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Mini intronic vector sequence for N6 Igg antibody
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Mini intronic vector sequence for N6 Igg antibody
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Full Sequence:
1 tcgcg cgttt cggtg atgac ggtga aaacc tctga cacat gcagc tcccg
51 gagac ggtca cagct tgtct gtaag cggat gccgg gagca gacaa gcccg
101 tcagg gcgcg tcagc gggtg ttggc gggtg tcggg gctgg cttaa ctatg
151 cggca tcaga gcaga ttgta ctgag agtgc accat atgcg gtgtg aaata
201 ccgca cagat gcgta aggag aaaat accgc atcag gcgcc attcg ccatt
251 caggc tgcgc aactg ttggg aaggg cgatc ggtgc gggcc tcttc gctat
301 tacgc cagct ggcga aaggg ggatg tgctg caagg cgatt aagtt gggta
351 acgcc agggt tttcc cagtc acgac gttgt aaaac gacgg ccagt gaatt
401 cgagc tcggt acctc gcgaa tgcat ctaga gcggc cgcat aactt cgtat
451 agcat acatt atacg aacgg taacc tgggt accca tagag cccac cgcat
501 cccca gcatg cctgc tattg tcttc ccaat cctcc ccctt gctgt cctgc
551 cccac cccac ccccc agaat agaat gacac ctact cagac aatgc gatgc
601 aattt cctca tttta ttagg aaagg acagt gggag tggca ccttc caggg
651 tcaag gaagg cacgg gggag gggca aacaa cagat ggctg gcaac tagaa
701 ggcac agcag atctg atcct catca tttac ctgga gacag ggaga ggctc
751 ttctg cgtgt agtgg ttgtg cagag cctca tgcat cacgg agcat gagaa
801 gacgt tcccc tgctg ccacc tgctc ttgtc cacgg tgagc ttgct gtaga
851 ggaag aagga gccat cagag tccag cacgg gaggc gtggt cttgt agttg
901 ttctc cggct gccca ttgct ctccc actcc acagc gatgt cgctg ggata
951 gaagc ctttg accag gcagg tcagg ctgac ctggt tcttg gtcag ctcat
1001 cccgg gatgg gggca gggtg tacac ctgtg gttct cgggg ctgcc ctttg
1051 gcttt ggaga tggtt ttctc gatgg gggct gggag ggctt tgttg ctgac
1101 cttgc acttg tactc cttgc cattc agcca gtcct ggtgc aggac ggtga
1151 ggaca ctgac cacac ggtac gtgct gttgt actgc tcctc cctcg gcttt
1201 gtctt ggcat tatgc acctc cactc catcc acgta ccagt tgaac ttgac
1251 ctcag ggtct tcatg gctca catcc accac cacgc atgtg acctc agggg
1301 tccgg gagat catga gggtg tcctt gggtt ttggg gggaa gagga agact
1351 gacgg tcccc ccagg agttc aggtg ctggg cacgg tgggc atgtg tgagt
1401 tttgt cacaa gattt gggct caact ttctt gtcca ccttg gtgtt gctgg
1451 gcttg tgatt cacgt tgcag atgta ggtct gggtg cccaa gctgc tggag
1501 ggcac ggtca ccacg ctgct gaggg agtag agtcc tgagg actgt aggac
1551 agccg ggaag gtgtg cacgc cgctg gtcag ggctc ctgag ttcca cgaca
1601 cagtc accgg ttcgg ggaag tagtc cttga ccagg cagcc caggg ccgct
1651 gtgcc cccag aggtg ctctt ggagg agggt gccag gggga agaca cttgg
1701 gccct tggta ctcgc cgcag agacg acgac agtcg ttccc tgtcc ccagg
1751 catct aaggc ccaag aggag tctcc atagg accta tctct cgcac agtaa
1801 tagac agccg tgtca tcagg tttaa ggcct ctgat gtcca tgtac gcaat
1851 ctctc tatat acatc tcgtg tcaat gtgac cctgt cccga aaacc accac
1901 caaaa ttcac agccc catat tgtgg cttga tccac cccac ccact caagt
1951 cctcg cccag gggcc tgtcg gaacc aaaat aatat gtggg cggta aaggt
2001 gtatc cagag gtctg gcagg agact cttac tgagg ctccc ggttt cttca
2051 tcgca gtccc tgatt gtacc aggtg cgctc gagaa tgtac accgg ttgca
2101 gttgc tacta gaaaa aggat gatac atgac catcc catgg tggtc tagtg
2151 cagtt acgat atctg atcac acgtg tcgag gacag tgact gcaga ttgct
2201 ttgaa ttagc ggtgg ttttc acaac accta aaaaa gggtt taaaa gatac
2251 ctttg aaccg ctaag aagcc cgaga attag ctccg ctcaa aactc aaggg
2301 gacaa attcc aaaaa tgact tccag cgcca ggctg gcctg actag tctcc
2351 accca ccaaa tgtga acaaa ctcca acgcc attac atccc ctccc cccgc
