG--AACTCTGAAC 21 + ||.|.|| || || .|||| | +FC12044_91407 1 TCAGCCTCCGTGCCCAGCCCACTC----C 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 12/42 (28.6%) +# Similarity: 12/42 (28.6%) +# Gaps: 30/42 (71.4%) +# Score: 51.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAGTCCGACATG------------- 29 + |||| | | |||| || +FC12044_91407 1 ------TCAG----C--C--TCCG---TGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 9/37 (24.3%) +# Similarity: 9/37 (24.3%) +# Gaps: 26/37 (70.3%) +# Score: 30.0 +# +# +#======================================= + +Illumina_NlaI 1 -------------CAAGCAGAAGACGGCA-TACGANN 23 + |.||| | || |.| +FC12044_91407 1 TCAGCCTCCGTGCCCAGC------C--CACTCC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 43.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGCCGTCTTCTGCTTG------------- 21 + || .||| || |.|| +FC12044_91407 1 TC---AGCC-TC-----CGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 6/35 (17.1%) +# Similarity: 6/35 (17.1%) +# Gaps: 24/35 (68.6%) +# Score: 33.0 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGACGGCATACGA-------------- 21 + .|.||.|.||. +FC12044_91407 1 ----------TCAGCCTCCGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 17/47 (36.2%) +# Similarity: 17/47 (36.2%) +# Gaps: 25/47 (53.2%) +# Score: 78.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGA---CAGGTTCAGAGTTCTACAGTCCGA 44 + .|.| ||.|||. ||| | |.|| ||| +FC12044_91407 1 ------TCAG---CCTCCGTGCCCAG---C------CCAC--TCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 13/42 (31.0%) +# Similarity: 13/42 (31.0%) +# Gaps: 27/42 (64.3%) +# Score: 57.0 +# +# +#======================================= + +Illumina_NlaI 1 -------CCGA---CAGGTTCAGAGTTCTACAGTCCGACATG 32 + |||. ||| | |.|| ||| +FC12044_91407 1 TCAGCCTCCGTGCCCAG---C------CCAC--TCC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 6/35 (17.1%) +# Similarity: 6/35 (17.1%) +# Gaps: 24/35 (68.6%) +# Score: 33.0 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGACGGCATACGA-------------- 21 + .|.||.|.||. +FC12044_91407 1 ----------TCAGCCTCCGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 16/31 (51.6%) +# Similarity: 16/31 (51.6%) +# Gaps: 11/31 (35.5%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Smal 1 GTTCAG----AGTTCTACAGTCCGACGATC- 26 + |||| .||.| .|||.|| || || +FC12044_91407 1 --TCAGCCTCCGTGC-CCAGCCC-AC--TCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 15/31 (48.4%) +# Score: 42.0 +# +# +#======================================= + +Illumina_Smal 1 ------T-CGTATGCC--GTCTTCTGCTTGT 22 + | || |||| |.|..||.| +FC12044_91407 1 TCAGCCTCCG--TGCCCAGCCCACTCC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 6/35 (17.1%) +# Similarity: 6/35 (17.1%) +# Gaps: 24/35 (68.6%) +# Score: 33.0 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGACGGCATACGA-------------- 21 + .|.||.|.||. +FC12044_91407 1 ----------TCAGCCTCCGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 17/47 (36.2%) +# Similarity: 17/47 (36.2%) +# Gaps: 25/47 (53.2%) +# Score: 78.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGA---CAGGTTCAGAGTTCTACAGTCCGA 44 + .|.| ||.|||. ||| | |.|| ||| +FC12044_91407 1 ------TCAG---CCTCCGTGCCCAG---C------CCAC--TCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 16/38 (42.1%) +# Similarity: 16/38 (42.1%) +# Gaps: 19/38 (50.0%) +# Score: 59.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAG-----GTTCAGAGTTCTACAGTCCGACGATC- 32 + .||| ||.| | |||.|| || || +FC12044_91407 1 --TCAGCCTCCGTGC------C--CAGCCC-AC--TCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 12/43 (27.9%) +# Similarity: 12/43 (27.9%) +# Gaps: 28/43 (65.1%) +# Score: 44.0 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCT----C------GTATGCCGTCTTCTGCTTG 33 + .||||.|| ||| | ||. +FC12044_91407 1 -CTCGGGAG-GCTGAGGCAGGGGGGTT---------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 10/46 (21.7%) +# Similarity: 10/46 (21.7%) +# Gaps: 34/46 (73.9%) +# Score: 34.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC--GACGCT----C-------TTCCGATCT 33 + |.| ||.||| | || +FC12044_91407 1 --------------CTCGGGAGGCTGAGGCAGGGGGGTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 71 +# Identity: 10/71 (14.1%) +# Similarity: 10/71 (14.1%) +# Gaps: 59/71 (83.1%) +# Score: 34.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACAC--GACGCT 48 + |.| ||.||| +FC12044_91407 1 ---------------------------------------CTCGGGAGGCT 11 + +Illumina_Geno 49 ----C-------TTCCGATCT 58 + | || +FC12044_91407 12 GAGGCAGGGGGGTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 14/37 (37.8%) +# Similarity: 14/37 (37.8%) +# Gaps: 15/37 (40.5%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGAC---GGCATACGAGCTCTTCCGATCT 34 + |. |.|.||.| ||||...|.| || +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGG---TT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 10/46 (21.7%) +# Similarity: 10/46 (21.7%) +# Gaps: 34/46 (73.9%) +# Score: 34.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC--GACGCT----C-------TTCCGATCT 33 + |.| ||.||| | || +FC12044_91407 1 --------------CTCGGGAGGCTGAGGCAGGGGGGTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 16/35 (45.7%) +# Similarity: 16/35 (45.7%) +# Gaps: 13/35 (37.1%) +# Score: 57.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG--- 32 + .||||.|| ||.|. .||||| |.| +FC12044_91407 1 -CTCGGGAG-GCTGA--GGCAGG------GGGGTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 10/46 (21.7%) +# Similarity: 10/46 (21.7%) +# Gaps: 34/46 (73.9%) +# Score: 34.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC--GACGCT----C-------TTCCGATCT 33 + |.| ||.||| | || +FC12044_91407 1 --------------CTCGGGAGGCTGAGGCAGGGGGGTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 71 +# Identity: 10/71 (14.1%) +# Similarity: 10/71 (14.1%) +# Gaps: 59/71 (83.1%) +# Score: 34.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACAC--GACGCT 48 + |.| ||.||| +FC12044_91407 1 ---------------------------------------CTCGGGAGGCT 11 + +Illumina_Pair 49 ----C-------TTCCGATCT 58 + | || +FC12044_91407 12 GAGGCAGGGGGGTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 69 +# Identity: 14/69 (20.3%) +# Similarity: 14/69 (20.3%) +# Gaps: 52/69 (75.4%) +# Score: 50.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGC 50 + |||||.| .|||||. || +FC12044_91407 1 ----------------------------CTCGGGA----GGCTGAG--GC 16 + +Illumina_Pair 51 --------TCTTCCGATCT 61 + | +FC12044_91407 17 AGGGGGGTT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 10/46 (21.7%) +# Similarity: 10/46 (21.7%) +# Gaps: 34/46 (73.9%) +# Score: 34.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC--GACGCT----C-------TTCCGATCT 33 + |.| ||.||| | || +FC12044_91407 1 --------------CTCGGGAGGCTGAGGCAGGGGGGTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 14/45 (31.1%) +# Similarity: 14/45 (31.1%) +# Gaps: 28/45 (62.2%) +# Score: 50.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAACCGC--------TCTTCCGATCT 37 + |||||.| .|||||. || | +FC12044_91407 1 ----CTCGGGA----GGCTGAG--GCAGGGGGGTT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 12/36 (33.3%) +# Similarity: 12/36 (33.3%) +# Gaps: 21/36 (58.3%) +# Score: 42.5 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAACTCTGA-AC--------- 26 + | |||| |..| |||| .| +FC12044_91407 1 ---C-TCGG---GAGG----CTGAGGCAGGGGGGTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 8/40 (20.0%) +# Similarity: 8/40 (20.0%) +# Gaps: 29/40 (72.5%) +# Score: 37.0 +# +# +#======================================= + +Illumina_DpnI 1 --------------ACAGGTTCAGAGTTCTACAGTCCGAC 26 + .|||| .|.||| +FC12044_91407 1 CTCGGGAGGCTGAGGCAGG---GGGGTT------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 41.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGAC---GGCA-------TACGA 21 + |. |.|.||.| |||| | +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGGTT---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 27.0 +# +# +#======================================= + +Illumina_DpnI 1 -TCGTATGCCGTCTTCT---GC-------TTG 21 + |||...| | || || || +FC12044_91407 1 CTCGGGAG--G----CTGAGGCAGGGGGGTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 41.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGAC---GGCA-------TACGA 21 + |. |.|.||.| |||| | +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGGTT---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 15/51 (29.4%) +# Similarity: 15/51 (29.4%) +# Gaps: 33/51 (64.7%) +# Score: 52.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCG 43 + | ||| || |.|||| .|.||| +FC12044_91407 1 -------CTCGGGAGGCTGA-----GGCAGG---GGGGTT---------- 25 + +Illumina_DpnI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 23/40 (57.5%) +# Score: 42.5 +# +# +#======================================= + +Illumina_DpnI 1 --CGACAGGTTCAGAGTTCTACAG------TCCGACGATC 32 + ||..||| |.||| .||| | +FC12044_91407 1 CTCGGGAGG--CTGAG----GCAGGGGGGTT--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 42.5 +# +# +#======================================= + +Illumina_NlaI 1 -TCGGACTGTAGAACTCTGA-AC--------- 21 + |||| |..| |||| .| +FC12044_91407 1 CTCGG---GAGG----CTGAGGCAGGGGGGTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 8/43 (18.6%) +# Similarity: 8/43 (18.6%) +# Gaps: 32/43 (74.4%) +# Score: 37.0 +# +# +#======================================= + +Illumina_NlaI 1 --------------ACAGGTTCAGAGTTCTACAGTCCGACATG 29 + .|||| .|.||| +FC12044_91407 1 CTCGGGAGGCTGAGGCAGG---GGGGTT--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 11/33 (33.3%) +# Similarity: 11/33 (33.3%) +# Gaps: 18/33 (54.5%) +# Score: 41.