diff --git a/tools/busco/busco.xml b/tools/busco/busco.xml
index 3de694f1fd4..b9c51162d94 100644
--- a/tools/busco/busco.xml
+++ b/tools/busco/busco.xml
@@ -1,5 +1,5 @@
-<tool id="busco" name="Busco" profile="20.01" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@">
-    <description>assess genome assembly and annotation completeness</description>
+<tool id="busco" name="Busco" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="20.01">
+    <description>Assess genome assembly and annotation completeness</description>
     <macros>
         <import>macros.xml</import>
     </macros>
@@ -54,7 +54,6 @@ busco
     #elif $busco_mode.use_augustus.use_augustus_selector == 'augustus':
         ${busco_mode.use_augustus.long}
         --augustus
-
         #if $busco_mode.use_augustus.aug_prediction.augustus_mode == 'builtin':
             --augustus_species '${busco_mode.use_augustus.aug_prediction.augustus_species}'
         #else if $busco_mode.use_augustus.aug_prediction.augustus_mode == 'history':
@@ -84,9 +83,9 @@ busco
 #end if
 
 
-]]>    </command>
+]]></command>
     <inputs>
-        <param type="data" name="input" format="fasta" label="Sequences to analyse" help="Can be an assembled genome or transcriptome (DNA), or protein sequences from an annotated gene set." />
+        <param type="data" name="input" format="fasta" label="Sequences to analyse" help="Can be an assembled genome or transcriptome (DNA), or protein sequences from an annotated gene set."/>
         <conditional name="lineage_conditional">
             <param name="selector" type="select" label="Lineage data source">
                 <option value="download">Download lineage data</option>
@@ -95,7 +94,7 @@ busco
             <when value="cached">
                 <param name="cached_db" label="Cached database with lineage" type="select">
                     <options from_data_table="busco_database">
-                        <validator message="No BUSCO database is available" type="no_options" />
+                        <validator message="No BUSCO database is available" type="no_options"/>
                     </options>
                 </param>
             </when>
@@ -113,7 +112,6 @@ busco
                         <option value="augustus">Augustus</option>
                         <option value="metaeuk">Metaeuk</option>
                         <option value="miniprot" selected="true">Miniprot</option>
-
                     </param>
                     <when value="metaeuk"/>
                     <when value="miniprot"/>
@@ -124,24 +122,23 @@ busco
                                 <option value="builtin">Use another predefined species model</option>
                                 <option value="history">Use a custom species model</option>
                             </param>
-                            <when value="no" />
+                            <when value="no"/>
                             <when value="history">
-                                <param name="augustus_model" type="data" format="augustus" label="Augustus model" />
+                                <param name="augustus_model" type="data" format="augustus" label="Augustus model"/>
                             </when>
                             <when value="builtin">
                                 <param name="augustus_species" type="select" label="Augustus species model">
-                                    <expand macro="augustus_species" />
+                                    <expand macro="augustus_species"/>
                                 </param>
                             </when>
                         </conditional>
-                        <param argument="--long" type="boolean" checked="false" truevalue="--long" falsevalue="" label="Optimization mode Augustus self-training" help="Adds considerably to run time, but can improve results for some non-model organisms" />
+                        <param argument="--long" type="boolean" checked="false" truevalue="--long" falsevalue="" label="Optimization mode Augustus self-training" help="Adds considerably to run time, but can improve results for some non-model organisms"/>
                     </when>
                 </conditional>