2401 cgcga ctagc cgtgc tcaaa agccc gaggt gacta ttgcg gccga tagga
2451 ccacg gggtc acagg aagca gcagc cggtg aggga ccagg ccctc ttcct
2501 ttgtg tgggt gactc acccg cccgc tccac cgggc tgccg cgtcc tccat
2551 tttga gctcc ttgca acagg gcccg ggagc ggcca tcttt ccacg cacgc
2601 aactg gtgcc ggacg ggatg gcctc accct agtta gggag gcagg gcaac
2651 gcggc gccgc caagc cagat cgtgc cgggt gctgg ggcca catgg cctcg
2701 gcacg ctaac cccag cctgg ttgct tcggg aaaaa cccca ggcct cgccc
2751 catcc aggtg gcgtc ggaca tgtgc tccga aggcg ggcgg gcccc agccg
2801 ccact cctgt ccctc cattc ctccc caacc atgac ctctc cgggc tccgg
2851 gcgag caagc cccgc accct ccctt tgtta gcccc tattg ctgaa cggca
2901 atcga aggca gcagg gcaac aacaa caaaa aaaaa aaaga ccaga gtgcg
2951 gccgg agtag cacgc ggcgg cggcg cggac accac gctag gcctc aagcc
3001 ggaca cgagg cgagg ctacg gggtt gccgc taggc ctcgc actct gcctc
3051 ccgcg ccgcc cgcaa ctcga agcgg gaatg ctcgc agcta atccc cgccg
3101 acgac agcgg ggccc ggccc gctcg gagca ggacc tccag ctcgg cggcc
3151 cgcgg aagcc acacc cgccc ctcac ctgcg ttctg acggc aaacc cgttg
3201 cgaaa aagaa cgtcc aaggc gactg ccgca cttat atacc gttct ccccc
3251 accct cggga agagg gcgga gccag cacac gacac cgctt tccca gtttg
3301 ccccg cgcca gctcc ccggt cctcc ccccc acgac caggg tctat gagct
3351 tgtgt gctct gctct ccccg cgctc agccc tgagc gcatg ctcct cgcgc
3401 tgtcg tgggg cggct acagg gattt gggtc tgtga gaatt taggg acggt
3451 ccctg ggctc tcccc ttgcg aaggc gatcc ctcct tttgt atgaa ttact
3501 ctcgg ctcca ctagt gggcc ctgca aggaa ttctg ttagg gtgtg gaaag
3551 tcccc aggct cccca gcagg cagaa gtatg caaag catgc atctc aatta
3601 gtcag caacc aggtg tggaa agtcc ccagg ctccc cagca ggcag aagta
3651 tgcaa agcat gcatc tcaat tagtc agcaa ccata gtccc ctagg gagac
3701 atgtt tacat aactt acggt aaatg gcccg cctgg ctgac cgccc aacga
3751 ccccc gccca ttgac gtcaa taatg acgta tgttc ccata gtaac gccaa
3801 taggg acttt ccatt gacgt caatg ggtgg agtat ttacg gtaaa ctgcc
3851 cactt ggcag tacat caagt gtatc atatg ccaag tacgc cccct attga
3901 cgtca atgac ggtaa atggc ccgcc tggca ttatg cccag tacat gacct
3951 tatgg gactt tccta cttgg cagta catct acgta ttagt catcg ctatt
4001 accat gaatt ggttt gatct gatta taact agtgg agaag agcat gcttg
4051 agggc tgagt gcccc tcagt gggca gagag cacat ggccc acagt ccctg
4101 agaag ttggg gggag gggtg ggcaa ttgaa ctggt gccta gagaa ggtgg
4151 ggctt gggta aactg ggaaa gtgat gtggt gtact ggctc cacct ttttc
4201 cccag ggtgg gggag aacca tatat aagtg cagta gtctc tgtga acatt
4251 cgcta gcaca cgtgt gatca gatat catgg ctgct cttag ccacc atggg
4301 atggt catgt atcat ccttt ttcta gtagc aactg caacc ggtgt acatt
4351 catac atcca cgtga cccag tctcc gtcct ccctg tctgt gtcta ttgga
4401 gacag agtca ccatc aactg ccaga cgagt caggg tgttg gcagt gacct
4451 acatt ggtat caaca caaac cgggg agagc cccta aactc ttgat ccacc
4501 atacc tcttc tgtgg aagac ggtgt cccct caaga ttcag cggct ctgga
4551 tttca cacat ctttt aatct gacca tcagc gacct acagg ctgac gacat
4601 tgcca catat tactg tcaag tttta caatt ctttg gccga gggag tagac
4651 tccat attaa acgta cggtg gctgc accat ctgtc ttcat cttcc cacca
4701 tctga tgagc agttg aaatc tggaa ctgcc tctgt tgtgt gcctg ctgaa
4751 taact tctac cccag agaag ccaaa gtgca gtgga aggtg gacaa tgccc
4801 tccag agcgg aaaca gccag gaatc tgtga cagag cagga ttcca aggat
4851 tccac ataca gcctg agcag cacac tgaca ctgtc caagg ctgac tacga
4901 gaagc acaag gtgta cgcct gcgaa gtgac acacc aggga ctgtc ctccc