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGAC---GGCA-------TACGANN 23 + |. |.|.||.| |||| | +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGGTT------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 27.0 +# +# +#======================================= + +Illumina_NlaI 1 -TCGTATGCCGTCTTCT---GC-------TTG 21 + |||...| | || || || +FC12044_91407 1 CTCGGGAG--G----CTGAGGCAGGGGGGTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 41.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGAC---GGCA-------TACGA 21 + |. |.|.||.| |||| | +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGGTT---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 15/51 (29.4%) +# Similarity: 15/51 (29.4%) +# Gaps: 33/51 (64.7%) +# Score: 52.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCG 43 + | ||| || |.|||| .|.||| +FC12044_91407 1 -------CTCGGGAGGCTGA-----GGCAGG---GGGGTT---------- 25 + +Illumina_NlaI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 29/43 (67.4%) +# Score: 42.5 +# +# +#======================================= + +Illumina_NlaI 1 ---------CCGA--CAGGTTCAGAGTTCTACAGTCCGACATG 32 + |.|| |||| .|.||| +FC12044_91407 1 CTCGGGAGGCTGAGGCAGG---GGGGTT--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 41.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGAC---GGCA-------TACGA 21 + |. |.|.||.| |||| | +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGGTT---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 9/40 (22.5%) +# Similarity: 9/40 (22.5%) +# Gaps: 29/40 (72.5%) +# Score: 25.5 +# +# +#======================================= + +Illumina_Smal 1 --------GTTCAGAGTTCTACAG------TCCGACGATC 26 + | |.||| .||| | +FC12044_91407 1 CTCGGGAGG--CTGAG----GCAGGGGGGTT--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 11/33 (33.3%) +# Similarity: 11/33 (33.3%) +# Gaps: 19/33 (57.6%) +# Score: 27.0 +# +# +#======================================= + +Illumina_Smal 1 -TCGTATGCCGTCTTCT---GC-------TTGT 22 + |||...| | || || || +FC12044_91407 1 CTCGGGAG--G----CTGAGGCAGGGGGGTT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 41.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGAC---GGCA-------TACGA 21 + |. |.|.||.| |||| | +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGGTT---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 15/51 (29.4%) +# Similarity: 15/51 (29.4%) +# Gaps: 33/51 (64.7%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCG 43 + | ||| || |.|||| .|.||| +FC12044_91407 1 -------CTCGGGAGGCTGA-----GGCAGG---GGGGTT---------- 25 + +Illumina_Smal 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 23/40 (57.5%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Smal 1 --CGACAGGTTCAGAGTTCTACAG------TCCGACGATC 32 + ||..||| |.||| .||| | +FC12044_91407 1 CTCGGGAGG--CTGAG----GCAGGGGGGTT--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 16/40 (40.0%) +# Similarity: 16/40 (40.0%) +# Gaps: 22/40 (55.0%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Geno 1 ---GATC-GGAA--G-AGCTCGTATGCCGTCTTCTGCTTG 33 + .||| .||| | ||||| || || +FC12044_91407 1 CCAAATCTTGAATTGTAGCTC-----CC--CT-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 22/40 (55.0%) +# Score: 62.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC-------GACGCTCTTCCGATCT 33 + ||.|..| |..|||| || || +FC12044_91407 1 ----------CCAAATCTTGAATTGTAGCTC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 65 +# Identity: 15/65 (23.1%) +# Similarity: 15/65 (23.1%) +# Gaps: 47/65 (72.3%) +# Score: 80.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACA-------CTCTTTCCCTACACG 43 + || |.||||..| ||| |||| +FC12044_91407 1 ----------------CC-AAATCTTGAATTGTAGCTC---CCCT----- 25 + +Illumina_Geno 44 ACGCTCTTCCGATCT 58 + +FC12044_91407 26 --------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 17/39 (43.6%) +# Similarity: 17/39 (43.6%) +# Gaps: 19/39 (48.7%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Geno 1 -CAAG-C-AGAA--GACGGCATACGAGCTCTTCCGATCT 34 + |||. | .||| |. ||||| || || +FC12044_91407 1 CCAAATCTTGAATTGT---------AGCTC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 22/40 (55.0%) +# Score: 62.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC-------GACGCTCTTCCGATCT 33 + ||.|..| |..|||| || || +FC12044_91407 1 ----------CCAAATCTTGAATTGTAGCTC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 21/39 (53.8%) +# Score: 37.0 +# +# +#======================================= + +Illumina_Pair 1 ---GATC-GGAA--G-AGCGGTTCAGCAGGAATGCCGAG 32 + .||| .||| | ||| || | ||. +FC12044_91407 1 CCAAATCTTGAATTGTAGC---TC--C-------CCT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 22/40 (55.0%) +# Score: 62.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC-------GACGCTCTTCCGATCT 33 + ||.|..| |..|||| || || +FC12044_91407 1 ----------CCAAATCTTGAATTGTAGCTC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 65 +# Identity: 15/65 (23.1%) +# Similarity: 15/65 (23.1%) +# Gaps: 47/65 (72.3%) +# Score: 80.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACA-------CTCTTTCCCTACACG 43 + || |.||||..| ||| |||| +FC12044_91407 1 ----------------CC-AAATCTTGAATTGTAGCTC---CCCT----- 25 + +Illumina_Pair 44 ACGCTCTTCCGATCT 58 + +FC12044_91407 26 --------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 21/61 (34.4%) +# Similarity: 21/61 (34.4%) +# Gaps: 36/61 (59.0%) +# Score: 74.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGC 50 + | ||.| |||.| |.| ||.| || +FC12044_91407 1 -----------C--CAAA---------TCTTG--AAT-----TGTA--GC 19 + +Illumina_Pair 51 TCTTCCGATCT 61 + || || || +FC12044_91407 20 TC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 22/40 (55.0%) +# Score: 62.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC-------GACGCTCTTCCGATCT 33 + ||.|..| |..|||| || || +FC12044_91407 1 ----------CCAAATCTTGAATTGTAGCTC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 17/41 (41.5%) +# Similarity: 17/41 (41.5%) +# Gaps: 20/41 (48.8%) +# Score: 66.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGC--ATTCCTGC--TGAACCGCTCTTCCGATCT 37 + | |.||.||. ||.| |||| || || +FC12044_91407 1 ---------CCAAATCTTGAATTGTA--GCTC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 15/32 (46.9%) +# Similarity: 15/32 (46.9%) +# Gaps: 13/32 (40.6%) +# Score: 61.0 +# +# +#======================================= + +Illumina_DpnI 1 ---GATCGTCGGACTGTAGAACTCTGAAC--- 26 + .|||.| |.|.||||| ||| | +FC12044_91407 1 CCAAATCTT-GAATTGTAG--CTC----CCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 9/30 (30.0%) +# Score: 49.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTC-AGAGTTCTA-CAGTCCGAC-- 26 + |....|| .||.||.|| | ||| | +FC12044_91407 1 -CCAAATCTTGAATTGTAGC--TCC--CCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 8/35 (22.9%) +# Similarity: 8/35 (22.9%) +# Gaps: 24/35 (68.6%) +# Score: 23.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGACGGCATACGA-------------- 21 + | ||.|. |.| || +FC12044_91407 1 C---CAAAT-----CTT--GAATTGTAGCTCCCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 8/37 (21.6%) +# Similarity: 8/37 (21.6%) +# Gaps: 28/37 (75.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGCC--GTCTT--------------CTGCTTG 21 + || .|||| || +FC12044_91407 1 -------CCAAATCTTGAATTGTAGCTCCCCT----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 8/35 (22.9%) +# Similarity: 8/35 (22.9%) +# Gaps: 24/35 (68.6%) +# Score: 23.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGACGGCATACGA-------------- 21 + | ||.|. |.| || +FC12044_91407 1 C---CAAAT-----CTT--GAATTGTAGCTCCCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 49 +# Identity: 14/49 (28.6%) +# Similarity: 14/49 (28.6%) +# Gaps: 29/49 (59.2%) +# Score: 54.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTC-AGAGTT----CTACAGTCCGA 44 + ||.| .|| .||.|| ||.| ||. +FC12044_91407 1 ----------------CCAA----ATCTTGAATTGTAGCTCC---CCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 17/35 (48.6%) +# Similarity: 17/35 (48.6%) +# Gaps: 13/35 (37.1%) +# Score: 51.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTC-AGAGTTCTA-CAGTCCGACGATC- 32 + | || ..|| .||.||.|| | ||| | | +FC12044_91407 1 C--CA-AATCTTGAATTGTAGC--TCC--C---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 13/31 (41.9%) +# Similarity: 13/31 (41.9%) +# Gaps: 16/31 (51.6%) +# Score: 55.5 +# +# +#======================================= + +Illumina_NlaI 1 -----TC--GGACTGTAGAACTCTGAAC--- 21 + || |.|.||||| ||| | +FC12044_91407 1 CCAAATCTTGAATTGTAG--CTC----CCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 15/31 (48.4%) +# Similarity: 15/31 (48.4%) +# Gaps: 8/31 (25.8%) +# Score: 50.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTC-AGAGTTCTA-CAGTCCGACATG 29 + |....|| .||.||.|| | ||| |.| +FC12044_91407 1 -CCAAATCTTGAATTGTAGC--TCC--CCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 11/33 (33.3%) +# Similarity: 11/33 (33.3%) +# Gaps: 18/33 (54.5%) +# Score: 20.0 +# +# +#======================================= + +Illumina_NlaI 1 -CAAG-C-AGAA--GA-----CGGCATACGANN 23 + |||. | .||| |. | |.| +FC12044_91407 1 CCAAATCTTGAATTGTAGCTCC--CCT------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 8/37 (21.6%) +# Similarity: 8/37 (21.6%) +# Gaps: 28/37 (75.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGCC--GTCTT--------------CTGCTTG 21 + || .|||| || +FC12044_91407 1 -------CCAAATCTTGAATTGTAGCTCCCCT----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 8/35 (22.9%) +# Similarity: 8/35 (22.9%) +# Gaps: 24/35 (68.6%) +# Score: 23.