diff --git a/config.yml b/config.yml new file mode 100644 index 0000000..b72628e --- /dev/null +++ b/config.yml @@ -0,0 +1,38 @@ +all: + project_dir: "/home/danielsg/16S_QIIME2/test" + mux_dir: "multiplexed_fastq" + mapping: "test_mapping_file.tsv" + admin_email: "danielsg@email.chop.edu" + +#try revcomp if you are getting 0 reads when demultiplexing +demux: + mismatch: 0 + revcomp: false + +denoise: + trim_left_f: 0 + trunc_len_f: 240 + trim_left_r: 0 + trunc_len_r: 240 + n_threads: 6 + +taxonomy: + classifier_fp: "/home/danielsg/16S_QIIME2/QIIME2_data/gg-13-8-99-nb-classifier.qza" + +diversity: + sampling_depth: 30 + +unassign: + unassigner_species_fp: "/home/danielsg/16S_QIIME2/unassign_data/unassigner_species.fasta" + +report: + study_group_var: "investigator" + min_reads: 5 + richness_subsample_size: 20 + rmd: "/home/danielsg/16S_QIIME2/scripts/Basic_Bioinformatics_Report.Rmd" + R_helper: "/home/danielsg/16S_QIIME2/scripts/helper_functions.R" + report_rdata: "/home/danielsg/16S_QIIME2/test/report.RData" # rmd can only have one output so have to put this here + +dada2: + rscript: "/home/danielsg/16S_QIIME2/scripts/dada2.R" + species_training_set: "/home/danielsg/16S_QIIME2/dada2_data/rdp_species_assignment_16.fa.gz"