4951 ctgtg acaaa gagct tcaac agagg agaat gctga tagga taagc tttga
5001 tctaa ataaa cttat aaatt gtgag agaaa ttaat gaatg tctaa gttaa
5051 tgcag aaacg gagag acata ctata ttcat gaact aaaag actta atatt
5101 gtgaa ggtat acttt ctttc cacat aaatt tgtag tcaat atgtt caccc
5151 caaaa aagct gtttg ttaac ttgcc aacct cattc taaaa tgtat ataga
5201 agccc aaaag acaat aacaa aaata ttctt gtaga acaaa atggg aaaga
5251 atgtt ccact aaata tcaag attta gagca aagca tgaga tgtgt gggga
5301 tagac agtga ggctg ataaa ataga gtaga gctca gaaac agacc cattg
5351 atata tgtaa gtgac ctatg aaaaa aatat ggcat tttac aatgg gaaaa
5401 tgatg atctt tttct ttttt agaaa aacag ggaaa tatat ttata tgtaa
5451 aaaat aaaag ggaac ccata tgtca tacca tacac acaaa aaaat tccag
5501 tgaat tataa gtcta aatgg agaag gcaaa acttt aaatc tttta gaaaa
5551 taata tagaa gcatg ccatc atgac ttcag tgtag agaaa aattt cttat
5601 gactc aaagt cctaa ccaca aagaa aagat tgtta attag attgc atgaa
5651 tatta agact tattt ttaaa attaa aaaac catta agaaa agtca ggcca
5701 tagaa tgaca gaaaa tattt gcaac acccc agtaa agaga attgt aatat
5751 gcaga ttata aaaag aagtc ttaca aatca gtaaa aaata aaact agaca
5801 aaaat ttgaa cagat gaaag agaaa ctcta aataa tcatt acaca tgaga
5851 aactc aatct cagaa atcag agaac tatca ttgca tatac actaa attag
5901 agaaa tatta aaagg ctaag taaca tctgt ggcaa tattg atggt atata
5951 acctt gatat gatgt gatga gaaca gtact ttacc ccatg ggctt cctcc
6001 ccaaa ccctt acccc agtat aaatc atgac aaata tactt taaaa accat
6051 taccc tatat ctaac cagta ctcct caaaa ctgtc aaggt catca aaaat
6101 aagaa aagtc tgagg aactg tcaaa actaa gagga accca aggag acatg
6151 agaat tatat gtaat gtggc attct gaatg agatc ccaga acaga aaaag
6201 aacag tagct aaaaa actaa tgaaa tataa ataaa gtttg aactt tagtt
6251 ttttt taaaa aagag tagca ttaac acggc aaagc cattt tcata ttttt
6301 cttga acatt aagta caagt ctata attaa aaatt tttta aatgt agtct
6351 ggaac attgc cagaa acaga agtac agcag ctatc tgtgc tgtcg cctaa
6401 ctatc catag ctgat tggtc taaaa tgaga tacat caacg ctcct ccatg
6451 ttttt tgttt tcttt ttaaa tgaaa aactt tattt tttaa gagga gtttc
6501 aggtt catag caaaa ttgag aggaa ggtac attca agctg aggaa gtttt
6551 cctct attcc tagtt tactg agaga ttgca tcatg aatgg gtgtt aaatt
6601 ttgtc aaatg ctttt tctgt gtcta tcaat atgac catgt gattt tcttc
6651 tttaa cctgt tgatg ggaca aatta cgtta attga ttttc aaacg ttgaa
6701 ccacc cttac atatc tggaa taaat tctac ttggt tgtgg tgtat atttt
6751 ttgat acatt cttgg attct ttttg ctaat atttt gttga aaatg tttgt
6801 atctt tgttc atgag agata ttggt ctgtt gtttt ctttt cttgt aatgt
6851 cattt tctag ttccg gtatt aaggt aatgc tggcc tagtt gaatg attta
6901 ggaag tattc cctct gcttc tgtct tctga aagag attgt agaaa gttga
6951 tacaa ttttt ttttc tttaa atatc ttgat agaat tggat cctat caatc
7001 gtcga ctgta ccttg ggcct gggac cggaa ctgaa acata aaatg aatgc
7051 aactg ttgtt gttaa cttgt ttatt gcagc ttata atggt tacaa ataaa
7101 gcaat agcat cacaa atttc acaaa taaag cattt ttttc actgc attct
7151 agttg tggtt tgtcc aaact catca atgta tctta tcatg tctgg atcac
7201 gcgta gtcta gatac cgttc gtata gcata catta tacga agtta tgttt
7251 cggcc ggtgg atcca ccggt attgg gatct tcaca cagca ggtaa ggttg
7301 cgggc cgggc ctggg ccggg tccgg gccgg gtatt gcccg cctaa tgagc
7351 gggct ttttt ttctt acccc ttctt ccgct tcctc gctca ctgac tcgct
7401 gcgct cggtc gttcg gctgc ggcga gcggt atcag ctcac tcaaa ggcgg
7451 taata cggtt atcca cagaa tcagg ggata acgca ggaaa gaaca tgtga
7501 gcaaa aggcc agcaa aaggc cagga accgt aaaaa ggccg cgttg ctggc