             </when>
-            <when value="tran" />
-            <when value="prot" />
+            <when value="tran"/>
+            <when value="prot"/>
         </conditional>
-
         <conditional name="lineage">
             <param name="lineage_mode" type="select" label="Auto-detect or select lineage?" help="Let BUSCO decide the best lineage automatically, or select from known lineage">
                 <option value="auto_detect">Auto-detect</option>
@@ -156,7 +153,7 @@ busco
             </when>
             <when value="select_lineage">
                 <param argument="--lineage_dataset" type="select" label="Lineage">
-                    <expand macro="lineages" />
+                    <expand macro="lineages"/>
                 </param>
             </when>
         </conditional>
@@ -169,8 +166,8 @@ busco
         </param>
 
         <section name="adv" title="Advanced Options" expanded="False">
-            <param argument="--evalue" type="float" value="0.001" min="0" max="1" label="E-value cutoff for BLAST searches." />
-            <param argument="--limit" type="integer" value="3" label="How many candidate regions to consider" />
+            <param argument="--evalue" type="float" value="0.001" min="0" max="1" label="E-value cutoff for BLAST searches."/>
+            <param argument="--limit" type="integer" value="3" label="How many candidate regions to consider"/>
             <param argument="--contig_break" type="integer" value="10" label="Number of contiguous Ns to signify a break between contigs"/>
         </section>
     </inputs>
@@ -179,7 +176,7 @@ busco
         <data name='busco_sum' format='txt' label="${tool.name} on ${on_string}: short summary" from_work_dir="busco_galaxy/run_*/short_summary.txt">
             <filter>outputs and 'short_summary' in outputs</filter>
         </data>
-        <data name='busco_table' format='tabular' label="${tool.name} on ${on_string}: full table" from_work_dir="busco_galaxy/run_*/full_table.tsv" />
+        <data name='busco_table' format='tabular' label="${tool.name} on ${on_string}: full table" from_work_dir="busco_galaxy/run_*/full_table.tsv"/>
         <data name='busco_missing' format='tabular' label="${tool.name} on ${on_string}: missing buscos" from_work_dir="busco_galaxy/run_*/missing_busco_list.tsv">
             <filter>outputs and 'missing' in outputs</filter>
         </data>
@@ -193,148 +190,148 @@ busco
     
     <tests>
         <test expect_num_outputs="4">
-            <param name="input" value="genome.fa" />
+            <param name="input" value="genome.fa"/>
             <conditional name="lineage">
-                <param name="lineage_mode" value="select_lineage" />
-                <param name="lineage_dataset" value="arthropoda_odb10" />
+                <param name="lineage_mode" value="select_lineage"/>
+                <param name="lineage_dataset" value="arthropoda_odb10"/>
             </conditional>
             <conditional name="busco_mode">
-                <param name="mode" value="geno" />
+                <param name="mode" value="geno"/>
                 <conditional name="use_augustus">
-                    <param name="use_augustus_selector" value="augustus" />
+                    <param name="use_augustus_selector" value="augustus"/>
                 </conditional>
             </conditional>
-            <param name="outputs" value="short_summary,missing,gff" />
+            <param name="outputs" value="short_summary,missing,gff"/>
             <output name="busco_sum">
                 <assert_contents>
                     <has_text text="# BUSCO version is: @TOOL_VERSION@"/>
                     <has_text text="Gene predictor used: augustus"/>
                 </assert_contents>
             </output>
-            <output name="busco_table" file="genome_results/full_table" compare="diff" lines_diff="10" />
+            <output name="busco_table" file="genome_results/full_table" compare="diff" lines_diff="10"/>
             <output name="busco_gff">
                 <assert_contents>
                     <has_n_lines n="1"/>
-                    <has_text text="##gff-version 3" />
+                    <has_text text="##gff-version 3"/>
