diff --git a/examples/platforms/simulation/openthread-core-simulation-config.h b/examples/platforms/simulation/openthread-core-simulation-config.h
index 517b5b5b257..1b702788a0c 100644
--- a/examples/platforms/simulation/openthread-core-simulation-config.h
+++ b/examples/platforms/simulation/openthread-core-simulation-config.h
@@ -124,7 +124,7 @@
 #endif
 
 #ifndef OPENTHREAD_CONFIG_LOG_PREPEND_UPTIME
-#define OPENTHREAD_CONFIG_LOG_PREPEND_UPTIME 1
+#define OPENTHREAD_CONFIG_LOG_PREPEND_UPTIME !OPENTHREAD_CONFIG_MULTIPLE_INSTANCE_ENABLE
 #endif
 
 #ifndef OPENTHREAD_CONFIG_SRP_CLIENT_BUFFERS_MAX_SERVICES
diff --git a/src/posix/platform/openthread-core-posix-config.h b/src/posix/platform/openthread-core-posix-config.h
index a837ddc960e..cfe1bc0ab88 100644
--- a/src/posix/platform/openthread-core-posix-config.h
+++ b/src/posix/platform/openthread-core-posix-config.h
@@ -137,7 +137,7 @@
 #endif
 
 #ifndef OPENTHREAD_CONFIG_UPTIME_ENABLE
-#define OPENTHREAD_CONFIG_UPTIME_ENABLE 1
+#define OPENTHREAD_CONFIG_UPTIME_ENABLE !OPENTHREAD_CONFIG_MULTIPLE_INSTANCE_ENABLE
 #endif
 
 #ifndef OPENTHREAD_CONFIG_LOG_PREPEND_UPTIME