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It looks like you wrote the output to a log file: rmats_analysis.log
That file should have a section showing how many reads were used or filtered for various reasons. This post has some examples of events not being detected due to reads being filtered out: #89
python "/data2/Home/rmats-turbo-master/rmats.py"
--b1 lymphedema.sorted.bam.list
--b2 normal.sorted.bam.list
--gtf "/data2/Home/E-MTAB-13019data/00.ref/hg38.refGene.gtf"
-t paired
--readLength 151
--nthread 20
--od "/data2/Home/E-MTAB-13019data/lianxi2/"
--tmp "/data2/Home/E-MTAB-13019data/lianxi2/temp/"
2>&1 | tee "/data2/Home/E-MTAB-13019data/lianxi2/rmats_analysis.log",this is my code,however,
in the sumarry.txt
EventType EventTypeDescription TotalEventsJC TotalEventsJCEC SignificantEventsJC SigEventsJCSample1HigherInclusion SigEventsJCSample2HigherInclusion SignificantEventsJCEC SigEventsJCECSample1HigherInclusion SigEventsJCECSample2HigherInclusion
SE skipped exon 0 0 0 0 0 0 0 0
A5SS alternative 5' splice sites 0 0 0 0 0 0 0 0
A3SS alternative 3' splice sites 0 0 0 0 0 0 0 0
MXE mutually exclusive exons 0 0 0 0 0 0 0 0
RI retained intron 0 0 0 0 0 0 0 0
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