                 </assert_contents>
             </output>
             <output name="busco_missing" file="genome_results/missing_buscos_list" compare="diff" lines_diff="10"/>
         </test>
         <test expect_num_outputs="5">
-            <param name="input" value="proteome.fa" />
+            <param name="input" value="proteome.fa"/>
             <conditional name="lineage">
-                <param name="lineage_mode" value="select_lineage" />
-                <param name="lineage_dataset" value="arthropoda_odb10" />
+                <param name="lineage_mode" value="select_lineage"/>
+                <param name="lineage_dataset" value="arthropoda_odb10"/>
             </conditional>
             <conditional name="busco_mode">
-                <param name="mode" value="prot" />
+                <param name="mode" value="prot"/>
             </conditional>
-            <param name="outputs" value="short_summary,missing,image,gff" />
+            <param name="outputs" value="short_summary,missing,image,gff"/>
             <output name="busco_sum">
                 <assert_contents>
                     <has_text text="# BUSCO version is: @TOOL_VERSION@"/>
-                    <has_text text="BUSCO was run in mode: proteins" />
+                    <has_text text="BUSCO was run in mode: proteins"/>
                 </assert_contents>
             </output>
-            <output name="busco_table" file="proteome_results/full_table" compare="diff" lines_diff="10" />
-            <output name="busco_missing" file="proteome_results/missing_buscos_list" compare="diff" lines_diff="10" />
-            <output name="summary_image" file="proteome_results/summary.png" compare="sim_size" />
+            <output name="busco_table" file="proteome_results/full_table" compare="diff" lines_diff="10"/>
+            <output name="busco_missing" file="proteome_results/missing_buscos_list" compare="diff" lines_diff="10"/>
+            <output name="summary_image" file="proteome_results/summary.png" compare="sim_size"/>
             <output name="busco_gff">
                 <assert_contents>
                     <has_n_lines n="1"/>
-                    <has_text text="##gff-version 3" />
+                    <has_text text="##gff-version 3"/>
                 </assert_contents>
             </output>
         </test>
         <test expect_num_outputs="5">
-            <param name="input" value="transcriptome.fa" />
+            <param name="input" value="transcriptome.fa"/>
             <conditional name="lineage">
-                <param name="lineage_mode" value="select_lineage" />
-                <param name="lineage_dataset" value="arthropoda_odb10" />
+                <param name="lineage_mode" value="select_lineage"/>
+                <param name="lineage_dataset" value="arthropoda_odb10"/>
             </conditional>
             <conditional name="busco_mode">
-                <param name="mode" value="tran" />
+                <param name="mode" value="tran"/>
             </conditional>
-            <param name="outputs" value="short_summary,missing,image,gff" />
+            <param name="outputs" value="short_summary,missing,image,gff"/>
             <output name="busco_sum">
                 <assert_contents>
-                    <has_text text="BUSCO was run in mode: euk_tran" />
+                    <has_text text="BUSCO was run in mode: euk_tran"/>
                 </assert_contents>
             </output>
-            <output name="busco_table" file="transcriptome_results/full_table" compare="diff" lines_diff="6" />
-            <output name="busco_missing" file="transcriptome_results/missing_buscos_list" compare="diff" lines_diff="6" />
-            <output name="summary_image" file="transcriptome_results/summary.png" compare="sim_size" />
-            <output name="busco_gff" file="transcriptome_results/out.gff" compare="diff" />
+            <output name="busco_table" file="transcriptome_results/full_table" compare="diff" lines_diff="6"/>
+            <output name="busco_missing" file="transcriptome_results/missing_buscos_list" compare="diff" lines_diff="6"/>
+            <output name="summary_image" file="transcriptome_results/summary.png" compare="sim_size"/>