7551 gtttt tccat aggct ccgcc cccct gacga gcatc acaaa aatcg acgct
7601 caagt cagag gtggc gaaac ccgac aggac tataa agata ccagg cgttt
7651 ccccc tggaa gctcc ctcgt gcgct ctcct gttcc gaccc tgccg cttac
7701 cggat acctg tccgc ctttc tccct tcggg aagcg tggcg ctttc tcata
7751 gctca cgctg taggt atctc agttc ggtgt aggtc gttcg ctcca agctg
7801 ggctg tgtgc acgaa ccccc cgttc agccc gaccg ctgcg cctta tccgg
7851 taact atcgt cttga gtcca acccg gtaag acacg actta tcgcc actgg
7901 cagca gccac tggta acagg attag cagag cgagg tatgt aggcg gtgct
7951 acaga gttct tgaag tggtg gccta actac ggcta cacta gaaga acagt
8001 atttg gtatc tgcgc tctgc tgaag ccagt tacct tcgga aaaag agttg
8051 gtagc tcttg atccg gcaaa caaac caccg ctggt agcgg tggtt ttttt
8101 gtttg caagc agcag attac gcgca gaaaa aaagg atctc aagaa gatcc
8151 tttga tcttt tctac ggggt ctgac gctca gtgga acgaa aactc acgtt
8201 aaggg atttt ggtca tgaga ttatc aaaaa ggatc ttcac ctaga tcctt
8251 ttaaa ttaaa aatga agttt taaat caatc taaag tatat atgag taaac
8301 ttggt ctgac agtta ccaat gctta atcag tgagg cacct atctc agcga
8351 tctgt ctatt tcgtt catcc atagt tgcct gactc ctgca aacca cgttg
8401 tggta gaatt ggtaa agaga gtcgt gtaaa atatc gagtt cgcac atctt
8451 gttgt ctgat tattg atttt tggcg aaacc atttg atcat atgac aagat
8501 gtgta tctac cttaa cttaa tgatt ttgat aaaaa tcatt aggta ccccg
8551 gcccg cactg acccc tggtg ttgct ttttt ttttt aggcc gcaag ctgaa
8601 gcgtg tccac tagct gacta gctaa ccgcg gaaac agtta ccaat gctta
8651 atcag tgagg cacct atctc agcga tctgt ctatt tcgtt catcc atagt
8701 tgcct gactc cccgt cgtgt agata actac gatac gggag ggctt accat
8751 ctggc cccag tgctg caatg atacc gcgag accca cgctc accgg ctcca
8801 gattt atcag caata aacca gccag ccgga agggc cgagc gcaga agtgg
8851 tcctg caact ttatc cgcct ccatc cagtc tatta attgt tgccg ggaag
8901 ctaga gtaag tagtt cgcca gttaa tagtt tgcgc aacgt tgttg ccatt
8951 gctac aggca tcgtg gtgtc acgct cgtcg tttgg tatgg cttca ttcag
9001 ctccg gttcc caacg atcaa ggcga gttac atgat ccccc atgtt gtgca
9051 aaaaa gcggt tagct ccttc ggtcc tccga tcgtt gtcag aagta agttg
9101 gccgc agtgt tatca ctcat ggtta tggca gcact gcata attct cttac
9151 tgtca tgcca tccgt aagat gcttt tctgt gactg gtgag tactc aacca
9201 agtca ttctg agaat agtgt atgcg gcgac cgagt tgctc ttgcc cggcg
9251 tcaat acggg ataat accgc gccac atagc agaac tttaa aagtg ctcat
9301 cattg gaaaa cgttc ttcgg ggcga aaact ctcaa ggatc ttacc gctgt
9351 tgaga tccag ttcga tgtaa cccac tcgtg caccc aactg atctt cagca
9401 tcttt tactt tcacc agcgt ttctg ggtga gcaaa aacag gaagg caaaa
9451 tgccg caaaa aaggg aataa gggcg acacg gaaat gttga atact catac
9501 tcttc ctttt tcaat attat tgaag cattt atcag ggtta ttgtc tcatg
9551 agcgg ataca tattt gaatg tattt agaaa aataa acaaa taggg gttcc
9601 gcgca cattt ccccg aaaat ttccc cgaaa tccct atcag tgata gagaa
9651 aagtg aaagt cgagt ttacc actcc ctatc agtga tagag aaaag tgaaa
9701 gtcga gttta ccact cccta tcagt gatag agaaa agtga aagtc gagtt
9751 tacca ctccc tatca gtgat agaga aaagt gaaag tcgag tttac cactc
9801 cctat cagtg ataga gaaaa gtgaa agtcg agttt accac tccct atcag
9851 tgata gagaa aagtg aaagt cgagt ttacc actcc ctatc agtga tagag
9901 aagtg ccacc tgacg tctaa gaaac catta ttatc atgac attaa cctat
9951 aaaaa taggc gtatc acgag gccct ttcgt c
bGH polysignal (A)
Location: 483-707bp
Length: 225bp
Sequence:
483 ccata gagcc caccg catcc ccagc atgcc tgcta ttgtc ttccc aatcc