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGACGGCATACGA-------------- 21 + | ||.|. |.| || +FC12044_91407 1 C---CAAAT-----CTT--GAATTGTAGCTCCCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 49 +# Identity: 14/49 (28.6%) +# Similarity: 14/49 (28.6%) +# Gaps: 29/49 (59.2%) +# Score: 54.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTC-AGAGTT----CTACAGTCCGA 44 + ||.| .|| .||.|| ||.| ||. +FC12044_91407 1 ----------------CCAA----ATCTTGAATTGTAGCTCC---CCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 17/34 (50.0%) +# Similarity: 17/34 (50.0%) +# Gaps: 11/34 (32.4%) +# Score: 62.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTC-AGAGTTCTA-CAGTCCGACATG 32 + ||.| .|| .||.||.|| | ||| |.| +FC12044_91407 1 CCAA----ATCTTGAATTGTAGC--TCC--CCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 8/35 (22.9%) +# Similarity: 8/35 (22.9%) +# Gaps: 24/35 (68.6%) +# Score: 23.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGACGGCATACGA-------------- 21 + | ||.|. |.| || +FC12044_91407 1 C---CAAAT-----CTT--GAATTGTAGCTCCCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 14/32 (43.8%) +# Similarity: 14/32 (43.8%) +# Gaps: 13/32 (40.6%) +# Score: 46.0 +# +# +#======================================= + +Illumina_Smal 1 ---GTTC-AGAGTTCTA-CAGTCCGACGATC- 26 + ..|| .||.||.|| | ||| | | +FC12044_91407 1 CCAAATCTTGAATTGTAGC--TCC--C---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 21/34 (61.8%) +# Score: 45.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGCC--GTCTTCTG-CTTGT--------- 22 + || .||| || .|||| +FC12044_91407 1 -------CCAAATCT--TGAATTGTAGCTCCCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 8/35 (22.9%) +# Similarity: 8/35 (22.9%) +# Gaps: 24/35 (68.6%) +# Score: 23.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGACGGCATACGA-------------- 21 + | ||.|. |.| || +FC12044_91407 1 C---CAAAT-----CTT--GAATTGTAGCTCCCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 49 +# Identity: 14/49 (28.6%) +# Similarity: 14/49 (28.6%) +# Gaps: 29/49 (59.2%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTC-AGAGTT----CTACAGTCCGA 44 + ||.| .|| .||.|| ||.| ||. +FC12044_91407 1 ----------------CCAA----ATCTTGAATTGTAGCTCC---CCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 17/35 (48.6%) +# Similarity: 17/35 (48.6%) +# Gaps: 13/35 (37.1%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTC-AGAGTTCTA-CAGTCCGACGATC- 32 + | || ..|| .||.||.|| | ||| | | +FC12044_91407 1 C--CA-AATCTTGAATTGTAGC--TCC--C---CT 25 + + +#--------------------------------------- +#--------------------------------------- diff --git a/tools/emboss_5/test-data/emboss_needleall_out.score b/tools/emboss_5/test-data/emboss_needleall_out.score new file mode 100644 index 00000000000..db94d49b970 --- /dev/null +++ b/tools/emboss_5/test-data/emboss_needleall_out.score @@ -0,0 +1,777 @@ +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_406_24 38 (21.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_406_24 39 (24.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_406_24 60 (31.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_406_24 44 (25.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_406_24 39 (24.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_406_24 49 (16.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_406_24 39 (24.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_406_24 60 (31.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_406_24 67 (21.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_406_24 39 (24.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_406_24 43 (21.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_406_24 38 (14.5) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_406_24 40 (24.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_406_24 29 (12.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_406_24 30 (12.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_406_24 29 (12.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_406_24 45 (41.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_406_24 42 (23.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_406_24 34 (12.5) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_406_24 40 (27.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_406_24 31 (12.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_406_24 30 (12.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_406_24 29 (12.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_406_24 45 (41.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_406_24 40 (27.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_406_24 29 (12.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_406_24 36 (23.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_406_24 31 (13.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_406_24 29 (12.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_406_24 45 (41.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_406_24 42 (23.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_720_610 44 (17.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_720_610 40 (31.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_720_610 61 (31.5) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_720_610 47 (20.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_720_610 40 (31.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_720_610 40 (31.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_720_610 61 (31.5) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_720_610 64 (33.5) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_720_610 40 (31.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_720_610 40 (33.5) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_720_610 43 (20.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_720_610 40 (9.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_720_610 36 (11.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_720_610 39 (15.5) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_720_610 36 (11.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_720_610 59 (10.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_720_610 44 (15.0) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_720_610 38 (20.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_720_610 42 (9.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_720_610 36 (7.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_720_610 39 (15.5) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_720_610 36 (11.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_720_610 59 (10.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_720_610 45 (9.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_720_610 36 (11.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_720_610 38 (15.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_720_610 42 (15.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_720_610 36 (11.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_720_610 59 (10.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_720_610 44 (15.0) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_345_133 47 (8.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_345_133 53 (16.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_345_133 69 (20.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_345_133 45 (21.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_345_133 53 (16.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_345_133 54 (6.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_345_133 53 (16.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_345_133 69 (20.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_345_133 72 (21.5) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_345_133 53 (16.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_345_133 57 (16.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_345_133 33 (27.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_345_133 40 (19.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_345_133 32 (21.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_345_133 41 (7.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_345_133 32 (21.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_345_133 56 (16.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_345_133 37 (16.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_345_133 28 (27.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_345_133 34 (24.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_345_133 34 (21.5) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_345_133 41 (7.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_345_133 32 (21.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_345_133 56 (16.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_345_133 37 (24.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_345_133 32 (21.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_345_133 44 (17.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_345_133 41 (12.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_345_133 32 (21.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_345_133 56 (16.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_345_133 37 (16.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_106_131 43 (32.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_106_131 36 (33.