MetaX is a novel tool for linking peptide sequences with taxonomic and functional information in Metaproteomics. We introduce the Operational Taxon-Function (OTF) concept to explore microbial roles and interactions ("who is doing what and how") within ecosystems.
MetaX also features statistical modules and plotting tools for analyzing peptides, taxa, functions, proteins, and taxon-function contributions across groups.
Operational Taxon-Function (OTF) Unit: An operational unit which represents the association between specific taxa and biological functions.
The Tools in OTF Analyzer
Linking Taxa and Functions in different levels of the hierarchy, and various functional categories. e.g., Species-KO, Genus-CAZy, Phylum-EC, etc. Lots of visualization tools can be selected.
Network
Dots represent taxa and squares represent functions in the network.
Heatmap
Show all Taxa of a function.
Bar
Show all functions of a taxon
OTF Heatmap
Show OTFS intensity in groups(samples), e.g., Species-KO OTF Heatmap
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Desktop Version (Recommended)
The desktop version comes fully set up and ready to use, including all required dependencies and a graphical user interface.
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Command-line version
Use PyPi to install, then type
metaxin the terminal to launch the GUI.python -m pip install MetaXTools
Desktop Version(desktop):- Refer to the MetaX Cookbook for detailed instructions on how to use MetaX with the graphical user interface.
Command-line version:- Read the example documentation in the Notebook for detailed instructions and examples.
If you use MetaX in your research, please cite the following publication:
MetaX: A peptide centric metaproteomic data analysis platform using Operational Taxa-Functions (OTF). Wu, Q., Ning, Z., Zhang, A., Zhang, X., Sun, Z., & Figeys, D. (2024). bioRxiv. DOI: 10.1101/2024.04.19.590315.