533 tcccc cttgc tgtcc tgccc caccc caccc cccag aatag aatga cacct
583 actca gacaa tgcga tgcaa tttcc tcatt ttatt aggaa aggac agtgg
633 gagtg gcacc ttcca gggtc aagga aggca cgggg gaggg gcaaa caaca
683 gatgg ctggc aacta gaagg cacag
Heavy Chain Constant Region (3’-5’ direction)
Location: 726-1715
Length:990
Sequence:
726 tttac ctgga gacag ggaga ggctc ttctg cgtgt agtgg ttgtg cagag
776 cctca tgcat cacgg agcat gagaa gacgt tcccc tgctg ccacc tgctc
826 ttgtc cacgg tgagc ttgct gtaga ggaag aagga gccat cagag tccag
876 cacgg gaggc gtggt cttgt agttg ttctc cggct gccca ttgct ctccc
926 actcc acagc gatgt cgctg ggata gaagc ctttg accag gcagg tcagg
976 ctgac ctggt tcttg gtcag ctcat cccgg gatgg gggca gggtg tacac
1026 ctgtg gttct cgggg ctgcc ctttg gcttt ggaga tggtt ttctc gatgg
1076 gggct gggag ggctt tgttg ctgac cttgc acttg tactc cttgc cattc
1126 agcca gtcct ggtgc aggac ggtga ggaca ctgac cacac ggtac gtgct
1176 gttgt actgc tcctc cctcg gcttt gtctt ggcat tatgc acctc cactc
1226 catcc acgta ccagt tgaac ttgac ctcag ggtct tcatg gctca catcc
1276 accac cacgc atgtg acctc agggg tccgg gagat catga gggtg tcctt
1326 gggtt ttggg gggaa gagga agact gacgg tcccc ccagg agttc aggtg
1376 ctggg cacgg tgggc atgtg tgagt tttgt cacaa gattt gggct caact
1426 ttctt gtcca ccttg gtgtt gctgg gcttg tgatt cacgt tgcag atgta
1476 ggtct gggtg cccaa gctgc tggag ggcac ggtca ccacg ctgct gaggg
1526 agtag agtcc tgagg actgt aggac agccg ggaag gtgtg cacgc cgctg
1576 gtcag ggctc ctgag ttcca cgaca cagtc accgg ttcgg ggaag tagtc
1626 cttga ccagg cagcc ca
Heavy Chain Variable Region (3’-5’ direction)
Location: 1716-2081
Length:366
Sequence:
1716 cgcag agacg acgac agtcg ttccc tgtcc ccagg catct aaggc ccaag
1766 aggag tctcc atagg accta tctct cgcac agtaa tagac agccg tgtca
1816 tcagg tttaa ggcct ctgat gtcca tgtac gcaat ctctc tatat acatc
1866 tcgtg tcaat gtgac cctgt cccga aaacc accac caaaa ttcac agccc
1916 catat tgtgg cttga tccac cccac ccact caagt cctcg cccag gggcc
1966 tgtcg gaacc aaaat aatat gtggg cggta aaggt gtatc cagag gtctg
2016 gcagg agact cttac tgagg ctccc ggttt cttca tcgca gtccc tgatt
2066 gtacc aggtg cgctc g
mEF-1a intron/promoter
Location: 2195-3527
Length: 1333bp
Sequence
2195 attgc tttga attag cggtg gtttt cacaa cacct aaaaa agggt ttaaa
2245 agata ccttt gaacc gctaa gaagc ccgag aatta gctcc gctca aaact
2295 caagg ggaca aattc caaaa atgac ttcca gcgcc aggct ggcct gacta
2345 gtctc caccc accaa atgtg aacaa actcc aacgc catta catcc cctcc
2395 ccccg ccgcg actag ccgtg ctcaa aagcc cgagg tgact attgc ggccg
2445 atagg accac ggggt cacag gaagc agcag ccggt gaggg accag gccct
2495 cttcc tttgt gtggg tgact caccc gcccg ctcca ccggg ctgcc gcgtc
2545 ctcca ttttg agctc cttgc aacag ggccc gggag cggcc atctt tccac
2595 gcacg caact ggtgc cggac gggat ggcct caccc tagtt aggga ggcag
2645 ggcaa cgcgg cgccg ccaag ccaga tcgtg ccggg tgctg gggcc acatg
2695 gcctc ggcac gctaa cccca gcctg gttgc ttcgg gaaaa acccc aggcc
2745 tcgcc ccatc caggt ggcgt cggac atgtg ctccg aaggc gggcg ggccc
2795 cagcc gccac tcctg tccct ccatt cctcc ccaac catga cctct ccggg
2845 ctccg ggcga gcaag ccccg caccc tccct ttgtt agccc ctatt gctga
2895 acggc aatcg aaggc agcag ggcaa caaca acaaa aaaaa aaaag accag
2945 agtgc ggccg gagta gcacg cggcg gcggc gcgga cacca cgcta ggcct
2995 caagc cggac acgag gcgag gctac ggggt tgccg ctagg cctcg cactc
3045 tgcct cccgc gccgc ccgca actcg aagcg ggaat gctcg cagct aatcc
3095 ccgcc gacga cagcg gggcc cggcc cgctc ggagc aggac ctcca gctcg