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_106_131 61 (33.5) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_106_131 42 (21.0) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_106_131 36 (33.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_106_131 48 (21.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_106_131 36 (33.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_106_131 61 (33.5) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_106_131 64 (31.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_106_131 36 (33.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_106_131 43 (33.5) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_106_131 35 (40.5) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_106_131 31 (26.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_106_131 32 (11.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_106_131 31 (32.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_106_131 32 (11.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_106_131 52 (24.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_106_131 37 (24.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_106_131 32 (32.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_106_131 36 (26.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_106_131 32 (7.5) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_106_131 31 (32.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_106_131 32 (11.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_106_131 52 (24.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_106_131 36 (29.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_106_131 32 (11.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_106_131 34 (24.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_106_131 32 (32.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_106_131 32 (11.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_106_131 52 (24.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_106_131 37 (24.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_916_471 37 (25.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_916_471 52 (12.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_916_471 73 (18.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_916_471 43 (20.0) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_916_471 52 (12.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_916_471 35 (32.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_916_471 52 (12.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_916_471 73 (18.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_916_471 70 (20.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_916_471 52 (12.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_916_471 49 (17.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_916_471 31 (35.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_916_471 34 (18.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_916_471 30 (20.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_916_471 40 (21.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_916_471 30 (20.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_916_471 46 (24.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_916_471 38 (19.0) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_916_471 31 (28.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_916_471 37 (18.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_916_471 32 (20.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_916_471 40 (21.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_916_471 30 (20.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_916_471 46 (24.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_916_471 37 (15.0) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_916_471 30 (20.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_916_471 37 (13.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_916_471 40 (17.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_916_471 30 (20.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_916_471 46 (24.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_916_471 38 (19.0) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_57_85 39 (17.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_57_85 45 (13.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_57_85 66 (15.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_57_85 45 (29.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_57_85 45 (13.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_57_85 35 (41.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_57_85 45 (13.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_57_85 66 (15.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_57_85 72 (29.5) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_57_85 45 (13.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_57_85 50 (15.5) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_57_85 40 (20.5) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_57_85 38 (11.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_57_85 32 (29.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_57_85 29 (7.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_57_85 32 (29.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_57_85 51 (18.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_57_85 45 (12.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_57_85 28 (18.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_57_85 38 (16.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_57_85 34 (29.5) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_57_85 29 (7.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_57_85 32 (29.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_57_85 51 (18.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_57_85 38 (22.0) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_57_85 32 (29.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_57_85 38 (12.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_57_85 30 (7.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_57_85 32 (29.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_57_85 51 (18.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_57_85 45 (12.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_10_437 50 (31.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_10_437 45 (18.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_10_437 70 (18.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_10_437 49 (23.0) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_10_437 45 (18.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_10_437 36 (29.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_10_437 45 (18.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_10_437 70 (18.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_10_437 76 (23.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_10_437 45 (18.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_10_437 48 (20.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_10_437 37 (13.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_10_437 44 (15.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_10_437 36 (23.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_10_437 38 (31.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_10_437 36 (23.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_10_437 51 (17.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_10_437 48 (16.0) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_10_437 35 (14.5) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_10_437 47 (15.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_10_437 38 (23.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_10_437 38 (31.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_10_437 36 (23.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_10_437 51 (17.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_10_437 47 (19.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_10_437 36 (23.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_10_437 43 (10.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_10_437 38 (27.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_10_437 36 (23.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_10_437 51 (17.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_10_437 48 (16.0) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_154_436 40 (22.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_154_436 38 (33.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_154_436 63 (33.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_154_436 41 (26.0) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_154_436 38 (33.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_154_436 37 (22.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_154_436 38 (33.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_154_436 63 (33.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_154_436 65 (36.5) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_154_436 38 (33.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_154_436 43 (18.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_154_436 31 (42.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_154_436 29 (25.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_154_436 34 (29.