diff --git a/communities/microgalaxy/lab/sections/1_data_import_and_preparation.yml b/communities/microgalaxy/lab/sections/1_data_import_and_preparation.yml
index 0c892cde..750b1593 100644
--- a/communities/microgalaxy/lab/sections/1_data_import_and_preparation.yml
+++ b/communities/microgalaxy/lab/sections/1_data_import_and_preparation.yml
@@ -1,15 +1,34 @@
id: data
-title: Data import and preparation
+title: Getting started
tabs:
- - id: tools
- title: Tools
+ - id: data-import
+ title: Data import
heading_md: >
- Common tools are listed here, or search for more in the full tool panel to the left.
+ Common tools that allow for data import.
content:
- title_md: Import data to Galaxy
description_md: >
Standard upload of data to Galaxy, from your computer or from the web.
button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=upload1"
+ - title_md: Download data from NCBI GenBank/RefSeq
+ description_md: >
+ Download sequences from GenBank/RefSeq by accession through the NCBI ENTREZ API
+ button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fncbi_acc_download%2Fncbi_acc_download"
+ - title_md: Download raw reads from NCBI SRA
+ description_md: >
+ Faster Download and Extract Reads in FASTQ format from NCBI SRA
+ button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsra_tools%2Ffasterq_dump"
+ - title_md: Download run data from EBI Metagenomics (MGnify)
+ description_md: >
+ This tool downloads data related to a run in EBI Metagenomics database
+ button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Febi_metagenomics_run_downloader%2Febi_metagenomics_run_downloader"
+
+
+ - id: highlight-tools
+ title: Highlight tools
+ heading_md: >
+ Flagship tools for microbial research
+ content:
- title_md: FastQC
- sequence quality reports
description_md: >
@@ -25,86 +44,48 @@ tabs:
- title_md: FastP
- sequence quality reports, trimming & filtering
description_md: >
- Faster run than FastQC, this tool can also trim reads and filter by quality. + Commonly used tool for trimming and quality filter. Also provides quality metrics.
inputs: - datatypes: - fastq button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Ffastp%2Ffastp" - - title_md:NanoPlot
- visualize Oxford Nanopore data
+ - title_md: Kraken2
- Kraken2 assign taxonomic labels to sequencing reads
description_md: >
- A plotting suite for Oxford Nanopore sequencing data and alignments. + Kraken2 assign taxonomic labels to sequencing reads.
inputs: - datatypes: - fastq - fasta - - vcf_bgzip - button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fnanoplot%2Fnanoplot" - - title_md:GenomeScope
- estimate genome size
- description_md: >
- - A set of metrics and graphs to visualize genome size and complexity prior to assembly. -
- inputs: - - datatypes: - - tabular - label: Output fromMeryl
or Jellyfish histo
- button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fgenomescope%2Fgenomescope"
- - title_md: Meryl
- count kmers
- description_md: >
- - Prepare kmer count histogram for input to GenomeScope. -
- inputs: - - datatypes: - - fastq - - fasta - button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fmeryl%2Fmeryl" - - - id: workflows - title: Workflows + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fkraken2%2Fkraken2" + + - id: learning-pathways + title: Learning pathways heading_md: > - A workflow is a series of Galaxy tools that have been linked together to perform a specific analysis. You can use and customize the example workflows below. - Learn more. + Connected tutorials to train you to perform microbial data analysis. content: - - title_md: Data QC + - title_md: Introduction to Galaxy and Sequence analysis description_md: > -
- Report statistics from sequencing reads.
Tools: nanoplot
fastqc
multiqc
-
- Estimates genome size and heterozygosity based on counts of kmers.
Tools: meryl
genomescope
-
- Trims and filters raw sequence reads according to specified settings.
Tools: fastp
-