3145 gcggc ccgcg gaagc cacac ccgcc cctca cctgc gttct gacgg caaac
3195 ccgtt gcgaa aaaga acgtc caagg cgact gccgc actta tatac cgttc
3245 tcccc caccc tcggg aagag ggcgg agcca gcaca cgaca ccgct ttccc
3295 agttt gcccc gcgcc agctc cccgg tcctc ccccc cacga ccagg gtcta
3345 tgagc ttgtg tgctc tgctc tcccc gcgct cagcc ctgag cgcat gctcc
3395 tcgcg ctgtc gtggg gcggc tacag ggatt tgggt ctgtg agaat ttagg
3445 gacgg tccct gggct ctccc cttgc gaagg cgatc cctcc ttttg tatga
3495 attac tctcg gctcc actag tgggc cctgc aag
mEF-1a intron
Location:2228-3175
Length: 948bp
Sequence :
2228 ctaaa aaagg gttta aaaga tacct ttgaa ccgct aagaa gcccg agaat
2278 tagct ccgct caaaa ctcaa gggga caaat tccaa aaatg acttc cagcg
2328 ccagg ctggc ctgac tagtc tccac ccacc aaatg tgaac aaact ccaac
2378 gccat tacat cccct ccccc cgccg cgact agccg tgctc aaaag cccga
2428 ggtga ctatt gcggc cgata ggacc acggg gtcac aggaa gcagc agccg
2478 gtgag ggacc aggcc ctctt ccttt gtgtg ggtga ctcac ccgcc cgctc
2528 caccg ggctg ccgcg tcctc cattt tgagc tcctt gcaac agggc ccggg
2578 agcgg ccatc tttcc acgca cgcaa ctggt gccgg acggg atggc ctcac
2628 cctag ttagg gaggc agggc aacgc ggcgc cgcca agcca gatcg tgccg
2678 ggtgc tgggg ccaca tggcc tcggc acgct aaccc cagcc tggtt gcttc
2728 gggaa aaacc ccagg cctcg cccca tccag gtggc gtcgg acatg tgctc
2778 cgaag gcggg cgggc cccag ccgcc actcc tgtcc ctcca ttcct cccca
2828 accat gacct ctccg ggctc cgggc gagca agccc cgcac cctcc ctttg
2878 ttagc cccta ttgct gaacg gcaat cgaag gcagc agggc aacaa caaca
2928 aaaaa aaaaa agacc agagt gcggc cggag tagca cgcgg cggcg gcgcg
2978 gacac cacgc taggc ctcaa gccgg acacg aggcg aggct acggg gttgc
3028 cgcta ggcct cgcac tctgc ctccc gcgcc gcccg caact cgaag cggga
3078 atgct cgcag ctaat ccccg ccgac gacag cgggg cccgg cccgc tcgga
3128 gcagg acctc cagct cggcg gcccg cggaa gccac acccg cccct cac
hCMV Enhancer
Location 3705-4027
Length:323bp
Sequence:
3705 ttaca taact tacgg taaat ggccc gcctg gctga ccgcc caacg acccc
3755 cgccc attga cgtca ataat gacgt atgtt cccat agtaa cgcca atagg
3805 gactt tccat tgacg tcaat gggtg gagta tttac ggtaa actgc ccact
3855 tggca gtaca tcaag tgtat catat gccaa gtacg ccccc tattg acgtc
3905 aatga cggta aatgg cccgc ctggc attat gccca gtaca tgacc ttatg
3955 ggact ttcct acttg gcagt acatc tacgt attag tcatc gctat tacca
4005 tgaat tggtt tgatc tgatt ata
hEF-1a promoter core
Location: 4034-4251
Length: 218
Sequence:
4034 ggaga agagc atgct tgagg gctga gtgcc cctca gtggg cagag agcac
4084 atggc ccaca gtccc tgaga agttg ggggg agggg tgggc aattg aactg
4134 gtgcc tagag aaggt ggggc ttggg taaac tggga aagtg atgtg gtgta
4184 ctggc tccac ctttt tcccc agggt ggggg agaac catat ataag tgcag
4234 tagtc tctgt gaaca ttc
Light Chain Variable Region (5’-3’)
Location:4353-4661
Length:309
Sequence:
4353 tacat ccacg tgacc cagtc tccgt cctcc ctgtc tgtgt ctatt ggaga
4403 cagag tcacc atcaa ctgcc agacg agtca gggtg ttggc agtga cctac
4453 attgg tatca acaca aaccg gggag agccc ctaaa ctctt gatcc accat
4503 acctc ttctg tggaa gacgg tgtcc cctca agatt cagcg gctct ggatt
4553 tcaca catct tttaa tctga ccatc agcga cctac aggct gacga cattg
4603 ccaca tatta ctgtc aagtt ttaca attct ttggc cgagg gagta gactc
4653 catat taaa
Light Chain Constant Region
Location: 4668-4982
Length:315
Sequence:
4668 gtggc tgcac catct gtctt catct tccca ccatc tgatg agcag ttgaa
4718 atctg gaact gcctc tgttg tgtgc ctgct gaata acttc taccc cagag
4768 aagcc aaagt gcagt ggaag gtgga caatg ccctc cagag cggaa acagc