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_154_436 33 (16.5) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_154_436 34 (29.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_154_436 58 (27.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_154_436 34 (21.0) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_154_436 31 (35.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_154_436 31 (21.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_154_436 34 (25.5) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_154_436 33 (16.5) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_154_436 34 (29.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_154_436 58 (27.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_154_436 34 (21.0) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_154_436 34 (29.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_154_436 32 (19.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_154_436 34 (16.5) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_154_436 34 (29.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_154_436 58 (27.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_154_436 34 (21.0) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_336_64 44 (29.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_336_64 38 (22.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_336_64 61 (19.5) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_336_64 39 (34.0) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_336_64 38 (22.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_336_64 35 (35.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_336_64 38 (22.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_336_64 61 (19.5) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_336_64 66 (26.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_336_64 38 (22.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_336_64 46 (18.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_336_64 40 (16.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_336_64 33 (29.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_336_64 28 (23.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_336_64 36 (18.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_336_64 28 (23.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_336_64 53 (30.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_336_64 39 (29.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_336_64 35 (10.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_336_64 36 (29.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_336_64 30 (23.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_336_64 36 (18.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_336_64 28 (23.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_336_64 53 (30.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_336_64 39 (29.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_336_64 28 (23.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_336_64 35 (20.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_336_64 37 (18.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_336_64 28 (23.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_336_64 53 (30.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_336_64 39 (29.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_620_233 50 (22.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_620_233 40 (20.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_620_233 61 (28.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_620_233 50 (16.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_620_233 40 (20.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_620_233 44 (15.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_620_233 40 (20.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_620_233 61 (28.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_620_233 67 (33.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_620_233 40 (20.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_620_233 43 (33.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_620_233 34 (27.5) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_620_233 38 (26.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_620_233 34 (6.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_620_233 38 (22.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_620_233 34 (6.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_620_233 57 (21.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_620_233 40 (27.0) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_620_233 34 (20.5) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_620_233 39 (26.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_620_233 36 (6.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_620_233 38 (22.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_620_233 34 (6.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_620_233 57 (21.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_620_233 42 (26.0) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_620_233 34 (6.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_620_233 34 (27.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_620_233 38 (27.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_620_233 34 (6.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_620_233 57 (21.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_620_233 40 (27.0) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_902_349 43 (25.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_902_349 42 (11.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_902_349 68 (32.5) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_902_349 40 (16.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_902_349 42 (11.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_902_349 46 (28.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_902_349 42 (11.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_902_349 68 (32.5) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_902_349 64 (22.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_902_349 42 (11.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_902_349 53 (18.5) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_902_349 34 (25.5) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_902_349 32 (16.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_902_349 35 (16.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_902_349 34 (13.5) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_902_349 35 (16.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_902_349 54 (32.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_902_349 39 (20.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_902_349 41 (25.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_902_349 35 (16.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_902_349 37 (16.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_902_349 34 (13.5) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_902_349 35 (16.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_902_349 54 (32.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_902_349 38 (16.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_902_349 35 (16.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_902_349 34 (16.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_902_349 35 (13.5) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_902_349 35 (16.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_902_349 54 (32.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_902_349 39 (20.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_40_618 44 (10.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_40_618 40 (30.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_40_618 65 (30.5) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_40_618 57 (10.0) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_40_618 40 (30.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_40_618 36 (12.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_40_618 40 (30.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_40_618 65 (30.5) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_40_618 72 (16.5) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_40_618 40 (30.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_40_618 48 (16.5) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_40_618 45 (12.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_40_618 42 (17.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_40_618 38 (5.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_40_618 32 (10.5) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_40_618 38 (5.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_40_618 61 (13.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_40_618 50 (13.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_40_618 40 (12.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_40_618 43 (18.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_40_618 44 (2.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_40_618 32 (10.5) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_40_618 38 (5.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_40_618 61 (13.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_40_618 46 (18.0) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_40_618 38 (5.