+            <output name="busco_gff" file="transcriptome_results/out.gff" compare="diff"/>
         </test>
         <test expect_num_outputs="3">
-            <param name="input" value="genome.fa" />
+            <param name="input" value="genome.fa"/>
             <conditional name="lineage">
-                <param name="lineage_mode" value="select_lineage" />
-                <param name="lineage_dataset" value="arthropoda_odb10" />
+                <param name="lineage_mode" value="select_lineage"/>
+                <param name="lineage_dataset" value="arthropoda_odb10"/>
             </conditional>
             <conditional name="busco_mode">
-                <param name="mode" value="geno" />
+                <param name="mode" value="geno"/>
                 <conditional name="use_augustus">
-                    <param name="use_augustus_selector" value="augustus" />
+                    <param name="use_augustus_selector" value="augustus"/>
                     <conditional name="aug_prediction">
-                        <param name="augustus_mode" value="builtin" />
-                        <param name="augustus_species" value="human" />
+                        <param name="augustus_mode" value="builtin"/>
+                        <param name="augustus_species" value="human"/>
                     </conditional>
                 </conditional>
             </conditional>
-            <param name="outputs" value="short_summary,gff" />
+            <param name="outputs" value="short_summary,gff"/>
             <output name="busco_sum" file="genome_results/short_summary" compare="diff" lines_diff="10">
                 <assert_contents>
                     <has_text text="# Gene predictor used: augustus"/>
                 </assert_contents>
             </output>
-            <output name="busco_table" file="genome_results/full_table" compare="diff" lines_diff="10" />
+            <output name="busco_table" file="genome_results/full_table" compare="diff" lines_diff="10"/>
             <output name="busco_gff">
                 <assert_contents>
                     <has_n_lines n="1"/>
-                    <has_text text="##gff-version 3" />
+                    <has_text text="##gff-version 3"/>
                 </assert_contents>
             </output>
         </test>
         <test expect_num_outputs="3">
-            <param name="input" value="genome.fa" />
+            <param name="input" value="genome.fa"/>
             <conditional name="lineage">
-                <param name="lineage_mode" value="select_lineage" />
-                <param name="lineage_dataset" value="arthropoda_odb10" />
+                <param name="lineage_mode" value="select_lineage"/>
+                <param name="lineage_dataset" value="arthropoda_odb10"/>
             </conditional>
             <conditional name="busco_mode">
-                <param name="mode" value="geno" />
+                <param name="mode" value="geno"/>
                 <conditional name="use_augustus">
-                    <param name="use_augustus_selector" value="augustus" />
+                    <param name="use_augustus_selector" value="augustus"/>
                     <conditional name="aug_prediction">
-                        <param name="augustus_mode" value="history" />
-                        <param name="augustus_model" value="local.tar.gz" ftype="augustus" />
+                        <param name="augustus_mode" value="history"/>
+                        <param name="augustus_model" value="local.tar.gz" ftype="augustus"/>
                     </conditional>
                 </conditional>
             </conditional>
-            <param name="outputs" value="short_summary,missing" />
+            <param name="outputs" value="short_summary,missing"/>
             <output name="busco_sum" file="genome_results/short_summary" compare="diff" lines_diff="10">
                 <assert_contents>
                     <has_text text="# Gene predictor used: augustus"/>
                     <has_text text="# BUSCO version is: @TOOL_VERSION@"/>
                 </assert_contents>
             </output>
-            <output name="busco_table" file="genome_results/full_table" compare="diff" lines_diff="10" />