4818 cagga atctg tgaca gagca ggatt ccaag gattc cacat acagc ctgag
4868 cagca cactg acact gtcca aggct gacta cgaga agcac aaggt gtacg
4918 cctgc gaagt gacac accag ggact gtcct cccct gtgac aaaga gcttc
4968 aacag aggag aatgc
Scaffolding Matrix attachment Region
Location: 5004-6981
Length: 1978
Sequence:
5004 aaata aactt ataaa ttgtg agaga aatta atgaa tgtct aagtt aatgc
5054 agaaa cggag agaca tacta tattc atgaa ctaaa agact taata ttgtg
5104 aaggt atact ttctt tccac ataaa tttgt agtca atatg ttcac cccaa
5154 aaaag ctgtt tgtta acttg ccaac ctcat tctaa aatgt atata gaagc
5204 ccaaa agaca ataac aaaaa tattc ttgta gaaca aaatg ggaaa gaatg
5254 ttcca ctaaa tatca agatt tagag caaag catga gatgt gtggg gatag
5304 acagt gaggc tgata aaata gagta gagct cagaa acaga cccat tgata
5354 tatgt aagtg accta tgaaa aaaat atggc atttt acaat gggaa aatga
5404 tgatc ttttt ctttt ttaga aaaac aggga aatat attta tatgt aaaaa
5454 ataaa aggga accca tatgt catac catac acaca aaaaa attcc agtga
5504 attat aagtc taaat ggaga aggca aaact ttaaa tcttt tagaa aataa
5554 tatag aagca tgcca tcatg acttc agtgt agaga aaaat ttctt atgac
5604 tcaaa gtcct aacca caaag aaaag attgt taatt agatt gcatg aatat
5654 taaga cttat tttta aaatt aaaaa accat taaga aaagt caggc catag
5704 aatga cagaa aatat ttgca acacc ccagt aaaga gaatt gtaat atgca
5754 gatta taaaa agaag tctta caaat cagta aaaaa taaaa ctaga caaaa
5804 atttg aacag atgaa agaga aactc taaat aatca ttaca catga gaaac
5854 tcaat ctcag aaatc agaga actat cattg catat acact aaatt agaga
5904 aatat taaaa ggcta agtaa catct gtggc aatat tgatg gtata taacc
5954 ttgat atgat gtgat gagaa cagta cttta cccca tgggc ttcct cccca
6004 aaccc ttacc ccagt ataaa tcatg acaaa tatac tttaa aaacc attac
6054 cctat atcta accag tactc ctcaa aactg tcaag gtcat caaaa ataag
6104 aaaag tctga ggaac tgtca aaact aagag gaacc caagg agaca tgaga
6154 attat atgta atgtg gcatt ctgaa tgaga tccca gaaca gaaaa agaac
6204 agtag ctaaa aaact aatga aatat aaata aagtt tgaac tttag ttttt
6254 tttaa aaaag agtag catta acacg gcaaa gccat tttca tattt ttctt
6304 gaaca ttaag tacaa gtcta taatt aaaaa ttttt taaat gtagt ctgga
6354 acatt gccag aaaca gaagt acagc agcta tctgt gctgt cgcct aacta
6404 tccat agctg attgg tctaa aatga gatac atcaa cgctc ctcca tgttt
6454 tttgt tttct tttta aatga aaaac tttat ttttt aagag gagtt tcagg
6504 ttcat agcaa aattg agagg aaggt acatt caagc tgagg aagtt ttcct
6554 ctatt cctag tttac tgaga gattg catca tgaat gggtg ttaaa ttttg
6604 tcaaa tgctt tttct gtgtc tatca atatg accat gtgat tttct tcttt
6654 aacct gttga tggga caaat tacgt taatt gattt tcaaa cgttg aacca
6704 ccctt acata tctgg aataa attct acttg gttgt ggtgt atatt ttttg
6754 ataca ttctt ggatt ctttt tgcta atatt ttgtt gaaaa tgttt gtatc
6804 tttgt tcatg agaga tattg gtctg ttgtt ttctt ttctt gtaat gtcat
6854 tttct agttc cggta ttaag gtaat gctgg cctag ttgaa tgatt tagga
6904 agtat tccct ctgct tctgt cttct gaaag agatt gtaga aagtt gatac
6954 aattt ttttt tcttt aaata tcttg ata
SV40 Poly-signal (a)
Location: 7064-7198
Length: 135
Sequence:
7064 aactt gttta ttgca gctta taatg gttac aaata aagca atagc atcac
7114 aaatt tcaca aataa agcat ttttt tcact gcatt ctagt tgtgg tttgt
7164 ccaaa ctcat caatg tatct tatca tgtct ggatc
Intronic Region
Location 7251-8631
Length:1381
Sequence:
7251 Cggcc ggtgg atcca ccggt attgg gatct tcaca cagca ggtaa ggttg
7301 cgggc cgggc ctggg ccggg tccgg gccgg gtatt gcccg cctaa tgagc