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_40_618 44 (13.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_40_618 43 (11.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_40_618 38 (5.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_40_618 61 (13.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_40_618 50 (13.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_83_511 35 (31.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_83_511 42 (28.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_83_511 64 (42.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_83_511 42 (22.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_83_511 42 (28.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_83_511 48 (12.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_83_511 42 (28.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_83_511 64 (42.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_83_511 69 (25.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_83_511 42 (28.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_83_511 45 (25.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_83_511 37 (30.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_83_511 34 (28.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_83_511 36 (12.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_83_511 30 (23.5) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_83_511 36 (12.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_83_511 53 (23.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_83_511 39 (22.0) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_83_511 32 (30.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_83_511 34 (25.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_83_511 38 (12.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_83_511 30 (23.5) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_83_511 36 (12.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_83_511 53 (23.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_83_511 37 (25.0) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_83_511 36 (12.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_83_511 33 (22.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_83_511 31 (23.5) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_83_511 36 (12.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_83_511 53 (23.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_83_511 39 (22.0) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_76_246 47 (21.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_76_246 50 (18.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_76_246 75 (18.5) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_76_246 51 (18.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_76_246 50 (18.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_76_246 36 (25.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_76_246 50 (18.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_76_246 75 (18.5) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_76_246 65 (29.5) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_76_246 50 (18.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_76_246 48 (21.5) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_76_246 39 (20.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_76_246 36 (23.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_76_246 38 (13.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_76_246 38 (17.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_76_246 38 (13.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_76_246 54 (23.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_76_246 42 (23.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_76_246 28 (14.5) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_76_246 39 (23.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_76_246 40 (13.5) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_76_246 38 (17.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_76_246 38 (13.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_76_246 54 (23.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_76_246 42 (23.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_76_246 38 (13.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_76_246 36 (23.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_76_246 30 (14.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_76_246 38 (13.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_76_246 54 (23.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_76_246 42 (23.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_303_427 35 (29.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_303_427 47 (31.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_303_427 59 (35.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_303_427 45 (27.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_303_427 47 (31.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_303_427 46 (14.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_303_427 47 (31.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_303_427 59 (35.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_303_427 65 (25.5) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_303_427 47 (31.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_303_427 41 (25.5) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_303_427 29 (17.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_303_427 33 (40.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_303_427 40 (12.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_303_427 30 (22.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_303_427 40 (12.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_303_427 51 (40.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_303_427 38 (44.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_303_427 28 (16.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_303_427 36 (40.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_303_427 40 (8.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_303_427 30 (22.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_303_427 40 (12.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_303_427 51 (40.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_303_427 39 (40.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_303_427 40 (12.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_303_427 33 (40.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_303_427 30 (27.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_303_427 40 (12.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_303_427 51 (40.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_303_427 38 (44.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_31_299 39 (26.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_31_299 36 (34.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_31_299 60 (37.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_31_299 44 (27.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_31_299 36 (34.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_31_299 39 (16.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_31_299 36 (34.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_31_299 60 (37.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_31_299 66 (32.5) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_31_299 36 (34.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_31_299 42 (32.5) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_31_299 44 (17.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_31_299 35 (30.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_31_299 31 (27.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_31_299 38 (22.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_31_299 31 (27.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_31_299 54 (26.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_31_299 39 (29.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_31_299 39 (17.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_31_299 35 (28.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_31_299 33 (27.5) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_31_299 38 (22.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_31_299 31 (27.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_31_299 54 (26.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_31_299 38 (28.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_31_299 31 (27.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_31_299 33 (29.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_31_299 41 (19.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_31_299 31 (27.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_31_299 54 (26.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_31_299 39 (29.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_553_135 45 (15.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_553_135 49 (9.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_553_135 61 (25.5) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_553_135 37 (20.0) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_553_135 49 (9.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_553_135 33 (44.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_553_135 49 (9.