-            <output name="busco_missing" file="genome_results/missing_buscos_list" compare="diff" lines_diff="10" />
+            <output name="busco_table" file="genome_results/full_table" compare="diff" lines_diff="10"/>
+            <output name="busco_missing" file="genome_results/missing_buscos_list" compare="diff" lines_diff="10"/>
         </test>
         <test expect_num_outputs="5">
-            <param name="input" value="genome.fa" />
+            <param name="input" value="genome.fa"/>
             <conditional name="lineage">
-                <param name="lineage_mode" value="select_lineage" />
-                <param name="lineage_dataset" value="arthropoda_odb10" />
+                <param name="lineage_mode" value="select_lineage"/>
+                <param name="lineage_dataset" value="arthropoda_odb10"/>
             </conditional>
             <conditional name="busco_mode">
-                <param name="mode" value="geno" />
+                <param name="mode" value="geno"/>
                 <conditional name="use_augustus">
-                    <param name="use_augustus_selector" value="metaeuk" />
+                    <param name="use_augustus_selector" value="metaeuk"/>
                 </conditional>
             </conditional>
-            <param name="outputs" value="short_summary,missing,image,gff" />
+            <param name="outputs" value="short_summary,missing,image,gff"/>
             <output name="busco_sum">
                 <assert_contents>
                     <has_text text="# Gene predictor used: metaeuk"/>
@@ -353,21 +350,21 @@ busco
                 </assert_contents>
             </output>
             <output name="summary_image" file="genome_results_metaeuk/summary.png" compare="sim_size"/>
-            <output name="busco_gff" file="genome_results_metaeuk/out.gff3" compare="diff" lines_diff="6" />
+            <output name="busco_gff" file="genome_results_metaeuk/out.gff3" compare="diff" lines_diff="6"/>
         </test>
         <test expect_num_outputs="5">
-            <param name="input" value="genome.fa" />
+            <param name="input" value="genome.fa"/>
             <conditional name="lineage">
-                <param name="lineage_mode" value="auto_detect" />
-                <param name="auto_lineage" value="--auto-lineage" />
+                <param name="lineage_mode" value="auto_detect"/>
+                <param name="auto_lineage" value="--auto-lineage"/>
             </conditional>
             <conditional name="busco_mode">
-                <param name="mode" value="geno" />
+                <param name="mode" value="geno"/>
                 <conditional name="use_augustus">
-                    <param name="use_augustus_selector" value="metaeuk" />
+                    <param name="use_augustus_selector" value="metaeuk"/>
                 </conditional>
             </conditional>
-            <param name="outputs" value="short_summary,missing,image,gff" />
+            <param name="outputs" value="short_summary,missing,image,gff"/>
             <output name="busco_sum">
                 <assert_contents>
                     <has_text text="Gene predictor used: metaeuk"/>
@@ -385,22 +382,22 @@ busco
                     <has_text text="The lineage dataset is: eukaryota_odb10"/>
                 </assert_contents>
             </output>
-            <output name="summary_image" file="genome_results_metaeuk_auto/summary.png" compare="sim_size" />
-            <output name="busco_gff" file="genome_results_metaeuk_auto/out.gff" compare="diff" />
+            <output name="summary_image" file="genome_results_metaeuk_auto/summary.png" compare="sim_size"/>
+            <output name="busco_gff" file="genome_results_metaeuk_auto/out.gff" compare="diff"/>
         </test>
         <test expect_num_outputs="3">
-            <param name="input" value="genome.fa" />
+            <param name="input" value="genome.fa"/>
             <conditional name="lineage">
-                <param name="lineage_mode" value="select_lineage" />
-                <param name="lineage_dataset" value="arthropoda_odb10" />
+                <param name="lineage_mode" value="select_lineage"/>
