Manipulate reaction equations?

[hannahschrenk]

Dear authors,

is it possible (and if so, how?) to manipulate reaction equations of single models? For example, I would like to increase the amount of EX_val_L(e) being produced by Clostridium (SIHUMI community), hence, identify the reaction in which it is produced and increase its output/efficiency.

Thank you very much and best regards,

Hannah

[jotech]

Hi,
You can manipulate model reactions with sybil by creating new reactions (addReact) or directly changing the stoichiometric matrix. But if you want to maximize the production of a certain compound, such as valine, then it might make sense to set the optimization objective accordingly. (changeObjFunc in sybil) (see sybil)