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_553_135 61 (25.5) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_553_135 62 (22.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_553_135 49 (9.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_553_135 47 (14.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_553_135 34 (17.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_553_135 42 (14.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_553_135 33 (18.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_553_135 33 (6.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_553_135 33 (18.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_553_135 44 (28.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_553_135 44 (16.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_553_135 38 (10.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_553_135 41 (13.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_553_135 35 (18.5) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_553_135 33 (6.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_553_135 33 (18.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_553_135 44 (28.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_553_135 44 (13.0) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_553_135 33 (18.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_553_135 38 (16.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_553_135 44 (6.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_553_135 33 (18.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_553_135 44 (28.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_553_135 44 (16.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_139_74 41 (19.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_139_74 41 (22.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_139_74 58 (26.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_139_74 46 (21.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_139_74 41 (22.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_139_74 44 (20.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_139_74 41 (22.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_139_74 58 (26.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_139_74 66 (25.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_139_74 41 (22.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_139_74 42 (25.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_139_74 42 (16.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_139_74 36 (34.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_139_74 39 (18.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_139_74 35 (11.5) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_139_74 39 (18.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_139_74 51 (46.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_139_74 38 (42.0) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_139_74 42 (9.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_139_74 39 (34.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_139_74 39 (14.5) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_139_74 35 (11.5) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_139_74 39 (18.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_139_74 51 (46.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_139_74 41 (38.0) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_139_74 39 (18.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_139_74 36 (32.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_139_74 36 (11.5) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_139_74 39 (18.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_139_74 51 (46.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_139_74 38 (42.0) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_108_33 41 (19.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_108_33 46 (2.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_108_33 67 (21.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_108_33 41 (13.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_108_33 46 (2.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_108_33 45 (23.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_108_33 46 (2.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_108_33 67 (21.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_108_33 64 (18.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_108_33 46 (2.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_108_33 40 (17.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_108_33 46 (16.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_108_33 44 (14.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_108_33 31 (10.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_108_33 29 (19.5) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_108_33 31 (10.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_108_33 44 (26.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_108_33 46 (12.0) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_108_33 43 (6.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_108_33 48 (21.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_108_33 31 (6.5) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_108_33 29 (19.5) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_108_33 31 (10.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_108_33 44 (26.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_108_33 51 (21.0) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_108_33 31 (10.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_108_33 40 (12.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_108_33 29 (15.5) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_108_33 31 (10.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_108_33 44 (26.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_108_33 46 (12.0) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_980_965 44 (30.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_980_965 38 (15.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_980_965 71 (15.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_980_965 48 (25.0) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_980_965 38 (15.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_980_965 36 (29.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_980_965 38 (15.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_980_965 71 (15.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_980_965 75 (25.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_980_965 38 (15.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_980_965 50 (12.5) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_980_965 45 (19.5) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_980_965 33 (37.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_980_965 35 (25.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_980_965 43 (15.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_980_965 35 (25.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_980_965 51 (37.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_980_965 39 (37.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_980_965 30 (16.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_980_965 36 (37.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_980_965 37 (25.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_980_965 43 (15.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_980_965 35 (25.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_980_965 51 (37.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_980_965 39 (37.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_980_965 35 (25.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_980_965 35 (29.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_980_965 44 (15.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_980_965 35 (25.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_980_965 51 (37.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_980_965 39 (37.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_981_857 35 (27.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_981_857 41 (26.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_981_857 66 (26.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_981_857 39 (21.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_981_857 41 (26.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_981_857 51 (19.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_981_857 41 (26.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_981_857 66 (26.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_981_857 63 (28.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_981_857 41 (26.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_981_857 47 (24.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_981_857 34 (25.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_981_857 34 (15.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_981_857 41 (16.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_981_857 30 (23.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_981_857 41 (16.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_981_857 51 (19.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_981_857 38 (15.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_981_857 34 (18.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_981_857 39 (20.