+                <param name="lineage_dataset" value="arthropoda_odb10"/>
             </conditional>
-            <param name="outputs" value="short_summary,gff" />
+            <param name="outputs" value="short_summary,gff"/>
             <output name="busco_sum">
                 <assert_contents>
                     <has_text text="# Gene predictor used: miniprot"/>
                 </assert_contents>
             </output>
-            <output name="busco_gff" file="genome_results_miniprot/out.gff" compare="diff" />
+            <output name="busco_gff" file="genome_results_miniprot/out.gff" compare="diff"/>
             <output name="busco_table" file="genome_results_miniprot/full_table">
                 <assert_contents>
                     <has_text text="# BUSCO version is: @TOOL_VERSION@"/>
@@ -412,7 +409,7 @@ busco
     <help><![CDATA[
 
 
-BUSCO: assessing genome assembly and annotation completeness with Benchmarking Universal Single-Copy Orthologs
+BUSCO: Assessing genome assembly and annotation completeness with Benchmarking Universal Single-Copy Orthologs
 --------------------------------------------------------------------------------------------------------------
 
 Interpreting the results
@@ -432,11 +429,11 @@ Finally, focusing on specific tissues or specific life stages and conditions in
 is unlikely to produce a BUSCO-complete transcriptome. In this case, consistency across your samples
 is what you will be aiming for.
 
-For more information please refer to the Busco_ `user guide <https: / /busco.ezlab.org /busco_userguide.html#interpreting-the-results>`_
+For more information please refer to the Busco_ `user guide <https://busco.ezlab.org /busco_userguide.html#interpreting-the-results>`_
 .
 
 .. _BUSCO: http://busco.ezlab.org/
 
-    ]]>    </help>
-    <expand macro="citations" />
+    ]]></help>
+    <expand macro="citations"/>
 </tool>
diff --git a/tools/busco/macros.xml b/tools/busco/macros.xml
index a62ca1b683a..9ba7d825fc9 100644
--- a/tools/busco/macros.xml
+++ b/tools/busco/macros.xml
@@ -1,6 +1,6 @@
 <?xml version="1.0"?>
 <macros>
-    <token name="@TOOL_VERSION@">5.7.1</token>
+    <token name="@TOOL_VERSION@">5.8.0</token>
     <token name="@VERSION_SUFFIX@">0</token>
 
     <xml name="citations">
diff --git a/tools/busco/test-data/genome_results/full_table b/tools/busco/test-data/genome_results/full_table
index 4df8b51f8f1..e22cea428ef 100644
--- a/tools/busco/test-data/genome_results/full_table
+++ b/tools/busco/test-data/genome_results/full_table
@@ -1,4 +1,4 @@
-# BUSCO version is: 5.7.1 
+# BUSCO version is: 5.8.0 
 # The lineage dataset is: arthropoda_odb10 (Creation date: 2024-01-08, number of genomes: 90, number of BUSCOs: 1013)
 # Busco id	Status	Sequence	Gene Start	Gene End	Strand	Score	Length	OrthoDB url	Description
 774at6656	Missing
@@ -509,7 +509,7 @@
 93535at6656	Missing
 93797at6656	Missing
 94054at6656	Missing
-94238at6656	Complete	sample	38486	34764	-	60.7	116	https://v10-1.orthodb.org/?query=94238at6656	Checkpoint protein HUS1
+94238at6656	Complete	sample	38486	34764	-	60.7	117	https://v10-1.orthodb.org/?query=94238at6656	Checkpoint protein HUS1
 94263at6656	Missing
 94304at6656	Missing
 94473at6656	Missing
diff --git a/tools/busco/test-data/genome_results/missing_buscos_list b/tools/busco/test-data/genome_results/missing_buscos_list
index 4814280ee53..b4ce48e8a0b 100644
--- a/tools/busco/test-data/genome_results/missing_buscos_list
+++ b/tools/busco/test-data/genome_results/missing_buscos_list
@@ -1,4 +1,4 @@
-# BUSCO version is: 5.7.1 
+# BUSCO version is: 5.8.0 
 # The lineage dataset is: arthropoda_odb10 (Creation date: 2024-01-08, number of genomes: 90, number of BUSCOs: 1013)
 # Busco id
 100070at6656
diff --git a/tools/busco/test-data/genome_results/short_summary b/tools/busco/test-data/genome_results/short_summary
index b0cc6f35564..2c28062fa0c 100644
--- a/tools/busco/test-data/genome_results/short_summary
+++ b/tools/busco/test-data/genome_results/short_summary
@@ -1,6 +1,6 @@
-# BUSCO version is: 5.7.1 
+# BUSCO version is: 5.8.0 
 # The lineage dataset is: arthropoda_odb10 (Creation date: 2024-01-08, number of genomes: 90, number of BUSCOs: 1013)
-# Summarized benchmarking in BUSCO notation for file /tmp/tmpl5l1blpe/files/7/a/3/dataset_7a33f452-1064-4b4a-943f-b0efef6a4a4a.dat
