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paramsElena.config
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// Extract all the parameters from the RNAseq.nf script and paste them in this separate params.config file.
// Define project parameters needed for running the pipeline
params {
// General parameters
//set output dir
outdir = "/user/gent/446/vsc44685/ScratchVO_dir/out_test_elena"
//Subsampling with SeqTk
Subs = 100
//Demux
M = 12 //length of the barcode
//path to save the pickle file (select the same as the barcode list)
bar_file = "/user/gent/446/vsc44685/ScratchVO_dir/ST_transcriptomics/ERV_barcodes"
P = "/user/gent/446/vsc44685/ScratchVO_dir/ST_transcriptomics/ERV_barcodes.pkl"
Ntriage = 2
//UMItools
UMItype = "semi" //could be semirandom or random
// Star
genome = "$VSC_DATA_VO/resources/STAR_index/Homo_sapiens/GRCh38.dna.primary_assembly.chrIS_spikes_45S.star_index"
threads = 10
gtf_file = "$VSC_DATA_VO/resources/Ensembl_transcriptomes/Homo_sapiens/GRCh38/Homo_sapiens.GRCh38.109.chrIS_spikes_45S.gtf"
}