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_981_857 41 (12.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_981_857 30 (23.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_981_857 41 (16.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_981_857 51 (19.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_981_857 41 (16.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_981_857 41 (16.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_981_857 39 (9.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_981_857 30 (19.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_981_857 41 (16.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_981_857 51 (19.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_981_857 38 (15.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_8_865 49 (14.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_8_865 39 (35.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_8_865 64 (35.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_8_865 45 (20.0) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_8_865 39 (35.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_8_865 40 (27.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_8_865 39 (35.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_8_865 64 (35.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_8_865 72 (20.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_8_865 39 (35.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_8_865 45 (15.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_8_865 40 (19.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_8_865 43 (18.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_8_865 32 (20.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_8_865 34 (14.5) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_8_865 32 (20.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_8_865 44 (20.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_8_865 39 (16.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_8_865 40 (21.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_8_865 38 (15.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_8_865 34 (20.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_8_865 34 (14.5) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_8_865 32 (20.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_8_865 44 (20.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_8_865 38 (19.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_8_865 32 (20.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_8_865 42 (12.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_8_865 34 (10.5) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_8_865 32 (20.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_8_865 44 (20.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_8_865 39 (16.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_292_484 37 (16.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_292_484 35 (22.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_292_484 58 (22.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_292_484 36 (24.0) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_292_484 35 (22.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_292_484 40 (31.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_292_484 35 (22.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_292_484 58 (22.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_292_484 61 (34.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_292_484 35 (22.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_292_484 37 (34.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_292_484 34 (16.5) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_292_484 33 (22.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_292_484 35 (10.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_292_484 30 (14.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_292_484 35 (10.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_292_484 53 (19.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_292_484 37 (22.0) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_292_484 29 (16.5) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_292_484 44 (19.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_292_484 35 (6.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_292_484 30 (14.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_292_484 35 (10.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_292_484 53 (19.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_292_484 47 (19.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_292_484 35 (10.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_292_484 31 (22.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_292_484 38 (16.5) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_292_484 35 (10.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_292_484 53 (19.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_292_484 37 (22.0) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_675_16 39 (17.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_675_16 56 (10.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_675_16 81 (10.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_675_16 39 (16.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_675_16 56 (10.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_675_16 43 (32.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_675_16 56 (10.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_675_16 81 (10.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_675_16 67 (26.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_675_16 56 (10.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_675_16 43 (26.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_675_16 43 (13.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_675_16 40 (17.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_675_16 33 (13.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_675_16 42 (11.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_675_16 33 (13.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_675_16 51 (17.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_675_16 41 (22.0) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_675_16 38 (13.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_675_16 43 (17.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_675_16 35 (13.5) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_675_16 42 (11.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_675_16 33 (13.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_675_16 51 (17.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_675_16 43 (21.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_675_16 33 (13.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_675_16 48 (15.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_675_16 42 (7.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_675_16 33 (13.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_675_16 51 (17.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_675_16 41 (22.0) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_285_136 40 (21.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_285_136 45 (17.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_285_136 65 (30.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_285_136 39 (16.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_285_136 45 (17.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_285_136 52 (7.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_285_136 45 (17.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_285_136 65 (30.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_285_136 61 (21.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_285_136 45 (17.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_285_136 48 (18.5) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_285_136 32 (27.5) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_285_136 31 (13.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_285_136 43 (6.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_285_136 35 (17.5) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_285_136 43 (6.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_285_136 46 (12.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_285_136 36 (17.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_285_136 31 (26.5) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_285_136 32 (14.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_285_136 43 (2.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_285_136 35 (17.5) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_285_136 43 (6.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_285_136 46 (12.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_285_136 33 (15.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_285_136 43 (6.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_285_136 44 (15.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_285_136 36 (17.5) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_285_136 43 (6.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_285_136 46 (12.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_285_136 36 (17.5) + +#--------------------------------------- +#---------------------------------------