+# Summarized benchmarking in BUSCO notation for file /tmp/tmpmwpuw5w8/files/f/d/6/dataset_fd6995c7-d1df-43b9-b8be-7e62043550f0.dat
 # BUSCO was run in mode: euk_genome_aug
 # Gene predictor used: augustus
 
@@ -24,13 +24,13 @@ Assembly Statistics:
 
 
 Dependencies and versions:
-	hmmsearch: 3.1
-	bbtools: 39.06
-	makeblastdb: 2.15.0+
-	tblastn: 2.15.0+
+	hmmsearch: 3.4
+	bbtools: None
+	makeblastdb: 2.16.0+
+	tblastn: 2.16.0+
 	augustus: 3.5.0
 	gff2gbSmallDNA.pl: None
 	new_species.pl: None
 	etraining: None
-	python: sys.version_info(major=3, minor=9, micro=19, releaselevel='final', serial=0)
-	busco: 5.7.1
+	python: sys.version_info(major=3, minor=10, micro=14, releaselevel='final', serial=0)
+	busco: 5.8.0
diff --git a/tools/busco/test-data/genome_results_metaeuk/full_table b/tools/busco/test-data/genome_results_metaeuk/full_table
index 76071fd520d..bd707585d92 100644
--- a/tools/busco/test-data/genome_results_metaeuk/full_table
+++ b/tools/busco/test-data/genome_results_metaeuk/full_table
@@ -1,4 +1,4 @@
-# BUSCO version is: 5.7.1 
+# BUSCO version is: 5.8.0 
 # The lineage dataset is: arthropoda_odb10 (Creation date: 2024-01-08, number of genomes: 90, number of BUSCOs: 1013)
 # Busco id	Status	Sequence	Gene Start	Gene End	Strand	Score	Length	OrthoDB url	Description
 774at6656	Missing
@@ -340,7 +340,7 @@
 68939at6656	Missing
 68961at6656	Missing
 68981at6656	Missing
-68987at6656	Complete	sample	40255	42070	+	122.8	266	https://v10-1.orthodb.org/?query=68987at6656	Nucleotidyl transferase domain
+68987at6656	Complete	sample	40255	42070	+	122.8	267	https://v10-1.orthodb.org/?query=68987at6656	Nucleotidyl transferase domain
 69201at6656	Missing
 69238at6656	Missing
 69284at6656	Missing
@@ -509,7 +509,7 @@
 93535at6656	Missing
 93797at6656	Missing
 94054at6656	Missing
-94238at6656	Complete	sample	35693	34845	-	60.6	116	https://v10-1.orthodb.org/?query=94238at6656	Checkpoint protein HUS1
+94238at6656	Complete	sample	35693	34845	-	60.6	117	https://v10-1.orthodb.org/?query=94238at6656	Checkpoint protein HUS1
 94263at6656	Missing
 94304at6656	Missing
 94473at6656	Missing
diff --git a/tools/busco/test-data/genome_results_metaeuk/missing_buscos_list b/tools/busco/test-data/genome_results_metaeuk/missing_buscos_list
index df7cf8eb56a..58b593c065d 100644
--- a/tools/busco/test-data/genome_results_metaeuk/missing_buscos_list
+++ b/tools/busco/test-data/genome_results_metaeuk/missing_buscos_list
@@ -1,4 +1,4 @@
-# BUSCO version is: 5.7.1 
+# BUSCO version is: 5.8.0 
 # The lineage dataset is: arthropoda_odb10 (Creation date: 2024-01-08, number of genomes: 90, number of BUSCOs: 1013)
 # Busco id
 100070at6656
diff --git a/tools/busco/test-data/genome_results_metaeuk/summary.png b/tools/busco/test-data/genome_results_metaeuk/summary.png
index 19633353f1f..ef4d1bb9afb 100644
Binary files a/tools/busco/test-data/genome_results_metaeuk/summary.png and b/tools/busco/test-data/genome_results_metaeuk/summary.png differ
diff --git a/tools/busco/test-data/genome_results_metaeuk_auto/summary.png b/tools/busco/test-data/genome_results_metaeuk_auto/summary.png
index 208587fe399..bff44097c98 100644
Binary files a/tools/busco/test-data/genome_results_metaeuk_auto/summary.png and b/tools/busco/test-data/genome_results_metaeuk_auto/summary.png differ
diff --git a/tools/busco/test-data/genome_results_miniprot/full_table b/tools/busco/test-data/genome_results_miniprot/full_table
index 246512ee0ca..ee39157e509 100644
--- a/tools/busco/test-data/genome_results_miniprot/full_table
+++ b/tools/busco/test-data/genome_results_miniprot/full_table
@@ -1,4 +1,4 @@
-# BUSCO version is: 5.7.1 
+# BUSCO version is: 5.8.0 
 # The lineage dataset is: arthropoda_odb10 (Creation date: 2024-01-08, number of genomes: 90, number of BUSCOs: 1013)
 # Busco id	Status	Sequence	Gene Start	Gene End	Strand	Score	Length	OrthoDB url	Description
 774at6656	Missing
@@ -509,7 +509,7 @@
 93535at6656	Missing
 93797at6656	Missing
 94054at6656	Missing
-94238at6656	Complete	sample	35679	34845	-	60.7	116	https://v10-1.orthodb.org/?query=94238at6656	Checkpoint protein HUS1
+94238at6656	Complete	sample	35679	34845	-	60.7	117	https://v10-1.orthodb.org/?query=94238at6656	Checkpoint protein HUS1