7351 gggct ttttt ttctt acccc ttctt ccgct tcctc gctca ctgac tcgct
7401 gcgct cggtc gttcg gctgc ggcga gcggt atcag ctcac tcaaa ggcgg
7451 taata cggtt atcca cagaa tcagg ggata acgca ggaaa gaaca tgtga
7501 gcaaa aggcc agcaa aaggc cagga accgt aaaaa ggccg cgttg ctggc
7551 gtttt tccat aggct ccgcc cccct gacga gcatc acaaa aatcg acgct
7601 caagt cagag gtggc gaaac ccgac aggac tataa agata ccagg cgttt
7651 ccccc tggaa gctcc ctcgt gcgct ctcct gttcc gaccc tgccg cttac
7701 cggat acctg tccgc ctttc tccct tcggg aagcg tggcg ctttc tcata
7751 gctca cgctg taggt atctc agttc ggtgt aggtc gttcg ctcca agctg
7801 ggctg tgtgc acgaa ccccc cgttc agccc gaccg ctgcg cctta tccgg
7851 taact atcgt cttga gtcca acccg gtaag acacg actta tcgcc actgg
7901 cagca gccac tggta acagg attag cagag cgagg tatgt aggcg gtgct
7951 acaga gttct tgaag tggtg gccta actac ggcta cacta gaaga acagt
8001 atttg gtatc tgcgc tctgc tgaag ccagt tacct tcgga aaaag agttg
8051 gtagc tcttg atccg gcaaa caaac caccg ctggt agcgg tggtt ttttt
8101 gtttg caagc agcag attac gcgca gaaaa aaagg atctc aagaa gatcc
8151 tttga tcttt tctac ggggt ctgac gctca gtgga acgaa aactc acgtt
8201 aaggg atttt ggtca tgaga ttatc aaaaa ggatc ttcac ctaga tcctt
8251 ttaaa ttaaa aatga agttt taaat caatc taaag tatat atgag taaac
8301 ttggt ctgac agtta ccaat gctta atcag tgagg cacct atctc agcga
8351 tctgt ctatt tcgtt catcc atagt tgcct gactc ctgca aacca cgttg
8401 tggta gaatt ggtaa agaga gtcgt gtaaa atatc gagtt cgcac atctt
8451 gttgt ctgat tattg atttt tggcg aaacc atttg atcat atgac aagat
8501 gtgta tctac cttaa cttaa tgatt ttgat aaaaa tcatt aggta ccccg
8551 gcccg cactg acccc tggtg ttgct ttttt ttttt aggcc gcaag ctgaa
8601 gcgtg tccac tagct gacta gctaa ccgcg g
AmpR
Location:8638-9429
Length:861
Sequence:
8638 ttacc aatgc ttaat cagtg aggca cctat ctcag cgatc tgtct atttc
8688 gttca tccat agttg cctga ctccc cgtcg tgtag ataac tacga tacgg
8738 gaggg cttac catct ggccc cagtg ctgca atgat accgc gagac ccacg
8788 ctcac cggct ccaga tttat cagca ataaa ccagc cagcc ggaag ggccg
8838 agcgc agaag tggtc ctgca acttt atccg cctcc atcca gtcta ttaat
8888 tgttg ccggg aagct agagt aagta gttcg ccagt taata gtttg cgcaa
8938 cgttg ttgcc attgc tacag gcatc gtggt gtcac gctcg tcgtt tggta
8988 tggct tcatt cagct ccggt tccca acgat caagg cgagt tacat gatcc
9038 cccat gttgt gcaaa aaagc ggtta gctcc ttcgg tcctc cgatc gttgt
9088 cagaa gtaag ttggc cgcag tgtta tcact catgg ttatg gcagc actgc
9138 ataat tctct tactg tcatg ccatc cgtaa gatgc ttttc tgtga ctggt
9188 gagta ctcaa ccaag tcatt ctgag aatag tgtat gcggc gaccg agttg
9238 ctctt gcccg gcgtc aatac gggat aatac cgcgc cacat agcag aactt
9288 taaaa gtgct catca ttgga aaacg ttctt cgggg cgaaa actct caagg
9338 atctt accgc tgttg agatc cagtt cgatg taacc cactc gtgca cccaa
9388 ctgat cttca gcatc tttta ctttc accag cgttt ctggg tg
AmpT Promoter
Location:9499-9603
Length: 105
Sequence:
9499 actct tcctt tttca atatt attga agcat ttatc agggt tattg tctca
9549 tgagc ggata catat ttgaa tgtat ttaga aaaat aaaca aatag gggtt
9599 ccgcg
Tetracycline Response Element repeats
Location:9650-9901
Length:251
Sequence:
9650 tccct atcag tgata gagaa aagtg aaagt cgagt ttacc actcc ctatc
9700 agtga tagag aaaag tgaaa gtcga gttta ccact cccta tcagt gatag
9750 agaaa agtga aagtc gagtt tacca ctccc tatca gtgat agaga aaagt
9800 gaaag tcgag tttac cactc cctat cagtg ataga gaaaa gtgaa agtcg
9850 agttt accac tccct atcag tgata gagaa aagtg aaagt cgagt ttacc
9900 actcc ctatc agtga tagag a