 94263at6656	Missing
 94304at6656	Missing
 94473at6656	Missing
diff --git a/tools/busco/test-data/genome_results_miniprot/out.gff b/tools/busco/test-data/genome_results_miniprot/out.gff
index e69de29bb2d..3fabe5a07e4 100644
--- a/tools/busco/test-data/genome_results_miniprot/out.gff
+++ b/tools/busco/test-data/genome_results_miniprot/out.gff
@@ -0,0 +1,4 @@
+##gff-version 3
+sample	miniprot	mRNA	34843	35679	1341	-	.	ID=MP000459;Rank=1;Identity=0.9640;Positive=0.9856;Target=94238at6656_7220_0:000926 1 278
+sample	miniprot	CDS	34843	35679	1341	-	0	Parent=MP000459;Rank=1;Identity=0.9640;Target=94238at6656_7220_0:000926 1 278
+sample	miniprot	stop_codon	34843	34845	0	-	0	Parent=MP000459;Rank=1
diff --git a/tools/busco/test-data/proteome_results/full_table b/tools/busco/test-data/proteome_results/full_table
index 71851316127..36fa4550c0c 100644
--- a/tools/busco/test-data/proteome_results/full_table
+++ b/tools/busco/test-data/proteome_results/full_table
@@ -1,4 +1,4 @@
-# BUSCO version is: 5.7.1 
+# BUSCO version is: 5.8.0 
 # The lineage dataset is: arthropoda_odb10 (Creation date: 2024-01-08, number of genomes: 90, number of BUSCOs: 1013)
 # Busco id	Status	Sequence	Score	Length	OrthoDB url	Description
 774at6656	Missing
@@ -86,7 +86,7 @@
 22098at6656	Missing
 22148at6656	Missing
 22358at6656	Missing
-22364at6656	Complete	BUSCOaEOG7B0HST	1355.6	788	https://v10-1.orthodb.org/?query=22364at6656	Importin repeat 4
+22364at6656	Complete	BUSCOaEOG7B0HST	1355.6	789	https://v10-1.orthodb.org/?query=22364at6656	Importin repeat 4
 22468at6656	Missing
 22611at6656	Missing
 22887at6656	Missing
diff --git a/tools/busco/test-data/proteome_results/missing_buscos_list b/tools/busco/test-data/proteome_results/missing_buscos_list
index 2969d7bd270..91478d6ba9e 100644
--- a/tools/busco/test-data/proteome_results/missing_buscos_list
+++ b/tools/busco/test-data/proteome_results/missing_buscos_list
@@ -1,4 +1,4 @@
-# BUSCO version is: 5.7.1 
+# BUSCO version is: 5.8.0 
 # The lineage dataset is: arthropoda_odb10 (Creation date: 2024-01-08, number of genomes: 90, number of BUSCOs: 1013)
 # Busco id
 100070at6656
diff --git a/tools/busco/test-data/proteome_results/summary.png b/tools/busco/test-data/proteome_results/summary.png
index ac5b6fab951..d595b004077 100644
Binary files a/tools/busco/test-data/proteome_results/summary.png and b/tools/busco/test-data/proteome_results/summary.png differ
diff --git a/tools/busco/test-data/transcriptome_results/full_table b/tools/busco/test-data/transcriptome_results/full_table
index cc83d9fee82..cb93bc51ba4 100644
--- a/tools/busco/test-data/transcriptome_results/full_table
+++ b/tools/busco/test-data/transcriptome_results/full_table
@@ -1,4 +1,4 @@
-# BUSCO version is: 5.7.1 
+# BUSCO version is: 5.8.0 
 # The lineage dataset is: arthropoda_odb10 (Creation date: 2024-01-08, number of genomes: 90, number of BUSCOs: 1013)
 # Busco id	Status	Sequence	Score	Length	OrthoDB url	Description
 774at6656	Missing
@@ -86,7 +86,7 @@
 22098at6656	Missing
 22148at6656	Missing
 22358at6656	Missing
-22364at6656	Complete	BUSCOaEOG7B0HST:0-2699	1355.6	788	https://v10-1.orthodb.org/?query=22364at6656	Importin repeat 4
+22364at6656	Complete	BUSCOaEOG7B0HST:0-2699	1355.6	789	https://v10-1.orthodb.org/?query=22364at6656	Importin repeat 4
 22468at6656	Missing
 22611at6656	Missing
 22887at6656	Missing
diff --git a/tools/busco/test-data/transcriptome_results/missing_buscos_list b/tools/busco/test-data/transcriptome_results/missing_buscos_list
index 2969d7bd270..91478d6ba9e 100644
--- a/tools/busco/test-data/transcriptome_results/missing_buscos_list
+++ b/tools/busco/test-data/transcriptome_results/missing_buscos_list
@@ -1,4 +1,4 @@
-# BUSCO version is: 5.7.1 
+# BUSCO version is: 5.8.0 
 # The lineage dataset is: arthropoda_odb10 (Creation date: 2024-01-08, number of genomes: 90, number of BUSCOs: 1013)
 # Busco id
 100070at6656
diff --git a/tools/busco/test-data/transcriptome_results/summary.png b/tools/busco/test-data/transcriptome_results/summary.png
index ac5b6fab951..d595b004077 100644
Binary files a/tools/busco/test-data/transcriptome_results/summary.png and b/tools/busco/test-data/transcriptome_results/summary.png differ